{"text_format":"3","workbook":"xlsx","n_sheets":1,"sheets":["Sheet1"],"read":"whole","date_system":"1900"}

{"sheet":"Sheet1","index":0,"n_rows":73,"n_cols":3,"rows_kept":73}
["Primer name","Sequence(5'~3')","Use"]
["EMB2279-endo-5’-UTR","GACGTTGACGATAGCCGAT","for qRT-PCR to detect the Endogenous  EMB2279 expression level"]
["EMB2279-endo-R","CCAACCTCCTTCGTGTTCA","for qRT-PCR to detect the Endogenous  EMB2279 expression level"]
["EMB2279-CDS-F","CACCATGGCGGTGACGATTTCG","for mutated EMB2279 CDS cloning"]
["EMB2279-CDS-R","TTACAAGTTATGTGTTCTGGTCTTC","for mutated EMB2279 CDS cloning"]
["A3-R2","CAGTGTAAGTCACCACGAG","for EMB2279 RT-PCR"]
["sot5-dCAPS-R","GTACATACGGTTGAGGATATG","for EMB2279 RT-PCR"]
["EMB2279-exon1-F","GAGTGGAACCAGGAGAATTG","for EMB2279 qRT-PCR"]
["EMB2279-exon1-R","GCTAACTTCTCGATTCTGTCT","for EMB2279 qRT-PCR"]
["EMB2654-CDS-F","CACCATGGCTACCG TTACCAATTT CAA","for truncated EMB2654 cloning"]
["EMB2654-CDS-11M-R","TTATGACATCCTACATGAACCGCT","for truncated EMB2654 cloning"]
["EMB976-CDS-F","CACCATGAAGAGTGACTTCCTCACTTC","for truncated EMB976 cloning"]
["EMB976-CDS-7M-R","TTAATGAACTTGAGACATTGCCAG","for truncated EMB976 cloning"]
["EMB976-CDS-14M-R","TTAATTCACAAGTATGCTAATGGT","for truncated EMB976 cloning"]
["En-EMB2654-F","TAC AATGCTTCAG AAAAGTGGGG","En-EMB2654 RT-PCR&qRT-PCR"]
["En-EMB2654-R","ATCATTGCTTCAACTTTACCACTC","En-EMB2654 RT-PCR"]
["EMB2654-F","GGTCGTCAGTATTCAAAGGCGCT","EMB2654 RT-PCR&qRT-PCR"]
["EMB2654-R","CATTTGGCTTTCTCCTTTCCTTC","EMB2654 RT-PCR"]
["En-EMB2654-qR","ATACCAGCACCACTCATCCATT","En-EMB2654 qRT-PCR"]
["EMB2654-qR","TCAGCTCTCTTCTCCCTCATGG","EMB2654 qRT-PCR"]
["En-EMB976-F","ACC TGGAACGCCT AGTTACAACA","En-EMB976 RT-PCR&qRT-PCR"]
["En-EMB976-R","TTATACATCTTGTTCTTTACCAAC","En-EMB976  RT-PCR"]
["EMB976-F","GTATGGGAAACATGTGGTGGCTG","EMB976 RT-PCR"]
["EMB976-R","TCCTCCACCATTTCTAACCACAA","EMB976  RT-PCR&qRT-PCR"]
["En-EMB976-qR","TTATCGTTTTCTCTGCTTCTGC","En-EMB976 qRT-PCR"]
["EMB976-qF","TGTACTTATGCTAGATGGGGAC","EMB976 qRT-PCR"]
["ACTIN2-qF ","TTGAATCTCCGGCGACTTGA ","for q-RT-PCR"]
["ACTIN2-qR ","CCAACATATGCATCCTTCTG ","for q-RT-PCR"]
["ACTIN2-F ","TCTTCTTCCGCTCTTTCTTTCC ","for RT-PCR"]
["ACTIN2-R ","TCTTACAATTTCCCGCTCTGC ","for RT-PCR"]
["ycf3-intron1-F ","TGTGGTAAAAAGGGGTTTCG ","for RT-PCR"]
["ycf3-intron1-R ","CGAGTCATTCCGACAACTTC ","for RT-PCR"]
["ycf3-intron2-F ","TTCGGGCATTAGAACGAAAC ","for RT-PCR"]
["ycf3-intron2-R ","TCCAATACTCAGCGGCTTG ","for RT-PCR"]
["clpP1-intron1-F ","ATGCCTATTGGCGTTCCAA ","for RT-PCR"]
["clpP1-intron1-R ","TTATTGAACCGCTACAAGATC ","for RT-PCR"]
["clpP1-intron2-F ","TAGGCCAAGAGGTTGATACC ","for RT-PCR"]
["clpP1-intron2-R ","GACTAGGATAAAGGATGCTATTG ","for RT-PCR"]
["rpl2-R ","CGGACCTCTCCAGAAGGTAAT ","for RT-PCR"]
["rpl2-F ","TTCTGGTACAGAAGTTCC ","for RT-PCR"]
["rpoC1-RT-F ","CGTCTTCCTAGTTATATTGC ","for RT-PCR"]
["rpoC1-RT-R ","TAATTGTTCTCGGATAGCAC ","for RT-PCR"]
["ndhA-F ","TTAGGTGGTCTGCGAGCTG ","for RT-PCR"]
["ndhA-R ","TTGACGCCACAAATTCCAT ","for RT-PCR"]
["rps12-F ","CGTAAAGTTGCCAGAGTACGA ","for RT-PCR"]
["rps12-R ","TTTGGCTTTTTGACCCCATA ","for RT-PCR"]
["ndhB-F ","TCATCAATGGACTCCTGACG ","for RT-PCR"]
["ndhB-R ","CCAGAAGAAGATGCCATTCA ","for RT-PCR"]
["atpF-F ","GCTCCTTCACGCAGTTCTTC ","for RT-PCR"]
["atpF-R ","TACTTGGGTCACTGGCCATC ","for RT-PCR"]
["rpl2-qF ","AAATGGGAAATGCCCTACCT ","for q-RT-PCR"]
["rpl2-qR ","CGGACCTCTCCAGAAGGTAAT ","for q-RT-PCR"]
["rpl2-intron-F ","GCCGTATGCTTTGGAAGAAG ","for q-RT-PCR"]
["atpF-qR ","GCTCCTTCACGCAGTTCTTC ","for q-RT-PCR"]
["atpF-qF ","TACTTGGGTCACTGGCCATC ","for q-RT-PCR"]
["atpF-intron-F ","TTCGGGAAGGGATCATAGAA ","for q-RT-PCR"]
["ycf3-qF2 ","TTCGGGCATTAGAACGAAAC ","for q-RT-PCR"]
["ycf3-qR2 ","TCCAATACTCAGCGGCTTG ","for q-RT-PCR"]
["ycf3-intron2-F ","AGTTGGTTGTCGAGCCGTAT ","for q-RT-PCR"]
["ycf3-qF1 ","TGTGGTAAAAAGGGGTTTCG ","for q-RT-PCR"]
["ycf3-qR1 ","CGAGTCATTCCGACAACTTC ","for q-RT-PCR"]
["ycf3-intron1-R ","TTCGTTAAAGCGAATGACGA ","for q-RT-PCR"]
["rps12-qF1 ","TCTCACACCGGGTAAATCCT ","for q-RT-PCR"]
["rps12-qR1 ","ATCCGAAACGTCACGAAATC ","for q-RT-PCR"]
["rps12-intron1-R ","GGAGCCGTATGAGGTGAAAA ","for q-RT-PCR"]
["clpP1-qF2 ","GTCGGAGGAGCAATTACCAA ","for q-RT-PCR"]
["clpP1-qR2 ","GTGATGGTTTCGCGAAGTTT ","for q-RT-PCR"]
["clpP-intron2-F ","TCATTCTGCGAAATAGAAAAACC ","for q-RT-PCR"]
["pRPL4-CDS-F","CACCATGGCTTCTTCTGCAACAGCACC","for gPRPL4 cloning"]
["PRPL4-R","cgggtgctactcattcTTAAGC","for gPRPL4 cloning"]
["PRPL4-F","ATCGAGTAGGAACATTGGAAC","for RT-PCR"]
["PRPL4-R","cgggtgctactcattcTTAAGC","for RT-PCR"]
["pRPL4-RT-R-intron span","TTAAGCTTCCTCTGACCCTTCC","for RT-qPCR"]