Software is located at https://github.com/flemingtonlab/LongReadAnnotation Access to sequencing data is as follows: All raw data files for long-read ONT sequencing have been deposited in the NCBI GEO Archive under accession number GSE277022 (Link: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE277022. Token to access: olqxmcgehtanvgt).