Table S1:  Proximate and ultimate analysis of collected waste coal sample

Test	 Method	Waste coal	
Proximate Analysis	
Ash  content (%) by mass	IS: 1350 (P-1) 1984	42.52	
Moisture content (%) by mass	IS: 1350 (P-1) 1984	5.99	
Volatile Matter (%)	IS: 1350 (P-1) 1984	14.85	
Fixed Carbon (%)	IS: 1350 (P-1) 1984	42.14	
Gross calorific value, k.cals/kg	IS: 1350 (P-2) 1975	4092	
Ultimate analysis	
Carbon, %	IS: 1350 (P-4/sec 1) 1974	44.71	
Hydrogen, %	IS: 1350 (P-4/sec 1) 1974	2.55	
Nitrogen, %	IS: 1350 (P-4/sec 2) 1999	0.04	
Sulphur, %	IS: 1350 (P-3) 1969	0.28	
Oxygen, %	IS: 1350 (P-4/sec 1) 1974	9.41	

B

A

Fig. S1: A and B, illustrated the percent of gas generation by reactivated consortia with variable nitrogen sources (Urea and CLS). Data recoded after 15-20th days of incubation.

Table S2: Chemical moiety present in coal containing cultured bottles, identified through GCMS

S.No	RT	Chemical Name	Molecular Formula	Molecular weight (g/mol)	
1	13.85	1-Dodecene	C12H24	168.31	
2	22.49	2-Tetradecene	C14H28	196.37	
3	22.83	n-Tetradecene 	C12H30	198.39	
4	27.39	2,4-Di-tert-butyl phenol	C14H22O	206.32	
5	30.51	1-Hexadecene	C16H33	224.42	
6	37.74	5-Octadecene	C18H36	252.5	
7	40.64	Cyclohexadecane	C16H32	224.42	
8	44.32	E-15 Heptadecenal	C17H32O	252.4	
9	44.52	Eicosane	C20H42	282.54	
10	47.05	1-Nonadecanol	C19H40O	284.5	
11	50.33	1-Tetracosanol	C24H50O	354.65	
12	61.00	Cyclotetracosane	C24H28	336.6	
13	65.60	Triacontyl acetate	C32H64O	480.8	

Table S3: Group mean clinical signs data of male and female mice.
Group	Days	
Control	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	
Male	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	
Female	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	
Group	Days	
Test	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	
Male	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	
Female	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	N	

•	Note : N= Normal/No signs of toxicity
Table S4: Group mean haematology data of male (M) and female (F)
Group		Hb %	PCV %	RBC 
(million/cmm)	WBC 
(thousand/cmm)	BUN 
(mg%)	Albumin
 (gm %)	Glucose
 (mg %)	TP 
(gm %)	
Control (M)	Mean	9.67	30.57	6.54	5.23	41.34	3.2	117.3	5.6	
	± SD	1	2.04	0.53	0.84	6.13	0.2	36.09	0.61	
Test (M)	Mean	9.47	30.93	6.88	4.53	44.12	2.87	124.67	5.57	
	± SD	1.25	4.76	0.94	1.8	8.35	0.06	16.5	8.35	
Control (F)	Mean	8.57	26.47	5.75	3.07	43.3	3.17	118.67	5.33	
	± SD	3.35	10.36	1.98	0.12	1.87	0.06	6.43	0.47	
Test (F)	Mean	10.73	33.27	7.15	6.83	34.46	3.23	126.67	5.87	
	± SD	0.06	0.21	0.29	2.96	4.9	0.32	10.5	0.32	

•	Note : M= Male, F= Female, TP= Total Protein and ± SD= Standard Division
Table S5: Group mean body weight data of male (M) and female (F)

Group & Dose	Day 0 	Day 7	Difference (7-0)	Day 14	Difference (14-0)	Day 21	Difference (21-0)	
Control (M) 00 	Mean	19.67	23.17	28.5	34.33	19.67	23.17	28.5	
	± SD	0.76	0.29	0.5	1.04	0.76	0.29	0.5	
Test (M)
1ml containing 1.0X108 CFU	Mean	19.57	23.5	29.67	35	19.57	23.5	29.67	
	± SD	0.9	0.5	0.29	0.5	0.9	0.5	0.29	
Control (F) 00	Mean	19.77	23.83	29.67	35.5	19.77	23.83	29.67	
	± SD	1.08	0.76	0.29	0.5	1.08	0.76	0.29	
Test (F) 1ml containing 1.0X108 CFU	Mean	19.67	23.33	28.17	34	19.67	23.33	28.17	
	± SD	1.61	1.04	1.04	1.32	1.61	1.04	1.04	

Table S6:  Group mean mortality data
Group	Dose	No. of mice	Terminally sacrificed	Found dead 	
1	Control (M)	3	3	0	
2	1ml containing 1.0X108 CFU (M)	3	3	0	
Total	6	6	0	
3	Control (F)	3	3	0	
4	1ml containing 1.0X108 CFU (F)	3	3	0	
Total	6	6	0	

•	Note: M= male and F= Female