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[]
["Analysis Type","Singleplex"]
["Endogenous Control","GAPDH"]
["RQ Min/Max Confidence Level","95.0"]
["Reference Sample","HCT116-CON"]

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["Block Type","384-Well Block"]
["Calibration Background is expired ","No"]
["Calibration Background performed on","11-09-2021"]
["Calibration Pure Dye ABY is expired","No"]
["Calibration Pure Dye ABY performed on","11-09-2021"]
["Calibration Pure Dye CY5 is expired","No"]
["Calibration Pure Dye CY5 performed on","11-09-2021"]
["Calibration Pure Dye FAM is expired","No"]
["Calibration Pure Dye FAM performed on","11-09-2021"]
["Calibration Pure Dye JUN is expired","No"]
["Calibration Pure Dye JUN performed on","11-09-2021"]
["Calibration Pure Dye MUSTANG PURPLE is expired","No"]
["Calibration Pure Dye MUSTANG PURPLE performed on","11-09-2021"]
["Calibration Pure Dye NED is expired","No"]
["Calibration Pure Dye NED performed on","11-09-2021"]
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["Calibration Pure Dye ROX performed on","11-09-2021"]
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["Calibration Pure Dye SYBR performed on","11-09-2021"]
["Calibration Pure Dye TAMRA is expired","No"]
["Calibration Pure Dye TAMRA performed on","11-09-2021"]
["Calibration Pure Dye VIC is expired","No"]
["Calibration Pure Dye VIC performed on","11-09-2021"]
["Calibration ROI is expired ","No"]
["Calibration ROI performed on","11-09-2021"]
["Calibration Uniformity is expired ","No"]
["Calibration Uniformity performed on","11-09-2021"]
["Chemistry","SYBR_GREEN"]
["Date Created","2023-02-15 00:32:49 AM CST"]
["Experiment Barcode"]
["Experiment Comment"]
["Experiment File Name","D:\\ZSJ\\2023-02-14_221811.eds"]
["Experiment Name","2023-02-14_221811"]
["Experiment Run End Time","2023-02-15 00:02:54 AM CST"]
["Experiment Type","Comparative Cт (ΔΔCт) "]
["Instrument Name","      272530754"]
["Instrument Serial Number","272530754"]
["Instrument Type","QuantStudio™ 5 System"]
["Passive Reference","ROX"]
["Post-read Stage/Step",""]
["Pre-read Stage/Step",""]
["Quantification Cycle Method","Ct"]
["Signal Smoothing On","true"]
["Stage where Melt Analysis is performed","Stage3"]
["Stage/ Cycle where Ct Analysis is performed","Stage2, Step2"]
["User Name"]
[]
["Well","Well Position","Reading","Temperature","Fluorescence","Derivative","Target Name"]
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{"sheet":"Melt Curve Result","index":4,"n_rows":84,"n_cols":6,"rows_kept":84,"flags":["formulas_cached_only"]}
["Block Type","384-Well Block"]
["Calibration Background is expired ","No"]
["Calibration Background performed on","11-09-2021"]
["Calibration Pure Dye ABY is expired","No"]
["Calibration Pure Dye ABY performed on","11-09-2021"]
["Calibration Pure Dye CY5 is expired","No"]
["Calibration Pure Dye CY5 performed on","11-09-2021"]
["Calibration Pure Dye FAM is expired","No"]
["Calibration Pure Dye FAM performed on","11-09-2021"]
["Calibration Pure Dye JUN is expired","No"]
["Calibration Pure Dye JUN performed on","11-09-2021"]
["Calibration Pure Dye MUSTANG PURPLE is expired","No"]
["Calibration Pure Dye MUSTANG PURPLE performed on","11-09-2021"]
["Calibration Pure Dye NED is expired","No"]
["Calibration Pure Dye NED performed on","11-09-2021"]
["Calibration Pure Dye ROX is expired","No"]
["Calibration Pure Dye ROX performed on","11-09-2021"]
["Calibration Pure Dye SYBR is expired","No"]
["Calibration Pure Dye SYBR performed on","11-09-2021"]
["Calibration Pure Dye TAMRA is expired","No"]
["Calibration Pure Dye TAMRA performed on","11-09-2021"]
["Calibration Pure Dye VIC is expired","No"]
["Calibration Pure Dye VIC performed on","11-09-2021"]
["Calibration ROI is expired ","No"]
["Calibration ROI performed on","11-09-2021"]
["Calibration Uniformity is expired ","No"]
["Calibration Uniformity performed on","11-09-2021"]
["Chemistry","SYBR_GREEN"]
["Date Created","2023-02-15 00:32:49 AM CST"]
["Experiment Barcode"]
["Experiment Comment"]
["Experiment File Name","D:\\ZSJ\\2023-02-14_221811.eds"]
["Experiment Name","2023-02-14_221811"]
["Experiment Run End Time","2023-02-15 00:02:54 AM CST"]
["Experiment Type","Comparative Cт (ΔΔCт) "]
["Instrument Name","      272530754"]
["Instrument Serial Number","272530754"]
["Instrument Type","QuantStudio™ 5 System"]
["Passive Reference","ROX"]
["Post-read Stage/Step",""]
["Pre-read Stage/Step",""]
["Quantification Cycle Method","Ct"]
["Signal Smoothing On","true"]
["Stage where Melt Analysis is performed","Stage3"]
["Stage/ Cycle where Ct Analysis is performed","Stage2, Step2"]
["User Name"]
[]
["Well","Well Position","Sample","Target Name","Tm","Melt Peak Height"]
[1,"A1","HT-29-CON","GAPDH",{"v":85.92892456054688,"d":"85.929"},{"v":56552.41796875,"d":"56,552.418"}]
[2,"A2","HT-29-CON","GAPDH",{"v":86.0047378540039,"d":"86.005"},{"v":54398.02734375,"d":"54,398.027"}]
[3,"A3","HT-29-CON","GAPDH",{"v":86.0047378540039,"d":"86.005"},{"v":67467.3515625,"d":"67,467.352"}]
[4,"A4","HT-29-SI","GAPDH",{"v":86.08055114746094,"d":"86.081"},{"v":55108.73828125,"d":"55,108.738"}]
[5,"A5","HT-29-SI","GAPDH",{"v":86.154296875,"d":"86.154"},{"v":61719.640625,"d":"61,719.641"}]
[6,"A6","HT-29-SI","GAPDH",{"v":86.154296875,"d":"86.154"},{"v":63381.19140625,"d":"63,381.191"}]
[25,"B1","HT-29-CON","CBX2",{"v":86.53543090820312,"d":"86.535"},{"v":46323.6328125,"d":"46,323.633"}]
[26,"B2","HT-29-CON","CBX2",{"v":86.53543090820312,"d":"86.535"},{"v":41217.30078125,"d":"41,217.301"}]
[27,"B3","HT-29-CON","CBX2",{"v":86.68706512451172,"d":"86.687"},{"v":48328.31640625,"d":"48,328.316"}]
[28,"B4","HT-29-SI","CBX2",{"v":86.68706512451172,"d":"86.687"},{"v":53288.41796875,"d":"53,288.418"}]
[29,"B5","HT-29-SI","CBX2",{"v":86.76074981689453,"d":"86.761"},{"v":60506.70703125,"d":"60,506.707"}]
[30,"B6","HT-29-SI","CBX2",{"v":86.76074981689453,"d":"86.761"},{"v":51803.828125,"d":"51,803.828"}]
[49,"C1","HCT116-CON","GAPDH",{"v":86.08055114746094,"d":"86.081"},{"v":40435.19921875,"d":"40,435.199"}]
[50,"C2","HCT116-CON","GAPDH",{"v":86.15636444091797,"d":"86.156"},{"v":46950.58984375,"d":"46,950.590"}]
[51,"C3","HCT116-CON","GAPDH",{"v":86.15636444091797,"d":"86.156"},{"v":51540.265625,"d":"51,540.266"}]
[52,"C4","HCT116-PcDNA","GAPDH",{"v":86.232177734375,"d":"86.232"},{"v":60760.0234375,"d":"60,760.023"}]
[53,"C5","HCT116-PcDNA","GAPDH",{"v":86.2301025390625,"d":"86.230"},{"v":52718.203125,"d":"52,718.203"}]
[54,"C6","HCT116-PcDNA","GAPDH",{"v":86.305908203125,"d":"86.306"},{"v":54682.6640625,"d":"54,682.664"}]
[73,"D1","HCT116-CON","EGR4",{"v":90.25032043457031,"d":"90.250"},{"v":36475.22265625,"d":"36,475.223"}]
[74,"D2","HCT116-CON","EGR4",{"v":90.25032043457031,"d":"90.250"},{"v":35883.453125,"d":"35,883.453"}]
[75,"D3","HCT116-CON","EGR4",{"v":90.4019546508789,"d":"90.402"},{"v":29650.96875,"d":"29,650.969"}]
[76,"D4","HCT116-PcDNA","EGR4",{"v":90.55358123779297,"d":"90.554"},{"v":52234.046875,"d":"52,234.047"}]
[77,"D5","HCT116-PcDNA","EGR4",{"v":90.55110168457031,"d":"90.551"},{"v":58279.03125,"d":"58,279.031"}]
[78,"D6","HCT116-PcDNA","EGR4",{"v":90.62690734863281,"d":"90.627"},{"v":53105.8515625,"d":"53,105.852"}]
[97,"E1","SW620-CON","GAPDH",{"v":85.92892456054688,"d":"85.929"},{"v":65701.2109375,"d":"65,701.211"}]
[98,"E2","SW620-CON","GAPDH",{"v":85.92892456054688,"d":"85.929"},{"v":57430.76171875,"d":"57,430.762"}]
[99,"E3","SW620-CON","GAPDH",{"v":86.0047378540039,"d":"86.005"},{"v":65073.578125,"d":"65,073.578"}]
[100,"E4","SW620-PcDNA","GAPDH",{"v":86.0047378540039,"d":"86.005"},{"v":48700.171875,"d":"48,700.172"}]
[101,"E5","SW620-PcDNA","GAPDH",{"v":86.00267791748047,"d":"86.003"},{"v":54966.50390625,"d":"54,966.504"}]
[102,"E6","SW620-PcDNA","GAPDH",{"v":86.0784912109375,"d":"86.078"},{"v":56250.05859375,"d":"56,250.059"}]
[121,"F1","SW620-CON","EGR4",{"v":90.25032043457031,"d":"90.250"},{"v":51681.3203125,"d":"51,681.320"}]
[122,"F2","SW620-CON","EGR4",{"v":90.32614135742188,"d":"90.326"},{"v":53394.71875,"d":"53,394.719"}]
[123,"F3","SW620-CON","EGR4",{"v":90.4019546508789,"d":"90.402"},{"v":49683.6171875,"d":"49,683.617"}]
[124,"F4","SW620-PcDNA","EGR4",{"v":90.62939453125,"d":"90.629"},{"v":78790.046875,"d":"78,790.047"}]
[125,"F5","SW620-PcDNA","EGR4",{"v":90.55110168457031,"d":"90.551"},{"v":58771.3515625,"d":"58,771.352"}]
[126,"F6","SW620-PcDNA","EGR4",{"v":90.62690734863281,"d":"90.627"},{"v":52181.890625,"d":"52,181.891"}]