Project info 1

Project info 1 Experiment Date2019-11-14 Experiment ID Notebook ID Project Experimenter Protocol TNF-α（pg/ml） NC 12.13 11.73 10.48 10.63 14.8 10.98 Model 82.32 79.39 84.22 80.97 79.11 83.83 Cur-Low 62.85 67.3 68.54 63.36 65.84 66.05 Cur-Mid 47.74 44.49 48.05 51.69 45.59 49.51 Cur-High 28.18 33.82 29.5 26.55 28.65 27.45

IL-1β（pg/ml） NC 4.44 4.25 5.06 4.48 4.61 4.41 Model 30.39 30.58 28.75 30.32 30.58 29.86 Cur-Low 24.49 21.6 23.21 24.34 21.65 22.23 Cur-Mid 15.73 17.54 16.35 18.01 17.2 17.34 Cur-High 10.66 10.51 10.72 9.78 10.86 10.72

IL-6（pg/ml） NC 20.09 20.05 22.44 22.88 23.3 22.17 Model 101.01 92.04 95.48 100.65 94.79 94.35 Cur-Low 78.02 84.16 85.88 76.3 83.6 76.71 Cur-Mid 69.15 66.65 68.27 60.05 69.63 65.85 Cur-High 34.49 42.88 42.65 35.47 42.29 38.07

Hyp（ng/ml） NC 104.86 93.47 102.3 103.55 105.4 102.77 Model 346.56 325.56 347.87 325.56 329.2 342.32 Cur-Low 274.13 263.87 287.55 263.45 283.02 284.39 Cur-Mid 219.77 207.25 223.29 217.15 227.47 230.1 Cur-High 149.91 145.55 145.08 154.98 154.56 147.52

MTT NC 0.96 0.99 1 0.99 1.05 1.02 Model 0.38 0.32 0.37 0.34 0.4 0.3 Cur-Low 0.57 0.49 0.52 0.59 0.54 0.62 Cur-Mid 0.65 0.64 0.67 0.71 0.71 0.64 Cur-High 0.81 0.77 0.77 0.79 0.77 0.88

Apoptosis NC 2.15 2.04 2.49 Model 37.46 38.7 38.77 Cur-Low 28.62 29.65 29.93 Cur-Mid 19.47 19.7 19.97 Cur-High 10.6 11.55 11.81

TUNEL NC 0 0 0 0 0 0 0 0 0 Model 46.81 47.27 49.21 51.22 52.63 50 52.94 52.46 53.23 Cur-Low 31.67 36.36 35.21 33.87 32.43 33.33 35.29 28.36 33.33 Cur-Mid 15.38 15.38 16.67 17.57 16 16.25 14.86 14.77 15.57 Cur-High 5.36 2.82 3.39 4.41 2.83 2.78 4.69 2.99 5.48

TLR4 mRNA NC 1 1.04 1.06 Model 13.98 14.16 13.91 Cur-Low 11.13 10.91 10.91 Cur-Mid 8.07 7.95 8.04 Cur-High 5.01 5.06 5

NF-κB（p65）mRNA NC 1 1.04 1 Model 13.09 13.04 12.97 Cur-Low 10.11 10.04 10.07 Cur-Mid 7 7.07 6.91 Cur-High 4.01 4.15 4.08

TLR4 WB NC 0 0.01 0.02 Model 0.24 0.32 0.36 Cur-Low 0.21 0.23 0.27 Cur-Mid 0.09 0.12 0.19 Cur-High 0.05 0.04 0.05

NF-kB WB NC 0.12 0.13 0.13 Model 0.59 0.83 0.78 Cur-Low 0.5 0.63 0.64 Cur-Mid 0.44 0.47 0.46 Cur-High 0.24 0.34 0.3

p-NF-κB(p65) WB NC 0.23 0.29 0.41 Model 0.95 0.84 0.99 Cur-Low 0.93 0.78 0.89 Cur-Mid 0.79 0.66 0.82 Cur-High 0.55 0.6 0.75

Nuclear volume NC 1.4 0.35 0.6 1.59 1.6 0.78 0.13 1.26 1.45 Model 11.99 11.52 10.86 10.05 11.17 11.5 10.01 11.99 10.41 Cur-Low 8.02 9.91 9.77 9.86 9.5 9.84 8 8.8 9.23 Cur-Mid 5.29 5.21 5.21 6.36 5.16 6.64 6.95 6.03 6.29 Cur-High 2.48 2.73 2.92 1.94 1.29 1.54 1.76 2.28 1.07