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{"sheet":"Sheet1","index":0,"n_rows":39,"n_cols":6,"rows_kept":39,"merged":[[0,1,0,2],[1,1,9,1],[10,1,13,1],[14,1,16,1],[19,1,19,2],[20,1,20,2],[22,1,22,2],[23,1,23,2],[25,1,25,2],[26,1,26,2],[27,1,27,2],[28,1,28,2],[29,1,29,2],[30,1,30,2],[31,1,31,2]],"flags":["formulas_cached_only"]}
[null,"Items or reagents",null,"cost/sample(￥)"]
[null,"Cell processing","Lysozyme",0.005192]
[null,null,"RNase Inhibitor",31.5]
[null,null,"dNTPs",14.375]
[null,null,"Maxima H Minus Reverse Transcriptase",555.975]
[null,null,"PEG 8000",9.15]
[null,null,"proteinase K",2.256]
[null,null,"DNaseI",46.68]
[null,null,"T4 ligase",400]
[null,null,"primer",55.34375]
[null,"rRNA removal","TSO (100μM) and rRNA-tRNA probe",5]
[null,null,"Streptavidin magnetic beads",23.12]
[null,null,"probe primer","~0"]
[null,null,"biotin primer",5]
[null,"library construction","VAHTS DNA Clean Beads",16]
[null,null,"KAPA HIFI hotStart ReadyMix PCR Kits",14.72]
[null,null,"VAHTS Universal DNA Library Prep Kit",112.5]
[null,"Sequencing","Sequencing costs",1250]
[]
[null,"The total cost in RMB",null,"cost/sample(￥)"]
[null,"total (￥)",null,2541.6249419999995]
[]
[null,"￥ convert to $",null,"cost/sample($)"]
[null,"total ($)",null,347.2165221311474]
[]
[null,"Number of cells sequenced",null,"Cost per cell"]
[null,20000,null,0.01736082610655737]
[null,30000,null,0.011573884071038248]
[null,40000,null,0.008680413053278685]
[null,50000,null,0.006944330442622948]
[null,60000,null,0.005786942035519124]
[null,70000,null,0.004960236030444963]
[]
[null,null,"Changes in the number of cells detected with increasing reads during sequencing"]
[]
[]
[]
[{"$nulls":5},"Sequenced about 70,000 cells."]
[{"$nulls":5},"$0.0049/celll"]