LOCUS       BRBV_IBA-2211-2        7361 bp DNA     linear   UNA 29-JAN-2024
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   lein4
  TITLE     Direct Submission
  JOURNAL   Exported Jan 29, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7361
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=BRBV-Ibaraki2-seq-1F
     primer_bind     486..504
                     /label=BRBV-Ibaraki2-seq-486F
     mat_peptide     489..1109
                     /product="Leader"
                     /label=Leader
     primer_bind     complement(564..582)
                     /label=BRBV-Ibaraki2-seq-582R
     primer_bind     962..980
                     /label=BRBV-Ibaraki2-seq-962F
     primer_bind     complement(1031..1058)
                     /label=BRBV-Ibaraki2-nested-5RACE
     mat_peptide     1110..1385
                     /product="VP4"
                     /label=VP4
     primer_bind     complement(1113..1141)
                     /label=BRBV-Ibaraki2-5RACE-R
     primer_bind     complement(1178..1197)
                     /label=BRBV-Ibaraki2-seq-1197R
     mat_peptide     1386..2069
                     /product="VP2"
                     /label=VP2
     primer_bind     complement(1521..1540)
                     /label=BRBV-Ibaraki#2-2nd-seq-1540R
     primer_bind     1703..1720
                     /label=BRBV-Ibaraki2-seq-1703F
     primer_bind     1732..1750
                     /label=BRBV-Ibaraki#2-2nd-1732F
     primer_bind     complement(1821..1840)
                     /label=BRBV-Ibaraki2-seq-1840R
     primer_bind     complement(1908..1927)
                     /label=BRBV-Ibaraki#2-2nd-1927R
     mat_peptide     2070..2726
                     /product="VP3"
                     /label=VP3
     primer_bind     2289..2308
                     /label=BRBV-Ibaraki2-seq-2289F
     primer_bind     complement(2416..2434)
                     /label=BRBV-Ibaraki2-seq-2434R
     primer_bind     2712..2731
                     /label=BRBV-Ibaraki2-seq-2712F
     mat_peptide     2727..3383
                     /product="VP1"
                     /label=VP1
     primer_bind     complement(2835..2852)
                     /label=BRBV-Ibaraki2-seq-2852R
     primer_bind     3159..3178
                     /label=BRBV-Ibaraki2-seq-3159F
     primer_bind     complement(3264..3281)
                     /label=BRBV-Ibaraki2-seq-3281R
     mat_peptide     3384..3506
                     /product="2A"
                     /label=2A
     mat_peptide     3507..3884
                     /product="2B"
                     /label=2B
     primer_bind     3674..3692
                     /label=BRBV-Ibaraki2-seq-3674F
     primer_bind     complement(3816..3835)
                     /label=BRBV-Ibaraki2-seq-3835R
     mat_peptide     3885..4832
                     /product="2C"
                     /label=2C
     primer_bind     4238..4257
                     /label=BRBV-Ibaraki2-seq-4238F
     primer_bind     complement(4415..4434)
                     /label=BRBV-Ibaraki2-seq-4434R
     primer_bind     4722..4741
                     /label=BRBV-Ibaraki2-seq-4722F
     mat_peptide     4833..5231
                     /product="3A"
                     /label=3A
     primer_bind     complement(5006..5023)
                     /label=BRBV-Ibaraki2-seq-5023R
     mat_peptide     5232..5306
                     /product="3B"
                     /label=3B
     primer_bind     5253..5271
                     /label=BRBV-Ibaraki2-seq-5253F
     mat_peptide     5307..5930
                     /product="3C"
                     /label=3C
     primer_bind     complement(5511..5529)
                     /label=BRBV-Ibaraki2-seq-5529R
     primer_bind     5828..5845
                     /label=BRBV-Ibaraki2-seq-5828F
     mat_peptide     5931..7331
                     /product="3D"
                     /label=3D
     primer_bind     complement(5991..6008)
                     /label=BRBV-Ibaraki2-seq-6008R
     primer_bind     6199..6225
                     /label=BRBV-3RACE-F
     primer_bind     6332..6349
                     /label=BRBV-Ibaraki2-seq-6332F
     primer_bind     complement(6541..6560)
                     /label=BRBV-Ibaraki2-seq-6560R
     primer_bind     6780..6799
                     /label=BRBV-Ibaraki2-seq-6780F
     primer_bind     complement(7021..7039)
                     /label=BRBV-Ibaraki2-seq-7038R
     primer_bind     complement(7341..7361)
                     /label=BRBV-Ibaraki2-seq-7361R
ORIGIN
        1 cttgtggcgg cttgtggcac tgcatgcata gtggagtcga ccaagacaac agcaccaaac
       61 actgaacctc gaacgtctaa actagatccg tagctggaca tgttagggca aggaataccc
      121 ccctggtaac agggaccttg ctggccaaaa gccctgctct atgagcctaa catgttgttg
      181 accccgaact gtctagtttt ggttagtaca gagaatttgt ttgttgtggt tcgtaagaac
      241 tcgacttcta gtgaaacagc ctaaggatgc cctccaggta ccccggggta acaagtgaca
      301 cccgggatct gaggagggga ttgccttgcg caatttaaaa aacttctaag ctgttacggt
      361 gaccagaggc tggcaccttt cattttaaaa aaaaaaactt ttcaattggt tacaaacaaa
      421 agaacatcgt ttttgcaggc tttcaaacgc gtcacacaag tactgctttt actgttgcca
      481 ctcaatttat gaccactgaa agacttttac accacctgtt gatagacacc atacacaaga
      541 aaataaacaa acttaacact atggcactcg aacttggttt caacaacacc ctcatcactg
      601 ctgatcttga cgggaatgag aagatcatgc atggtttgcc aaacagacac gacaattgtt
      661 ggctcaactc cctcatgcaa atgacaaact gggtcggtga aaaatttttt aaaaacacgt
      721 acgacaatcc cgaccttatc ccccagacca tcaaattttt gacagagtac actggtatag
      781 acctgagcta tggaggaccg ccctccatag tcttgtacaa gatcagagac cttcttgaca
      841 caaaggttgg cacttctaaa gaacctggcg actacgtcgt ctcctgtcaa ggcaattact
      901 gtctagccga catgcaagcc ggtgttttca tggaaggcga ggaacacgca gttttctatg
      961 cttgtactgc tctcggttgg ataaagatag atgatgaaaa cattgtcagg tgcataccag
     1021 acccagcaaa cgtgctggtc tttgtcccgt gggacagcga aaccatttgt gactacgaca
     1081 ttgatttttt caaccaagtg tacttgaaag gcgctggctc gtcaaaaccg caatccggca
     1141 atgtcaacga aagtggtaat tctggttcta tagtaaacaa ctactacatg caacagtacc
     1201 agaattccat cgatgccact attggtgaca agacaaccga aggcggttca ggttctggtg
     1261 atacagctgg tagtgccact cacaacaata caaccaaaga agacaaaggc aaggatgact
     1321 ggttttctag tcttatgtct ggagttggta gtgctctccc tggagctgtc gtaggtctcc
     1381 ttgctgacaa gaaaaccgaa gaaaccacca aattggaaga ccgcatcatg tccacccgac
     1441 acagaacgga cattactact acgcagtcaa gtgtcggtgt tgtcaatggc tacgcgtttg
     1501 aagagaaaga ccaggttgtg gctgccagtg gtacacatga gcacatcagt catgtctctc
     1561 gtatctacat gaaaaaactc ttcaaatgga gtacggctga gcaagtggcc acttactatt
     1621 cctacacact cccagatcaa attcttcctg aggcaaagga ctacaaaaat cttctttcat
     1681 cctacgctct ttaccgtaac ggctgggagg ttcacgtgtc agtagtttcc accatctacc
     1741 actctggttg tttagtagtt gctctagtcc cagaattcac tccaggcaca acatctggca
     1801 gaacttacga tcccaatttt gcacaactca ctctatatcc acatcaattt ataaatttga
     1861 gaacgaacac cacagcttca atacgtgttc cttacgtagg agctactgac atggatgact
     1921 acagactcca ttcagcttgg acactgatcg tgggtgttgt tgttcctctc aagatacata
     1981 atggcaatag tgacctaacc gccatagatg ttcgagctag catcactccc actaacgtta
     2041 aggtggctgg gcctttgcct aacaaacaag gcatcatccc ggttgctgtc aaatctggtt
     2101 actctggttt ctctactaca agtcccatca cagcagatcc tatatatggg caggttgtta
     2161 acccaccaag gaggtacatt cctggtaggt ttactaattt cttggacgtg gctgacaagt
     2221 gccccactat ggctcgtttc gtctctaaac cctctgttac tactcaacag ggtgcatcag
     2281 aagtcctttt gtccacaatt gatgtcagtc tcgtttcaca cgaactttcc tacacttacc
     2341 ttgcaggact tgcctcactt tacgctcagt acagaggcag tatcaacatg cactgtattt
     2401 atacaggatt tgctagtgac aaggctaaat ttctgttagc ctatatacca cccggtgcgg
     2461 aacaaccacg tactctcagt gaagctcagc actgcatcac gcttgagtgg gacactggat
     2521 tgaattcaga aactgtgttt aacatacctt atttgtctca aacttactac acctcaacac
     2581 actccagcga ggccgatatt ggtaatgttt ctggcagagt gcagattttc caagtaacag
     2641 agacttctac accttctgaa cttcttgttt tgttttcatc tgggtctgac tttcaacttc
     2701 gttgtccaat tgaacctgtt aaacaagtca ctgacgtggg tgagaccggc aaatatgtca
     2761 ccctcgacgc cacccagcag aacggtttca aagcaaacac ttttcggtta cacacggacg
     2821 tagcctttgc tttggaccgc tacacgcaat tagctaatat taaaggcaac agtgctgaca
     2881 gccacaagac caccacgaac cttgacccaa ctaagctccc tcctaacact tttgtaaaaa
     2941 agttggttga ctcatgcacc tactacttct cagacctgga agtcactgtc aacgccaaag
     3001 gtactgtgcc agcttgggcc aatgttactt ggcacccagt atctgcccct cgttcgttca
     3061 cgaacaacaa ttaccctgat gaagacaatg acttcatgac agttaattct aacgccagtg
     3121 tcggtttcac aggtccttcg ggaagtgggt ctattgccac ttttgcaata ccttacactt
     3181 ccatataccg agttctaccg acaaagtatt ctggtagaac ccaattcacc cctgtcgaca
     3241 atttcaaggc actgagttac acgggttttg gcgagataac ggtggcgggt cttgaagaca
     3301 cctaccaccg agttctcatc agaatgaaaa gaacagaaat gtactgtccc agaccacttt
     3361 acccttcaac cactgtaaac acagtctcta ccagaagaaa gtttcccttg gtaagtgaaa
     3421 gtgttcctgt gagacgagga gctacaaact acagtctcct aaaacaggcc ggcgatgttg
     3481 agagcaaccc tggacccaca tctttcagca aactgattga agactttggt tgtttgtcta
     3541 attccatgga agaaatcgcc agacacattg gcgactttaa agtcatgatg aaaggggccg
     3601 ggccatggta caaagccttc aaatacctct ggaaagtggc cacagtcatt gtggctatta
     3661 ccaggactaa agacacagtt cttgttggca tgctcttggc tgacattggg ctggaggttt
     3721 ttgacaccag agtcatgatg gacaaccttg tagacagatt caaaccgtac tttcatgtta
     3781 gcccacccaa atttgacttc aaaaccgaag tcttggacaa agtcagagac ttctttgcca
     3841 ctgacgagga agaggagttt gacgacacta acccttttaa acaaatttct ttgaaaagtg
     3901 tcaacgacat cttcaacctc gttaagaacg gtcaatggct tatgagtttc ttcctctcac
     3961 ttagagactg gtttcgaact tggcttgaaa gtgaggagaa atttattaca taccacgacc
     4021 ttgtacctca gataattgaa caccaagaaa gactcttagt acctgacgaa tatactgaag
     4081 cacacaactg gctagaaagg aaaagagaag ttctcctcca ggccaaccag tatgctcttg
     4141 ctaaactctg tgaacccaaa gttggaccac cacccgaaac aaggcccgaa ccagtggtta
     4201 ttttgttcag aggggactct ggccagggga aaagttttct ctctaacctg attgctcaag
     4261 cgttgtccaa acttctcact ggtagagttg actctatctg gtcgtgtcct ccagaccctg
     4321 atcactttga cgggtatcga ggtcagaagg ttgtgattat ggacgacctc ggacaaaacc
     4381 ccgacggcgc agatttcaaa tattttgctc agatggtttc taccacagct ttcataccac
     4441 caatggcagc tcttgaggac aagggcaagg tttttaactc accagtgatc attgctacca
     4501 ccaacatgca cgaacacttc acaccaaaaa cgatggcgtg tcccggcgct cttgttcgcc
     4561 gttttaccta tgattatgtt cttgcagcaa agaagccata catcagggag aaaactgaaa
     4621 cactcaacgt caggaaggct ctgaatgcca ccgggcaaga atgtccgtgt gggctctttg
     4681 aatttgactg tccccttctc aatggagagg caatagattt gactccaatc agagacacac
     4741 ctacagttga aagtgtttac gaactcatag agcttgtgta caacgaggtc atggaccgaa
     4801 gaaccgtctc agacctcaaa atacttaaac aaatgggtaa atatacaacc cttgacaaca
     4861 ttaagaaaca caaaccgaca ttcacaccct tttctggcca tggataccaa ccaggcagag
     4921 acatcaacgc cagtccagaa atgcaggaga aactactgaa atacctggtt aaaaacgaac
     4981 accttgatgc tgctcttaat ttctacaacg aagagtgtga cgaggaggtt cgtgctaagt
     5041 ggggcccttc cattggcgaa tatcttaaag tgaaaaccct ttggatgaaa gtgaagaaat
     5101 attcacatct tttcctcacc ggtcttatgc ttataggaaa catgcttctt ctttacctca
     5161 acaacagaac accagaagaa aaaaagaaaa agaagaaaaa caaaacagaa gaagacagca
     5221 ccatcaaaga agggccttat ggaggtcaag caaagccacc agttaaagtt gacaaactga
     5281 aagtgaatcc tctgatcgca accgagagtg ggaacccacc aactgacatg cagatggtag
     5341 tgctaaaaaa cacacaacca atttcactga ttagagacgg gcaagttgtt gctacttgtt
     5401 gtgctcttgg tgtttttgga acgacctatc tagtccctta ccacctcttt gaagagagtt
     5461 ttgaaactct tgttataggg gacagacact taaaagaaca agattacaga cttgacactt
     5521 ttgaactcaa agacgggaag gtcagtgatg ttgccgccat caccctaaac aaaggggctc
     5581 gtgtgcgtga cattgtctcc cacttccgag atgaagtcaa agttaccaag aactcaccag
     5641 tcgttggatg tgttaagaac acaacagttg gtcagcttgt ctttaacgga acagctgctg
     5701 gctttaaaga tcacataatc tgttctgacg gtgacactct gcctaacatg tttgtctata
     5761 ctgctaacac acagtacggt tattgcggga gtggtatact tgtcaaagat ggttctcaca
     5821 cagtcatcat aggcatccac agtgcaggag gtaacggaaa aggttatgct agttgtgtca
     5881 ctcgttccgc actcctctca ctcaggggta gagaaagacc cgaacttgaa ggacttatgt
     5941 taggtgaaca acctggagag aaagtccacg tctctcgaaa gaccaaactt gctcccactg
     6001 ttgcttatgg tgtttttcgt ccaaattacg gacctgcagc ccttagcaat tctgacacca
     6061 gactaaatga aggtgttgtg cttgacgatg tcattttcag caaacacaag tccaatgtcg
     6121 ttctcaatga gaaagatctc gctctttacc gcctctgtgc tgctgagtac gcctcacatc
     6181 ttcacaacat tcttggcaag actggcgcgc ctctaaccaa cgtcgaggcc gtgcttggta
     6241 tcgacggact tgacgccatg gaacccaaca ccgctcctgg tctaccttgg gctctccaac
     6301 acaagcgtag acatgatctt cttgatttca ctaccggtga gatgcaacct gccctagctg
     6361 agagatttga gcaacttgaa aacatggact acacttttga gtgccaaacc tttttgaaag
     6421 acgagataag acctaacgag aaggtcaaag ccgggaagac caggatagtg gacgttcttc
     6481 ctctggaaca cattgtcttt tctcgcaaat acctgggtag gttttgtgct gctatgcacc
     6541 gcaactacgg tccaaatatt ggttccgcag ttggttgtga cccagatgtg gcttggcaag
     6601 aatttggcac tcacttttcc cagtacaaga acatttgggc cattgattac acggcttttg
     6661 actcctgtca ttcaacacaa ctcatgtcag ttatggctga cgaggttttc tcagacgcac
     6721 acgggtttga tgaaagggca cgttacgccg tgaaatcttt gtgtaagaca gttcatgctt
     6781 atgaggacaa gcgtatcact atagatggtg gtctgccttc aggctgtagt gccaccagca
     6841 ttcttaacac agtcctcaac aacatctatg taatgtttgc tctcaggcgg acttatccca
     6901 atacagagat gagtgactat tcactcattg catatggtga tgacctggtt ttggcttcag
     6961 atcttaatta cgacatcaat cttgtaaaga aatcttttgc agtacttgga cacaccataa
     7021 cacctgaaga caagtctgac aatggtttta ctttgggcaa gagcattaca gatgtcacat
     7081 ttctcaaacg cgcttttgaa cgcgattgtg tcctagggtt ttacaaaccc gtgatggatt
     7141 ccaaagtctt ggaggccatc ctgtccttcg cacggcgtgg cacacttcag gagaagctaa
     7201 cctcagtggc aggtctcgct ttccacagtg gagaagagga atacaacaga cttttctctc
     7261 cttttgtggg aagttttgag attccgtcat acaggtgcct tcgtctgaga tgggtacaca
     7321 aaatgtcaaa ttgaattgaa cataaccaaa ctaactacag t
//
