Project info 1

Project info 1 Experiment Date2019-11-13 Experiment ID Notebook ID Project Experimenter Protocol TNF-α（pg/ml） NC 27.17 29.48 33.96 26.5 30.64 30.45 Model 117.92 117.69 95.51 102.44 111.67 115.8 Cur-Low 88.92 92.82 89 90.98 86.58 93.45 Cur-Mid 73.94 76.51 75.49 71.77 70.63 70.45 Cur-High 44.93 46.04 44.67 43.52 47.53 49.51

IL-1β（pg/ml） NC 9.4 9.38 9.84 9.99 10.01 9.7 Model 35.49 36.42 35.22 36.07 35.48 36.01 Cur-Low 29.45 29.53 30.35 29.55 30.52 30.43 Cur-Mid 22.31 22.09 22.54 21.72 22.58 22.18 Cur-High 14.37 14.44 15.38 15.01 13.97 14.61

IL-6（pg/ml） NC 36.28 33.63 35.93 33.35 32.81 35.38 Model 121.48 125.7 123.49 119.24 118.82 126.58 Cur-Low 97.29 105.8 101.41 97.69 104.35 103.4 Cur-Mid 65.42 64.8 74.49 71.78 70.15 71.1 Cur-High 46.83 44.33 45.07 47.37 39.18 47.6

Percentages of collagen areas NC 3.68 2.1 2.79 2.3 3.59 3.33 2.27 3.95 3.52 Model 52.01 56.59 55.98 59.25 51.33 52.77 52.7 50.23 59.8 Cur-Low 42.63 40.59 48.8 44.6 45.59 41.93 44.71 40.79 49.64 Cur-Mid 34.55 32.42 29.48 31.86 26.27 28.55 34.42 33.21 34.61 Cur-High 9.36 9.53 9.23 9.02 9.24 8.36 5.89 9.74 9.77

TUNEL NC 2.26 0.4 1.2 1.85 0.74 1.14 0.58 2.57 0.8 Model 21.6 22.71 23.31 21.66 24.71 23.62 23.84 20.47 24.14 Cur-Low 17.24 16.27 16.69 16.81 16.99 17.55 17.79 17.53 17.78 Cur-Mid 11.28 10.49 10.53 10.7 10.48 11.19 11.85 11.38 10.93 Cur-High 2.21 2.2 3.71 3.62 2.02 3.11 2.75 2.94 3.26

TLR4 mRNA NC 1 0.99 1 Model 13.91 14.01 13.96 Cur-Low 10.83 11.08 10.91 Cur-Mid 7.89 8.04 7.91 Cur-High 4.91 5.05 5.06

NF-κB（p65） NC 1 1.07 1.1 Model 12.89 13.16 12.99 Cur-Low 10.05 9.92 10.12 Cur-Mid 6.91 6.96 7.13 Cur-High 4.14 3.99 3.96

TLR4 IHC NC 0.77 0.5 1.26 1.28 1.25 0.96 0.72 0.2 0.34 Model 13.79 13.43 14.99 13.62 13.96 14.69 14 13.69 13.07 Cur-Low 12.75 11.28 11.91 12.49 12.74 11.45 11.18 11.13 11.09 Cur-Mid 7.38 7.45 7.44 7.93 8.42 7.24 7.13 7.85 7.61 Cur-High 1.39 2.1 2.99 1.07 2.18 1.52 2.57 2.32 2.48

NF-κB(p65) IHC NC 0.44 1.73 0.17 1.3 0.85 1.44 1.37 1.23 1.51 Model 13.81 13.4 14.11 14.98 13.19 13.91 13.27 14.98 14.24 Cur-Low 12.77 11.26 12.21 12.26 11.97 12.86 12.13 12.71 11.72 Cur-Mid 8.31 8.79 7.02 7.14 7.81 7.94 7.91 7.69 7.4 Cur-High 2.31 2.65 1.76 1.32 2.82 2.87 1.22 2.48 1.03

p-NF-κB(p65) IHC NC 0.29 0.18 0.64 0.34 0.64 0.66 1.18 0.4 1.93 Model 14.02 14.37 14.64 13.91 14.32 13.77 13.19 13.02 14.29 Cur-Low 11.2 12.12 12.32 12.87 11.48 12.46 11.24 12.22 12.55 Cur-Mid 7.94 7.06 8.99 7 8.77 8.02 7.04 7.68 8.99 Cur-High 2.18 1.34 2.5 1.66 2.94 1.95 2.4 2.94 2.21

TLR4 WB NC 0.02 0.02 0.01 Model 0.59 0.62 0.5 Cur-Low 0.41 0.53 0.39 Cur-Mid 0.3 0.31 0.28 Cur-High 0.09 0.11 0.15

NF-κB(p65) WB NC 0.27 0.3 0.25 Model 0.87 0.79 0.73 Cur-Low 0.68 0.67 0.61 Cur-Mid 0.5 0.56 0.58 Cur-High 0.29 0.43 0.45

p-NF-κB(p65) WB NC 0.21 0.07 0.14 Model 0.99 0.97 0.91 Cur-Low 0.89 0.93 0.81 Cur-Mid 0.72 0.89 0.71 Cur-High 0.39 0.46 0.55