Project info 1

Project info 1 Experiment Date2024-04-07 Experiment ID Notebook ID Project Experimenter Protocol Formalin test phase 1 Control 66 65 59 56 62 60 Cyt 56 54 53 60 61 54 Uri 59 56 55 56 53 56 Gaba 49 48 50 53 48 54 Cyt+Uri+Gaba 41 38 39 38 43 40

Formalin test phase 2 Control 70 74 72 68 66 71 Cyt 66 67 66 63 62 61 Uri 65 65 63 68 67 61 Gaba 58 57 62 61 55 54 Cyt+Uri+Gaba 47 48 49 44 52 41

Von fry formalin Basal 30 min 60 min 120 min Intact 0.7 0.68 0.685 0.688 0.68 0.69 0.695 0.694 0.73 0.71 0.705 0.706 0.66 0.72 0.715 0.712 0.74 0.67 0.675 0.682 0.71 0.73 0.725 0.718 Control 0.18 0.18 0.185 0.192 0.19 0.19 0.19 0.196 0.2 0.2 0.195 0.204 0.21 0.21 0.2 0.208 0.42 0.175 0.205 0.19 0.21 0.245 0.225 0.21 Cyt 0.21 0.222 0.244 0.255 0.22 0.231 0.252 0.263 0.24 0.249 0.268 0.277 0.25 0.258 0.276 0.285 0.2 0.213 0.236 0.248 0.26 0.267 0.284 0.292 Uri 0.19 0.246 0.257 0.268 0.2 0.253 0.264 0.274 0.22 0.267 0.276 0.286 0.23 0.274 0.283 0.292 0.18 0.239 0.25 0.262 0.24 0.281 0.29 0.298 Gaba 0.185 0.259 0.31 0.301 0.1925 0.2645 0.315 0.3055 0.2075 0.2755 0.325 0.3145 0.215 0.281 0.33 0.319 0.1775 0.2535 0.305 0.2965 0.2225 0.2865 0.335 0.3235 Cyt+Uri+Gaba 0.222 0.29 0.358 0.369 0.226 0.295 0.364 0.3745 0.234 0.305 0.376 0.3855 0.238 0.31 0.382 0.391 0.218 0.285 0.352 0.3635 0.242 0.315 0.388 0.3965

P-CREB 30 min after fomalin Control 85 87.5 92.5 95 82.5 97.5 Formalin 285 292.5 307.5 315 277.5 322.5 Cyt 258 264 276 282 252 288 Uri 250 255 265 270 245 275 Gaba 203 206.5 213.5 217 199.5 220.5 Cyt+Uri+Gaba 165 167.5 172.5 175 162.5 177.5

P-CREB 5 weeks after STZ Normal 97 98.5 101.5 103 95.5 104.5 Diabetic 288 294 306 312 282 318 Diabetic+Cyt 274 279.5 290.5 296 268.5 301.5 Diabetic+Uri 273.5 278.75 289.25 294.5 268.25 299.75 Diabetic+Gaba 241 245 253 257 237 261 Diabetic+Cyt+Uri+Gaba 225 228.5 215.5 229 221.5 222.5

Naloxone Formalin 68 69 71 72 67 73 Formalin+Cyt +Nal 61.4 62.2 63.8 64.6 60.6 65.4 Formalin+Uri+ Nal 60.6 61.3 62.7 63.4 59.9 64.1 Formalin+Gaba+ Nal 67.6 68.3 69.7 70.4 66.9 71.1 Formalin+Cyt+Uri+Gaba +Nal 56.6 57.3 58.7 59.4 55.9 60.1

Yohimbine Formalin 73.4 74.1 75.5 76.2 72.7 77.3 Formalin+Cyt+ Yoh 72.6 73.3 74.7 75.4 71.9 76.1 Formalin+Uri+ Yoh 68.5 69 70.5 71 67.5 72.5 Formalin+Gaba+ Yoh 61.5 62 63.5 64 60.5 65.5 Formalin+Cyt+Uri+Gaba+ Yoh 56.5 57 58.5 59 55.5 60.5

Methsergide Formalin 68 69 70 71 69 71 Formalin+Cyt+ Meth 67.5 68 69.5 70 68.5 70.5 Formalin+Uri+ Meth 66.5 67 68.5 69 67.5 69.5 Formalin+Gaba+ Meth 60.5 61 62.5 63 61.5 63.5 Formalin+Cyt+Uri+Gaba+ Meth 58.5 59 60.5 61 59.5 61.5

p-CREB naloxone Formalin 97.5 98 99.5 100 98.5 100.5 Formalin+Cyt+ Nal 68.5 69 70.5 71 69.5 71.5 Formalin+Uri+ Nal 66.5 67 68.5 69 67.5 69.5 Formalin+Gaba+ Nal 96.5 97 98.5 99 97.5 99.5 Formalin+Cyt+Uri+Gaba+ Nal 65.5 66 67.5 68 66.5 68.5

p-CREB yohimbine Formalin 97.5 98 99.5 100 98.5 100.5 Formalin+Cyt+ Yoh 95.5 96 97.5 98 96.5 98.5 Formalin+Uri+ Yoh 84.5 85 86.5 87 85.5 87.5 Formalin+Gaba+ Yoh 77.5 78 79.5 80 78.5 80.5 Formalin+Cyt+Uri+Gaba+ Yoh 74.5 75 76.5 77 75.5 77.5

p-CREB Methsergide Formalin 94.5 95 96.5 97 95.5 97.5 Formalin+Cyt+ Meth 93.5 94 95.5 96 94.5 96.5 Formalin+Uri+ Meth 92.5 93 94.5 95 93.5 95.5 Formalin+Gaba+ Meth 80.5 81 82.5 83 81.5 83.5 Formalin+Cyt+Uri+Gaba+ Meth 78.5 79 80.5 81 79.5 81.5