{"text_format":"3","workbook":"xls","n_sheets":1,"sheets":["Лист1"],"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Лист1","index":0,"n_rows":18,"n_cols":9,"rows_kept":18,"merged":[[0,4,0,5],[0,6,0,7],[2,0,5,0],[2,1,5,1],[2,2,5,2],[2,3,3,3],[2,8,5,8],[4,3,5,3],[7,0,10,0],[7,1,10,1],[7,2,10,2],[7,8,10,8],[12,0,17,0],[12,1,17,1],[12,2,17,2],[12,3,13,3],[12,8,17,8]],"flags":["formulas_cached_only"]}
[null,"Vector","Stage","Substage","Forward primer",null,"Reverse primer",null,"Cloning method"]
[1,"pFGG-Lic","Интеграция гена лихеназы LicBM3 в pFGG","Амплификация гена LicBM3,  рестрикция ","Lic_F","ATGAGCTCATGGTGGTAAATACGCCTTTTG","Lic_R","ATCCCGGGACCGTTAGGATAGTATTTT","SacI/SmaI"]
[2,"pFGG-Lic(STL)","Клонирование интрона STL ген лихеназы","Линеаризация pFGG-Lic","PF_lin_F1","ATACGGTTATTATGAAGTAAGAATG","PF_lin_R1","GTACGATATTCACCGCTTTTATAC","SLiC"]
[{"$nulls":4},"PF_lin_F2","AAAATCATTTTTCGGATACGGTTATTATG","PF_lin_R2","CTTTTATACGGATATGAACCGCC"]
[{"$nulls":3},"Вставка интрона","STL_F1","GTAAGTTTCTGCTTCTACCTTTG","STL_R1","CCGAAAAATGATTTTCTGCACATCAAC"]
[{"$nulls":4},"STL_F2","CGGTGAATATCGTACGTAAGTTTCTGC","STL_R2","CTGCACATCAACAAATTTTGGTC"]
[3,"pGLR","Вставка кассеты p35Sen-Lic(STL)-T35S в вектор pVIG-T","Рестрикция","-","-",null,"-","SpeI/XhoI"]
["4.1","-","Синтез кассеты pACT-uidA(CBCI)-Tnos","Амплификация pACT","PACT_F","ATCTCGAGGGTACCACGCGTTTCGAC","PACT_R","TGGGATCCTTTGTTGAAATTGTGCCATGTTGTTGTTG","PCA"]
[{"$nulls":3},"Амплификация uidA","UID_F","GAGTCCCGCTTATCACACGTGGTG","UID_R","CAAAGGATCCCAATGGTAGATCTGAGGG"]
[{"$nulls":3},"Амплификация Tnos","TNOS_F","TAAGCGGGACTCTGGATCTAGAGTCAAGCAGATCGTTC","TNOS_R","AACCTCGAGTGCAGGTCGATCTAGTAACATAGATG"]
[{"$nulls":3},"Амплификация кассеты pACT-uidA(CBCI)-Tnos","PACT_F","ATCTCGAGGGTACCACGCGTTTCGAC","TNOS_R","AACCTCGAGTGCAGGTCGATCTAGTAACATAGATG"]
["4.2","pLAUMe","Интеграция кассеты pACT-uidA(CBCI)-Tnos в pGLR","Рестрикция","-","-","-","-","XhoI"]
["5.1-5.8","pLAUMe-ENC","Вставка специфического трансляционного энхансера","Линеаризация pLAUMe","PLAUME_F1","ATGGTGGTAAATACGCCTTTTG","PLAUME_R1","ATGAACTTCCTTATATAGAGGAAGGG","SLiC"]
[{"$nulls":4},"PLAUME_F2","CCTTTTGTTGCAGTGTTTTCGAAC","PLAUME_R2","TCCTCTCCAAATGAAATGAACTTCC"]
[{"$nulls":3},"Синтетический энхансер","AT30_F","TTCATTTGGAGAGGACTCTAATCACCAGGAGTAAAA","AT30_R","CGTATTTACCACCATTTTTACTCCTGGTGATTAGAG"]
[{"$nulls":4},"AT65_F","TTCATTTGGAGAGGAGAGAGAAGAAAGAAGAAGACG","AT65_R","CGTATTTACCACCATCGTCTTCTTCTTTCTTCTCTC"]
[{"$nulls":4},"SynM_F","TTCATTTGGAGAGGAACACGCTGGAATTCTAGTATACTTTTCCATG","SynM_R","CGTATTTACCACCATCATGGAAAAGTATACTAGAATTCCAGCGTGT"]
[{"$nulls":4},"GGR_F","TTCATTTGGAGAGGAACAAACTCAAAACACAGAGAACAGAGAGAGAGAGAGAGACTCCATTGTTGAAGTGTGTTC","GGR_R","CGTATTTACCACCATTGTTGTTTGAGGAGGAGTTCAGTGAACACACTTCAACAATGGAGTCTCTCTCTCTC"]