{"text_format":"3","workbook":"xls","n_sheets":4,"sheets":["Metadata Template","Matrix non-normalized Template","Matrix normalized Template","Fold Change Template"],"not_rendered":{"external_links":1,"comments":28},"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Metadata Template","index":0,"n_rows":141,"n_cols":14,"rows_kept":141,"merged":[[10,1,10,5],[11,1,11,11],[12,1,12,11],[13,1,13,11],[117,1,117,11],[118,1,118,11],[119,1,119,11]],"flags":["formulas_cached_only"]}
["# Use this template for large-scale, high-throughput RT-PCR submissions."]
["# In addition to this 'Metadata Template' worksheet, there are related 'Matrix non-normalized', 'Matrix normalized', and 'Fold Change' worksheets. Click tabs below."]
["# Most fields in this template must be completed. Incomplete submissions will be returned."]
["# For 'Example' worksheets, click tabs below."]
["# Field names (in blue on this page) should not be edited. Hover over cells containing field names to view field content guidelines or, "]
["# CLICK HERE for Field Content Guidelines Web page."]
[]
["SERIES"]
["# This section describes the overall experiment."]
[]
["title","Real-time quantitative PCR analysis of PARP1 in BCR-ABL p190+ leukemia patients"]
["summary","PARP1 expression was quantified from the buff coat of acute lymphoblastic leukemia and chronic myeloid leukemia patients"]
["summary"]
["overall design","Total RNA was extracted from the buff coat in the peripheral blood of ALL and CML BCR-ABL p190+ patients, totalizing 60 patients, covnerted into cDNA and quantified, being compared to a sample of 10 healthy blood donors"]
["contributor","Caio, B, Machado"]
["contributor","Emerson, L, Silva"]
["contributor","Wallax, A, S, Ferreira"]
["contributor","Flávia, M, C, P, Pessoa"]
["contributor","Andreza, U, Quadros"]
["contributor","Daianne, M, C, Fantacini"]
["contributor","Izadora, P, Furtado"]
["contributor","Rafaela, Rossetti"]
["contributor","Roberta, M, Silveira"]
["contributor","Sarah, C, G, Lima"]
["contributor","Fernando, A, R, Mello-Júnior"]
["contributor","Aline, D, Seabra"]
["contributor","Edith, C, O, Moreira"]
["contributor","Manoel, O, Moraes-Filho"]
["contributor","Maria, E, A, Moraes"]
["contributor","Raquel, C, Montenegro"]
["contributor","Rodrigo, M, Ribeiro"]
["contributor","André, S, Khayat"]
["contributor","Rommel, M, R, Burbano"]
["contributor","Edivaldo, H, C, Oliveira"]
["contributor","Dimas, T, Covas"]
["contributor","Lucas, E, B, Souza"]
["contributor","Caroline, A, Moreira-Nunes"]
[]
["SAMPLES"]
["# The Sample names in the first column are arbitrary but they must match the column headers of the Matrix table (see next worksheet)."]
[]
["Sample name","title","description","source name","organism","characteristics: Diagnostic","characteristics: Gender","characteristics: Age","characteristics: Immunophenotype","characteristics: Karyotype","characteristics: WBC count per mililiter","characteristics: Blast % in peripheral blood","molecule","label"]
["SAMPLE 1","Healthy Blood Donor 1","Control","Buff coat of peripheral blood","Homo sapiens","Healthy","Female",29,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 2","Healthy Blood Donor 2","Control","Buff coat of peripheral blood","Homo sapiens","Healthy","Male",33,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 3","Healthy Blood Donor 3","Control","Buff coat of peripheral blood","Homo sapiens","Healthy","Female",32,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 4","Healthy Blood Donor 4","Control","Buff coat of peripheral blood","Homo sapiens","Healthy","Male",20,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 5","Healthy Blood Donor 5","Control","Buff coat of peripheral blood","Homo sapiens","Healthy","Female",19,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 6","Healthy Blood Donor 6","Control","Buff coat of peripheral blood","Homo sapiens","Healthy","Female",36,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 7","Healthy Blood Donor 7","Control","Buff coat of peripheral blood","Homo sapiens","Healthy","Female",36,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 8","Healthy Blood Donor 8","Control","Buff coat of peripheral blood","Homo sapiens","Healthy","Female",26,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 9","Healthy Blood Donor 9","Control","Buff coat of peripheral blood","Homo sapiens","Healthy","Female",24,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 10","Healthy Blood Donor 10","Control","Buff coat of peripheral blood","Homo sapiens","Healthy","Female",33,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 11","ALL Patient 1","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",35,"Positive for: CD10, CD19, CD22, CD33, CD34, CD38, CD45, CD79A and CD123","46, XY, t(9;22)(q34;q11.2) [11] / 56, XY, +X, +2, +6, +13, +14, +17, +21, +21, +der(22), t(9;22) [8] / 46,XY [1] ",{"v":9600,"d":"9,600"},58,"Total RNA","FAM"]
["SAMPLE 12","ALL Patient 2","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",54,"Positive for: CD10, CD13, CD19, CD22, CD33, CD38, CD45, CD58, CD79A and CD123","-",{"v":25200,"d":"25,200"},"-","Total RNA","FAM"]
["SAMPLE 13","ALL Patient 3","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",24,"Positive for: CD10, CD19, CD38, CD73, CD81 and CD304","51,XY, +X, trp(1)(q21q32), inv(5)(q13q35), del(6)(q23), +7, +8, der(19)t(1;19)(q23;p13.3), +20, +21 [18] / 46, XY [2] ",{"v":4900,"d":"4,900"},25,"Total RNA","FAM"]
["SAMPLE 14","ALL Patient 4","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Female",36,"Positive for: CD10, CD19, CD22, CD33, CD34, CD38, CD45, CD58, CD79A, CD81","46,XX, t(9;22)(q34;q11.2), -13, add(14(q32), del(20)(q13.1), +mar [17] / 46,XX, +11, der(11;18)(q10;q10) [3]",{"v":12400,"d":"12,400"},"-","Total RNA","FAM"]
["SAMPLE 15","ALL Patient 5","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",29,"Positive for: CD10, CD19, CD20, CD22, CD33, CD34, CD38, CD45, CD58, CD81, CD79A","64,XXY, -3,-7, del(7)(p15), -8, t(9;22)(q34;q11.20), -11,-12, del(12)(p12), -17, -19, +21, der(22)t(9;22), +mar [5] / 64,XXY, der(9)t(9;22)(q34;q11.2)add(9)(p24) [5] / 46,XY [10]",{"v":6600,"d":"6,600"},65,"Total RNA","FAM"]
["SAMPLE 16","ALL Patient 6","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",69,"Positive for: CD10, CD19, CD20, CD22, CD38, CD58, CD79A, CD81, IgM","-",{"v":3300,"d":"3,300"},"-","Total RNA","FAM"]
["SAMPLE 17","ALL Patient 7","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",90,"Positive for: CD19, CD20, CD22, CD38, CD45, cyCD79a, CD81, IgM.","-",{"v":224500,"d":"224,500"},79,"Total RNA","FAM"]
["SAMPLE 18","ALL Patient 8","Test","Buff coat of peripheral blood","Homo sapiens","ALL","Female",33,"-","46, XX [20]",{"v":12200,"d":"12,200"},"-","Total RNA","FAM"]
["SAMPLE 19","ALL Patient 9","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",55,"Positive for: CD10, CD19, CD20, CD38 and CD45\nNegative for: CD13, CD33 and CD34","-",{"v":240610,"d":"240,610"},87,"Total RNA","FAM"]
["SAMPLE 20","ALL Patient 10","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",35,"Positive for: CD3, CD7, CD10, CD19, CD34, CD38, CD45 and CD79A\nNegative for: CD2, CD5, CD13, CD22, CD33 and MPO","46, XY [18] / 46, XY t(9;22) [2]",{"v":93100,"d":"93,100"},50,"Total RNA","FAM"]
["SAMPLE 21","ALL Patient 11","Test","Buff coat of peripheral blood","Homo sapiens","ALL","Female",31,"-","46, XX t(9;22) [10] / 46, XX t(9;22), t(12;21) [4] / 48, XX, +4, t(9;22), t(12;21), +21 [2] / 50, XX, +4, t(9;22), t(12;21), +14, +18, +21 [4]",{"v":65410,"d":"65,410"},91,"Total RNA","FAM"]
["SAMPLE 22","ALL Patient 12","Test","Buff coat of peripheral blood","Homo sapiens","ALL","Male",72,"-","46, XY [20]",{"v":43670,"d":"43,670"},36,"Total RNA","FAM"]
["SAMPLE 23","ALL Patient 13","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",34,"Positive for: CD10, CD19, CD34, CD38, CD79A and HLA-DR\nNegative for: CD3, CD7, CD13, CD20, CD22, CD33, CD45, CD117, MPO and NG2","46, XY t(9;22) [16] / 46, XY, del(7q), t(9;22) [4]",{"v":7600,"d":"7,600"},53,"Total RNA","FAM"]
["SAMPLE 24","ALL Patient 14","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",52,"Positive for: CD10, CD19, CD22, CD33, CD34, CD79A, HLA-DR and IGM\nNegative for: CD3, CD7, CD13, CD20, CD38, CD117, MPO and NG2","-",{"v":107930,"d":"107,930"},72,"Total RNA","FAM"]
["SAMPLE 25","ALL Patient 15","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",52,"Positive for: CD10, CD19, CD34, CD38 and CD45\nNegative for: CD13, CD20, CD33 and CD58","-",{"v":2630,"d":"2,630"},"-","Total RNA","FAM"]
["SAMPLE 26","ALL Patient 16","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Female",21,"Positive for: CD10, CD19, CD20, CD22, CD34, CD38, CD45, CD79A and HLA-DR\nNegative for: CD3, CD7, CD13, CD33, CD117, IGM, MPO and NG2"," 46, X,-X,t(9;22),+21 [20]",{"v":25290,"d":"25,290"},63,"Total RNA","FAM"]
["SAMPLE 27","ALL Patient 17","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Female",47,"Positive for: CD10, CD19, CD20, CD34 and CD45\nNegative for: CD13, CD33, CD38 and CD58","47, XX, t(9;22), + der(22) [20]",250,"-","Total RNA","FAM"]
["SAMPLE 28","ALL Patient 18","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",26,"Positive for: CD10, CD19, CD22, CD34, CD79A and HLA-DR\nNegative for: CD3, CD7, CD13, CD20, CD33, CD38, CD45, CD58, IGM, MPO and NG2","46, XY [15] / 47, XY,t(9;22),+21 [5]",{"v":6720,"d":"6,720"},96,"Total RNA","FAM"]
["SAMPLE 29","ALL Patient 19","Test","Buff coat of peripheral blood","Homo sapiens","ALL","Female",31,"Positive for: CD13, CD33, CD34, CD117 and HLA-DR\nNegative for: CD2, CD3, CD4, CD7, CD8, CD11B, CD11C, CD14, CD15, CD16, CD56 and CD64"," 46, XX, del(6q), t(9;22) [20]",{"v":1530,"d":"1,530"},"-","Total RNA","FAM"]
["SAMPLE 30","ALL Patient 20","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",47,"Positive for: CD10, CD19, CD20, CD22, CD34, CD38, CD79A and HLA-DR\nNegative for: CD3, CD7, CD13, CD33, CD34, CD58, CD117, IGM, MPO and NG2","46, XY [9] / 46, XY t(9;22) [11]",{"v":7610,"d":"7,610"},2.5,"Total RNA","FAM"]
["SAMPLE 31","ALL Patient 21","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",45,"Positive for: CD10, CD19, CD34, CD38 and CD45\nNegative for: CD13, CD20, CD33 and CD58","46, XY [20]",{"v":3880,"d":"3,880"},2,"Total RNA","FAM"]
["SAMPLE 32","ALL Patient 22","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Female",58,"Positive for: CD7, CD10, CD19, CD34, CD38, CD45, CD58, CD79A and HLA-DR\nNegative for: CD3, CD13, CD20, CD22, CD33, CD117, IGM, MPO and NG2","-",{"v":139560,"d":"139,560"},87,"Total RNA","FAM"]
["SAMPLE 33","ALL Patient 23","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",20,"Positive for: CD10, CD19, CD34, CD38 and CD45\nNegative for: CD13, CD20, CD33 and CD58","-",{"v":1640,"d":"1,640"},"-","Total RNA","FAM"]
["SAMPLE 34","ALL Patient 24","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",32,"Positive for: CD10, CD13, CD19, CD33, CD34 and CD45\nNegative for: CD20, C38 and CD58","46, XY [19] / 46, XY t(9;22) [1]",{"v":82920,"d":"82,920"},84,"Total RNA","FAM"]
["SAMPLE 35","ALL Patient 25","Test","Buff coat of peripheral blood","Homo sapiens","ALL","Male",20,"-","-","1.690(10/02/2021)","-","Total RNA","FAM"]
["SAMPLE 36","ALL Patient 26","Test","Buff coat of peripheral blood","Homo sapiens","ALL","Male",22,"-","-",{"v":11590,"d":"11,590"},"-","Total RNA","FAM"]
["SAMPLE 37","ALL Patient 27","Test","Buff coat of peripheral blood","Homo sapiens","ALL","Female",30,"-","-",920,"-","Total RNA","FAM"]
["SAMPLE 38","ALL Patient 28","Test","Buff coat of peripheral blood","Homo sapiens","ALL","Male",38,"-","48, XY, +8, t(9;22), +21 [20]",{"v":533240,"d":"533,240"},11,"Total RNA","FAM"]
["SAMPLE 39","ALL Patient 29","Test","Buff coat of peripheral blood","Homo sapiens","ALL","Male",33,"-","-",{"v":69020,"d":"69,020"},80,"Total RNA","FAM"]
["SAMPLE 40","ALL Patient 30","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Male",21,"Positive for: CD10, CD19, CD33, CD34, CD38, CD45 and CD58\nNegative for: CD13 and CD20","-",{"v":4490,"d":"4,490"},"-","Total RNA","FAM"]
["SAMPLE 41","ALL Patient 31","Test","Buff coat of peripheral blood","Homo sapiens","B-ALL","Female",56,"Positive for: CD10, CD19, CD22, CD34, CD38, CD79A, HLA-DR and TDT\nNegative for: CD3, CD7, CD13, CD20, CD33, CD45, CD58, CD117, IGM, MPO and NG2","-",{"v":102000,"d":"102,000"},83,"Total RNA","FAM"]
["SAMPLE 42","CML Patient 1","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",49,"-","-",{"v":6080,"d":"6,080"},"-","Total RNA","FAM"]
["SAMPLE 43","CML Patient 2","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",34,"-","-","5.760 (12/12/2022)","-","Total RNA","FAM"]
["SAMPLE 44","CML Patient 3","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",63,"-","-",{"v":13760,"d":"13,760"},"-","Total RNA","FAM"]
["SAMPLE 45","CML Patient 4","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",50,"-","-","8140 (01/09/2020)","-","Total RNA","FAM"]
["SAMPLE 46","CML Patient 5","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",67,"-","-","9.970(13/09/2020)","-","Total RNA","FAM"]
["SAMPLE 47","CML Patient 6","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",81,"-","-","7.360 (07/0/2020)","-","Total RNA","FAM"]
["SAMPLE 48","CML Patient 7","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",81,"-","-","10.500 (16/11/2020)","-","Total RNA","FAM"]
["SAMPLE 49","CML Patient 8","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",41,"-","46, XX t(9;22) [20]",{"v":225510,"d":"225,510"},2.5,"Total RNA","FAM"]
["SAMPLE 50","CML Patient 9","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",45,"-","-",{"v":234820,"d":"234,820"},"-","Total RNA","FAM"]
["SAMPLE 51","CML Patient 10","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",39,"-","-",{"v":4000,"d":"4,000"},2.5,"Total RNA","FAM"]
["SAMPLE 52","CML Patient 11","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",61,"-","-",{"v":14600,"d":"14,600"},"-","Total RNA","FAM"]
["SAMPLE 53","CML Patient 12","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",62,"-","-",{"v":3750,"d":"3,750"},"-","Total RNA","FAM"]
["SAMPLE 54","CML Patient 13","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",49,"-","-",{"v":6320,"d":"6,320"},"-","Total RNA","FAM"]
["SAMPLE 55","CML Patient 14","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",51,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 56","CML Patient 15","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",90,"-","-",{"v":3870,"d":"3,870"},"-","Total RNA","FAM"]
["SAMPLE 57","CML Patient 16","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",73,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 58","CML Patient 17","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",58,"-","-",{"v":4150,"d":"4,150"},"-","Total RNA","FAM"]
["SAMPLE 59","CML Patient 18","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",72,"-","-","6.850(01/09/2021)","-","Total RNA","FAM"]
["SAMPLE 60","CML Patient 19","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",56,"-","-",{"v":7150,"d":"7,150"},"-","Total RNA","FAM"]
["SAMPLE 61","CML Patient 20","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",56,"-","-","15.400(24/08/2021)","-","Total RNA","FAM"]
["SAMPLE 62","CML Patient 21","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",52,"-","-","-","-","Total RNA","FAM"]
["SAMPLE 63","CML Patient 22","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",54,"-","-",{"v":5100,"d":"5,100"},"-","Total RNA","FAM"]
["SAMPLE 64","CML Patient 23","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",39,"-","47, XY t(9:22), +21 [12] / 48, XY (t9:22), +17, +21 [8]",{"v":388690,"d":"388,690"},6,"Total RNA","FAM"]
["SAMPLE 65","CML Patient 24","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",67,"-","-","7.070(20/06/2022)","-","Total RNA","FAM"]
["SAMPLE 66","CML Patient 25","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",42,"-","-",{"v":3650,"d":"3,650"},"-","Total RNA","FAM"]
["SAMPLE 67","CML Patient 26","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",62,"-","-",{"v":8180,"d":"8,180"},"-","Total RNA","FAM"]
["SAMPLE 68","CML Patient 27","Test","Buff coat of peripheral blood","Homo sapiens","CML","Female",34,"Positive for: CD13, CD33, CD34, CD45, CD117 and HLA-DR\nNegative for: CD2, CD3, CD7, CD11B, CD11C, CD16, CD19, CD56 and CD64","-",{"v":195070,"d":"195,070"},"-","Total RNA","FAM"]
["SAMPLE 69","CML Patient 28","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",70,"-","-",{"v":9190,"d":"9,190"},"-","Total RNA","FAM"]
["SAMPLE 70","CML Patient 29","Test","Buff coat of peripheral blood","Homo sapiens","CML","Male",52,"-","-",{"v":9240,"d":"9,240"},"-","Total RNA","FAM"]
[]
["PROTOCOLS"]
["# This section includes protocols and fields which are common to all Samples."]
["# Protocols which are applicable to specific Samples or specific channels should be included in additional columns of the SAMPLES section instead."]
[]
["extract protocol","Total RNA was extracted with TRIzol reagent (ThermoFisher) according to manufacturer's protocol"]
["label protocol","Reverse transcription was performed from 400 ng of total RNA using the GoScript Reverse Transcription System (Promega). qPCR was perfomed in QuantStudio 5 (ThermoFisher), using the GoTaq Probe qPCR Master Mix (Promega), following the cycles recomended by the manufacturer"]
["hyb protocol","n/a"]
["scan protocol","n/a"]
["data processing","Normalization was achieved through the housekepping gene: ACTB. Target gene signal normalized to housekeeping gene; deltaCt = (Ct_PARP1 − Ct_ACTB). Average deltaCT of control group was used as a calibrator; deltadeltaCT = (delta_CT_patient - delta_CT_control). Final fold change expression determined as 2^- (deltadeltaCT)"]
["value definition","Matrix normalized worksheet reports normalized signal (against housekeeping gene). Fold Change worksheet reports test/control (patient/healthy donors) ratios, with control representing the pooled and expression of all healthy donors."]
[]
["PLATFORM"]
["# If your panel of PCR assays is already deposited in GEO, please fill in platform column in SAMPLES section and ignore this PLATFORM section."]
["# If your panel of PCR assays is not in GEO, please complete the fields below and include platform annotation columns in Matrix normalized Template (see example)."]
[]
["title","ThermoFisher custom TaqMan Gene Expression Assay (human)"]
["technology","RT-PCR"]
["distribution","commercial"]
["organism","Homo sapiens"]
["manufacturer","ThermoFisher Scientific"]
["manufacture protocol","see manufacturer's website"]
["catalog number",4331182]
["description","TaqMan-based qRT-PCR assay"]
[]
["# Platform Column Definitions: describe the contents of each platform column in the Matrix normalized Template"]
["Symbol","Gene symbol"]
["Gene Name","Gene name"]
["Assay","Primer assay"]

{"sheet":"Matrix non-normalized Template","index":1,"n_rows":7,"n_cols":71,"rows_kept":7,"flags":["formulas_cached_only"]}
["# Matrix non-normalized table for RT-PCR submission."]
["# The ID_REF column must contain unique identifiers."]
["# SAMPLE columns should report non-normalized data (e.g., raw Ct_Target)."]
["# SAMPLE column header names must match Sample name column in SAMPLES section of Metadata sheet."]
[]
["ID_REF","SAMPLE 1","SAMPLE 2","SAMPLE 3","SAMPLE 4","SAMPLE 5","SAMPLE 6","SAMPLE 7","SAMPLE 8","SAMPLE 9","SAMPLE 10","SAMPLE 11","SAMPLE 12","SAMPLE 13","SAMPLE 14","SAMPLE 15","SAMPLE 16","SAMPLE 17","SAMPLE 18","SAMPLE 19","SAMPLE 20","SAMPLE 21","SAMPLE 22","SAMPLE 23","SAMPLE 24","SAMPLE 25","SAMPLE 26","SAMPLE 27","SAMPLE 28","SAMPLE 29","SAMPLE 30","SAMPLE 31","SAMPLE 32","SAMPLE 33","SAMPLE 34","SAMPLE 35","SAMPLE 36","SAMPLE 37","SAMPLE 38","SAMPLE 39","SAMPLE 40","SAMPLE 41","SAMPLE 42","SAMPLE 43","SAMPLE 44","SAMPLE 45","SAMPLE 46","SAMPLE 47","SAMPLE 48","SAMPLE 49","SAMPLE 50","SAMPLE 51","SAMPLE 52","SAMPLE 53","SAMPLE 54","SAMPLE 55","SAMPLE 56","SAMPLE 57","SAMPLE 58","SAMPLE 59","SAMPLE 60","SAMPLE 61","SAMPLE 62","SAMPLE 63","SAMPLE 64","SAMPLE 65","SAMPLE 66","SAMPLE 67","SAMPLE 68","SAMPLE 69","SAMPLE 70"]
["A01",27.43507480621338,28.799715042114258,28.146955490112305,27.859439849853516,28.698942184448242,28.42002582550049,27.55067539215088,27.88892936706543,26.496336936950684,25.741829872131348,26.30726718902588,30.295061111450195,24.16866970062256,25.17899799346924,27.225457191467285,26.22384262084961,25.259751319885254,33.421369552612305,27.893634796142578,26.216038703918457,23.44368553161621,25.243654251098633,24.751272201538086,26.953761100769043,32.71475791931152,25.80196189880371,27.15749168395996,26.220694541931127,35.10208320617676,37.610755920410156,28.58566665649414,29.68047046661377,27.388607025146484,27.826927185058594,28.02414035797119,28.737360954284668,25.962663650512695,27.13893413543701,25.10441017150879,27.7949275970459,24.048466682434082,26.660547256469727,32.10103511810303,30.531766891479492,32.77789306640621,29.758054733276367,32.86393356323242,36.384490966796875,28.467422485351562,31.666972160339355,27.041091918945312,28.17227077484131,29.981383323669434,28.557311058044434,26.465795516967773,32.898494720458984,28.855496406555176,27.329668045043945,26.970664978027344,27.25245761871338,27.64643096923828,26.8336763381958,26.69060230255127,27.786453247070312,27.73155975341797,29.125746726989746,27.874239921569824,27.100457191467285,26.922886848449707,27.29291534423828]

{"sheet":"Matrix normalized Template","index":2,"n_rows":9,"n_cols":74,"rows_kept":9,"flags":["formulas_cached_only"]}
["# Matrix normalized table for RT-PCR submission."]
["# The ID_REF column must contain unique identifiers."]
["# The header 1, header 2, header 3 columns should include PLATFORM annotations if your panel of PCR assays is not in GEO."]
["# SAMPLE columns should report normalized data used to draw study's conclusions [target gene signal normalized to housekeeping genes; e.g., 2^-ΔCt, where -ΔCt = -(Ct_Target − Ct_HKG)]"]
["# SAMPLE values that should be disregarded may be left blank or labeled as \"null\"."]
["# SAMPLE column header names must match Sample name column in SAMPLES section of Metadata sheet."]
[]
["ID_REF","Symbol","Gene Name","Assay","SAMPLE 1","SAMPLE 2","SAMPLE 3","SAMPLE 4","SAMPLE 5","SAMPLE 6","SAMPLE 7","SAMPLE 8","SAMPLE 9","SAMPLE 10","SAMPLE 11","SAMPLE 12","SAMPLE 13","SAMPLE 14","SAMPLE 15","SAMPLE 16","SAMPLE 17","SAMPLE 18","SAMPLE 19","SAMPLE 20","SAMPLE 21","SAMPLE 22","SAMPLE 23","SAMPLE 24","SAMPLE 25","SAMPLE 26","SAMPLE 27","SAMPLE 28","SAMPLE 29","SAMPLE 30","SAMPLE 31","SAMPLE 32","SAMPLE 33","SAMPLE 34","SAMPLE 35","SAMPLE 36","SAMPLE 37","SAMPLE 38","SAMPLE 39","SAMPLE 40","SAMPLE 41","SAMPLE 42","SAMPLE 43","SAMPLE 44","SAMPLE 45","SAMPLE 46","SAMPLE 47","SAMPLE 48","SAMPLE 49","SAMPLE 50","SAMPLE 51","SAMPLE 52","SAMPLE 53","SAMPLE 54","SAMPLE 55","SAMPLE 56","SAMPLE 57","SAMPLE 58","SAMPLE 59","SAMPLE 60","SAMPLE 61","SAMPLE 62","SAMPLE 63","SAMPLE 64","SAMPLE 65","SAMPLE 66","SAMPLE 67","SAMPLE 68","SAMPLE 69","SAMPLE 70"]
["A01","PARP1","Poly(ADP-ribose) Polymerase 1","Hs00242302_m1",7.215574806213379,7.173215042114258,7.769455490112303,7.770939849853516,7.101442184448242,7.633525825500488,7.51517539215088,8.31442936706543,8.171836936950683,7.167329872131347,8.083267189025879,9.140561111450197,5.650669700622558,6.6749979934692405,8.028457191467286,7.999842620849609,4.459251319885254,11.199369552612303,7.486082077026367,6.669708251953125,4.420901298522949,6.300241470336914,4.742897033691406,6.768584251403809,11.197169303894043,6.461770057678223,7.945240020751953,6.089640617370581,11.450446128845215,7.282085418701172,8.514364242553711,7.425293922424316,8.817687034606934,7.433712005615234,10.851391792297363,7.533481597900391,7.711770057678223,9.138290405273438,5.30703067779541,9.302607536315918,5.2476606369018555,8.159757614135742,21.482577323913574,9.542753219604492,11.092291831970172,9.52079963684082,11.913396835327148,12.163838386535645,10.016438484191895,11.335152626037598,9.089550971984863,10.741623878479004,8.891355514526367,8.302011489868164,7.746581077575684,9.591350555419922,8.441868782043457,8.200965881347656,8.955379486083984,8.874168395996094,8.180084228515625,8.50012493133545,8.209738731384277,9.953019142150879,9.500104904174805,8.582929611206055,9.86190414428711,9.705355644226074,9.685701370239258,9.332711219787598]

{"sheet":"Fold Change Template","index":3,"n_rows":6,"n_cols":71,"rows_kept":6,"flags":["formulas_cached_only"]}
["# Fold change data = test/control"]
["# e.g., SAMPLEtest target gene signal normalized to housekeeping gene (2^-ΔCttest)/SAMPLEcontrol target gene signal normalized to HKG (2^-ΔCtcontrol)"]
["# 2^-ΔΔCt, where -ΔΔCt = -[ΔCttest -ΔCtcontrol]"]
[]
["ID_REF","Fold_change 1","Fold_change 2","Fold_change 3","Fold_change 4","Fold_change 5","Fold_change 6","Fold_change 7","Fold_change 8","Fold_change 9","Fold_change 10","Fold_change 11","Fold_change 12","Fold_change 13","Fold_change 14","Fold_change 15","Fold_change 16","Fold_change 17","Fold_change 18","Fold_change 19","Fold_change 20","Fold_change 21","Fold_change 22","Fold_change 23","Fold_change 24","Fold_change 25","Fold_change 26","Fold_change 27","Fold_change 28","Fold_change 29","Fold_change 30","Fold_change 31","Fold_change 32","Fold_change 33","Fold_change 34","Fold_change 35","Fold_change 36","Fold_change 37","Fold_change 38","Fold_change 39","Fold_change 40","Fold_change 41","Fold_change 42","Fold_change 43","Fold_change 44","Fold_change 45","Fold_change 46","Fold_change 47","Fold_change 48","Fold_change 49","Fold_change 50","Fold_change 51","Fold_change 52","Fold_change 53","Fold_change 54","Fold_change 55","Fold_change 56","Fold_change 57","Fold_change 58","Fold_change 59","Fold_change 60","Fold_change 61","Fold_change 62","Fold_change 63","Fold_change 64","Fold_change 65","Fold_change 66","Fold_change 67","Fold_change 68","Fold_change 69","Fold_change 70"]
["A01",1.2903099573252763,{"v":1.3287571312722228,"d":"1.33"},0.8789402418045786,0.8780363830318733,1.3965336073044492,0.9657801060960448,1.0483475525901829,0.6024289931882869,0.6650135029870259,1.3341885863013805,0.7071191754968948,0.33979378381034103,3.817485761444488,1.8768254518714602,0.7345004527537552,0.7492140479322924,8.718265606209577,0.08155532702219705,1.0697030797703098,1.8837195938773545,8.95312406524143,2.433530752946945,7.162163448650457,1.7589423823583015,0.0816798016349216,2.175764512214696,0.7781134679827209,2.8160088329741852,0.06852842537522416,1.232174904514355,0.5244685738002254,1.1157382042079127,0.4250208265787832,1.109246861371032,0.10380160702559474,1.0351292210388339,0.9147962893787924,0.3403290177592674,4.844211311535509,0.30369286946025414,5.047719840298354,0.6706048687168302,0.12198999053827264,0.2571245478756604,0.08783870801458783,0.2610671499108555,0.04971743374829507,0.04179441825824009,0.1851612346146168,0.07422967438727561,0.3520229772510624,0.1120076051968697,0.40386274182922016,0.6076367309168279,0.8929871987217489,0.24860753374034575,0.5514965227551177,0.6517210937025298,0.38633197430824495,0.40870281369144745,0.6612227409441347,0.5296706763142667,0.6477700833475171,0.1934822780741169,0.2648390145658858,0.5001257753978997,0.20609598893626352,0.22971816257203195,0.23286909741259204,0.2974215847471664]