{"text_format":"3","workbook":"xls","n_sheets":10,"sheets":["Table S1","Table S2","Table S3","Table S4","Table S5","Table S6","Table S7","Table S8","Fig S1","Fig. S2"],"not_rendered":{"images":2,"external_links":2,"defined_names":11},"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Table S1","index":0,"n_rows":86,"n_cols":5,"rows_kept":86,"merged":[[0,0,0,4]],"flags":["formulas_cached_only"]}
["Table S1. The details of gladiolus genotypes involved in the present study "]
["Genotype ID","Genotype name","Origin","Parentage","Place of collection"]
[1,"Anglia","Exotic","Eloise × Rosemarie Pfitzer","ICAR-IARI, New Delhi"]
[2,"Amethyst","Exotic","Lavender Puff × Tropic Sea","CSIR-NBRI, Lucknow"]
[3,"Pusa Gunjan","Indian","White Oak × Oscar","ICAR-IARI, New Delhi"]
[4,"Punjab Glad 1","Indian","Happy End × True Yellow","PAU, Ludhiana"]
[5,"Aldebaran","Exotic","Unknown","CSIR-NBRI, Lucknow"]
[6,"Fire Flame","Exotic","(Big Top × Crystal × Red Ruffle) × (Vagabond Prince × New Era × Rich)","ICAR-IIHR, Bengaluru"]
[7,"Tiger Flame","Exotic","Sunskit × Unknown","CSIR-NBRI, Lucknow"]
[8,"Kalima","Indian","Open pollinated seedling of Sylvia","CSIR-NBRI"]
[9,"Neelima","Indian","Snow Princess × Tropic Sea","CSIR-NBRI, Lucknow"]
[10,"GS-2","Indian","Unknown","ICAR-IARI, New Delhi"]
[11,"Praha","Indian","Unknown","CSIR-NBRI, Lucknow"]
[12,"Anjali","Indian","Sancerre × Rose Spire","ICAR-IARI, New Delhi"]
[13,"Malaviya Shatabdi","Indian","Induced mutant of Punjab Dawn","BHU, Varanasi, UP"]
[14,"Roshni","Indian","Friendship Pink × Red Beauty","CSIR-NBRI, Lucknow"]
[15,"Yellow Stone","Exotic","Unknown","PAU, Ludhiana"]
[16,"Dhanvantari","Indian","Jr Prom × Lucky Star","ICAR-IARI, New Delhi"]
[17,"Punjab Morning","Indian","Sancerre × White Prosperity","PAU, Ludhiana"]
[18,"Rose Supreme","Exotic","Unknown","PAU, Ludhiana"]
[19,"Punjab Glance","Indian","Happy End × Yellow Stone","PAU, Ludhiana"]
[20,"Sancerre","Exotic","Unknown","ICAR-IARI, New Delhi"]
[21,"Pusa Shagun","Indian","White Oak × Oscar","ICAR-IARI, New Delhi"]
[22,"Punjab Glad 2","Indian","Unknown","PAU, Ludhiana"]
[23,"Pusa Shabnam","Indian","Lucky Shamrock × Green Lilac Open","ICAR-IARI, New Delhi"]
[24,"Punjab Dawn","Indian","Suchitra × Melody","PAU, Ludhiana"]
[25,"Arka Tilak","Indian","Watermelon Pink × Lady John","ICAR-IIHR, Bengaluru"]
[26,"Victor","Exotic","Unknown","ICAR-IARI, New Delhi"]
[27,"Pusa Lohit","Indian","Creamy Green × American Beauty","ICAR-IARI, New Delhi"]
[28,"Malaviya Kiran","Indian","Induced mutant of Pusa Kiran","BHU, Varanasi, UP"]
[29,"Pusa Manmohak","Indian","Mayur × Hunting Song","ICAR-IARI, New Delhi"]
[30,"Pusa Sindhuri","Indian","Open pollinated seedling of Little Fawn","ICAR-IARI, New Delhi"]
[31,"Arka Nazrana","Indian","Black Jack × Friendship","ICAR-IIHR, Bengaluru"]
[32,"Nova Lux","Exotic","Unknown","CSIR-NBRI, Lucknow"]
[33,"Euro Vision","Exotic","Unknown","CSIR-NBRI, Lucknow"]
[34,"Lady Jane","Exotic","(Golden Measure × Belinda) × (Maid of Orleans × Picardy)","ICAR-IIHR, Bengaluru"]
[35,"Jacksonville Gold","Exotic","Unknown","PAU, Ludhiana"]
[36,"Punjab Lemon Delight","Indian","Jacksonville Gold × White Prosperity","PAU, Ludhiana"]
[37,"Arka Aayush","Indian","Gold Medal - 412 × Poonam","ICAR-IIHR, Bengaluru"]
[38,"Pusa Sringarika","Indian","Mayur × Heady Wine","ICAR-IARI, New Delhi"]
[39,"Pusa Red Valentine","Indian","Open pollinated selection from Regency","ICAR-IARI, New Delhi"]
[40,"Neel Rekha","Indian","Unknown","ICAR-IARI, New Delhi"]
[41,"Yellow Star","Exotic","Parade × Moon Mirage","BHU, Varanasi"]
[42,"Creamy Green","Exotic","Unknown","ICAR-IARI, New Delhi"]
[43,"Friendship","Exotic","(Maid of Oleans × Picardy) × (Gloaming × Seedling)","ICAR-IARI, New Delhi"]
[44,"Pink Lady","Exotic","(Pink Prospector × Pink Plume) × (Pale Moon × Galah)","ICAR-IARI, New Delhi"]
[45,"Arka Sapna","Indian","Green Woodpecker × Friendship","ICAR-IIHR, Bengaluru"]
[46,"Arka Kesar","Indian","Vink's Glroy × Sagar","ICAR-IIHR, Bengaluru"]
[47,"Pusa Suhagin","Indian","Open pollinated seedlings of Sylvia","ICAR-IARI, New Delhi"]
[48,"Pusa Urmi","Indian","Berlew × Heady Wine","ICAR-IARI, New Delhi"]
[49,"Pusa Chirag","Indian","Cygnet × Little Fawn","ICAR-IARI, New Delhi"]
[50,"Nicole","Exotic","Sister of Jessica × Promise","CSIR-NBRI, Lucknow"]
[51,"Pusa Archana","Indian","Creamy Green × American Beauty","ICAR-IARI, New Delhi"]
[52,"Arka Darshan","Indian","Watermelon Pink × Shirley","ICAR-IARI, New Delhi"]
[53,"Plumtart","Exotic","Last Rose × Jack of Spades","ICAR-IARI, New Delhi"]
[54,"Pusa Sarang","Indian","Open pollinated seedling of White Oak","ICAR-IARI, New Delhi"]
[55,"Fidelio","Exotic","Unknown","CSIR-NBRI, Lucknow"]
[56,"Urmil","Indian","Tinker Belle × Break O’Dawn","ICAR-IARI, New Delhi"]
[57,"Lucky Shamrock","Exotic","(Lemon Lime × Green Ice) (Green Ice × Green Ruffles)","ICAR-IARI, New Delhi"]
[58,"Delhi Callianthus","Indian","Unknown","ICAR-IARI, New Delhi"]
[59,"Pusa Urvashi","Indian","Open pollinated seedlings of Red Soft Glow","ICAR-IARI, New Delhi"]
[60,"Pusa Chandni","Indian","Green Woodpecker × White Butterfly","ICAR-IARI, New Delhi"]
[61,"Suchitra","Indian","Sylvia Seedlings × Jo Wagenaar","ICAR-IARI, New Delhi"]
[62,"Surya Kiran","Indian","Unknown","ICAR-IARI, New Delhi"]
[63,"Arka Amar","Indian","Watermelon Pink × Arka Aarti","ICAR-IIHR, Bengaluru"]
[64,"True Love","Exotic","Unknown","CSIR-NBRI, Lucknow"]
[65,"Peter Pear's","Exotic","Salome × Maolete","ICAR-IARI, New Delhi"]
[66,"Pusa Shweta","Indian","Wind Song × Pink Frost","ICAR-IARI, New Delhi"]
[67,"Arka Poonam","Indian","Geliber Herald × R.N. 121","ICAR-IIHR, Bengaluru"]
[68,"Pusa Srijana","Indian","Berlew × Heady Wine","ICAR-IARI, New Delhi"]
[69,"Pusa Gulal","Indian","Snow Princess × Viola","ICAR-IARI, New Delhi"]
[70,"Vicki Lin","Exotic","Unknown","ICAR-IARI, New Delhi"]
[71,"African Star","Exotic","Unknown","ICAR-IARI, New Delhi"]
[72,"Arka Gold","Indian","Green Bay × Gold Medal - 412","ICAR-IIHR, Bengaluru"]
[73,"Pusa Kiran","Indian","Open pollinated seedlings of Ave","ICAR-IARI, New Delhi"]
[74,"Arka Aarti","Indian","Shirley × Melody","ICAR-IIHR, Bengaluru"]
[75,"Arka Naveen","Indian","74-39-1 × Tropic Sea","ICAR-IIHR, Bengaluru"]
[76,"Apple Blossom","Exotic","Beauty’s Blush × Rose Charm","ICAR-IARI, New Delhi"]
[77,"Punjab Pink Elegance","Indian","Suchitra × White Prosperity","PAU, Ludhiana"]
[78,"Vink's Glory","Exotic","Unknown","CSIR-NBRI, Lucknow"]
[79,"Pusa Bindiya","Indian","Open pollinated seedling of Ratna’s Butterfly","ICAR-IARI, New Delhi"]
[80,"Pusa Mohini","Indian","Ave × Christian Jane","ICAR-IARI, New Delhi"]
[81,"Summer Sunshine","Exotic","Aurora × Sunkist","ICAR-IARI, New Delhi"]
[82,"Algarve","Exotic","Priscilla × Antonio","ICAR-IARI, New Delhi"]
[83,"Malaviya Kundan","Indian","Induced mutant of Tiger Flame","BHU, Varanasi, UP"]
[84,"C.P.G","Indian","Unknown","PAU, Ludhiana"]

{"sheet":"Table S2","index":1,"n_rows":75,"n_cols":8,"rows_kept":75,"merged":[[0,0,0,7],[2,0,2,7],[15,0,15,7],[31,0,31,7],[57,0,57,7],[65,0,65,7],[68,0,68,7],[71,0,71,7],[74,0,74,7]],"flags":["formulas_cached_only"]}
["Table S2. SSR markers selected for the study, primer sequence, amplification type and annealing temperature  "]
["Sl. No","Marker Name","Forward Primer (5'-3')","Reverse Primer (5'-3')","A/NA","Tm (°C)","Allele size (bp) ","Nature of allele"]
["Chloroplast derived SSRs  (cPSSRs) of Gladiolus "]
[1,"G1SSR","CCTTGGTATGGAAACCTGCTAA","ATAGATCCACAATTTCTCCCCA","A",60,320,"Monomorphic"]
[2,"G2SSR","GGTATGGAAACCTGCTAAGTGG","TAGATCCACAATTTCTCCCCAT","A",60,310,"Monomorphic"]
[3,"G3SSR","GGAAGCTGTTCTAACGAATGGA","TATTGGGGATAGAGGGACTTGA","A",60,370,"Monomorphic"]
[4,"G4SSR","GGAGACTGACTAGGGCAAAAGA","AACTCCTTGACGCATTACGACT","A",60,380,"Monomorphic"]
[5,"G5SSR","GCTCACAACAATAATCCTTCCC","CAATGAACTCAGCAATACCAGC","A",60,"270-300","Polymorphic"]
[6,"G6SSR","GAAGAAGCGTGAGAAATCCATA","GGACCAACCGCAATAAATAA","A",60,350,"Monomorphic"]
[7,"G7SSR","GTGTCTTCGGTGCTTTTCTCTT","CAGCGATAACCTAGAACGAACA","A",60,"260-320","Polymorphic"]
[8,"G8SSR","TCTATGTCAGTGCTCTACCGGA","GAAGCAAACGAGTCTGTGGAC","A",60,"280-300","Polymorphic"]
[9,"G9SSR","TATAGAGGAATGCGTGTCCGAT","TACTGCATGACGAGGAAATCAC","A",60,"400-420","Polymorphic"]
[10,"G10SSR","TGCCACTCCAGCATAACTTCTA","ACTCCTTTTCCTCCCATTCTTC","A",60,"310-330","Polymorphic"]
[11,"G11SSR","AAAGTCCCTCCTCTCCTCTGAT","GAGCTTGTTACTGAACGGAACC","A",60,"480-510","Polymorphic"]
[12,"G12SSR","GGCATCCTTCCTCTCCGT","CGGCCTTGGGTGTAGAAGTAG","A",60,"200-240","Polymorphic"]
["Genomic SSRs from Gladiolus palustris"]
[13,"GP1","TTCTGCAAAGCTAACAGGCG","CCACTGCCATTATCGGTCGA","NA","-","NA","-"]
[14,"GP2","TTGTTACTGGTGCGGACTCC","CAGGTCCGATTGCTTGAGGA","A",58,"210-270","Polymorphic"]
[15,"GP3","TTTGGGACGGGTATGCGTAG","TTCGCTTTCGTGGAAGTCCA","A",55,"250-300","Monomorphic"]
[16,"GP4","ATGCCTTTGTCCTCTCACCT","TTTGTCCCTAATTGGAACACGTC","A","(59°C-54 °C, -0.5 °C per cycle)","180-310","Polymorphic"]
[17,"GP5","CGGGAATAGCAACTACCCGG","GTTGCCCTTGCGGTTGATC","A",55,110,"Monomorphic"]
[18,"GP6","ATTGCCAAGGAGGTACAGGC","GCAAACCCACTCTGCTTCCT","A",54,110,"Monomorphic"]
[19,"GP7","CCAAGTAAGTGATGGCGGC","GGGTCTAGAGAAGGCTTGGG","A",56,"190-210","Polymorphic"]
[20,"GP8","GTGGGTTCCAAGCTTGCATG","TCATTGGCCGCGACAGAG","A",56,"≈180-200","Not scorable"]
[21,"GP9","TGAAACCCTACACGTAACCCT","ATGTAGGGTGATGGTGGCTG","NA","-","NA","-"]
[22,"GP10","ACATTCCTATTTGGACGGTCCA","AGTTCCCTAGTCATCTATGGCT","NA","-","NA","-"]
[23,"GP11","ACGTGAGTGATCAATTAAAGGGC","CAACAGCACTGTGAGCACC","NA","-","NA","-"]
[24,"GP12","ATCCCTTTCCTAATTCCTTCCC","TGATGAAGAGGATTCCGACGAC","A",54,180,"Monomorphic"]
[25,"GP13","AAACCCTCACTTCGGAGATCA","TAAAGTCAGTCAGCTGTAACACTG","A",54,"280-300","Polymorphic"]
[26,"GP14","TCGGAAGGTAACAGAGGGAAC","GTCGAAACAGAGGAGATCGGG","NA","-","NA","-"]
[27,"GP15","GGGTCATCGCCTGTCATGAA","TCGTATCGGCTTGTTGGCTG","A",54,"190-220","Polymorphic"]
["EST SSRs from Iris spp."]
[28,"IM9","TGTGTAAGAAGAAGTGCAGTTTGA","TGGAGCCTCTGGTACTTGCT","NA","-","NA","-"]
[29,"IM27","ACTGCTCTTGCATTTTACTTC","ATTACGAATTGCTTGCGTGAG","A",52,"220-260","Monomorphic"]
[30,"IM31","AAGCAAAAGGTTTTCCATTCC","GTTTCTTGTCGAGGAACATGC","A",52,"300-420","Polymorphic"]
[31,"IM37","AGAGAGGTGAGGTGAGGGTTT","CTTCCATCGTGTCCGAGTATG","NA","-","-","-"]
[32,"IM38","TCCTCCCTCACTCTCTTCCAT","AAAGCTCAGGCTACGCACAT","NA","-","-","-"]
[33,"IM39","CCCTAGCAAACATCTCTTCCA","TGTTATCAGCAAGCAGTCCAG","A","(59-54 °C, -0.5 °C per cycle)","380-500","Polymorphic"]
[34,"IM46","GAAGTTGGGAAACGACCATTA","GGGTTATGGTTAGGGTTAGGG","NA","-"]
[35,"IM86","GGGTTTGTATTGTTTGTTGGAGA","GGGTGATGTGGTCCTTGTAGA","A",60,"200-220","Polymorphic"]
[36,"IM108","TCTCCTCCTGTCCGTCTATCC","AGTCGTCCAAATCTCCGAACT","A","(50°C- 45°C,  -0.5 °C per cycle)","320-390","Polymorphic"]
[37,"IM112","ACGAGAAGAAGGAAGCCAAAC","TCTCAACAAACATAACGGCAAA","A","(55°C– 50 °C,  -0.5 °C per cycle)","200-300","Not scorable"]
[38,"IM113","GCAAGCATACGTTCCACAAGT","GCGACACATCATACACAAACG","NA","-","-","-"]
[39,"IM114","CAAGTGTTACAAAGTGAGAAGAAGGA","ATTCTCCAGAAGTCCGACACA","A",60,620,"Monomorphic"]
[40,"IM123","GTTTCATGGAGGAGTTGCAGT","TATCTTGCGGGTCTTCTTGG","A","(50°C- 45°C,  -0.5°C per cycle)",320,"Monomorphic"]
[41,"IM151","TTGTTCTCAGTCCAGCTCCAG","TCTTCTCCTCCACCTCCTGTT","A",52,"580-850","Monomorphic"]
[42,"IM221","CCTTTCCGTTCCATTCTAGAGAC","CCAAACTCTCTCCCTTGACCT","NA","-","-","-"]
[43,"IM223","AATCATCACCCACCTCCATTT","TCATCCACAATTAGGGTTCCA","NA","-","-","-"]
[44,"IM224","AGAGAAGAGAGCATGGCGATA","GCGAGAAGTGGCATAAAGAGA","A",46,"200-280","Polymorphic"]
[45,"IM234","ACCAAATGATGGCTGCTGTTA","CAAGAGTTCCGTTGAGGTGAG","NA","-","-","-"]
[46,"IM242","CCATCGTCTATCTCCACTCCA","GGACTGCTCCGATAGCTTCTT","NA","-","-","-"]
[47,"IM226","GTTGTCTACCATGTCCAACTTCC","TATTGTGGTGTCCCATGCTTT","NA","-","-","-"]
[48,"IM309","TGAAATCCAAAGCCAAACAAG","GCTCGTACTTCTCCATTGCTG","NA","-","-","-"]
[49,"IM408","AAGAAACCATAATATACAAATTGAAG","GCTCGTTGAGGAAGATGACAG","NA","-","-","-"]
[50,"IM400","CCCACTTTCTCATTCCTCTCC","CTGCAGAAGAAGAGCTCGACA","A",52,550,"Monomorphic"]
[51,"IM158","CCAACCTACCAATCCCTCTTC","GATTAGCCAGCAGTCCTCCTT","NA","-","-","-"]
[52,"IM60","TACTTGGAGTTGTGGCTGGTT","AGATTGGAGAAAGGGAAGCAC","A",54,200,"Monomorphic"]
["Genomic SSRs from Crocus sativus"]
[53,"Cs2","ATACGGTAACATCAGGAAG","AGTAATCCACGCGTCAAGGT","A",52,550,"Monomorphic"]
[54,"Cs8","GTGTAATGAATGGGATATATGGC","CCTTCCAACTGTGAAATAATTCC","NA","-","-","-"]
[55,"Cs10","GGATGTACTTAGGTTGTG","GGAAACCCTAACTAGGT","A",52,"≈ 450","Not scorable"]
[56,"Cs11","CCCAACTGACCTTCCAACTG","GTTGTTATGATGGTCTGGCC","A",52,"≈ 500","Not scorable"]
[57,"Cs20","CAATCTTTACATAGTGAGGC","GTATTCTGGTCAGTTCAGTG","NA","-","-","-"]
[58,"Cs21","TACCCTATAAAGAGTGGACA","GCTGCCTAGTAATGTGTAAG","A",54,"120-160","Monomorphic"]
[59,"Cs28","AACCACTGAGGAAGGAC","GGTAGAATACCTTATCGGTT","A",52,"≈150","Not scorable"]
["Genomic SSRs from Herbertia zebrina"]
[60,"HZ4","TAGGCCCCACCAACGTATAA","AACACGTCATCTTCGTCTTCC","NA","-","-","-"]
[61,"HZ5","TTGGGTTGTATCTCGTATCTGG","TGCCATGTACATCCCTTAAATC","NA","-","-","-"]
["Genomic SSRs from Sysirinchium micranthum"]
[62,"SM-A10","TATTTGCTGATTTCTTGTTTTA","TCTGTATGAATAGCCTCTTTTT","NA","-","-","-"]
[63,"SMB5","ATTATCAGCCAAAACAAAGAAGG","TAGCCGGTGCAATAGGTAAAG","NA","-","-","-"]
["Genomic SSRs from ITS regions (Intergenic spacers)"]
[64,"psbA F -trnH R","GTTATGCATGAACGTAATGCTC","CGCGCATGGTGGATTCACAAATC","A",58,650,"monomorphic"]
[65,"trnL F-trnH R","AAAATCGTGAGGGTTCAAGTC","GATTTGAACTGGTGACACGAG","A",58,260,"monomorphic"]
["Note: A – Amplified; NA – Not amplified; Tm -Annealing temperature"]

{"sheet":"Table S3","index":2,"n_rows":10,"n_cols":7,"rows_kept":10,"merged":[[0,0,0,6]],"flags":["formulas_cached_only"]}
["Table S3. Source of SSRs and their amplification pattern in gladiolus "]
["Sl. No","Species/genera ","Type of marker","No. of screened primers ","No. of amplified primers ","Amplification %","No. of Polymorphic markers"]
[1,"Gladiolus spp.","Chloroplast SSRs",12,12,{"v":100,"d":"100.00"},7]
[2,"Gladiolus palustris","Genomic SSRs",15,10,66.66,5]
[3,"Iris spp. ","EST derived SSRs",25,12,{"v":48,"d":"48.00"},5]
[4,"Crocus sativus","Genomic SSRs",7,5,71.42,0]
[5,"Herbertia zebrina ","Genomic SSRs",2,0,{"v":0,"d":"0.00"},0]
[6,"Sysirinchium micranthum ","Genomic SSRs",2,0,{"v":0,"d":"0.00"},0]
[7,"Chloroplast DNA regions ","Intergenic spacers",2,2,{"v":100,"d":"100.00"},0]
[null,"Total",null,65,41,63.07,17]

{"sheet":"Table S4","index":3,"n_rows":20,"n_cols":8,"rows_kept":20,"merged":[[0,0,0,7],[19,0,19,7]],"flags":["formulas_cached_only"]}
[" Table S4. The Ewens-Watterson Test for Neutrality  of SSR loci for overall gladiolus genotypes"]
["Locus","Obs. F","Min F","Max F","Mean*","SE*","L95*","U95*"]
["G5",0.36,0.25,0.96,{"v":0.6,"d":"0.60"},0.03,0.32,0.93]
["G7",0.78,0.25,0.96,0.59,0.03,0.32,0.93]
["G8",0.42,0.33,0.98,0.69,0.04,0.36,0.98]
["G9",0.89,{"v":0.5,"d":"0.50"},0.99,0.82,0.03,{"v":0.5,"d":"0.50"},0.99]
["G10",0.69,0.25,0.96,0.59,0.03,0.31,0.94]
["G11",{"v":0.4,"d":"0.40"},0.33,0.98,{"v":0.7,"d":"0.70"},0.03,0.39,0.98]
["G12",{"v":0.3,"d":"0.30"},0.25,0.96,{"v":0.6,"d":"0.60"},0.03,0.32,0.93]
["GP2",0.94,0.33,0.97,0.69,0.03,0.37,0.97]
["GP4",0.58,0.17,0.92,0.43,0.02,0.23,0.81]
["GP7",0.36,0.33,0.98,{"v":0.7,"d":"0.70"},0.03,0.38,0.96]
["GP13",0.37,0.33,0.98,0.69,0.03,0.38,0.98]
["GP15",0.36,0.25,0.96,0.59,0.03,0.32,0.94]
["IM31",0.31,0.25,0.96,0.58,0.03,0.32,0.93]
["IM86",0.86,{"v":0.5,"d":"0.50"},0.99,0.83,0.03,{"v":0.5,"d":"0.50"},0.99]
["IM108",0.62,{"v":0.5,"d":"0.50"},0.99,0.81,0.03,{"v":0.5,"d":"0.50"},0.99]
["IM39",0.84,0.33,0.97,0.69,0.03,0.38,0.97]
["IM224",0.68,0.25,0.96,0.59,0.03,0.32,0.93]
["*These statistics were computed upon 1000 permutations for each loci. Observed F value (sum of square of allelic frequency) highlighted in bold lied\noutside the lower and upper limit of 95% confidence interval. "]

{"sheet":"Table S5","index":4,"n_rows":87,"n_cols":9,"rows_kept":87,"merged":[[0,0,0,6],[1,0,2,0],[1,1,2,1],[1,4,2,4],[1,5,2,5],[1,6,2,6],[2,2,2,3]],"flags":["formulas_cached_only"]}
["Table S5. The details of gladiolus genotypes involved in the present study "]
["Genotype ID","Genotype name","S1","S2","Pop. ID","Cluster ID","PCoA "]
[null,null,"Q value"]
[1,"Anglia",{"v":0.909,"d":"0.91"},{"v":0.091,"d":"0.09"},"I","I","I","AD =","Admixture"]
[2,"Amethyst",0.92,0.08,"I","I","I","Subpopulation 1","S1"]
[3,"Pusa Gunjan",{"v":0.951,"d":"0.95"},{"v":0.049,"d":"0.05"},"I","I","I","Subpopulation 2","S2"]
[4,"Punjab Glad 1",{"v":0.834,"d":"0.83"},{"v":0.166,"d":"0.17"},"I","I","I"]
[5,"Aldebaran",{"v":0.931,"d":"0.93"},{"v":0.069,"d":"0.07"},"I","I","I"]
[6,"Fire Flame",{"v":0.927,"d":"0.93"},{"v":0.073,"d":"0.07"},"I","I","I"]
[7,"Tiger Flame",{"v":0.279,"d":"0.28"},{"v":0.721,"d":"0.72"},"II","II","I"]
[8,"Kalima",0.95,0.05,"I","I","I"]
[9,"Neelima",{"v":0.949,"d":"0.95"},{"v":0.051,"d":"0.05"},"I","I","I"]
[10,"GS-2",0.89,0.11,"I","I","I"]
[11,"Praha",{"v":0.938,"d":"0.94"},{"v":0.062,"d":"0.06"},"I","I","I"]
[12,"Anjali",{"v":0.963,"d":"0.96"},{"v":0.037,"d":"0.04"},"I","I","I"]
[13,"Malaviya Shatabdi",{"v":0.877,"d":"0.88"},{"v":0.123,"d":"0.12"},"I","I","I"]
[14,"Roshni",{"v":0.912,"d":"0.91"},{"v":0.088,"d":"0.09"},"I","I","I"]
[15,"Yellow Stone",{"v":0.331,"d":"0.33"},{"v":0.669,"d":"0.67"},"AD","II","I"]
[16,"Dhanvantari",{"v":0.909,"d":"0.91"},{"v":0.091,"d":"0.09"},"I","I","I"]
[17,"Punjab Morning",{"v":0.902,"d":"0.90"},{"v":0.098,"d":"0.10"},"I","I","I"]
[18,"Rose Supreme",0.12,0.88,"II","II","II"]
[19,"Punjab Glance",{"v":0.521,"d":"0.52"},{"v":0.479,"d":"0.48"},"AD","I","I"]
[20,"Sancerre",{"v":0.643,"d":"0.64"},{"v":0.357,"d":"0.36"},"AD","I","I"]
[21,"Pusa Shagun",{"v":0.096,"d":"0.10"},{"v":0.904,"d":"0.90"},"II","II","I"]
[22,"Punjab Glad 2",{"v":0.288,"d":"0.29"},{"v":0.712,"d":"0.71"},"II","II","I"]
[23,"Pusa Shabnam",{"v":0.782,"d":"0.78"},{"v":0.218,"d":"0.22"},"I","I","I"]
[24,"Punjab Dawn",{"v":0.939,"d":"0.94"},{"v":0.061,"d":"0.06"},"I","I","I"]
[25,"Arka Tilak",{"v":0.707,"d":"0.71"},{"v":0.293,"d":"0.29"},"I","I","I"]
[26,"Victor",{"v":0.153,"d":"0.15"},{"v":0.847,"d":"0.85"},"II","II","I"]
[27,"Pusa Lohit",{"v":0.672,"d":"0.67"},{"v":0.328,"d":"0.33"},"AD","I","I"]
[28,"Malaviya Kiran",{"v":0.519,"d":"0.52"},{"v":0.481,"d":"0.48"},"AD","I","I"]
[29,"Pusa Manmohak",{"v":0.062,"d":"0.06"},{"v":0.938,"d":"0.94"},"II","II","I"]
[30,"Pusa Sindhuri",{"v":0.151,"d":"0.15"},{"v":0.849,"d":"0.85"},"II","II","I"]
[31,"Arka Nazrana",{"v":0.904,"d":"0.90"},{"v":0.096,"d":"0.10"},"I","I","I"]
[32,"Nova Lux",{"v":0.055,"d":"0.06"},{"v":0.945,"d":"0.95"},"II","II","I"]
[33,"Euro Vision",{"v":0.164,"d":"0.16"},{"v":0.836,"d":"0.84"},"II","II","I"]
[34,"Lady Jane",{"v":0.513,"d":"0.51"},{"v":0.487,"d":"0.49"},"AD","I","I"]
[35,"Jacksonville Gold",{"v":0.183,"d":"0.18"},{"v":0.817,"d":"0.82"},"II","II","I"]
[36,"Punjab Lemon Delight",{"v":0.475,"d":"0.48"},{"v":0.525,"d":"0.53"},"AD","II","I"]
[37,"Arka Aayush",{"v":0.879,"d":"0.88"},{"v":0.121,"d":"0.12"},"I","I","I"]
[38,"Pusa Sringarika",{"v":0.821,"d":"0.82"},{"v":0.179,"d":"0.18"},"I","I","I"]
[39,"Pusa Red Valentine",{"v":0.957,"d":"0.96"},{"v":0.043,"d":"0.04"},"I","I","I"]
[40,"Neel Rekha",{"v":0.953,"d":"0.95"},{"v":0.047,"d":"0.05"},"I","I","I"]
[41,"Yellow Star",{"v":0.949,"d":"0.95"},{"v":0.051,"d":"0.05"},"I","I","I"]
[42,"Creamy Green",{"v":0.965,"d":"0.97"},{"v":0.035,"d":"0.04"},"I","I","I"]
[43,"Friendship",{"v":0.972,"d":"0.97"},{"v":0.028,"d":"0.03"},"I","I","II"]
[44,"Pink Lady",0.88,0.12,"I","I","II"]
[45,"Arka Sapna",{"v":0.108,"d":"0.11"},{"v":0.892,"d":"0.89"},"II","II","II"]
[46,"Arka Kesar",{"v":0.918,"d":"0.92"},{"v":0.082,"d":"0.08"},"I","I","II"]
[47,"Pusa Suhagin",{"v":0.958,"d":"0.96"},{"v":0.042,"d":"0.04"},"I","I","II"]
[48,"Pusa Urmi",{"v":0.905,"d":"0.91"},{"v":0.095,"d":"0.10"},"I","I","II"]
[49,"Pusa Chirag",{"v":0.398,"d":"0.40"},{"v":0.602,"d":"0.60"},"AD","II","II"]
[50,"Nicole",{"v":0.217,"d":"0.22"},{"v":0.783,"d":"0.78"},"II","II","II"]
[51,"Pusa Archana",{"v":0.078,"d":"0.08"},{"v":0.922,"d":"0.92"},"II","II","II"]
[52,"Arka Darshan",{"v":0.291,"d":"0.29"},{"v":0.709,"d":"0.71"},"II","II","II"]
[53,"Plumtart",{"v":0.308,"d":"0.31"},{"v":0.692,"d":"0.69"},"AD","II","II"]
[54,"Pusa Sarang",{"v":0.531,"d":"0.53"},{"v":0.469,"d":"0.47"},"AD","I","II"]
[55,"Fidelio",{"v":0.033,"d":"0.03"},{"v":0.967,"d":"0.97"},"II","II","II"]
[56,"Urmil",{"v":0.048,"d":"0.05"},{"v":0.952,"d":"0.95"},"II","II","II"]
[57,"Lucky Shamrock",{"v":0.054,"d":"0.05"},{"v":0.946,"d":"0.95"},"II","II","II"]
[58,"Delhi Callianthus",{"v":0.076,"d":"0.08"},{"v":0.924,"d":"0.92"},"II","II","II"]
[59,"Pusa Urvashi",{"v":0.107,"d":"0.11"},{"v":0.893,"d":"0.89"},"II","II","II"]
[60,"Pusa Chandni",{"v":0.123,"d":"0.12"},{"v":0.877,"d":"0.88"},"II","II","I"]
[61,"Suchitra",{"v":0.128,"d":"0.13"},{"v":0.872,"d":"0.87"},"II","II","II"]
[62,"Surya Kiran",{"v":0.046,"d":"0.05"},{"v":0.954,"d":"0.95"},"II","II","II"]
[63,"Arka Amar",{"v":0.864,"d":"0.86"},{"v":0.136,"d":"0.14"},"I","I","II"]
[64,"True Love",{"v":0.046,"d":"0.05"},{"v":0.954,"d":"0.95"},"II","II","II"]
[65,"Peter Pear's",{"v":0.034,"d":"0.03"},{"v":0.966,"d":"0.97"},"II","II","II"]
[66,"Pusa Shweta",{"v":0.034,"d":"0.03"},{"v":0.966,"d":"0.97"},"II","II","II"]
[67,"Arka Poonam",{"v":0.183,"d":"0.18"},{"v":0.817,"d":"0.82"},"II","II","II"]
[68,"Pusa Srijana",0.05,0.95,"II","II","II"]
[69,"Pusa Gulal",{"v":0.092,"d":"0.09"},{"v":0.908,"d":"0.91"},"II","II","II"]
[70,"Vicki Lin",{"v":0.457,"d":"0.46"},{"v":0.543,"d":"0.54"},"AD","II","II"]
[71,"African Star",0.09,0.91,"II","II","II"]
[72,"Arka Gold",{"v":0.293,"d":"0.29"},{"v":0.707,"d":"0.71"},"II","II","II"]
[73,"Pusa Kiran",{"v":0.232,"d":"0.23"},{"v":0.768,"d":"0.77"},"II","II","II"]
[74,"Arka Aarti",{"v":0.873,"d":"0.87"},{"v":0.127,"d":"0.13"},"I","I","II"]
[75,"Arka Naveen",{"v":0.193,"d":"0.19"},{"v":0.807,"d":"0.81"},"II","II","II"]
[76,"Apple Blossom",0.07,0.93,"II","II","II"]
[77,"Punjab Pink Elegance",{"v":0.062,"d":"0.06"},{"v":0.938,"d":"0.94"},"II","II","II"]
[78,"Vink's Glory",{"v":0.039,"d":"0.04"},{"v":0.961,"d":"0.96"},"II","II","II"]
[79,"Pusa Bindiya",{"v":0.118,"d":"0.12"},{"v":0.882,"d":"0.88"},"II","II","II"]
[80,"Pusa Mohini",{"v":0.058,"d":"0.06"},{"v":0.942,"d":"0.94"},"II","II","II"]
[81,"Summer Sunshine",{"v":0.907,"d":"0.91"},{"v":0.093,"d":"0.09"},"I","I","II"]
[82,"Algarve",{"v":0.643,"d":"0.64"},{"v":0.357,"d":"0.36"},"AD","I","II"]
[83,"Malaviya Kundan",{"v":0.878,"d":"0.88"},{"v":0.122,"d":"0.12"},"I","I","II"]
[84,"C.P.G",0.82,0.18,"I","I","II"]

{"sheet":"Table S6","index":5,"n_rows":22,"n_cols":9,"rows_kept":22,"merged":[[21,0,21,8]],"flags":["formulas_cached_only"]}
["Table S6 Summary of marker wise genetic diversity measures for all the gladiolus genotypes"]
["Locus","Na","Ne","I","Ho","He","F","Nm","h"]
["G5",4,2.81,1.15,0.53,0.64,0.18,45.02,0.64]
["G7",4,1.28,0.47,0.09,0.22,0.57,7.08,0.22]
["G8",3,2.39,0.94,0.19,0.58,0.67,0.31,0.58]
["G9",2,1.13,0.23,0,0.11,1,3.7,0.11]
["G10",4,1.45,0.66,0.21,0.31,0.33,18.25,0.31]
["G11",3,2.51,0.99,0.3,0.6,0.5,2.25,0.6]
["G12",4,3.35,1.27,0.56,0.7,0.2,82.7,0.7]
["GP2",3,1.07,0.16,0.06,0.06,-0.03,65.33,0.06]
["GP4",6,1.74,0.79,0.53,0.42,-0.26,60.17,0.42]
["GP7",3,2.78,1.06,0.38,0.64,0.41,1.52,0.64]
["GP13",3,2.7,1.04,0.35,0.63,0.44,3.2,0.63]
["GP15",4,2.77,1.18,0.62,0.64,0.03,371.47,0.64]
["IM31",4,3.22,1.25,0.75,0.69,-0.09,10.44,0.69]
["IM86",2,1.16,0.26,0.12,0.14,0.12,4.16,0.14]
["IM108",2,1.61,0.57,0.51,0.38,-0.34,69.5,0.38]
["IM39",3,1.2,0.34,0.15,0.16,0.08,144.84,0.16]
["IM224",4,1.48,0.63,0.26,0.32,0.19,38.4,0.32]
["Mean ",3.41,2.04,0.76,0.33,0.43,0.24,54.61,0.43]
["±SE",0.24,0.2,0.09,0.05,0.05,0.08]
["Note: Na = observed number of alleles; Ne = number of effective alleles; I = Shannon's information index; Ho = observed heterozygosity; He = expected heterozygosity; F = fixation index; h= Nei’s gene diversity; Nm = gene flow"]

{"sheet":"Table S7","index":6,"n_rows":6,"n_cols":9,"rows_kept":6,"merged":[[0,0,0,8]],"flags":["formulas_cached_only"]}
["Table S7 Analysis of molecular variance (AMOVA) and F- statistics differentiating subpopulations"]
["Source","df","SS","MS","Est. var.","variance (%)","P value","F-statistics","value"]
["Among subpopulations",1,44.38,44.38,0.46,{"v":0.0986,"d":"9.86%"},0,"Fst",0.1]
["Among individuals within subpopulations",82,482.35,5.88,1.7,{"v":0.3655,"d":"36.55%"},0,"Fis",0.41]
["Among individuals within total population",84,209,2.49,2.49,{"v":0.5359,"d":"53.59%"},0,"Fit",0.46]
["Total",167,735.72,null,4.65,{"v":1,"d":"100%"}]

{"sheet":"Table S8","index":7,"n_rows":11,"n_cols":3,"rows_kept":11,"merged":[[0,0,0,7],[10,0,10,8],[11,0,11,2]],"flags":["formulas_cached_only"]}
["Table S8 Summary of genetic differentiation and heterozygosity statistics between subpopulations"]
["Sub populations","S1","S2"]
["Sample size (N)* ",35,37]
["Na","3.12±0.26","3.12±0.17"]
["Ne","1.99±0.21","1.84±0.17"]
["I","0.73±0.10","0.69±0.08"]
["Ho","0.34±0.05","0.32±0.06"]
["He","0.41±0.06","0.39±0.05"]
["F","0.12±0.06","0.22±0.09"]
["Polymorphic loci (%)",{"v":0.9412,"d":"94.12%"},{"v":1,"d":"100.00%"}]
["Note: Na = observed number of alleles; Ne = number of effective alleles; I = Shannon's information index; Ho = observed heterozygosity; He = expected heterozygosity; F = fixation index; h= Nei’s gene diversity; Havg = average heterozygosity; Nm = gene flow. * 12 genotypes were excluded from both subpopulations as they were assigned as admixture."]

{"sheet":"Fig S1","index":8,"n_rows":18,"n_cols":1,"rows_kept":18,"flags":["formulas_cached_only"]}
{"$rows_empty":17}
["Fig. S1 Estimation of best subpopulations (ΔK=2) from an assumed range of 1–10 based on Evanno method."]

{"sheet":"Fig. S2","index":9,"n_rows":22,"n_cols":2,"rows_kept":22,"merged":[[21,1,21,10]],"flags":["formulas_cached_only"]}
{"$rows_empty":21}
[null,"Fig. S2 Two dimensional PCoA scatter plot showing composition of two clusters; bullets in blue color with numbers represent genotypes of subpopulation 1 whereas bullets in orange colour with numbers indicate genotypes of subpopulation 2 as given in Table S5"]