Stress-Responsive Fibroblast Transfer of Mitochondria-Enriched Cargo Drives Osteosarcoma Migration and Chemoresistance

Hans Zoellner1,2,3, Duan Ni4,5,6, Jhati Kakani1, Jingyi Zhou1, Brigitta Sabrina Auwyang1, Yogambha Ramaswamy3, Belal Chami7*.
1The Cellular and Molecular Pathology Research Unit, Discipline of Oral Pathology and Oral Medicine, Sydney Dental School, Faculty of Medicine and Health, The University of Sydney, Westmead Hospital, NSW, 2145, Australia
2Strongarch Ptyt Ltd, Pennant Hills, NSW, 2120, Australia
3Graduate School of Biomedical Engineering, University of NSW, NSW, 2052, Australia
4Sydney Medical School Nepean, The University of Sydney, Sydney, NSW, Australia
5Charles Perkins Centre, The University of Sydney, Sydney, NSW, Australia
6Nepean Hospital, Nepean Blue Mountains Local Health District, Sydney, NSW, Australia
7Redox Inflammation Group, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, NSW, 2006, Australia

*Author B.C appears as the senior author of the study.  Correspondence address to Dr Belal Chami (belal.chami@sydney.edu.au), Level 4 West, Charles Perkins Centre, The University of Sydney, NSW 2006, Australia.

Supplementary Figures and Legends

Supplementary Figure 1: Optimisation of MitoTracker Green labelling SAOS-2 cells. Panel shows representative images of SAO2-2 labelled with MitoTracker Green (green) and DAPI (blue) in addition to an overlay of both images (composition) of two conditions, labelled cells are condition media. Labelled cells were SAOS-2 and were intentionally stained with MitoTracker Green while the condition media condition followed x 7 HBSS wash cycles. The final HBSS wash media was used to overlay on unlabelled SAOS-2 to ensure there was no residual dye remaining. All cell nucleic where stained with DAPI. Image were taken using Lecia WideField under GFP and DAPI channels. Magnification x 20. Scale bar = 50 µm.