Supplemental FIGURES

Supplemental Figure S1: Pairwise comparison of Cortisol, Adrenalin/Noradrenaline, and cfnDNA in plasma before and after psychosocial and physical stress

The values of all 20 participants are represented by differently colored curves for all time points before and after psychosocial and physical stress. The black curve represents the means of all participants at each experimental time point, including standard deviations. sEVs given in objects/mL; Adrenaline/Noradrenaline; Plasma cortisol in nmol/L; cfDNA in copies/mL. Detailed information on the statistical analysis can be found elsewhere.

Supplemental Figure S2: Plasma levels and time course of the remaining 15 sEVs

Panels A–O descriptively display the abundance of 15 plasma sEV markers, measured in [objects/mL], over the course of the experiment. No statistical post hoc tests were conducted for these markers, as repeated-measures ANOVA revealed no significant time × stress type interactions. Colored lines indicate intra-individual trajectories of sEV levels, while black lines represent group means. Error bars show the standard error of the mean (SEM). Note that although the y-axis is consistent within each facet plot, it varies across sEVs due to the generally heterogeneous plasma concentrations.