{"text_format":"3","workbook":"xlsx","n_sheets":10,"sheets":["Table S1","Table S2","Table S3","Table S4","Table S5","Table S6","Table S7","Table S8","Fig S1","Fig S2 SSD histo"],"not_rendered":{"images":3},"read":"whole","date_system":"1900"}

{"sheet":"Table S1","index":0,"n_rows":39,"n_cols":9,"rows_kept":39}
["Table S1: Wild mice used for the analyses of sexual size dimorphism of salivary (SMG) and lacrimal (LAC) glands; number of tested animals in each sex for each locality of both subspecies, their distance from the center of the hybrid zone, mean relative size of glands with standard error of the mean are indicated for each locality, sex and subspecies separately. Subspecies specific origine of tested mice was controled using several X and Y chromosome markers acording to Macholán et al. 2011."]
[]
["Locality","Dist","N","sex","Subspecies","mean SMG","SE  SMG","mean LAC","SE  LAC"]
["Domesticus",null,25,"F","DD",0.003668577158122916,6.8884629671919165E-4,7.6981680622707858E-4,1.85396636202239E-4]
["Weickenreuth22",-50.471808167085,1,"F","DD",3.8141335914811227E-3,0,4.6950629235237176E-4,0]
["Straas",-45.3857602966813,7,"F","DD",3.5424095290721444E-3,7.395316737000945E-4,8.1271166029200979E-4,5.0365634979454708E-5]
["Rieglersreuth",-44.7899324561265,1,"F","DD",0.003276740237691002,0,6.7911714770797966E-4,0]
["Mussen 3",-44.0443030995582,5,"F","DD",3.3867742584566063E-3,6.4703765257324657E-4,5.6985992307205025E-4,6.2989001371036236E-5]
["Benk",-38.2793587521729,2,"F","DD",3.5077062861757977E-3,2.9445416610579924E-5,9.7259613227442031E-4,4.3854040472024035E-4]
["Albertsreuth",-37.3115064783834,2,"F","DD",3.4793923933238253E-3,6.0146191636343692E-4,7.1107481191115291E-4,2.4575956075912654E-5]
["Finkenmühle",-33.1925698594303,1,"F","DD",0.003834355828220859,0,5.6237218813905935E-4,0]
["Birk 4",-31.8729529370433,6,"F","DD",0.004180710656815439,1.1996177178952821E-3,9.3813356695034752E-4,2.6509164634994282E-4]
["Musculus",null,16,"F","MM",0.005017279033543659,0.000959257082924198,8.4448809976109132E-4,2.428911682593411E-4]
["Přílezy",33.8444016087876,1,"F","MM",0.005658093797276853,0,9.455370650529501E-4,0]
["Kojšovice",37.949437166201,1,"F","MM",0.004719033232628398,0,4.4712990936555892E-4,0]
["Buškovice p Brázdová",43.4322009090494,4,"F","MM",0.004722128603202884,4.5022881435394235E-4,7.5758049010058121E-4,1.1859743925312358E-4]
["Stvolny",52.1871004893926,4,"F","MM",0.005356022993084302,1.4460602636187873E-3,7.0240734414361156E-4,7.346902874713168E-5]
["Petrohrad, Statek, Velká garáž",53.9238603902471,1,"F","MM",0.005007530120481928,0,9.1867469879518087E-4,0]
["Pastuchovice",56.0809511473137,1,"F","MM",0.006962899050905953,0,2.1311475409836068E-3,0]
["Žihle statek",58.5635549641988,4,"F","MM",4.4040754875641665E-3,7.9110772461091032E-4,8.0734226125084817E-4,2.4210454757563451E-4]
["Domesticus",null,31,"M","DD",0.004292852008704845,0.000877808524000698,1.1239227091212564E-3,2.53688282657438E-4]
["Weickenreuth22",-50.471808167085,1,"M","DD",0.004413012729844412,0,6.2234794908062234E-4,0]
["Straas",-45.3857602966813,14,"M","DD",0.00425293548500342,1.1904975026932362E-3,1.2097917773626376E-3,3.5982204042602606E-4]
["Rieglersreuth",-44.7899324561265,5,"M","DD",0.004359653544425392,5.2479474327245344E-4,1.1429390099466168E-3,1.299762119270122E-4]
["Mussen",-44.0443030995582,3,"M","DD",0.003799498163091382,3.8749939191706615E-4,9.6731294324637652E-4,4.5354911804538827E-5]
["Benk",-38.2793587521729,1,"M","DD",3.937007874015748E-3,0,1.3143549364021804E-3,0]
["Finkenmühle",-33.1925698594303,2,"M","DD",0.004669877761231075,3.9139674857284739E-4,1.083783196823033E-3,2.1368515760734674E-4]
["Birk 4",-31.8729529370433,5,"M","DD",0.004530155428415779,9.9075393039328437E-4,1.0367231882161875E-3,9.0426176153836314E-5]
["Musculus",null,26,"M","MM",5.2938622120862295E-3,8.5442211457821679E-4,1.15452047083069E-3,2.8562715167970444E-4]
["Přílezy",33.8444016087876,1,"M","MM",0.00502283105022831,0,0.000665362035225049,0]
["Kojšovice",37.949437166201,3,"M","MM",0.00525286906810549,8.113201187504443E-4,0.001087475351317642,1.6336110264649861E-4]
["Buškovice statek",43.4322009090494,4,"M","MM",0.005300306412314481,2.0421963464202454E-4,8.7819256469826219E-4,3.7876445731142372E-4]
["Žlutice",44.6699578537734,1,"M","MM",0.005558312655086849,0,1.5818858560794043E-3,0]
["Libyně",46.1640276083434,1,"M","MM",6.4039408866995075E-3,0,1.2931034482758623E-3,0]
["Stvolny",52.1871004893926,2,"M","MM",0.006373568927178067,3.6602175736674594E-4,1.0716555039213859E-3,2.33919654864782E-4]
["Vroutek",53.67,1,"M","MM",6.9241982507288625E-3,0,1.2244897959183673E-3,0]
["Petrohrad, Statek, Velká garáž",53.9238603902471,4,"M","MM",5.356240107293778E-3,6.4983973108543973E-4,1.2885447787810058E-3,2.1414254790071764E-4]
["Pastuchovice",56.0809511473137,2,"M","MM",6.2640505076181855E-3,9.5855940166304256E-4,1.5097019810898665E-3,3.2517139635883613E-5]
["Žihle statek",58.5635549641988,7,"M","MM",0.004295871788450923,7.8322456832148634E-4,1.1658001011723928E-3,3.4307267452451121E-4]

{"sheet":"Table S2","index":1,"n_rows":29,"n_cols":8,"rows_kept":29}
["Table S2: Mice from wild derived strains representing both subspecies used for the analyses of sexual size dimorphism of salivary (SMG) and lacrimal (LAC) glands; number of tested animals of each sex for each strain of both subspecies, mean relative size of glands with standard error of the mean are indicated for each strain, sex and subspecies separately. "]
[]
["Subspecies","Strain","Sex","N SMG (LAC)  if different","mean SMG","SE  SMG","mean LAC","SE  LAC"]
["DOM","n=5","F",38,{"v":4.4880201671094215E-3,"d":"0.00449"},{"v":7.6443487501214285E-4,"d":"0.00076"},{"v":9.0345146021391721E-4,"d":"0.00090"},{"v":2.9464306403191256E-4,"d":"0.00029"}]
["Dom","DDO","F",3,{"v":0.004578305228115386,"d":"0.00458"},{"v":7.6010006123136177E-4,"d":"0.00076"},{"v":0.001001668363965244,"d":"0.00100"},{"v":2.4479932610271826E-4,"d":"0.00024"}]
["Dom","DROS","F",12,{"v":4.3226148631848125E-3,"d":"0.00432"},{"v":6.2575991899349386E-4,"d":"0.00063"},{"v":5.3168761014438519E-4,"d":"0.00053"},{"v":1.1505401217731532E-4,"d":"0.00012"}]
["Dom","SCHUNT","F",3,{"v":0.004700087951493774,"d":"0.00470"},{"v":2.7814628373157231E-4,"d":"0.00028"},{"v":9.5353359624559923E-4,"d":"0.00095"},{"v":2.1867127226831676E-4,"d":"0.00022"}]
["Dom","STRA","F",17,{"v":0.004053565079519745,"d":"0.00405"},{"v":4.4696081418709414E-4,"d":"0.00045"},{"v":1.1828949032199527E-3,"d":"0.00118"},{"v":1.1484817687970895E-4,"d":"0.00011"}]
["Dom","WLA","F",3,{"v":0.007309200700425733,"d":"0.00731"},{"v":1.1012986321486107E-3,"d":"0.00110"},{"v":6.5869497700816613E-4,"d":"0.00066"},{"v":1.57716862174736E-4,"d":"0.00016"}]
["DOM","n=5","M","42 (41)",{"v":0.005325005273809573,"d":"0.00533"},{"v":7.0398747375494087E-4,"d":"0.00070"},{"v":1.6476378844697416E-3,"d":"0.00165"},{"v":5.5074230717987735E-4,"d":"0.00055"}]
["Dom","DDO","M","3 (2)",{"v":0.00441585977137749,"d":"0.00442"},{"v":3.7690490573331323E-4,"d":"0.00038"},{"v":1.4396172549692183E-3,"d":"0.00144"},{"v":3.4658577283267758E-5,"d":"0.00003"}]
["Dom","DROS","M",11,{"v":5.2652220375380785E-3,"d":"0.00527"},{"v":5.7602999491504242E-4,"d":"0.00058"},{"v":9.7378819575292543E-4,"d":"0.00097"},{"v":1.4308520354920339E-4,"d":"0.00014"}]
["Dom","SCHUNT","M",3,{"v":0.00595238066505598,"d":"0.00595"},{"v":2.8271082655050017E-4,"d":"0.00028"},{"v":1.0455809122797675E-3,"d":"0.00105"},{"v":8.8049229089113e-05,"d":"0.00009"}]
["Dom","STRA","M",22,{"v":5.0549383845139365E-3,"d":"0.00505"},{"v":3.9890879481261238E-4,"d":"0.00040"},{"v":0.002160829579796445,"d":"0.00216"},{"v":1.7276249451075211E-4,"d":"0.00017"}]
["Dom","WLA","M",3,{"v":0.007806471106158705,"d":"0.00781"},{"v":2.7160553483997034E-4,"d":"0.00027"},{"v":1.0957517025592274E-3,"d":"0.00110"},{"v":2.5154149829018367E-5,"d":"0.00003"}]
["MUS","n=6","F","46 (45)",{"v":0.004835026334951219,"d":"0.00484"},{"v":6.4713594849065924E-4,"d":"0.00065"},{"v":1.0259535804415122E-3,"d":"0.00103"},{"v":3.4300681915636782E-4,"d":"0.00034"}]
["Mus","BUSNA","F",21,{"v":0.004618727343974153,"d":"0.00462"},{"v":5.4660481113608615E-4,"d":"0.00055"},{"v":6.982886044654442E-4,"d":"0.00070"},{"v":1.380258395840188E-4,"d":"0.00014"}]
["Mus","MBK","F",12,{"v":5.1631685191813265E-3,"d":"0.00516"},{"v":6.6954962323889731E-4,"d":"0.00067"},{"v":1.2695190168818728E-3,"d":"0.00127"},{"v":2.265831503431079E-4,"d":"0.00023"}]
["Mus","MDH","F",4,{"v":5.4802518208083625E-3,"d":"0.00548"},{"v":7.8285279111514768E-4,"d":"0.00078"},{"v":0.001449961580366237,"d":"0.00145"},{"v":2.4890106180349179E-4,"d":"0.00025"}]
["Mus","MPB","F","3 (2)",{"v":0.005134445424528885,"d":"0.00513"},{"v":2.7812944789637359E-4,"d":"0.00028"},{"v":0.001577645312437313,"d":"0.00158"},{"v":2.6201109414822745E-4,"d":"0.00026"}]
["Mus","PWD","F",3,{"v":0.004850709810997426,"d":"0.00485"},{"v":8.4561584200652936E-4,"d":"0.00085"},{"v":1.2330344712956508E-3,"d":"0.00123"},{"v":1.8022594917503884E-4,"d":"0.00018"}]
["Mus","STUF","F",3,{"v":3.8611473214368625E-3,"d":"0.00386"},{"v":0.000416475907388278,"d":"0.00042"},{"v":1.2051272877615727E-3,"d":"0.00121"},{"v":3.1954667533975697E-4,"d":"0.00032"}]
["MUS","n=6","M",48,{"v":5.478604135860936E-3,"d":"0.00548"},{"v":7.1170982758728216E-4,"d":"0.00071"},{"v":1.6732884522561748E-3,"d":"0.00167"},{"v":2.6121712610185059E-4,"d":"0.00026"}]
["Mus","BUSNA","M",22,{"v":0.006083654007507288,"d":"0.00608"},{"v":4.0855070312581678E-4,"d":"0.00041"},{"v":1.4310632821805628E-3,"d":"0.00143"},{"v":1.9362223685515909E-4,"d":"0.00019"}]
["Mus","MBK","M",12,{"v":0.005232815545561073,"d":"0.00523"},{"v":5.3576685994101533E-4,"d":"0.00054"},{"v":1.7789906634330798E-3,"d":"0.00178"},{"v":1.6039011248272688E-4,"d":"0.00016"}]
["Mus","MDH","M",3,{"v":0.004743370644040933,"d":"0.00474"},{"v":7.3229740932379418E-4,"d":"0.00073"},{"v":2.2483759031805156E-3,"d":"0.00225"},{"v":2.5787815324446341E-4,"d":"0.00026"}]
["Mus","MPB","M",4,{"v":0.00482093480223283,"d":"0.00482"},{"v":2.4214887795335214E-4,"d":"0.00024"},{"v":0.002116173251110536,"d":"0.00212"},{"v":2.754889558400813E-4,"d":"0.00028"}]
["Mus","PWD","M",3,{"v":0.005296177220563134,"d":"0.00530"},{"v":4.225597054836475E-4,"d":"0.00042"},{"v":0.001978250137616814,"d":"0.00198"},{"v":8.9396195640646461E-5,"d":"0.00009"}]
["Mus","STUF","M",4,{"v":0.00423411025167205,"d":"0.00423"},{"v":2.9970771876856276E-4,"d":"0.00030"},{"v":0.001585498603073227,"d":"0.00159"},{"v":9.0913132588527968E-5,"d":"0.00009"}]

{"sheet":"Table S3","index":2,"n_rows":13,"n_cols":4,"rows_kept":13}
[null,"Table S3: Preparation of protein standards for the BCA microassay using the Protein assay standard BSA set."]
[]
[null,"Final concentration (mg/ml)","Standard stock solution (ml)","Normal saline (ml)"]
[null,null," standard 2000 mg/ml"]
[null,200,100,900]
[null,40,20,980]
[null,20,10,990]
[null,10,5,995]
[null,null," standard 500 mg/ml"]
[null,5,10,990]
[null,"2025-05-02",5,995]
[null,null," standard 125 mg/ml"]
[null,1,8,992]

{"sheet":"Table S4","index":3,"n_rows":45,"n_cols":8,"rows_kept":45}
[]
[]
[null,"Table S4: Results of statistical tests of the difference in relative gland size (SMG or LAC) in wild mice by factors sex and subspecies; complete results of Factorial ANOVA and Post Hoc comparisons by Tukey HSD test are indicated separately for salivary gland (SMG) and Lacrimal gland (LAC). "]
[]
[null,"A) Factorial ANOVA: dependent variable relSMG, factors: sex, subspecies and interaction between the two factors, statisticaly significant values (at p<0,05) are indicated in blod. "]
[]
[null,null,"Factorial ANOVA"]
[null,"rel SMG","SS","df","MS","F","p"]
[null,"Intercept",{"v":1.9275348963896971E-3,"d":"0.001928"},1,{"v":1.9275348963896971E-3,"d":"0.001928"},{"v":1675.715747805206,"d":"1675.716"},{"v":0,"d":"0.000000"}]
[null,"sex",{"v":4.6850655161858662E-6,"d":"0.000005"},1,{"v":4.6850655161858662E-6,"d":"0.000005"},{"v":4.072993998540065,"d":"4.073"},{"v":0.046423924580434206,"d":"0.046424"}]
[null,"subspecies",{"v":3.1873683799982232E-5,"d":"0.000032"},1,{"v":3.1873683799982232E-5,"d":"0.000032"},{"v":27.709606702443615,"d":"27.710"},{"v":8.87371145230631e-07,"d":"0.000001"}]
[null,"subs. X sex",{"v":6.9789733221538966E-7,"d":"0.000001"},1,{"v":6.9789733221538966E-7,"d":"0.000001"},{"v":0.6067218560530446,"d":"0.607"},{"v":0.43798128114797885,"d":"0.437981"}]
[null,"error",{"v":1.0812590410870436E-4,"d":"0.000108"},94,{"v":1.1502755756245145E-6,"d":"0.000001"}]
[]
[null,"B) Posthoc comparison by Tukey HSD test of relative gland size per interaction of factors subspecies and sex, statisticaly significant values (at p<0,05) are indicated in blod. "]
[]
[null,"Tukey HSD test;"]
[null," variable rel SMG "]
[null,"df = 94"]
[null,null,"Factors"]
[null,null,"subspecies","sex","1 (0,00429)","2 (0,00529)","3 (0,00367)","4 (0,00502)"]
[null,1,"Dom","M",null,{"v":3.892181596867772E-3,"d":"0.003892"},{"v":0.14065648709566148,"d":"0.140656"},{"v":0.13243014898197414,"d":"0.132430"}]
[null,2,"Mus","M",{"v":3.892181596867772E-3,"d":"0.003892"},null,{"v":1.4213254824768651E-4,"d":"0.000142"},{"v":0.8489097984375867,"d":"0.848910"}]
[null,3,"Dom","F",{"v":0.14065648709566148,"d":"0.140656"},{"v":1.4213254824768651E-4,"d":"0.000142"},null,{"v":0.001032054318110709,"d":"0.001032"}]
[null,4,"Mus","F",{"v":0.13243014898197414,"d":"0.132430"},{"v":0.8489097984375867,"d":"0.848910"},{"v":0.001032054318110709,"d":"0.001032"}]
[]
[null,"C) Factorial ANOVA: dependent variable relLAC, factors: sex, subspecies and interaction between the two factors, statisticaly significant values (at p<0,05) are indicated in blod. "]
[null,null,"Factorial ANOVA"]
[null,"rel LAC","SS","df","MS","F","p"]
[null,"Intercept",{"v":8.7481367005235052E-5,"d":"0.000087"},1,{"v":8.7481367005235052E-5,"d":"0.000087"},{"v":652.9036895892739,"d":"652.9037"},{"v":0,"d":"0.000000"}]
[null,"sex",{"v":2.5463610316694543E-6,"d":"0.000003"},1,{"v":2.5463610316694543E-6,"d":"0.000003"},{"v":19.004372811227878,"d":"19.0044"},{"v":3.3326806249789342E-5,"d":"0.000033"}]
[null,"subspecies",{"v":6.39741334199721e-08,"d":"0.000000"},1,{"v":6.39741334199721e-08,"d":"0.000000"},{"v":0.4774610774620922,"d":"0.4775"},{"v":0.49127748356536616,"d":"0.491277"}]
[null,"subs. X sex",{"v":1.1213951389454431E-8,"d":"0.000000"},1,{"v":1.1213951389454431E-8,"d":"0.000000"},{"v":0.0836935965645281,"d":"0.0837"},{"v":0.7729900940757173,"d":"0.772990"}]
[null,"error",{"v":1.2594887469031067E-5,"d":"0.000013"},94,{"v":1.3398816456416029E-7,"d":"0.000000"}]
[]
[null,"D) Posthoc comparison by Tukey HSD test of relative LAC gland size per interaction of factors subspecies and sex, statisticaly significant values (at p<0,05) are indicated in blod. "]
[null,"Tukey HSD test;"]
[null," variable rel LAC "]
[null,"df = 94"]
[null,null,"Factors"]
[null,null,"subspecies","sex","1 (0,00112)","2 (0,00115)","3 (0,00077)","4 (0,00084)"]
[null,1,"Dom","M",null,{"v":0.9892581444998616,"d":"0.989258"},{"v":0.002934250468535904,"d":"0.002934"},{"v":0.06975607131434469,"d":"0.069756"}]
[null,2,"Mus","M",{"v":0.9892581444998616,"d":"0.989258"},null,{"v":1.7960582437832606E-3,"d":"0.001796"},{"v":0.0441480033787016,"d":"0.044148"}]
[null,3,"Dom","F",{"v":0.002934250468535904,"d":"0.002934"},{"v":1.7960582437832606E-3,"d":"0.001796"},null,{"v":0.9197999649414892,"d":"0.919800"}]
[null,4,"Mus","F",{"v":0.06975607131434469,"d":"0.069756"},{"v":0.04414800337870162,"d":"0.044148"},{"v":0.9197999649414892,"d":"0.919800"}]

{"sheet":"Table S5","index":4,"n_rows":44,"n_cols":8,"rows_kept":44}
[]
[null,"WDS strains"]
[null,"Table S5: Results of statistical tests of the difference in relative gland size (SMG or LAC) in WDS mice by factors sex and subspecies, complete results of Factorial ANOVA and Post Hoc comparisons by Tukey HSD test are indicated separately for salivary gland (SMG) and Lacrimal gland (LAC). "]
[]
[null,"A) Factorial ANOVA: dependent variable relSMG, factors: sex, subspecies and interaction between the two factors, statisticaly significant values (at p<0,05) are indicated in blod. "]
[]
[null,null,"Factorial ANOVA"]
[null,"rel SMG","SS","df","MS","F","p"]
[null,"Intercept",{"v":0.004312266873116809,"d":"0.004312"},1,{"v":0.004312266873116809,"d":"0.004312"},{"v":4908.403448172736,"d":"4908.403"},{"v":0,"d":"0.000000"}]
[null,"subspecies",{"v":2.3297939308531065E-6,"d":"0.000002"},1,{"v":2.3297939308531065E-6,"d":"0.000002"},{"v":2.6518693996009413,"d":"2.652"},{"v":0.10530202592253313,"d":"0.105302"}]
[null,"sex",{"v":2.4162786445281023E-5,"d":"0.000024"},1,{"v":2.4162786445281023E-5,"d":"0.000024"},{"v":27.50309936633326,"d":"27.503"},{"v":4.6685540511237633E-7,"d":"0.000000"}]
[null,"subs. X sex",{"v":3.0832688310524419E-7,"d":"0.000000"},1,{"v":3.0832688310524419E-7,"d":"0.000000"},{"v":0.3509506208052208,"d":"0.351"},{"v":0.554371999474923,"d":"0.554372"}]
[null,"error",{"v":1.4759602431485555E-4,"d":"0.000148"},168,{"v":8.78547763778902e-07,"d":"0.000001"}]
[]
[null,"B) Posthoc comparison - Tukey HSD test of relative SMG gland size per interactipn of factors subspecies and sex, statisticaly significant values (at p<0,05) are indicated in blod. "]
[]
[null,"Tukey HSD test;"]
[null," variable rel SMG "]
[null,"df = 170"]
[null,null,"Factors"]
[null,null,"subspecies","sex","1 (0,00449)","2 (0,00533)","3 (0,00484)","4 (0,00548)"]
[null,1,"Dom","F",null,{"v":4.0993918129128559E-4,"d":"0.000410"},{"v":0.3294963118728965,"d":"0.329496"},{"v":1.397422495941214E-5,"d":"0.000014"}]
[null,2,"Dom","M",{"v":4.0993918129128559E-4,"d":"0.000410"},null,{"v":0.0681192438187026,"d":"0.068119"},{"v":0.8655214390007007,"d":"0.865521"}]
[null,3,"Mus","F",{"v":0.3294963118728965,"d":"0.329496"},{"v":0.0681192438187026,"d":"0.068119"},null,{"v":0.004858083584331485,"d":"0.004858"}]
[null,4,"Mus","M",{"v":1.397422495941214E-5,"d":"0.000014"},{"v":0.8655214390007007,"d":"0.865521"},{"v":0.004858083584331485,"d":"0.004858"}]
[null,"C) Factorial ANOVA: dependent variable relLAC, factors: sex, subspecies and interaction between the two factors, statisticaly significant values (at p<0,05) are indicated in blod. "]
[null,null,"Factorial ANOVA"]
[null,"rel LAC","SS","df","MS","F","p"]
[null,"Intercept",{"v":2.9400077245279611E-4,"d":"0.000294"},1,{"v":2.9400077245279611E-4,"d":"0.000294"},{"v":1564.7557195915142,"d":"1564.756"},{"v":0,"d":"0.000000"}]
[null,"subspecies",{"v":2.340960644531694E-7,"d":"0.000000"},1,{"v":2.340960644531694E-7,"d":"0.000000"},{"v":1.245925827782555,"d":"1.246"},{"v":0.2659260076135356,"d":"0.265926"}]
[null,"sex",{"v":2.0651649993139864E-5,"d":"0.000021"},1,{"v":2.0651649993139864E-5,"d":"0.000021"},{"v":109.91395422594007,"d":"109.914"},{"v":0,"d":"0.000000"}]
[null,"subs. X sex",{"v":1.0004334735728748E-7,"d":"0.000000"},1,{"v":1.0004334735728748E-7,"d":"0.000000"},{"v":0.5324591451865328,"d":"0.532"},{"v":0.46659100135373455,"d":"0.466591"}]
[null,"error",{"v":3.1565393341373287E-5,"d":"0.000032"},168,{"v":1.878892460796029E-7,"d":"0.000000"}]
[]
[null,"D) Posthoc comparison - Tukey HSD test of relative LAC gland size per interactipn of factors subspecies and sex, statisticaly significant values (at p<0,05) are indicated in blod. "]
[null,"Tukey HSD test;"]
[null," variable rel LAC "]
[null,"df = 94"]
[null,null,"Factors"]
[null,null,"subspecies","sex","1 (0,00090)","2 (0,00165)","3 (0,000103)","4 (0,00164)"]
[null,1,"Dom","F",null,{"v":7.7145961309144084E-6,"d":"0.000008"},{"v":0.5739304354803783,"d":"0.573930"},{"v":7.7145961148161746E-6,"d":"0.000008"}]
[null,2,"Dom","M",{"v":7.7145961309144084E-6,"d":"0.000008"},null,{"v":7.714669368663607E-6,"d":"0.000008"},{"v":0.9924842561140993,"d":"0.992484"}]
[null,3,"Mus","F",{"v":0.5739304354803783,"d":"0.573930"},{"v":7.714669368663607E-6,"d":"0.000008"},null,{"v":7.714596890195935E-6,"d":"0.000008"}]
[null,4,"Mus","M",{"v":7.7145961148161746E-6,"d":"0.000008"},{"v":0.9924842561140993,"d":"0.992484"},{"v":7.714596890195935E-6,"d":"0.000008"}]

{"sheet":"Table S6","index":5,"n_rows":25,"n_cols":8,"rows_kept":25}
[]
[null,"Histology"]
[null,"Table S6: Results of statistical tests of the diffrence in mean % of GCT in SMG gland  by factors sex and subspecies, complete results of Factorial ANOVA and Post Hoc comparisons by Tukey HSD test are indicated for SMG gland, Lacrimal gland was not analysed due to undifferenciated tissue in LAC gland. "]
[]
[null,"A) Factorial ANOVA: dependent variable mean%GCT in SMG, factors: sex, subspecies and interaction between the two factors, statisticaly significant values (at p<0,05) are indicated in blod. "]
[]
[null,null,"Factorial ANOVA"]
[null,"rel SMG","SS","df","MS","F","p"]
[null,"Intercept",{"v":90036.53482414389,"d":"90036.53"},1,{"v":90036.53482414389,"d":"90036.53"},{"v":4451.923740955711,"d":"4451.924"},{"v":0,"d":"0.000000"}]
[null,"subspecies",{"v":1132.3077104985455,"d":"1132.31"},1,{"v":1132.3077104985455,"d":"1132.31"},{"v":55.98780082198274,"d":"55.988"},{"v":3.7735681690520551E-8,"d":"0.000000"}]
[null,"sex",{"v":3985.88328686115,"d":"3985.88"},1,{"v":3985.88328686115,"d":"3985.88"},{"v":197.08497742737805,"d":"197.085"},{"v":3.375077994860476e-14,"d":"0.000000"}]
[null,"subs. X sex",{"v":196.06838765212729,"d":"196.07"},1,{"v":196.06838765212729,"d":"196.07"},{"v":9.694747932539759,"d":"9.695"},{"v":0.004232619427155915,"d":"0.004233"}]
[null,"error",{"v":566.277214473317,"d":"566.28"},28,{"v":20.224186231189893,"d":"20.22"}]
[]
[null,"B) Posthoc comparison - Tukey HSD test of mean % of GCT in SMG per interaction of factors subspecies and sex, statisticaly significant values (at p<0,05) are indicated in blod. "]
[]
[null,"Tukey HSD test;"]
[null," variable rel SMG "]
[null,"df = 28"]
[null,null,"Factors"]
[null,null,"subspecies","sex","1 (33,459)","2 (60,731)","3 (50,307)","4 (67,678)"]
[null,1,"Dom","F",null,{"v":1.6447537479591379E-4,"d":"0.000164"},{"v":1.645161591623534E-4,"d":"0.000165"},{"v":1.6447537479580276E-4,"d":"0.000164"}]
[null,2,"Dom","M",{"v":1.6447537479591379E-4,"d":"0.000164"},null,{"v":5.4475145364762945E-4,"d":"0.000545"},{"v":0.022106448731696426,"d":"0.022106"}]
[null,3,"Mus","F",{"v":1.645161591623534E-4,"d":"0.000165"},{"v":5.4475145364762945E-4,"d":"0.000545"},null,{"v":0.000164492779788028,"d":"0.000164"}]
[null,4,"Mus","M",{"v":1.6447537479580276E-4,"d":"0.000164"},{"v":0.022106448731696426,"d":"0.022106"},{"v":0.000164492779788028,"d":"0.000164"}]

{"sheet":"Table S7","index":6,"n_rows":45,"n_cols":8,"rows_kept":45}
[]
[null,"Protein concentration"]
[]
[null,"Table S7: Results of statistical tests of the difference in total protein content in saliva and teras (separately) in WDS mice by factors sex and subspecies, complete results of Factorial ANOVA and Post Hoc comparisons by Tukey HSD test are indicated separately for saliva and tears. "]
[]
[null,"A) Factorial ANOVA: dependent variable Total protein concentration in saliva (meaured by BCA), factors: sex, subspecies and interaction between the two factors, statisticaly significant values (at p<0,05) are indicated in blod. "]
[]
[null,null,"Factorial ANOVA"]
[null,"BCA (saliva)","SS","df","MS","F","p"]
[null,"Intercept",{"v":147.5464934587688,"d":"147.5465"},1,{"v":147.5464934587688,"d":"147.5465"},{"v":109.92219282634973,"d":"109.9222"},{"v":2.4238389073616418E-12,"d":"0.000000"}]
[null,"subspecies",{"v":0.22117345609130418,"d":"0.2212"},1,{"v":0.22117345609130418,"d":"0.2212"},{"v":0.1647743075326448,"d":"0.1648"},{"v":0.6872695329605283,"d":"0.687270"}]
[null,"sex",{"v":0.13177150890595918,"d":"0.1318"},1,{"v":0.13177150890595918,"d":"0.1318"},{"v":0.09816982343282571,"d":"0.0982"},{"v":0.7558966946351007,"d":"0.755897"}]
[null,"subs. X sex",{"v":1.6715527505320116,"d":"1.6716"},1,{"v":1.6715527505320116,"d":"1.6716"},{"v":1.2453074245016915,"d":"1.2453"},{"v":0.2720538710374756,"d":"0.272054"}]
[null,"error",{"v":46.97984217995878,"d":"46.9798"},35,{"v":1.3422812051416795,"d":"1.3423"}]
[]
[null,"B) Posthoc comparison - Tukey HSD test of total protein concentration in saliva per interaction of factors subspecies and sex, statisticaly significant values (at p<0,05) are indicated in blod. "]
[]
[null,"Tukey HSD test;"]
[null," variable BCA (saliva)"]
[null,"df = 35"]
[null,null,"Factors"]
[null,null,"subspecies","sex","1 (2,1715)","2 (1,8734)","3 (1,6063)","4 (2,1371)"]
[null,1,"Dom","F",null,{"v":0.9432423896560216,"d":"0.943242"},{"v":0.6973799828069132,"d":"0.697380"},{"v":0.9999048322292341,"d":"0.999905"}]
[null,2,"Dom","M",{"v":0.9432423896560216,"d":"0.943242"},null,{"v":0.9581705067790862,"d":"0.958171"},{"v":0.9596585817735863,"d":"0.959659"}]
[null,3,"Mus","F",{"v":0.6973799828069132,"d":"0.697380"},{"v":0.9581705067790862,"d":"0.958171"},null,{"v":0.7363890489141902,"d":"0.736389"}]
[null,4,"Mus","M",{"v":0.9999048322292341,"d":"0.999905"},{"v":0.9596585817735863,"d":"0.959659"},{"v":0.7363890489141902,"d":"0.736389"}]
[null,"B) Factorial ANOVA: dependent variable Total protein concentration in tears (meaured by BCA), factors: sex, subspecies and interaction between the two factors, statisticaly significant values (at p<0,05) are indicated in blod. "]
[null,null,"Factorial ANOVA"]
[null,"BCA (tears)","SS","df","MS","F","p"]
[null,"Intercept",{"v":80.63489751207429,"d":"80.63490"},1,{"v":80.63489751207429,"d":"80.63490"},{"v":231.60723091125158,"d":"231.6072"},{"v":0,"d":"0.000000"}]
[null,"subspecies",{"v":5.767258296180759,"d":"5.76726"},1,{"v":5.767258296180759,"d":"5.76726"},{"v":16.565268452512814,"d":"16.5653"},{"v":1.9230608186837639E-4,"d":"0.000192"}]
[null,"sex",{"v":0.4224302355830153,"d":"0.42243"},1,{"v":0.4224302355830153,"d":"0.42243"},{"v":1.2133443476122674,"d":"1.2133"},{"v":0.2766600918567519,"d":"0.276660"}]
[null,"subs. X sex",{"v":0.022030335807547555,"d":"0.02203"},1,{"v":0.022030335807547555,"d":"0.02203"},{"v":0.06327762829570222,"d":"0.0633"},{"v":0.8025597608745165,"d":"0.802560"}]
[null,"error",{"v":15.318759593869435,"d":"15.31876"},44,{"v":0.34815362713339626,"d":"0.34815"}]
[]
[null,"D) Posthoc comparison - Tukey HSD test of total protein concentration in tears per interaction of factors subspecies and sex, statisticaly significant values (at p<0,05) are indicated in blod. "]
[null,"Tukey HSD test;"]
[null," variable BCA tears"]
[null,"df = 44"]
[null,null,"Factors"]
[null,null,"subspecies","sex","1 (1,5703)","2 (1,7151)","3 (0,8342)","4 (1,0647)"]
[null,1,"Dom","F",null,{"v":0.9312781712099824,"d":"0.931278"},{"v":0.019296543299002766,"d":"0.019297"},{"v":0.1694422327083237,"d":"0.169442"}]
[null,2,"Dom","M",{"v":0.9312781712099824,"d":"0.931278"},null,{"v":3.7737003711286743E-3,"d":"0.003774"},{"v":0.046674925359172614,"d":"0.046675"}]
[null,3,"Mus","F",{"v":0.019296543299002766,"d":"0.019297"},{"v":3.7737003711286743E-3,"d":"0.003774"},null,{"v":0.774466635781651,"d":"0.774467"}]
[null,4,"Mus","M",{"v":0.1694422327083237,"d":"0.169442"},{"v":0.046674925359172614,"d":"0.046675"},{"v":0.774466635781651,"d":"0.774467"}]

{"sheet":"Table S8","index":7,"n_rows":8,"n_cols":15,"rows_kept":8,"merged":[[2,0,2,1],[2,2,2,3],[2,4,2,10],[2,11,2,14],[3,1,3,2],[3,10,3,11],[4,1,4,2],[4,10,4,11],[5,1,5,2],[5,10,5,11],[6,1,6,2],[6,10,6,11],[7,1,7,2],[7,10,7,11]]}
["Table S8: Tear based discrimination: Results of coefficients of preference of the signal (tears or normal saline) in discrimination/preference test in Y-maze (t-test; H0: μ = 0), mean, SD and SE values are indicated separately per strain (subspecies) and sex. Statistically significant values at the significance level of 0.05 are in bold."]
[]
[{"$nulls":4},"Rsignal; t-test; H0: μ = 0",{"$nulls":6},"First choice of signal"]
["Subspecies","Sex",null,"Mean","SD","N","SE","t-value","df","p","Tears",null,"Saline","X2","p"]
["domesticus","F",null,"0.383","0.381",10,"0.121","3.174",9,"0.011",7,null,3,{"v":1.6,"d":"1.600"},"0.206"]
["domesticus","M",null,"0.359","0.483",10,"0.153","2.350",9,"0.043",9,null,1,{"v":6.4,"d":"6.400"},"0.011"]
["musculus","F",null,"0.134","0.375",11,"0.113","1.185",10,"0.264",5,null,6,"0.091","0.763"]
["musculus","M",null,"0.126","0.572",11,"0.172","0.731",10,"0.482",5,null,6,"0.091","0.763"]

{"sheet":"Fig S1","index":8,"n_rows":3,"n_cols":10,"rows_kept":3}
["Figue S1: SSD in mice from WDS strains of both subspecies, variability in realtive gland size between strains and sexes within each subspecies separately for SMG gland (A) and LAC gland (B); box plots indicate mean values +/- SE and  whiskers are +/- 95%CI. High interstrain variablity is due to differences in N of animals scored within each strain (for details see Table S2)."]
["D/M=domesticus/musculus subspecies, M/F=male/female, DDO = name of the strain"]
["A)",{"$nulls":8},"B)"]

{"sheet":"Fig S2 SSD histo","index":9,"n_rows":4,"n_cols":3,"rows_kept":4}
[]
[]
[]
[null,null,"Figure S2  Histological sections of the submandibular gland in wild-derived strains of both subspecies: BUSNA, MBK (M. m. musculus) and STRA, DROS (M. m. domesticus) and classical lab strain BL6 as a control. A) Mean values of the relative amount of GCT (+/-  95 CI) are indicated separately for males and females. B) Examples of the histological sections with a graphic representation of GCT analyses in the FiJi Image (inserts): GCTs are lighter, surrounded by darker acinar tissues"]