Project info 1

Project info 1 Experiment Date2019-11-14 Experiment ID Notebook ID Project Experimenter Protocol TNF-α（pg/ml） NC 8.79 10.62 12.86 13.27 12.02 12.13 Model 86.38 85.19 83.05 80.85 80.03 78.84 Cru 26.7 26.5 26.63 29.19 33.19 30.92 si-NC 81.44 79.89 86.32 81.09 82.24 81.84 si-TLR4 26.76 31.42 26.3 27.76 28.14 29.39 Cur+TLR4 86.23 78.21 83.64 81.51 78.44 85.4

IL-1β（pg/ml） NC 4.48 4.68 4.27 4.47 4.73 4.46 Model 30.22 28.34 28.38 28.75 29.74 28.31 Cru 12.33 13.92 13.07 9.78 14.87 9.49 si-NC 28.99 29.59 29.24 29.61 30.55 30.35 si-TLR4 13.51 14.41 10.75 12.84 12.81 14.48 Cur+TLR4 28.21 29.68 29.89 28.74 29.78 28.81

IL-6（pg/ml） NC 22.01 23.26 20.53 23.27 23.66 23.84 Model 94.87 99.22 99.2 97.24 97.77 97.21 Cru 38.64 40.46 39.64 39.93 46.63 45.99 si-NC 95.87 94.34 100.83 98.58 97.15 101.88 si-TLR4 48.44 40.53 48.36 43.97 44.09 43.97 Cur+TLR4 99.14 97.49 95.93 98.27 101.01 98.8

Hyp（ng/ml） NC 107.9 102.06 98.6 97.22 101.4 103.01 Model 332.96 331.29 368.99 373.59 321.2 343.64 Cru 169.72 155.87 152.71 156.53 158.38 158.32 si-NC 322.87 379.5 360.64 328.6 327.77 332.78 si-TLR4 153.13 166.85 159.51 165.6 167.63 142.45 Cur+TLR4 368.28 355.09 347.69 362.19 331.53 320.43

MTT NC 98.97 100 99.08 100.11 101.03 100.57 Model 38.62 33.22 36.21 35.29 36.67 40 Cru 84.14 79.66 82.41 78.62 86.21 85.29 si-NC 32.64 33.1 29.77 29.31 29.77 30.92 si-TLR4 83.45 78.05 77.59 79.43 86.21 78.51 Cur+TLR4 39.66 37.93 32.3 36.21 38.74 39.08

Apoptosis NC 2.74 2.78 2.61 Model 38.8 39.36 40.5 Cru 17.87 20.02 20.55 si-NC 40.51 42.07 40.57 si-TLR4 20.19 20.73 20.59 Cur+TLR4 40.87 40.88 40.68

TUNEL NC 0 0 0 0 0 0 0 0 0 Model 47.06 48.08 50.98 50.98 52.17 53.06 53.7 48.28 48.72 Cru 18.49 16.88 16.95 15.66 17.48 14.29 16.25 15.22 14.81 si-NC 55.1 52.86 53.13 48.65 53.85 54 53.49 51.16 56.1 si-TLR4 13.04 15 15.09 16.51 16.22 15.6 18.81 20.39 19.35 Cur+TLR4 52.38 55 47.27 52.11 50 46.75 46.15 46.84 51.56

TLR4 mRNA NC 1 1.1 0.99 Model 14.1 14.17 14.18 Cru 6 5.95 6.05 si-NC 14.12 13.9 14.08 si-TLR4 6.1 5.91 6.05 Cur+TLR4 14.02 14.17 14.04

NF-κB(p65) mRNA NC 1 1.06 1.09 Model 14.18 14.1 14.17 Cru 5 5.01 4.98 si-NC 13.04 12.96 13.03 si-TLR4 5.02 5.1 5.06 Cur+TLR4 12.92 13.02 13.1

TLR4 WB NC 0.02 0.06 0.04 Model 0.6 0.64 0.67 Cru 0.28 0.35 0.37 si-NC 0.56 0.61 0.7 si-TLR4 0.29 0.38 0.38 Cur+TLR4 0.55 0.74 0.73

NF-κB(p65) WB NC 0.21 0.16 0.12 Model 0.92 0.68 0.7 Cru 0.58 0.4 0.41 si-NC 0.88 0.69 0.76 si-TLR4 0.47 0.4 0.43 Cur+TLR4 0.83 0.65 0.75

p-NF-κB(p65) WB NC 0.32 0.18 0.39 Model 0.78 0.79 0.88 Cru 0.62 0.65 0.57 si-NC 0.81 0.65 0.94 si-TLR4 0.74 0.6 0.73 Cur+TLR4 0.84 0.74 0.88

Nuclear volume NC 0.43 0.39 0.6 0.07 0.17 0.83 0.32 0.06 1.62 Model 10.96 11.47 11.04 10.05 11.13 10.24 11.65 11.75 11.16 Cru 1.53 2.9 1.58 1.17 2.8 1.89 2.5 2.43 1.17 si-NC 11.67 11.9 11.29 10.82 10.04 11.85 11.45 10.49 11.46 si-TLR4 0.39 1.25 1.23 1.75 1.63 1.07 0.8 1.31 0.78 Cur+TLR4 11.34 10.14 10.25 11.93 11.73 10.66 11.77 10.38 11.82