LOCUS       BRBV_LAV-238002        7319 bp DNA     linear   SYN 12-DEC-2023
DEFINITION  Contig1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 29, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mat_peptide     468..1088
                     /product="Leader"
                     /label=Leader
     primer_bind     complement(543..561)
                     /label=BRBV-238002-seq-582R
     primer_bind     complement(878..905)
                     /label=BRBV-238002-Nested-5RACE
     mat_peptide     1089..1364
                     /product="VP4"
                     /label=VP4
     primer_bind     complement(1157..1176)
                     /label=BRBV-Ibaraki2-seq-1197R
     primer_bind     complement(1228..1255)
                     /label=BRBV-238002-5RACE-R
     mat_peptide     1365..2048
                     /product="VP2
                     "
                     /label=VP2
     mat_peptide     2049..2705
                     /product="VP3"
                     /label=VP3
     primer_bind     2272..2287
                     /label=BRBV-Ibaraki2-seq-2289F
     mat_peptide     2706..3362
                     /product="VP1"
                     /label=VP1
     mat_peptide     3363..3479
                     /product="2A"
                     /label=2A
     mat_peptide     3480..3857
                     /product="2B"
                     /label=2B
     primer_bind     3648..3665
                     /label=BRBV-Ibaraki2-seq-3674F
     mat_peptide     3858..4805
                     /product="2C"
                     /label=2C
     primer_bind     4211..4230
                     /label=BRBV-Ibaraki2-seq-4238F
     primer_bind     complement(4388..4407)
                     /label=BRBV-Ibaraki2-seq-4434R
     primer_bind     4695..4714
                     /label=BRBV-Ibaraki2-seq-4722F
     mat_peptide     4806..5204
                     /product="3A"
                     /label=3A
     primer_bind     complement(4979..4996)
                     /label=BRBV-Ibaraki2-seq-5023R
     mat_peptide     5205..5279
                     /product="3B"
                     /label=3B
     mat_peptide     5280..5903
                     /product="3C"
                     /label=3C
     primer_bind     complement(5484..5502)
                     /label=BRBV-Ibaraki2-seq-5529R
     mat_peptide     5904..7304
                     /product="3D"
                     /label=3D
     primer_bind     complement(5964..5980)
                     /label=BRBV-Ibaraki2-seq-6008R
     primer_bind     6172..6198
                     /label=BRBV-3RACE-F
     primer_bind     6305..6322
                     /label=BRBV-Ibaraki2-seq-6332F
     primer_bind     complement(6514..6529)
                     /label=BRBV-Ibaraki2-seq-6560R
     primer_bind     6743..6761
                     /label=BRBV-238002-seq-6742F
     primer_bind     6753..6772
                     /label=BRBV-Ibaraki2-seq-6780F
     primer_bind     complement(6994..7012)
                     /label=BRBV-Ibaraki2-seq-7038R
ORIGIN
        1 cactgtctac atagtgagac tgaccaagac aacaactcca aatgctgaac ttcgaaagtc
       61 tgaactggat ccgtaactgg acatgctagg gtaaggaact cctccctggt gacagggacc
      121 ttactggcca aaagccctgc tctcacgagc ctagcatgtt gttgaccccg aactgtccaa
      181 tttagttagt acaaggaacg catttgtcgt ggttgctctg aactcggttt ctagtgaaac
      241 agcctaagga tgccctccag gtaccctggg gtaacaagag acacccagga tctgaggagg
      301 ggaccgccta gagcgattta aaaaacttct aagctgttat ggtgaccaga ggctggcacc
      361 tttcatttta acaaactttt taaactgatc acgaacaaaa ggataacgtt tttgtagacc
      421 ttcaaacttg ttgcacgggt actactccta ccactgacaa cttttgtatg acaactgaac
      481 ggcttctgca ccacctcctt atagacacta tacacaaaaa gataaacaag aacaccaaaa
      541 tggcacttga acttggtttc aataacactc ttatcactgc tgaccttgat ggaaaagaaa
      601 aagtgatgca cggactacct aacaaacatg acaactgttg gctcaacgct ctcatgcaaa
      661 tgactaactg ggttggagaa ggcttcttca gaaacaccta cgacaatcca gacctcatcc
      721 ctcagaccat taaattcctt actgactaca caggaatcga tcttagctac ggaggacccc
      781 cttcaattgt tctctacaaa ataagagatc tccttgatac gaaagtgggc acttcaaaag
      841 aacccggaga ctacgtcgtg tcctgtcaag gcacatactg tcttgcggac atggaagcag
      901 gcgttttctt tgacggagag gaacacgcag ttttttacgc ttgtactgca cttggatgga
      961 taaagattga tgatgagact atcactagag aaataccaga cccagccaat gtcttggtct
     1021 ttgtcccttg ggataaggag accatttgtg actacgacga aaacttcttt caccagactt
     1081 acttaaaagg cgcaggctcc tctaaacctc agtctggcaa cgtcaatgaa agtggtaact
     1141 ctggctccat aatcaacaac tattacatgc aacagtacca gaactccatt gacgctacta
     1201 ttggcgacaa gaccacagaa ggtggttctg gttctggcga tacggcaggc agtgccactc
     1261 acaataacac caccaaagag gacaagggta aggacgattg gttctctagt ctcatgtcag
     1321 gggttggcag cgcaattccc ggtgctgtca tgggtcttct tgcggacaag aaaactgagg
     1381 agaccaccaa attagaggac cgcatcatga ccacccgaca ccgcacagac atcaccacga
     1441 cgcagtcaac agtgggcatc gttcagggtt atgcccattt cgaacgggac cacatcccgg
     1501 ctgctagtgg cactcatgaa cacgttgggc acgtctcgcg tatttacatg aagaaactct
     1561 tctcttggac tttaaccaac ggcgcagggt actaccacgc ctatcctctc ccagatgtca
     1621 tcttctccga agctagagaa tatcacaacc tgctcaagtc ctacgctctc taccgtaacg
     1681 gttgggaagt tcatgtctca gtggtgtcga caatgtacca ctctggctgt ttaattgttg
     1741 ctatggtccc tgaatggatg aaagaggaga ccaatggtaa tacttttgac cctcactttg
     1801 cacagttaac cttatacccg caccaactta tcaacctgag gactaacacc acagcatctc
     1861 tgcgtgtgcc ctacgtaggt gcaactgata tggacgacca caggctccac aaagcttgga
     1921 ctcttgttgt cggtgtggtc gttcccatga aaatttcaaa cggtggtagt gacctcacaa
     1981 accttgacgt tagagctagt ttcacacctc ttaacgtcaa ggttgctggc cctatgccga
     2041 ataaacaggg aatcatacca gtggcagcca aagccggcta ctctggtttt tgtaccacca
     2101 gccctattac tgctgatcca gtctacggca aggttgtaaa ccccccgaga agacacatac
     2161 caggacgctt cactaatttt cttgacgtgg cagacgcttg tcctaccatg tccagatttg
     2221 taaacaaacc cagtgtgacc accacctccg gagctgcaga aaaacttata gccaccattg
     2281 atgtcagcct cgtctcacac gagatgtcat tcacttacct ggctggtctt tcctccctct
     2341 acgcccagta ccgaggaagt atcaacatgc actgcattta cacaggtttc gtcagcgaca
     2401 aagccaagtt tttgattgct tttataccgc caggtgctga tctaccaagt acactcagtg
     2461 aggctcaaca ctgcataaca atggagtggg acaccggctt gaattctgaa acagtgttca
     2521 atataccgta catatcccaa acttactaca cttccacgca ctccagtgag gctgacatcg
     2581 gtaacgtgtc tggcagggta caaatatacc aagttacagc accttcctcc acttcagaac
     2641 ttctcgttct gttctcttct ggtagtgact tccagcttcg ctgccccgtt gaaccagtca
     2701 aacaagttac ggatgttggg gaaaccggac actacaaaac actggacgcg acacaacaga
     2761 atggagacag atccaacacc ttccgtctac acactgaagt ttcttttgct cttgaccggt
     2821 acgcgttgct tgatgttgta tctggaaacg caagcagtac acacaagcgg tacactagcc
     2881 ttgatcccac caagttacct gttgacacat acatgaggaa gcttattgac tcttgcacct
     2941 actacttcgc tgacctggaa attacagtca acgcaaaggg agaagtaccc gcatgggcca
     3001 acgtgacttg gcaccctgtt ggcgctcctc acacttttac taacgacaac ttgccagatg
     3061 aagccagtga ctacctgact actaactcta acgttagtgt cggattcact gggccgtcca
     3121 gcggtggtag catcgccaca ttcgcagtgc cttatacctc tttttacagg gttctgccta
     3181 cgaagtacgc tggcaggact ctttttgtaa aacaggaaca atttgaacca ttcaaccatg
     3241 gaggctttgg tgaggtcatt atcataggga aagaggctac caaacacaga atactcatca
     3301 gactgaaaag agcagagatg tactgtcctc gagctctgta cccttccgct tcagtgacaa
     3361 cactttctac cagacgaaag ctcaggttaa caggtgaaat tgtcaaacaa ggagccacaa
     3421 acttcgagct tttacaacgt gctggtgatg tagaaaccaa tccgggaccc gcatctttca
     3481 gtaaactaat tgaggatttt ggctgcctgt ccaactctat ggaagaaatc gccagacaca
     3541 tcggtgactt caaagtcatg atgaaaggag ctggaccatg gtataaagcc tttaaatacc
     3601 tctggaaagt ggccacagtt atcgtggcta ttaccaggac taaagataca gtccttgttg
     3661 gcatgctctt ggctgacatt gggttggagg tttttgacac tagagtcatg atggacaatc
     3721 tcgtggacag attcaaacca tacttccatg ttagtccacc caaatttgac tttaaaactg
     3781 aagtcttgga caaggtcagg gatttctttg ctactgaaga ggaagaagag ttcgacgaca
     3841 ctaacccctt taaacaaatt tctttgaaaa acgtcaatga catcttcaac ctcgttaaga
     3901 acggtcagtg gcttatgagt tttttcctct cacttagaga ctggtttcga acttggcttg
     3961 aaagcgagga gaagttcatt acctaccatg accttgtgcc tcagataatt gagcaccaag
     4021 aaaagctttt ggtgcctgat gagtatgctg aggcgcacaa ctggttagaa agaaaaagag
     4081 aggttctcct ccaggccaac cagtatgctc ttgctaaact ctgtgaaccc aaagttggac
     4141 caccacctga aacaaggccc gaaccagtgg ttattttgtt cagaggggac tctggtcaag
     4201 ggaaaagttt tctctctaat ctgattgctc aggcgttgtc caaacttctc actggtagag
     4261 ttgattctat ctggtcatgt cctccagacc ctgaccactt tgacgggtat cgaggtcaga
     4321 aggttgtgat tatggacgac cttggacaaa accctgatgg tacagacttc aaatattttg
     4381 ctcagatggt ttctaccaca gctttcatac caccaatggc agctcttgag gataaaggta
     4441 aggtttttaa ctcgccagta atcattgcca ccaccaacat gcacgaacac ttcacaccaa
     4501 aaacgatggc gtgtcctggt gctcttgttc gccgttttac ctatgactat gttcttgcag
     4561 caaagaagcc atacatcagg gagaaaactg aaacactcaa cgtcaggaag gctctgaacg
     4621 ccaccgggca agaatgtccg tgtgggctct ttgaatttga ctgtcctctt ctcaatggag
     4681 aggcaataga tttgactcca atcagagaca cacccacggt tgagagtgtt tacgaactca
     4741 tagagcttgt gtacaacgag gtcatggacc gcagaactgt ctcagacctc aagatactca
     4801 aacaaatggg taaatacaca acccttgaca acatcaaaag acacaaacta acgttcacac
     4861 ccttttctgg ccaaggatac caaccaggta gagacatcaa cgccagtcca gagatgcagg
     4921 agaaactact gaaatacctg gttaaacacg aacaccttga cgctgctctt aatttttaca
     4981 acgaggagtg tgacgaggag gttcgtgcta aatggggctc ttctattggt gaatatctca
     5041 aagtgaagac cctttggatg aaagtgaaga agtattcgca tcttttcctc accggtctta
     5101 tgctcatagg aaacatgctt cttctttacc ttaataacag aacaccagaa gagaaaaaga
     5161 aaaagaagaa aaataaaaca gaagaagaca acaccatcaa agaagggcct tatgggggtc
     5221 aagcaaaacc accagttaag gttgacaagt tgaaagtgaa tcctctgatt gcaaccgaga
     5281 gtgggaaccc accaactgac atgcagatgg tagtactaaa aaacacacaa ccaatttcat
     5341 tgattagaga cgggcaaatt gttgctactt gttgtgctct tggtgttttt ggaactacct
     5401 atctagtccc tcaccacctc tttgaagaga attttgaaac tcttgttata ggggacagac
     5461 acttaaaaga acaagattac aaacttgaca cttttgagct cagggacgga aaggtcagtg
     5521 atgttgccgc catcacccta aataaagggg cgcgtgttcg tgacatcacc tcccacttcc
     5581 gagacgaggt caaagtcact aagaattcac cagttgttgg atgtgtcaag aacacaacaa
     5641 ttggtcagct tgtcttcaac ggaacagctg ctggctttaa agatcacata atctgttctg
     5701 acggtgacac tctgcctaac atgtttgtct acactgctaa cacacagtat ggttattgtg
     5761 ggagtggtat acttgttaaa gatggttctc acacagtcat cataggtatc catagtgcag
     5821 gaggtaacgg aaaaggttat gctagttgtg tcactcgttc cgcactcctc gcgctcaggg
     5881 gcagagaaag acctgaactt gaaggactta tgttgggtga acaacctgga gagaaagtcc
     5941 acgtttctcg aaagaccaaa cttgctccca ctgttgctta cggtgttttc cgtccaaact
     6001 acggacctgc agcccttagt aattctgaca ccagactaaa cgaaggcgtt gtactcgaca
     6061 atgtcatttt cagcaaacac aagtccaatg ttgttcttaa cgagaaagac cttgctcttt
     6121 accgtctctg tgctgctgag tacgcctcac atcttcacaa cgttcttggc aagactggcg
     6181 cgcctctaac caacgtcgag gccgtacttg gtatcgacgg acttgacgcc atggagccca
     6241 acactgctcc tggcctacct tgggctctcc aacacaagcg cagacacgac cttcttgatt
     6301 ttactaccgg cgagatgcaa cctgccctgg ctgagagatt tgagcagctt gagaatatgg
     6361 actacacttt tgagtgtcag acctttttga aagatgaaat aagacctaac gagaaggtta
     6421 aagccgggaa aactagaata gtggacgttc tccctctgga acacattgtc ttttctcgca
     6481 aatacctggg tagattttgt gccgctatgc accgcaactg cggtccaaac atcggttccg
     6541 cagttggttg tgacccagac gtggcttggc aagaatttgg cactcacttt tcccagtaca
     6601 agaatgtttg ggccattgat tacacagctt ttgactcctg tcattcaact caactcatgt
     6661 cagttatggc tgatgaagtt ttctcagacg cgtacggatt tgacgagagg gcacgttacg
     6721 ccgtgaaatc tttgtgcaag acagtccatg cttatgaaga caagcgtatc actatagatg
     6781 gtggtttgcc ttcaggctgt agcgctacca gcattcttaa cacagttctc aacaacatct
     6841 acgtgatgtt tgccctcaag cggacttatc ccaacacaga gatgagtgac tactcactca
     6901 ttgcatatgg tgatgacctg gttttggctt cagattttga ttacgacatc aaccttgtaa
     6961 agaaatcttt tgcagtactt ggacacacca taacacctga agacaagtct gacaatggtt
     7021 ttactttggg caggagcatt acagatgtta catttcttaa acgcgctttt gaacgcgatt
     7081 gtgtcctagg gttttacaaa cccgtgatgg attccaaagt cttggaggcc atcctgtcct
     7141 tcgcacggcg tggcacactt caggagaagc taacctcagt agcgggtctc gctttccaca
     7201 gtggagaaga ggaatacaac agacttttct ctccttttgt gggaagtttt gagattccgt
     7261 catacaggtg ccttcgtctg agatgggtac acaaaatgtc aaattgaatt gaacataac
//
