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["symbol","name","hgncid","evidence","testmethod","testin","testsetting","pathway","confidence","proteinfullname","uniprotac","uniprotid","pmid"]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Silencing of SLC7A11 sensitized HT-1080 cells to erastin-induced death, whereas transfection of HT-1080 cells with a plasmid encoding SLC7A11 conferred protection from erastin- and sulfasalazine-induced death.","RT-qPCR, siRNA, gene transfection, cell viability","Human","HT-1080 cells","SLC7A11 :+: Cystine, Cystine :-: Ferroptosis","Validated","Cystine/glutamate transporter","Q9UPY5","XCT_HUMAN",22632970]
["GPX4","Glutathione peroxidase 4","HGNC:4556","RNAi-mediated GPX4 knockdown induces ferroptosis.","Affinity-based chemoproteomics, western blotting, and RNAi","Human, mice","HRAS mutant BJeLR-engineered tumor cells, HT-1080 cells, xenograft mouse model (athymic nude mice injected with four million HT-1080 cells)","GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",24439385]
["AKR1C1","Aldo-keto reductase family 1 member C1","HGNC:384","Up-regulated in DU-145 erastin-resistant clones. Participate in the detoxification of toxic lipid metabolites. May confer partial resistance to erastin by enhancing the detoxification of reactive aldehydes generated downstream of the oxidative destruction of the plasma membrane during ferroptosis.","RNA-seq, glutamate release assay, cell growth, flow cytometry","Human","DU-145 prostate cancer cells","AKR1C1 :-: Ferroptosis","Validated","Aldo-keto reductase family 1 member C1","Q04828","AK1C1_HUMAN",24844246]
["AKR1C2","Aldo-keto reductase family 1 member C2","HGNC:385","Up-regulated in DU-145 erastin-resistant clones. Participate in the detoxification of toxic lipid metabolites. May confer partial resistance to erastin by enhancing the detoxification of reactive aldehydes generated downstream of the oxidative destruction of the plasma membrane during ferroptosis.","RNA-seq, glutamate release assay, cell growth, flow cytometry","Human","DU-145 prostate cancer cells","AKR1C2 :-: Ferroptosis","Validated","Aldo-keto reductase family 1 member C2","P52895","AK1C2_HUMAN",24844246]
["AKR1C3","Aldo-keto reductase family 1 member C3","HGNC:386","Up-regulated in DU-145 erastin-resistant clones. Participate in the detoxification of toxic lipid metabolites. May confer partial resistance to erastin by enhancing the detoxification of reactive aldehydes generated downstream of the oxidative destruction of the plasma membrane during ferroptosis.","RNA-seq, glutamate release assay, cell growth, flow cytometry","Human","DU-145 prostate cancer cells","AKR1C3 :-: Ferroptosis","Validated","Aldo-keto reductase family 1 member C3","P42330","AK1C3_HUMAN",24844246]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Knockout of glutathione peroxidase 4 (Gpx4) causes cell death in a pathologically relevant form of ferroptosis. Knockdown renders cells more sensitive to ferroptosis-inducing agents.","Cell viability assessment, siRNA","Human, mice","Mouse embryonic fibroblast (MEF) cells (Pfa1 cells), Gpx4?/? cells and mice, HK-2 cells","GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",25402683]
["RB1","RB transcriptional corepressor 1","HGNC:9884","Rb knock-down cells exposed to sorafenib encounter ferroptosis. Lack of Rb sensitized HCC cells to the induction of ferroptosis.","RNA interference, cell viability assay, oxidative stress measurement","Human, mice","HCC cell lines Huh7 and PLC/PRF5; Balb/c nude mice received tumour xenografts derived from HCC cells.","RB1 :-: Ferroptosis","Validated","Retinoblastoma-associated protein","P06400","RB_HUMAN",25444922]
["HSPB1","Heat shock protein family B (small) member 1","HGNC:5246","Knockdown of HSF1 and HSPB1 enhances erastin-induced ferroptosis, whereas heat shock pretreatment and overexpression of HSPB1 inhibits erastin-induced ferroptosis.","RNAi, RT-qPCR, western blot, cell viability","Human, mice","HeLa and U2OS cells, human xenograft mouse tumor derived from HeLa (#CCL-2) cells.","HSPB1 :-: Ferroptosis","Validated","Heat shock protein beta-1","P04792","HSPB1_HUMAN",25728673]
["HSF1","Heat shock transcription factor 1","HGNC:5224","Knockdown of HSF1 and HSPB1 enhances erastin-induced ferroptosis, whereas heat shock pretreatment and overexpression of HSPB1 inhibits erastin-induced ferroptosis.","RNAi, RT-qPCR, western blot, cell viability","Human, mice","HeLa and U2OS cells, human xenograft mouse tumor derived from HeLa (#CCL-2) cells.","HSF1 :-: Ferroptosis","Validated","Heat shock factor protein 1","Q00613","HSF1_HUMAN",25728673]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Overexpressed in human cancer specimens. Overexpression inhibits ROS-induced ferroptosis.","Western blot, cell death test, ChIP assay, RT-PCR, immunofluorescence staining, colony formation assay, TUNEL and BrdU assay","Human, mice","H1299 cells, mouse embryonic fibroblasts, transgenic mice; colon, liver and kidney tumour samples; mouse xenograft","SLC7A11 :+: Cystine, Cystine :-: Ferroptosis","Validated","Cystine/glutamate transporter","Q9UPY5","XCT_HUMAN",25799988]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Ex vivo, Gpx4-deficient T cells rapidly accumulated membrane lipid peroxides and concomitantly underwent cell death driven by ferroptosis.","Viability assays, flow cytometry, lipid peroxidation assay","Mice","T cells","GPX4 :-: Ferroptosis","Validated","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",25824823]
["GCLC","Glutamate-cysteine ligase catalytic subunit","HGNC:4311","RNAi knockdown sensitized cell death induced by cystine starvation.","RNAi, qPCR","Mice","Mouse embryonic fibroblasts","GCLC :+: GSH, GSH :-: Ferroptosis","Validated","Glutamate--cysteine ligase catalytic subunit","P48506","GSH1_HUMAN",26166707]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Overexpression of SLC7A11 considerably abrogated ferroptosis.","Cell death quantification, gene transfection, western blot","Human","H1299 cells","SLC7A11 :-: Ferroptosis","Validated","Cystine/glutamate transporter","Q9UPY5","XCT_HUMAN",26218928]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","NRF2 plays a central role in protecting hepatocellular carcinoma (HCC) cells against ferroptosis","Cell Viability Analysis, western blot, RNAi, qRT-PCR","Human, mice","HepG2, Hepa1‐6, Hep3B, and SNU‐182 cells, C57BL/6 mice","NFE2L2 :+: (NQO1/HMOX1/FTH1), (NQO1/HMOX1/FTH1) :-: Ferroptosis","Validated","Nuclear factor erythroid 2-related factor 2","Q16236","NF2L2_HUMAN",26403645]
["SQSTM1","Sequestosome 1","HGNC:11280","The interaction between p62 and Keap1 increased following erastin and sorafenib treatment. Knockdown of p62 suppressed NRF2 expression and promoted growth inhibition with increased ferroptotic events including GSH depletion, lipid ROS production, and an increase of iron levels.","Immunoprecipitation assay, shRNA knockdown, iron assay, lipid peroxidation assay, glutathione assay, cell viability","Human, mice","HepG2, Hepa1‐6, Hep3B, and SNU‐182 cells","SQSTM1 :-: KEAP1, KEAP1 :-: NFE2L2, NFE2L2 :-: Ferroptosis","Validated","Sequestosome-1","Q13501","SQSTM_HUMAN",26403645]
["NQO1","NAD(P)H quinone dehydrogenase 1","HGNC:2874","Knockdown of p62, quinone oxidoreductase‐1, heme oxygenase‐1, and ferritin heavy chain‐1 by RNA interference in HCC cells promoted ferroptosis in response to erastin and sorafenib.","qRT‐PCR, RNA interference","Human, mice","HepG2, Hepa1‐6, Hep3B, and SNU‐182 cells","NQO1 :-: Ferroptosis","Validated","NAD(P)H dehydrogenase [quinone] 1","P15559","NQO1_HUMAN",26403645]
["HMOX1","Heme oxygenase 1","HGNC:5013","Knockdown of p62, quinone oxidoreductase‐1, heme oxygenase‐1, and ferritin heavy chain‐1 by RNA interference in HCC cells promoted ferroptosis in response to erastin and sorafenib.","qRT‐PCR, RNA interference","Human, mice","HepG2, Hepa1‐6, Hep3B, and SNU‐182 cells","HMOX1 :-: Ferroptosis","Validated","Heme oxygenase 1","P09601","HMOX1_HUMAN",26403645]
["FTH1","Ferritin heavy chain 1","HGNC:3976","Knockdown of p62, quinone oxidoreductase‐1, heme oxygenase‐1, and ferritin heavy chain‐1 by RNA interference in HCC cells promoted ferroptosis in response to erastin and sorafenib.","qRT‐PCR, RNA interference","Human, mice","HepG2, Hepa1‐6, Hep3B, and SNU‐182 cells","FTH1 :-: Ferroptosis","Validated","Ferritin heavy chain","P02794","FRIH_HUMAN",26403645]
["MUC1","Mucin 1, cell surface associated","HGNC:7508","MUC1-C (C-terminal subunit) blocks erastin-induced ferroptosis and induces increases in GSH.","shRNA, GSH level measurement, cell death test","Human","MDA-MB-468, BT-20 cells","MUC1 :+: System Xc-, System Xc- :+: GSH, GSH :-: Ferroptosis","Validated","Mucin-1","P15941","MUC1_HUMAN",26930718]
["SLC3A2","Solute carrier family 3 member 2","HGNC:11026","Required for in vitro cell survival because of its role in protecting cells from ferroptosis.","Cell survival test","Mice","Mouse ES-derived fibroblasts","SLC3A2 :+: System Xc-, System Xc- :-: Ferroptosis","Validated","4F2 cell-surface antigen heavy chain","P08195","4F2_HUMAN",26945935]
["MT1G","Metallothionein 1G","HGNC:7399","A negative regulator of ferroptosis in HCC cells. Knockdown of MT‐1G by RNA interference increases glutathione depletion and lipid peroxidation, which contributes to sorafenib‐induced ferroptosis.","RNA interference, cell viability assay, colony formation assay, western blot, qRT-PCR, glutathione assay, lipid peroxidation assay","Human, mice","HCC cells (HepaG2, Hep3B, and Huh7 cells), nude mice.","MT1G :-: Ferroptosis","Validated","Metallothionein-1G","P13640","MT1G_HUMAN",27015352]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Required for sorafenib‐induced expression of MT‐1G which is a ferroptosis suppressor.","shRNA, qRT-PCR, western blot","Human","Huh7 and/or HepG2 cells","NFE2L2 :+: MT1G, MT1G :-: Ferroptosis","Deduced","Nuclear factor erythroid 2-related factor 2","Q16236","NF2L2_HUMAN",27015352]
["SLC40A1","Solute carrier family 40 member 1","HGNC:10909","Involved in siramesine and lapatinib-induced ferroptotic cell death. Its expression is decreased after treatment with siramesine alone or in combination with lapatinib. Overexpression FPN resulted in decreased ROS and cell death whereas knockdown of FPN increased cell death after siramesine and lapatinib treatment.","Western blot, densitometry quantification, siRNA, gene transfection, cell death test","Human","MDA MB 231 and SKBr3 cells","SLC40A1 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Solute carrier family 40 member 1","Q9NP59","S40A1_HUMAN",27441659]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Knockdown increased cell death.","siRNA, western blot, cell death test","Human","MDA MB 231 cells","SLC7A11 :+: GSH, GSH :-: Ferroptosis","Validated","Cystine/glutamate transporter","Q9UPY5","XCT_HUMAN",27441659]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Knockdown increased cell death.","siRNA, western blot, cell death test","Human","MDA MB 231 and SKBr3 cells","GPX4 :-: Ferroptosis","Validated","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",27441659]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Inhibition induces ferroptosis. Silencing of the SLC7A11 gene increases the cisplatin sensitivity of resistant HNC cells.","Cell viability, western blot, siRNA or shRNA, glutathione and ROS production measurements","Human, mice","AMC-HN3R, -HN4R, and -HN9R head and neck cancer cells, BALB/c male nude mice","SLC7A11 :-: Ferroptosis","Validated","Cystine/glutamate transporter","Q9UPY5","XCT_HUMAN",27477897]
["CISD1","CDGSH iron sulfur domain 1","HGNC:30880","Genetic inhibition of CISD1 contributes to erastin-induced ferroptosis. Stabilization of the iron sulfur cluster of CISD1 inhibits ferroptosis.","Cytotoxicity assays, western blot, q-PCR, RNAi","Human","HepG2 and Hep3B cells","CISD1 :-: Mitochondrial lipid ROS, Mitochondrial lipid ROS :+: Ferroptosis","Validated","CDGSH iron-sulfur domain-containing protein 1","Q9NZ45","CISD1_HUMAN",27510639]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Elevated levels of expression are resistant to erastin-induced ferroptosis. Repression of SLC7A11 expression by p53 sensitized cells to undergo erastin-induced ferroptosis.","Western blot, cell death assay, qPCR","Human","H1299 cells","SLC7A11 :-: Ferroptosis","Validated","Cystine/glutamate transporter","Q9UPY5","XCT_HUMAN",27705786]
["FANCD2","FA complementation group D2","HGNC:3585","Inhibits erastin-induced ferroptosis. Plays a novel role in the negative regulation of ferroptosis.","Cell viability assay, Q-PCR, western blot, iron assay, lipid peroxidation assay, glutathione assay","Mice","Bone marrow stromal cells","FANCD2 :-: Ferroptosis","Validated","Fanconi anemia group D2 protein","Q9BXW9","FACD2_HUMAN",27773819]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Protects lipid peroxidation. Cell damage induced by GPx4 ablation is involved in ferroptosis.","RT-PCR, western blot, cytotoxicity assay, cell proliferation assay, siRNA","Human","Umbilical vein endothelial cell","GPX4 :-: LDH, LDH :+: Ferroptosis","Validated","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",27964880]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Nrf2 activation contributes to the resistance of HNCs to artesunate-induced ferroptosis. Nrf2 inhibition sensitizes head and neck cancer cells to artesunate-induced ferroptosis.","Cell viability assay, RNAi, gene transfection, western blot","Mice","Head and neck cancer cells (HN2–10), nude mice","NFE2L2 :-: Ferroptosis","Validated","Nuclear factor erythroid 2-related factor 2","Q16236","NF2L2_HUMAN",28012440]
["FTMT","Ferritin mitochondrial","HGNC:17345","Overexpression significantly inhibited erastin-induced ferroptosis.","Cell viability assay, western blot","Mice, fly","SH-SY5Y cells, drosophila melanogasters W1118, elav-Gal4 and UAS-Fer3HCH","FTMT :-: Labile iron pool, Labile iron pool :+: Ferroptosis","Validated","Ferritin","Q8N4E7","FTMT_HUMAN",28066232]
["HSPA5","Heat shock protein family A (Hsp70) member 5","HGNC:5238","Negatively regulates ferroptosis. Suppression of HSPA5 expression increased erastin-induced death. Overexpressed HSPA5 inhibited erastin-induced ferroptotic cell death.","Cell viability assay, western blot, shRNA, gene transfection","Human, mice","Pancreatic ductal adenocarcinoma cell lines (PANC1, CFPAC1, MiaPaCa2), nude mice, B6 mice","HSPA5 :+: GPX4, GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Endoplasmic reticulum chaperone BiP","P11021","BIP_HUMAN",28130223]
["ATF4","Activating transcription factor 4","HGNC:786","Inhibition of ATF4 expression increased erastin-induced cell death. ATF4 results in the induction of HSPA5, which in turn protects against GPX4 protein degradation and subsequent ferroptosis.","CCK8 cell viability assay, clonogenic cell survival assay, western blot, q-PCR, RNAi","Human","Pancreatic ductal adenocarcinoma cells (e.g., PANC1, CFPAC1, MiaPaCa2, and Panc2.03)","ATF4 :+: HSPA5, HSPA5 :+: GPX4, GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Cyclic AMP-dependent transcription factor ATF-4","P18848","ATF4_HUMAN",28130223]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Slc7a11 deletion increases susceptibility to iron overload-induced ferroptosis.","Cell viability, RNAi, RT-PCR","Mice","Mice, hepatocytes, bone marrow–derived macrophages","SLC7A11 :-: Ferroptosis","Validated","Cystine/glutamate transporter","Q9UPY5","XCT_HUMAN",28195347]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Ferroptosis drives neurodegeneration in Gpx4BIKO mice.","RT-qPCR, behavior test, western blot","Mice","Forebrain neurons of Gpx4BIKO mice and Gpx4(f/f) mice","GPX4 :-: Ferroptosis","Validated","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",28212525]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Downregulation conferred increased sensitivity to ferroptosis following cystine deprivation.","Immunoblotting, cell viability","Human","Human mammary epithelial cells","GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",28297659]
["HMOX1","Heme oxygenase 1","HGNC:5013","Demonsrates antiferroptotic role. HO-1 deficiency promotes erastin-induced ferroptosis.","RT-PCR, western blot, cell viability, siRNA","Mice","Renal proximal tubule cells","HMOX1 :-: Ferroptosis","Validated","Heme oxygenase 1","P09601","HMOX1_HUMAN",28515173]
["ATF4","Activating transcription factor 4","HGNC:786","ATF4 expression induces acquired cell death resistance. ATF4 knockdown renders cells susceptible for ferroptosis.","siRNA, cell transfection, cell viability assay, RT-PCR, immunoblotting","Human","U87 and U251 glioblastoma cells","ATF4 :+: xCT, xCT :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Cyclic AMP-dependent transcription factor ATF-4","P18848","ATF4_HUMAN",28553953]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Nrf2 over expression promotes resistance to ferroptosis.","Cell growth assay","Human, rat","F98, U87 glioma cells","NFE2L2 :+: xCT, xCT :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Nuclear factor erythroid 2-related factor 2","Q16236","NF2L2_HUMAN",28805788]
["TP53","Tumor protein p53","HGNC:11998","Inhibits ferroptosis in human colorectal cancer (CRC) cells. Loss of TP53 restored erastin sensitivity. Inhibits cell death induction by erastin in human CRC cells.","RNAi, live-cell imaging, cell viability, qPCR, western blot","Human, mice","HCT116, athymic nude mice","TP53 :+: Nucleus DPP4, Nucleus DPP4 :+: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","Cellular tumor antigen p53","P04637","P53_HUMAN",28813679]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Knockdown sensitized cells to erastin.","shRNA, cell viability","Human","HCT116","SLC7A11 :-: Ferroptosis","Validated","Cystine/glutamate transporter","Q9UPY5","XCT_HUMAN",28813679]
["HELLS","Helicase, lymphoid specific","HGNC:4861","LSH inhibits ferroptosis by decreasing the intracellular levels of iron and lipid ROS.","Plate-colony formation assay; measurement of total ROS, Lipid ROS, and intracellular iron","Human, mice","Lung cancer H358 and PC9 cells, SCID Mice","HELLS :+: GLUT1, HELLS :+: SCD, HELLS :+: FADS2, GLUT1 :-: Ferroptosis, SCD :-: Ferroptosis, FADS2 :-: Ferroptosis","Validated","Lymphoid-specific helicase","Q9NRZ9","HELLS_HUMAN",28900510]
["SCD","Stearoyl-CoA desaturase","HGNC:10571","Depletion of the SCD1 and FADS2 metabolic genes induces ferroptosis.","shRNA, RT-qPCR, MTT assay, total iron detection, lipid ROS detection","Human","A549 cancer cells","SCD :-: Ferroptosis","Validated","Acyl-CoA desaturase","O00767","ACOD_HUMAN",28900510]
["FADS2","Fatty acid desaturase 2","HGNC:3575","Depletion of the SCD1 and FADS2 metabolic genes induces ferroptosis.","shRNA, RT-qPCR, MTT assay, total iron detection, lipid ROS detection","Human","A549 cancer cells","FADS2 :-: Ferroptosis","Validated","Acyl-CoA 6-desaturase","O95864","FADS2_HUMAN",28900510]
["SRC","SRC proto-oncogene, non-receptor tyrosine kinase","HGNC:11283","Src-STAT3 activation renders the cell unable to undergo to ferroptosis. Src inhibition decreased cell viability significantly, and that loss of viability was rescued by ferroptosis inhibitors.","Immunoblotting, RNAi, qPCR, western blot, cell viability","Human","MCF-10A and SUM-159 cells","SRC :+: STAT3, STAT3 :-: ACSL4, ACSL4 :+: Ferroptosis","Validated","Proto-oncogene tyrosine-protein kinase Src","P12931","SRC_HUMAN",28972104]
["STAT3","Signal transducer and activator of transcription 3","HGNC:11364","Src-STAT3 activation renders the cell unable to undergo to ferroptosis.","Immunoblotting, RNAi, qPCR, western blot, cell viability","Human","MCF-10A and SUM-159 cells","STAT3 :-: ACSL4, ACSL4 :+: Ferroptosis","Validated","Signal transducer and activator of transcription 3","P40763","STAT3_HUMAN",28972104]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","ARF-mediated ferroptosis was largely abrogated by co-expression of NRF2.","Gene transfection, cell death quantification","Human, mice","H1299, SaoS2, U2OS cells, nude mice","NFE2L2 :+: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","Nuclear factor erythroid 2-related factor 2","Q16236","NF2L2_HUMAN",28985506]
["PML","Promyelocytic leukemia","HGNC:9113","PML expression turned cells highly resistant to ferroptosis.","Cell death assessment","Human","IMR90 cells","PML :-: Ferroptosis","Validated","Protein PML","P29590","PML_HUMAN",29081404]
["MTOR","Mechanistic target of rapamycin kinase","HGNC:3942","Necessary and sufficient to protect cardiomyocyte cells against ferroptotic cell death. mTOR overexpression suppressed ferroptotic cell death, whereas mTOR deletion exaggerated cell death.","Cell viability assay, ROS production assay, western blot","Mice","Cardiomyocyte cell, C57BL/6 mice","MTOR :-: Ferroptosis","Validated","Serine/threonine-protein kinase mTOR","P42345","MTOR_HUMAN",29127238]
["NFS1","NFS1 cysteine desulfurase","HGNC:15910","Suppression of NFS1 cooperates with inhibition of cysteine transport to trigger ferroptosis in vitro and slow tumour growth. Suppression of NFS1 predisposes cancer cells to ferroptosis.","RNAi, cell viability assay,flow cytometry","Human","MDA-MB-231 cells, NOD.CB17 Scid/J mice","NFS1 :-: TFRC, TFRC :+: Ferroptosis","Validated","Cysteine desulfurase","Q9Y697","NFS1_HUMAN",29168506]
["TP63","Tumor protein p63","HGNC:15979","Delta Np63 alpha can inhibit ferroptosis independent of p53. Overexpression protects cells from ferroptosis-inducing agents.","siRNA, cell death quantification","Human","ME-180 cells","TP63 :-: Ferroptosis","Validated","Tumor protein 63","Q9H3D4","P63_HUMAN",29212036]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Overexperession of SLC7A11 attenuated BAY-inhibited cell viability by ferroptosis.","Cell viability assay, qRT-PCR, shRNA, gene overexpression, western blot","Human","MDA-MB-231,  MDA-MB-468, DBTRG-05MG","SLC7A11 :-: Ferroptosis","Validated","Cystine/glutamate transporter","Q9UPY5","XCT_HUMAN",29274359]
["TP53","Tumor protein p53","HGNC:11998","p53 stabilization suppresses ferroptosis. p53 suppresses metabolic stress-induced ferroptosis.","Immunoblotting, cell death quantification, time-lapse imaging","Human","HT-1080 cells","TP53 :+: CDKN1A, CDKN1A :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Cellular tumor antigen p53","P04637","P53_HUMAN",29346757]
["CDKN1A","Cyclin dependent kinase inhibitor 1A","HGNC:1784","Required to to suppress ferroptosis.","CRISPR, shRNA, immunoblotting, cell-cycle profile, cell death quantification","Human","HT-1080 cells","CDKN1A :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Cyclin-dependent kinase inhibitor 1","P38936","CDN1A_HUMAN",29346757]
["MIR137","microRNA 137","HGNC:31523","Suppresses ferroptosis both in vitro and in vivo.","Immunoblotting, luciferase reporter assay, qRT-PCR, cell viability assay, colony formation assay, malondialdehyde assay, iron assay, flow cytometer, shRNA, xenograph mouse model","Human, mice","Melanoma cell lines A375 and G-361, C57BL/6 mice","MIR137 :-: SLC1A5, SLC1A5 :+: Glutaminolysis, Glutaminolysis :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","_NA_",null,29348676]
["SLC40A1","Solute carrier family 40 member 1","HGNC:10909","Overexpression of Fpn inhibited ferroptosis.","Cell viability assay, lipid ROS measurement, RT-qPCR, western blot","Mice","TM4 Sertoli cells","SLC40A1 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Solute carrier family 40 member 1","Q9NP59","S40A1_HUMAN",29436589]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Activation blocked ferroptosis.","Cell viability assay, lipid ROS measurement, RT-qPCR, western blot","Mice","TM4 Sertoli cells","GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",29436589]
["GPX4","Glutathione peroxidase 4","HGNC:4556","GPX4-overexpressing cells were resistant to reactive oxygen species-induced cell death. Conversely, GPX4-knockdown cells were sensitive to reactive oxygen species-induced cell death.","Immunohistochemistry, qRT-PCR,lentiviral transduction, shRNA, flow cytometry, cell viability assay","Human","LCL-K cell","GPX4 :-: 8-OHdG, 8-OHdG :+: Ferroptosis","Deduced","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",29463878]
["ENPP2","Ectonucleotide pyrophosphatase/phosphodiesterase 2","HGNC:3357","Overexpression modestly promotes migration and proliferation and significantly inhibits erastin-induced ferroptosis.","Cell viability assay, qRT-PCR, western blot, ROS measurement, ELISA","Rat","H9c2 cells","ENPP2 :-: Ferroptosis","Validated","Ectonucleotide pyrophosphatase/phosphodiesterase family member 2","Q13822","ENPP2_HUMAN",29551679]
["VDAC2","Voltage dependent anion channel 2","HGNC:12672","Overexpression could partially protect cells from ferroptosis.","Western blot, cell viability assay","Human","HT1080 cells","VDAC2 :-: Ferroptosis","Validated","Voltage-dependent anion-selective channel protein 2","P45880","VDAC2_HUMAN",29569437]
["FH","Fumarate hydratase","HGNC:3700","FH inactivation (FH-/- ) proves synthetic lethal with inducers of ferroptosis. FH-/- sensitizes cells to multiple ferroptosis inducers.","CRISPR/Cas9, sgRNA, cell viability assay, immunoblotting, qPCR, ","Human","UOK262, HT1080, HK2, A498 cells","FH :+: GPX4, GPX4 :-: Ferroptosis","Validated","Fumarate hydratase","P07954","FUMH_HUMAN",29917289]
["CISD2","CDGSH iron sulfur domain 2","HGNC:24212","Overexpression conferred resistance to ferroptosis. Inhibition blocked resistance to ferroptotic cell death.","Cell viability and death assay, GSH and ROS levels and lipid peroxidation assays, RNAi, gene transfection, immunoblotting","Human","Head and neck cancer cells","CISD2 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","CDGSH iron-sulfur domain-containing protein 2","Q8N5K1","CISD2_HUMAN",29928961]
["SLC40A1","Solute carrier family 40 member 1","HGNC:10909","A negative regulator of ferroptosis by reducing intracellular iron concentration. Knockdown accelerates erastin-induced ferroptosis.","Cell viability, qRT-PCR, western blot, siRNA","Human","SH-SY5Y cells","SLC40A1 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Solute carrier family 40 member 1","Q9NP59","S40A1_HUMAN",29949159]
["MIR9-1","microRNA 9-1","HGNC:31641","Overexpression of miR‐9 suppressed GOT1, which subsequently reduced ferroptosis. Suppression of miR‐9 increased the sensitivity of melanoma cells to ferroptosis inducers.","Immunoblotting, luciferase reporter assay, qPCR, cell viability assay, MDA assay, iron assay, glutamate assay","Human","A375 and G‐361 cells","MIR9 :-: GOT1, GOT1 :+: alpha KG, alpha KG :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","_NA_",null,30035324]
["MIR9-2","microRNA 9-2","HGNC:31642","Overexpression of miR‐9 suppressed GOT1, which subsequently reduced ferroptosis. Suppression of miR‐9 increased the sensitivity of melanoma cells to ferroptosis inducers.","Immunoblotting, luciferase reporter assay, qPCR, cell viability assay, MDA assay, iron assay, glutamate assay","Human","A375 and G‐361 cells","MIR9 :-: GOT1, GOT1 :+: alpha KG, alpha KG :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","_NA_",null,30035324]
["MIR9-3","microRNA 9-3","HGNC:31646","Overexpression of miR‐9 suppressed GOT1, which subsequently reduced ferroptosis. Suppression of miR‐9 increased the sensitivity of melanoma cells to ferroptosis inducers.","Immunoblotting, luciferase reporter assay, qPCR, cell viability assay, MDA assay, iron assay, glutamate assay","Human","A375 and G‐361 cells","MIR9 :-: GOT1, GOT1 :+: alpha KG, alpha KG :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","_NA_",null,30035324]
["CBS","Cystathionine beta-synthase","HGNC:1550","Inhibition triggers ferroptosis in hepatocellular carcinoma.","Cell viability, western blotting, LC/ESI-MS/MS, flow cytometry, xenograft mice","Human, mice","HepG2 cells, female ICR mice","CBS :-: Ferroptosis","Validated","Cystathionine beta-synthase","P35520","CBS_HUMAN",30258181]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Associated with resistance to ferroptosis. Inhibition of Nrf2 sensitized cells to ferroptosis.","Cell viability and cell death assays, ROS production measurement, RNAi, gene transfection, immunoblotting","Human, mice","HNC cell line (AMC-HN2–11), SNUcell lines (SNU-1041, -1066, and -1076), athymic BALB/c male nude mice","NFE2L2 :+: FTH1, FTH1 :-: Ferroptosis, NFE2L2 :+: FPN, FPN :-: Ferroptosis, NFE2L2 :+: HO-1, HO-1 :-: Ferroptosis","Validated","Nuclear factor erythroid 2-related factor 2","Q16236","NF2L2_HUMAN",30339884]
["SQSTM1","Sequestosome 1","HGNC:11280","Inhibition of the p62 gene significantly reduced cell viability and increased cellular lipid ROS levels in HN3R cells; this was reversed by treatment with ferrostatin-1, a ferroptosis inducer.","siRNA, qPRC, fluorescent microscopy and flow cytometry, ROS production measurement","Human","HN3 cells","SQSTM1 :+: NFE2L2, NFE2L2 :+: FTH1, FTH1 :-: Ferroptosis, NFE2L2 :+: FPN, FPN :-: Ferroptosis, NFE2L2 :+: HO-1, HO-1 :-: Ferroptosis","Validated","Sequestosome-1","Q13501","SQSTM_HUMAN",30339884]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Overexpression GPX4 resulted in decreased cell death after RSL3 treatment. Moreover, this effect was able to be reversed by overexpression of GPX4.","Cell viability, cell death, ROS determination, western blot","Human","HCT116, LoVo, and HT29 CRC cells","GPX4 :-: Ferroptosis","Validated","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",30524291]
["ISCU","Iron-sulfur cluster assembly enzyme","HGNC:29882","Over expression significantly attenuated DHA induced ferroptosis.","Cell viability, cell cycle, ROS determination, western blot, immunofluorescence, ectopic expressing, lentiviral transduction","Human","HL60, KG1, THP-1 cells","ISCU :+: GSH, GSH :-: Ferroptosis","Validated","Iron-sulfur cluster assembly enzyme ISCU","Q9H1K1","ISCU_HUMAN",30557609]
["FTH1","Ferritin heavy chain 1","HGNC:3976","FTH reconstituted cells exhibited the reduced lipid peroxides content and restored the DHA-induced ferroptosis.","Cell viability, cell cycle, ROS determination, western blot, immunofluorescence, ectopic expressing, lentiviral transduction","Human","HL60, KG1, THP-1 cells","FTH1 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Ferritin heavy chain","P02794","FRIH_HUMAN",30557609]
["ACSL3","Acyl-CoA synthetase long chain family member 3","HGNC:3570","Required for exogenous monounsaturated fatty acids to protect cells against ferroptosis. Negatively correlates with ferroptosis sensitivity.","Dead cell count, gene knockout, confocal imaging, thin-layer chromatography, BODIPY 493/503 imaging, CRISPR/Cas9, RT-qPCR, western blot","Human","HEK293, HT-1080 cells","ACSL3 :-: Ferroptosis","Validated","Long-chain-fatty-acid--CoA ligase 3","O95573","ACSL3_HUMAN",30686757]
["OTUB1","OTU deubiquitinase, ubiquitin aldehyde binding 1","HGNC:23077","Inactivation promotes ferroptosis by down-regulating SLC7A11 levels. Overexpression is critical for tumor growth.","CRISPR/Cas9, RNAi, cell death, western blot, qRT-PCR","Human, mice","H1299, SK-N-BE(2)C, T24, UM-UC-3, SW780 cells, nude mice","OTUB1 :+: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","Ubiquitin thioesterase OTUB1","Q96FW1","OTUB1_HUMAN",30709928]
["CD44","CD44 molecule (Indian blood group)","HGNC:1681","Knockdown sensitizes cells to ferroptosis.","Western blot, siRNA, cell death","Human","H1299 cells","CD44 :+: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","CD44 antigen","P16070","CD44_HUMAN",30709928]
["LINC00336","Long intergenic non-protein coding RNA 336","HGNC:33813","Overexpression inhibits ferroptosis. Knockdown promotes ferroptosis.","qRT-PCR, MTT assay, colony-formation assay, lipid ROS assays, iron assay, immunoblotting","Human","A549 and SPC-A-1 cells","(LINC00336/ELAVL1) :-: Ferroptosis","Validated","Putative uncharacterized protein encoded by LINC00336","Q6ZUF6","NC336_HUMAN",30787392]
["STAT3","Signal transducer and activator of transcription 3","HGNC:11364","Upregulated in ferroptosis resistant cells. Inhibition increases ferroptosis.","Cell viability tests, ROS and lipid peroxidation measures, MDA measurement, western blot","Human","Osteosarcoma cells MG63 and Saos-2","STAT3 :-: Ferroptosis","Validated","Signal transducer and activator of transcription 3","P40763","STAT3_HUMAN",30811078]
["BRD4","Bromodomain containing 4","HGNC:13575","Inhibition induces ferroptosis.","Cell proliferation assay, migration and invasion assays, RT-PCR, western blot, immunohistochemistry, iron assay","Human","MDA-MB-231, Hs578T, H1299, A549 and MCF-10A cells, female athymic BALB/c nude mice","BRD4 :-: Ferritinophagy, Ferritinophagy :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Bromodomain-containing protein 4","O60885","BRD4_HUMAN",30988278]
["PRDX6","Peroxiredoxin 6","HGNC:16753","A negative regulator of ferroptotic cell death.","Western blot, flow cytometry, SRB assay, gene transfection, shRNA, LOOH quantification","Human","H1299 cells","PRDX6 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Peroxiredoxin-6","P30041","PRDX6_HUMAN",31036877]
["MIR17","microRNA 17","HGNC:31547","Protects endothelial HUVEC cells from erastin-induced ferroptosis. Overexpression significantly reduced erastin-induced growth inhibition and ROS generation of HUVEC cells.","Lentivirus transduction, CCK-8 assay, DYE670 staining assay, ROS staining assay, RT-qPCR, western blot","Human","HUVEC cells","MIR17 :-: A20, A20 :+: ACSL4, ACSL4 :+: Lipid ROS, A20 :-: GPX4, GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","_NA_",null,31160087]
["SCD","Stearoyl-CoA desaturase","HGNC:10571","Inhibition of SCD1 induces ferroptotic cell death. Expression of SCD1 protects cells from ferroptosis.","Cell viability, cell death, BODIPY staining, siRNA, RT-qPCR, western blot, gene overexpression, ","Human, mice","OVCAR-4, COV362, FT-t cells, mouse orthotopic xenograft model","SCD :+: CoQ10, CoQ10 :-: Ferroptosis","Validated","Acyl-CoA desaturase","O00767","ACOD_HUMAN",31270077]
["SESN2","Sestrin 2","HGNC:20746","Has cytoprotective effect against ferroptosis. In cells expressing Sesn2, erastin-induced cell death, ROS formation, and glutathione depletion were almost completely inhibited compared to that in control cells.","MTT assay, RT-PCR, luciferase assay, immunoblot, siRNA, Lipid peroxidation assay, GSH measurement","Human, mice","HepG2 and AML-12 cell lines, mouse embryonic fibroblasts cells, ICR mice","SESN2 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Sestrin-2","P58004","SESN2_HUMAN",31323261]
["NF2","Neurofibromin 2","HGNC:7773","Genetic inactivation of NF2 rendered cancer cells more sensitive to ferroptosis. Mediates cell density-dependent inhibition of ferroptosis.","Cell death, cell viability, lipid peroxidation, immunoblotting, CRISPR/Cas9, ChIP assay, qRT-PCR","Human, mice","Mouse embryonic fibroblasts, NF639 cells, human epithelial tumour cells, human mesothelioma cells, xenograft mouse","NF2 :-: YAP, YAP :+: Ferroptosis","Validated","Merlin","P35240","MERL_HUMAN",31341276]
["ARNTL","Aryl hydrocarbon receptor nuclear translocator like","HGNC:701","Degration of the protein is critical for ferroptosis. Blocking ARNTL degradation diminished ferroptotic tumor cell death.","Cytotoxicity assays, western blot, immunoprecipitation, RNA interference, gene transfection, qPCR, MDA assay","Human, mice","Calu-1, THP1, HT1080, and HL-60 cells, athymic nude mice","ARNTL :-: EGLN2, EGLN2 :-: HIF1A, HIF1A  :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Aryl hydrocarbon receptor nuclear translocator-like protein 1","O00327","BMAL1_HUMAN",31355331]
["HIF1A","Hypoxia inducible factor 1 subunit alpha","HGNC:4910","Destabilizing HIF1A facilitated ferroptotic tumor cell death.","Cytotoxicity assays, western blot, immunoprecipitation, RNA interference, gene transfection, qPCR, MDA assay","Human, mice","Calu-1, THP1, HT1080, and HL-60 cells, athymic nude mice","HIF1A  :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Hypoxia-inducible factor 1-alpha","Q16665","HIF1A_HUMAN",31355331]
["JUN","Jun proto-oncogene, AP-1 transcription factor subunit","HGNC:6204","O-GlcNAcylated c-Jun represents an obstructive factor to ferroptosis.","Immunofluorescence, western blotting, immunohistochemistry, cell viability, colony formation, qPCR, lipid peroxidation assay, iron assay","Human, mice","Bel-7402, SMMC-7721 cells, athymic nude mice","JUN :+: GSH, GSH :-: Ferroptosis, JUN :+: PSAT1, PSAT1 :-: Ferroptosis, JUN :+: CBS, CBS :-: Ferroptosis","Deduced","Transcription factor AP-1","P05412","JUN_HUMAN",31394193]
["CA9","Carbonic anhydrase 9","HGNC:1383","Inhibition induces ferroptosis.","qRT-PCR, western blot, cell viability assay, siRNA, ROS detection","Human","Mesothelioma cell lines ACC-Meso-1, NCI-H2373 and NCI-H2052, mesothelial cell line MeT-5A","CA9 :-: Ferroptosis","Validated","Carbonic anhydrase 9","Q16790","CAH9_HUMAN",31442913]
["HSPA5","Heat shock protein family A (Hsp70) member 5","HGNC:5238","Knockdown of GRP78 enhanced artesunate-induced ferroptosis of pancreatic cancer cells.","Fluorescent staining, CCK8, Western blot, shRNA, qRT-PCR","Human, mice","Pancreatic cancer cells AsPC-1 and PaTU8988, BALB/c nude mice","GRP78 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Endoplasmic reticulum chaperone BiP","P11021","BIP_HUMAN",31456633]
["TMBIM4","Transmembrane BAX inhibitor motif containing 4","HGNC:24257","Protects against ferroptosis in HCC cells. Inhibition increased ferroptotic cel death.","Cell viability and clonogenic survival assay, immunofluorescence, western blot, RNAi, gene transfection, qRT-PCR, lipid peroxidation assay","Human, mice","Hep G2, Huh‐7, SMMC‐7721 and PLC/PRF/5 cells, nude mice","S1R :-: Ferroptosis","Validated","Protein lifeguard 4","Q9HC24","LFG4_HUMAN",31507082]
["HSPA5","Heat shock protein family A (Hsp70) member 5","HGNC:5238","Serves as a negative regulator of DHA-induced ferroptosis.","Cell viability, colony formation, cell death, lipid ROS assay, GSH determination, siRNA, western blot, RT-PCR, xenograft tumor model","Human, mice","Glioma cell lines U251 and U373, mouse hippocampal neuronal cell line HT22, athymic nude BALB/c mice","HSPA5 :+: GPX4, GPX4 :-: Ferroptosis","Predicted","Endoplasmic reticulum chaperone BiP","P11021","BIP_HUMAN",31519193]
["PLIN2","Perilipin 2","HGNC:248","An indispensable gene and protein in the suppression of ferroptosis caused by abnormal lipometabolism in gastric carcinoma.","Cell proliferation and clonogenic assay, cell survival analysis, xenografts tumor, RNA-seq, RT?qPCR, ROS assay","Human, mice","SGC7901 and MGC803 cells, nude mice","PLIN2 :-: Ferroptosis","Screened","Perilipin-2","Q99541","PLIN2_HUMAN",31520166]
["MIR212","microRNA 212","HGNC:31589","Overexpression of miR-212-5p attenuated ferroptosis while downregulation of miR-212-5p promoted ferroptotic cell death.","Controlled cortical impact model, iron assay, MDA assay, RT-PCR, western blotting, cell death","Mice","HT22 and Neuro-2a cells, C57BL/6 J mice","(miR-212-5p/Ptgs2) :-: Ferroptosis","Validated","_NA_","_NA_",null,31533781]
["Fer1HCH","Ferritin 1 Heavy Chain Homolog","_NA_","Reduced heavy chain levels caused severe mitochondrial defects and ferroptosis.","RT-qPCR, immunohistochemistry, EdU staining, TUNEL staining","Drosophila","Fly","Fer1HCH :-: ROS, ROS :+: Ferroptosis","Predicted","Ferritin","H1UUD2","H1UUD2_DROME",31568497]
["AIFM2","Apoptosis inducing factor mitochondria associated 2","HGNC:21411","A glutathione-independent ferroptosis suppressor. Pharmacological targeting of FSP1 strongly synergizes with GPX4 inhibitors to trigger ferroptosis.","Expression cloning, cell death, cell viability, LDH assay, western blot, LC-MS, NADH consumption assay","Human, mice","MCF-7, HT1080, Pfa1 cells","FSP1 :+: CoQ10, CoQ10 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Apoptosis-inducing factor 2","Q9BRQ8","AIFM2_HUMAN",31634899]
["AIFM2","Apoptosis inducing factor mitochondria associated 2","HGNC:21411","A potent ferroptosis-resistance factor. Positively correlates with ferroptosis resistance.","Synthetic lethal CRISPR-Cas9 screen, cell death, western blotting, lipid droplet fractionation, glutathione measurement, tumour xenograft growth, CoQ measurement","Human, mice","U-2 OS cell line, NCI-H460, NCI-H2291, NCI-H1703 and NCIH446 cells, C.B17 SCID mice","FSP1 :+: CoQ10, CoQ10 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Apoptosis-inducing factor 2","Q9BRQ8","AIFM2_HUMAN",31634900]
["LAMP2","Lysosomal associated membrane protein 2","HGNC:6501","Knockdown promoted ferroptosis.","Cell viability, western blot, ROS induction, glutathione assay, lipid peroxidation assay","Human","ARPE-19, hfRPE cells","LAMP2 :+: Cysteine, Cysteine :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Lysosome-associated membrane glycoprotein 2","P13473","LAMP2_HUMAN",31672277]
["ZFP36","ZFP36 ring finger protein","HGNC:12862","ZFP36 plasmid impaired FBXW7 plasmid-induced HSC ferroptosis. Overexpression of Zfp36 impaired erastin- or sorafenib-induced ferroptosis.","Cell viability, lipid peroxidation, iron, GSH and ROS assays, western blot, RT-PCR, RNA-seq","Human, mice","Hepatic stellate cells, C57BL/6 mice","ZFP36 :-: ATG16L1, ATG16L1 :+: Ferroptosis","Validated","mRNA decay activator protein ZFP36","P26651","TTP_HUMAN",31679460]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Depletion or inhibition resulted in cell death by ferroptosis.","Quantitative proteomic analysis, siRNA, RNA-seq and qRT-PCR, cell viability, lipid peroxidation assay, GSH measurement, western blot","Human, mice, rat","H9c2 and C2C12 cells, rat ventricular myocytes, C57BL/6 mice","GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",31685805]
["PROM2","Prominin 2","HGNC:20685","Induced by ferroptotic stress and promotes resistance to ferroptotic cell death. Facilitates ferroptosis resistance in mammary epithelial and breast carcinoma cells.","RNA-seq, western blot, qPCR, siRNA, cell count, cell death, iron assay","Human","MCF10A, Hs578t and MDA-MB-231 cells","Prominin2 :+: Ferritin-containing MVB, Ferritin-containing MVB :-: Ferroptosis","Validated","Prominin-2","Q8N271","PROM2_HUMAN",31735663]
["CHMP5","Charged multivesicular body protein 5","HGNC:26942","Ferroptosis activators increase ESCRT-III subunits (e.g., CHMP5 and CHMP6). Knockdown of CHMP5 or CHMP6 sensitizes human cancer cells to ferroptosis.","RNAi, western blot, iron, malondialdehyde and cytotoxicity assays","Human, mice","PANC1 and HepG2 cells, athymic nude or B6 mice","CHMP5 :-: Ferroptosis","Validated","Charged multivesicular body protein 5","Q9NZZ3","CHMP5_HUMAN",31761326]
["CHMP6","Charged multivesicular body protein 6","HGNC:25675","Ferroptosis activators increase ESCRT-III subunits (e.g., CHMP5 and CHMP6). Knockdown of CHMP5 or CHMP6 sensitizes human cancer cells to ferroptosis.","RNAi, western blot, iron, malondialdehyde and cytotoxicity assays","Human, mice","PANC1 and HepG2 cells, athymic nude or B6 mice","CHMP6 :-: Ferroptosis","Validated","Charged multivesicular body protein 6","Q96FZ7","CHMP6_HUMAN",31761326]
["AKR1C1","Aldo-keto reductase family 1 member C1","HGNC:384","Inhibition completely resensitizes resistant melanoma cells to ferroptosis execution.","RNAi, cell transfection, western blotting, qRT-PCR, cell viability, lipid peroxides evaluation","Human","MeWo, A2058, SK-Mel 5, SK-Mel 24, C8161, CHL-1, and A375 cells","AKR1C1 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Aldo-keto reductase family 1 member C1","Q04828","AK1C1_HUMAN",31780644]
["AKR1C2","Aldo-keto reductase family 1 member C2","HGNC:385","Inhibition completely resensitizes resistant melanoma cells to ferroptosis execution.","RNAi, cell transfection, western blotting, qRT-PCR, cell viability, lipid peroxides evaluation","Human","MeWo, A2058, SK-Mel 5, SK-Mel 24, C8161, CHL-1, and A375 cells","AKR1C2 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Aldo-keto reductase family 1 member C2","P52895","AK1C2_HUMAN",31780644]
["AKR1C3","Aldo-keto reductase family 1 member C3","HGNC:386","Inhibition completely resensitizes resistant melanoma cells to ferroptosis execution.","RNAi, cell transfection, western blotting, qRT-PCR, cell viability, lipid peroxides evaluation","Human","MeWo, A2058, SK-Mel 5, SK-Mel 24, C8161, CHL-1, and A375 cells","AKR1C3 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Aldo-keto reductase family 1 member C3","P42330","AK1C3_HUMAN",31780644]
["CBS","Cystathionine beta-synthase","HGNC:1550","Knockdown in erastin-resistant cells caused ferroptotic cell death, while overexpression conferred ferroptosis resistance.","RNAi, cell viability, ROS analysis, GSH, glutamate release and cystine uptake assays, western blotting, qRT-PCR","Human","SKOV3 and OVCA429 cells","CBS :+: Cystathionine, Cystathionine :+: Cysteine, Cysteine :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Cystathionine beta-synthase","P35520","CBS_HUMAN",31819185]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Genetically repression of NRF2 enhanced ferroptosis susceptibility.","RNAi, cell viability, ROS analysis, GSH, glutamate release and cystine uptake assays, western blotting, qRT-PCR","Human","SKOV3 and OVCA429 cells","NFE2L2 :+: CBS, CBS :+: Cystathionine, Cystathionine :+: Cysteine, Cysteine :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Nuclear factor erythroid 2-related factor 2","Q16236","NF2L2_HUMAN",31819185]
["CAV1","Caveolin 1","HGNC:1527","Cav-1 deficiency aggravated ferroptosis. Short hairpin RNA of Cav-1 promoted ferroptosis, which was ameliorated by Cav-1 overexpression. ","Immunofluorescence, western blot, ROS detection","Human, mice","LO2 cells, mice model","CAV-1 :+: xCT, xCT :+: GPX4, GPX4 :-: Ferroptosis, CAV-1 :-: RNS, RNS :+: Ferroptosis","Validated","Caveolin-1","Q03135","CAV1_HUMAN",31877357]
["GCH1","GTP cyclohydrolase 1","HGNC:4193","Gch1 overexpression and its downstream metabolites BH4/BH2 rescue from ferroptosis. Inhibition of GCH1 activity can sensitize resistant cancer cells to ferroptosis induction.","CRISPR activation screening, qRT-PCR, cell viability, cell counts, western blot","Human","HT-1080,786-O, A-498, Caki-1, AU565, DU4475, MCF-7 cells","GCH1 :+: Phospholipid, Phospholipid :-: Ferroptosis, GCH1 :+: CoQ10, CoQ10 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","GTP cyclohydrolase 1","P30793","GCH1_HUMAN",31989025]
["SIRT3","sirtuin 3","HGNC:14931","These data indicate that inactivation of Sirt3 significantly sensitizes cells to p53-dependent ferroptosis upon ROS-induced stress.","CRISPR/Cas9, western blot","Human","U2OS cells","SIRT3 :-: Ferroptosis","Validated","NAD-dependent protein deacetylase sirtuin-3, mitochondrial","Q9NTG7","SIR3_HUMAN",33377976]
["DAZAP1","DAZ associated protein 1","HGNC:2683","Furthermore, DAZAP1 significantly reduced cellular sensitivity to sorafenib (SF), which had been proven to be an inducer of ferroptosis by targeting the system Xc?.","_NA_","_NA_","_NA_","(DAZAP1/SLC7A11) :-: Ferroptosis","Validated","DAZ-associated protein 1","Q96EP5","DAZP1_HUMAN",33358859]
["PIR","pirin","HGNC:30048","Consequently, in cell cultures and xenograft mouse models, blocking PIR signaling enhances ferroptosis-mediated tumor growth suppression.","_NA_","Human, mice","pancreatic cancer cells, xenograft mouse models","PIR :-: oxidative DNA damage, oxidative DNA damage :+: HMGB1, HMGB1 :+: ACSL4, ACSL4 :+: Ferroptosis","Validated","Pirin","O00625","PIR_HUMAN",33373853]
["GCLC","glutamate-cysteine ligase catalytic subunit","HGNC:4311","Non-canonical Glutamate-Cysteine Ligase Activity Protects against Ferroptosis","_NA_","Human","non-small-cell lung cancer cell lines","GCLC :+: gamma-glutamyl-peptides, gamma-glutamyl-peptides :-: Glu, Glu :+: Ferroptosis","Validated","_NA_","_NA_",null,33357455]
["FTL","ferritin light chain","HGNC:3999","Ferritin upregulation prevents ferroptosis by mediating P53 and SLC7A11 expression.","_NA_","Rat","_NA_","FTL :-: p53, FTL :-: SLC7A11, p53 :+: Ferroptosis, SLC7A11 :+: Ferroptosis","Validated","_NA_","_NA_",null,33333054]
["HCAR1","hydroxycarboxylic acid receptor 1","HGNC:4532","These observations support our hypothesis that HCAR1 and MCT1 are involved in the lactate-dependent ferroptosis resistance in tumor cells. Blocking the HCAR1/MCT1-Mediated Lactate Import Exacerbates Ferroptosis","Q-PCR, Immunohistochemistry analysis, siRNA, Western blot, Flow cytometeric analysis, Lipidomic study, animal study","Human, mice","HCC cells, mice model","(HCAR1/MCT1) :-: (AMP/ATP), (AMP/ATP) :+: AMPK, AMPK :-: SREBP1, SREBP1 :+: SCD1, SCD1 :-: Ferroptosis, (HCAR1/MCT1) :-: ACSL4, ACSL4 :+: Ferroptosis","Validated","_NA_","_NA_",null,33296645]
["SLC16A1","solute carrier family 16 member 1","HGNC:10922","These observations support our hypothesis that HCAR1 and MCT1 are involved in the lactate-dependent ferroptosis resistance in tumor cells. Blocking the HCAR1/MCT1-Mediated Lactate Import Exacerbates Ferroptosis","Q-PCR, Immunohistochemistry analysis, siRNA, Western blot, Flow cytometeric analysis, Lipidomic study, animal study","Human, mice","HCC cells, mice model","(HCAR1/MCT1) :-: (AMP/ATP), (AMP/ATP) :+: AMPK, AMPK :-: SREBP1, SREBP1 :+: SCD1, SCD1 :-: Ferroptosis, (HCAR1/MCT1) :-: ACSL4, ACSL4 :+: Ferroptosis","Validated","_NA_","_NA_",null,33296645]
["RRM2","ribonucleotide reductase regulatory subunit M2","HGNC:10452","RRM2 exerts an anti-ferroptotic role in liver cancer cells by sustaining GSH synthesis.","ELISA, qPCR, immunoblotting, Cell viability and cell death, Metabolites, immunofluorescence, co-immunoprecipitation, proximal ligation assay","Human","HepG2 and SMMC-7721 cells","RRM2 :+: GSS, GSS :+: GSH, GSH :-: Ferroptosis","Validated","_NA_","_NA_",null,33372599]
["SCD","stearoyl-CoA desaturase","HGNC:10571","Genetic and Pharmacological Inhibition of SCD1 Prevents the Growth of STK11/KEAP1 Co-mutant Cells and Sensitizes Those Cells to Ferroptosis Induction.","CRISPR, RNA-seq, cell gowth and viability assays","Human","H358-NTC, H358, A549 cells","SCD :+: Ferroptosis","Validated","_NA_","_NA_",null,33264619]
["NR4A1","nuclear receptor subfamily 4 group A member 1","HGNC:7980","Pharmacological inhibition of NR4A1 promotes ferroptosis in pancreatic cancer cells","Cell viability and cell death, siRNA, Western blot, qPCR, ChIP, MDA and GSH/GSSG assay","Human","cell lines PANC-1 and SW1990","NR4A1 :+: SCD1, SCD1 :-: Ferroptosis","Deduced","_NA_","_NA_",null,33271455]
["PIK3CA","phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha","HGNC:8975","Notably, we found that cancer cells carrying PIK3CA activating mutation or PTEN deletion appeared to be more resistant to RSL3. These mutations lead to the activation of the oncogenic PI3K-AKT signaling pathway. Therefore, sustained activation of the PI3K-AKT-mTOR pathway protects cancer cells from ferroptotic cell death.","Cell death","Human","BT474, MDA-MB-453","PIK3CA :+: PI3K-AKT pathway, PI3K-AKT pathway :-: Ferroptosis","Deduced","_NA_","_NA_",null,33229547]
["RPTOR","regulatory associated protein of MTOR complex 1","HGNC:30287","mTORC1, Instead of mTORC2, Suppresses Ferroptosis. short-hairpin RNA (shRNA)-mediated silencing of RAPTOR (a component of mTORC1) but not that of RICTOR (a component of mTORC2) sensitized MDA-MB-453 and BT474 cells to RSL3.","Cell death, Western blot, shRNA, ectopic expression","Human","BT474, MDA-MB-453","RAPTOR :+: mTORC1, mTORC1 :+: SREBP1, SREBP1 :+: SCD1, SCD1 :-: Ferroptosis","Deduced","_NA_","_NA_",null,33229547]
["SREBF1","sterol regulatory element binding transcription factor 1","HGNC:11289","SREBP1 Protects Cells from Ferroptosis through SCD1 Activity.","CRISPR/Cas9, overexpression, RT-PCR, Cell viability","Human","BT474, MDA-MB-453","SREBP1 :+: SCD1, SCD1 :-: Ferroptosis","Validated","_NA_","_NA_",null,33229547]
["SREBF2","sterol regulatory element binding transcription factor 2","HGNC:11290","The lipogenesis regulator SREBP2 directly induces transcription of the iron carrier Transferrin (TF), reducing intracellular iron pools, reactive oxygen species, and lipid peroxidation, thereby conferring resistance to inducers of ferroptosis.","_NA_","Human","_NA_","SREBP2 :+: TF, TF :-: ROS, ROS :+: Lipid peroxidation, Lipid peroxidation :+: Ferroptosis","Validated","_NA_","_NA_",null,33203734]
["FZD7","frizzled class receptor 7","HGNC:4045","Conversely, FZD7 overexpression induced increased GPX4 mRNA and protein expression levels. FZD7 Marks a Cell Population Susceptible to GPX4 Inhibitors.","_NA_","Human, mice","_NA_","FZD7 :+: GPX4, GPX4 :-: Ferroptosis","Validated","_NA_","_NA_",null,33172933]
["NFE2L2","nuclear factor, erythroid 2 like 2","HGNC:7782","In FRDA fibroblasts, both treatments promoted the nuclear translocation of Nrf2 (Fig. 5A) and the inhibition of redox imbalance and ferroptosis.","_NA_","Mice","_NA_","Nrf2 :-: ROS, ROS :+: Ferroptosis","Validated","_NA_","_NA_",null,33197769]
["NFE2L2","nuclear factor, erythroid 2 like 2","HGNC:7782","Up-regulating Nrf2 alleviated the diabetes related ferroptosis in kidneys.","_NA_","Mice","_NA_","Nrf2 :-: Ferroptosis","Validated","_NA_","_NA_",null,33152439]
["P4HB","prolyl 4-hydroxylase subunit beta","HGNC:8548","Knockdown of these target genes sensitized cells to known ferroptosis inducers, while PACMA31, a previously reported inhibitor of P4HB, directly induced ferroptosis and was highly synergistic with erastin.","_NA_","Human","_NA_","P4HB :-: ER stress, ER stress :+: Ferroptosis","Deduced","_NA_","_NA_",null,33124817]
["NT5DC2","5'-nucleotidase domain containing 2","HGNC:25717","Knockdown of these target genes sensitized cells to known ferroptosis inducers, while PACMA31, a previously reported inhibitor of P4HB, directly induced ferroptosis and was highly synergistic with erastin.","_NA_","_NA_","_NA_","NT5DC2 :-: Ferroptosis","Deduced","_NA_","_NA_",null,33124817]
["BCAT2","branched chain amino acid transaminase 2","HGNC:977","Here, by using a high-throughput CRISPR/Cas9-based genetic screen in HepG2 hepatocellular carcinoma cells to search for metabolic proteins inhibiting ferroptosis, we identified a branched-chain amino acid aminotransferase 2 (BCAT2) as a novel suppressor of ferroptosis.","_NA_","Human","_NA_","BCAT2 :-: Ferroptosis","Validated","_NA_","_NA_",null,33097833]
["HSF1","Heat shock transcription factor 1","HGNC:5224","Altogether, HSF1 may function as a key defender against PA-induced ferroptosis in cardiomyocytes by maintaining cellular iron homeostasis and GPX4 expression.","_NA_","Human, mice","_NA_","HSF1 :-: Ferroptosis","Validated","_NA_","_NA_",null,33098823]
["PLA2G6","phospholipase A2 group VI","HGNC:9039","PLA2G6 Deficiency Sensitizes Trophoblasts to Ferroptosis.","_NA_","Human, mice","_NA_","PLA2G6 :-: Ferroptosis","Validated","_NA_","_NA_",null,33087576]
["MIR424","microRNA 424","HGNC:31881","In the present study, we show that miR-424-5p negatively regulates ferroptosis by directly targeting ACSL4 in ovarian cancer cells.","_NA_","Human","_NA_","miR-424-5p :-: ACSL4, ACSL4 :+: Ferroptosis","Validated","_NA_","_NA_",null,33038905]
["PARK7","Parkinsonism associated deglycase","HGNC:16369","Therefore, our results further implied that the C terminus of DJ-1 mediates the suppression of ferroptosis, and when the C-terminal structure is destroyed, DJ-1 inhibition of ferroptosis is abolished.","_NA_","Human, mice","_NA_","PARK7 :-: Ferroptosis","Validated","_NA_","_NA_",null,33024240]
["FXN","frataxin","HGNC:3951","Most importantly, frataxin deficiency enhanced ferroptosis driven by ethanol via evaluating the levels of lactate dehydrogenase, cell morphological changes, mitochondrial labile iron pool, and lipid peroxidation. Conversely, restoring frataxin alleviated the sensitivity to ferroptosis. In addition, frataxin overexpression mitigated the sensitivity of ethanol-induced ferroptosis in HepG2CYP2E1+/+.","_NA_","Human","_NA_","FXN :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","_NA_",null,33017621]
["SUV39H1","SUV39H1 histone lysine methyltransferase","HGNC:11479","Mechanistically, we discovered that siRNA knockdown or pharmacological inhibition of SUV39H1 induced iron accumulation and lipid peroxidation, leading to ferroptosis that disrupted ccRCC cell growth in vitro and in vivo.","_NA_","Human","_NA_","SUV39H1 :-: DPP4, DPP4 :+: Ferroptosis","Validated","_NA_","_NA_",null,33643820]
["ATF2","activating transcription factor 2","HGNC:784","All these results suggested that ATF2 inhibited BETi-induced ferroptosis by increasing NRF2 expression.","_NA_","Human","_NA_","ATF2 :+: NRF2, NRF2 :-: Ferroptosis","Validated","_NA_","_NA_",null,33008584]
["CDKN1A","cyclin dependent kinase inhibitor 1A","HGNC:1784","While this study was performed in cancer cell lines, our results support the potential of p21 to aid in maintenance of healthy tissues by blocking the damage incurred due to ferroptosis.","_NA_","Human","_NA_","(p21/CDK2) :-: Ferroptosis","Predicted","_NA_","_NA_",null,32979260]
["FTH1","ferritin heavy chain 1","HGNC:3976","FTH1 Inhibits Ferroptosis Through Ferritinophagy in the 6-OHDA Model of Parkinson's Disease.","_NA_","Rat","_NA_","FTH1 :-: Ferroptosis","Validated","_NA_","_NA_",null,32959272]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Nrf2 Upregulation Inhibits Ferroptosis and Plays a Protective Role in IIR-ALI.","_NA_","Mice","_NA_","Nrf2 :+ SLC7A11, SLC7A11 :+: Cystine, Cystine :+: GSH, GSH :+: GPX4, GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","_NA_",null,33014271]
["STAT3","signal transducer and activator of transcription 3","HGNC:11364","STAT3 Overexpression Upregulates SLC7A11 and Ameliorates OGD/R-Induced MLE12 Ferroptosis.","_NA_","Mice","_NA_","STAT3 :+ SLC7A11, SLC7A11 :+: Cystine, Cystine :+: GSH, GSH :+: GPX4, GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","_NA_",null,33014271]
["ACOT1","acyl-CoA thioesterase 1","HGNC:33128","Overexpression of Acot1 in cell lines showed noteworthy protection to ferroptosis, while the knock-down of Acot1 sensitized cardiomyocytes to ferroptosis by DIC.","_NA_","Mice","_NA_","ACOT1 :-: Ferroptosis","Validated","_NA_","_NA_",null,32934217]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Furthermore, Nfe2l2 (Nrf2) knockdown sensitized cells to ferroptotic cell death.","_NA_","Rat","_NA_","Nrf2 :+: SLC7A11, SLC7A11 :+: GSH, GSH :-: Ferroptosis, Nrf2 :+: GCL, GCL :+: GSH, GSH :-: Ferroptosis, Nrf2 :+: FTH1, FTH1 :-: Ferroptosis","Validated","_NA_","_NA_",null,32937103]
["ALDH3A2","aldehyde dehydrogenase 3 family member A2","HGNC:403","Thus, our results show that loss of Aldh3a2 induces ferroptotic cell death in leukemic cells and is synthetically lethal with GPX4 inhibtion.","_NA_","Mice","_NA_","ALDH3A2 :-: Ferroptosis","Validated","_NA_","_NA_",null,32458004]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Nrf2 acivation inhibited seawater-induced ferroptosis in MLE-12 cells. Nrf2 deficiency aggravated lung injury and ferroptosis in mice induced by seawater drowning.","_NA_","Mice","_NA_","Nrf2 :-: Ferroptosis","Validated","_NA_","_NA_",null,32907551]
["STK11","serine/threonine kinase 11","HGNC:11389","Loss of function of the tumor suppressor LKB1 enhances sensitivity to ferroptosis in human non-small cell lung cancer cells.","_NA_","Human","_NA_","LKB1 :+: AMPK, AMPK :-: ACC1, ACC1 :+: FAS, FAS :+: Ferroptosis","Validated","_NA_","_NA_",null,32883948]
["FNDC5","fibronectin type III domain containing 5","HGNC:20240","Serum irisin levels are decreased and negatively correlated with disease severity in patients with sepsis, and irisin treatment suppresses ferroptosis and restores mitochondrial function in experimental sepsis.","_NA_","Human, mice","_NA_","Irisin :-: Ferroptosis","Deduced","_NA_","_NA_",null,32997405]
["CircIL4R","_NA_","_NA_","CircIL4R knockdown impeded oncogenesis and expedited ferroptosis of HCC cells.","_NA_","Human","_NA_","CircIL4R :-: miR-541-3p, miR-541-3p :-: GPX4, GPX4 :-: Ferroptosis","Validated","_NA_","_NA_",null,32808701]
["CDH1","cadherin 1","HGNC:1748","CDH1 silencing or ZEB1 overexpression increased the susceptibility to ferroptosis, whereas CDH overexpression or ZEB1 silencing decreased the susceptibility, in vitro and in vivo.","_NA_","Human, mice","_NA_","CDH1 :-: Ferroptosis","Validated","_NA_","_NA_",null,32896720]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","NRF2 negatively regulates FOCAD expression. FOCAD enhances the sensitivity of NSCLC cells to the ferroptosis induced by cysteine deprivation, but not GPX4 inhibition.","_NA_","Human","_NA_","NFE2L2 :-: FOCAD, FOCAD :+: FAK, FAK :+: Ferroptosis","Validated","_NA_","_NA_",null,32898818]
["MIR214","microRNA 214","HGNC:31591","PVT1 silencing or miR-214 overexpression significantly reduced infarct size and suppressed ferroptosis in vivo.","_NA_","Human, mice","_NA_","MIR214 :-: PVT1, PVT1 :+: Ferroptosis","Validated","_NA_","_NA_",null,32827544]
["NEDD4L","NEDD4 like E3 ubiquitin protein ligase","HGNC:7728","Here, we screened 571 UPS-related E1, E2, and E3 genes in a human pancreatic cancer cell line (PANC1) and identified the upregulation of NEDD4-like E3 ubiquitin protein ligase (NEDD4L) as a novel ferroptosis suppressor.","_NA_","Human","_NA_","NEDD4L :-: LTF, LTF :+: Ferroptosis","Validated","_NA_","_NA_",null,32811647]
["SQSTM1","sequestosome 1","HGNC:11280","Taken together, our results indicated that high p62 expression protects SH-SY5Y cells against 6-OHDA-induced ferroptosis.","_NA_","Human","_NA_","(SQSTM1/Keap1) :+: Nrf2, Nrf2 :-: Ferroptosis, (SQSTM1/Keap1) :+: HO-1, HO-1 :-: Ferroptosis","Validated","_NA_","_NA_",null,32770451]
["TF","transferrin","HGNC:11740","Trf-LKO mice display iron-deficiency anemia, iron overload in tissues, and susceptibility to liver damage via ferroptosis.","_NA_","Human, mice","_NA_","TF :-: Ferroptosis","Validated","_NA_","_NA_",null,32374849]
["FTMT","ferritin mitochondrial","HGNC:17345","FTMT and ferritin heavy chain (FTH) cooperated to protect macrophages from RSL-3-induced ferroptosis under hypoxia as this form of cell death is linked to iron metabolism. In hypoxic macrophages either FTMT or FTH was sufficient to pro\u0002tect from ferroptosis","_NA_","Human","_NA_","FTMT :-: Ferroptosis","Validated","ferritin mitochondrial","Q8N4E7","FTMT_HUMAN",32810738]
["BRD2","bromodomain containing 2\n","HGNC:1103","Inhibition of BET and the proteasome triggers ferroptosis cell death.","_NA_","Human","_NA_","BRD2 :-: Ferroptosis","Deduced","Bromodomain-containing protein 2","P25440","BRD2_HUMAN",32937365]
["BRD3","bromodomain containing 3","HGNC:1104","Inhibition of BET and the proteasome triggers ferroptosis cell death.","_NA_","Human","_NA_","BRD3 :-: Ferroptosis","Deduced","Bromodomain-containing protein 3","Q15059?(BRD3_HUMAN",null,32937365]
["BRD4","bromodomain containing 4","HGNC:13575","Inhibition of BET and the proteasome triggers ferroptosis cell death.","_NA_","Human","_NA_","BRD4 :-: Ferroptosis","Deduced","Bromodomain-containing protein 4","O60885","BRD4_HUMAN",32937365]
["BRDT","bromodomain testis associated","HGNC:1105","Inhibition of BET and the proteasome triggers ferroptosis cell death.","_NA_","Human","_NA_","BRDT :-: Ferroptosis","Deduced","Bromodomain testis-specific protein","Q58F21","BRDT_HUMAN",32937365]
["SCD","Stearoyl-CoA desaturase","HGNC:10571","Collectively, this study demonstrated that SCD1 could promote tumor growth of gastric cancer cells and protect them from ferroptotic cell death.","_NA_","Human, mice","_NA_","SCD :+: SLC7A11, SLC7A11 :-: Ferroptosis, SCD :+: GPX4, GPX4 :-: Ferroptosis","Validated","Acyl-CoA desaturase","O00767","ACOD_HUMAN",32726752]
["SLC7A11","solute carrier family 7 member 11","HGNC:11059","Furthermore, we found that SLC7A11 knockdown increased vulnerability of mature MO3.13 cells to ferroptosis.","_NA_","Human","_NA_","SLC7A11 :-: Ferroptosis","Validated","_NA_","_NA_",null,32608563]
["DECR1","2,4-dienoyl-CoA reductase 1","HGNC:2753","Collectively, these results suggest that DECR1 expression protect cells from oxidative stress and that DECR1 knockdown-induced cell death is primarily mediated by induction of ferroptosis.","_NA_","Human","_NA_","DECR1 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing], mitochondrial","Q16698","DECR_HUMAN",32686647]
["NFE2L2","NFE2 like bZIP transcription factor 2","HGNC:7782","Nrf2 inhibition promotes erastin?induced ferroptosis.","_NA_","Human","_NA_","NFE2L2 :+: GSH, GSH :-: Ferroptosis","Validated","Nuclear factor erythroid 2-related factor 2","Q16236","NF2L2_HUMAN",32642794]
["PTGS2","Prostaglandin-endoperoxide synthase 2","HGNC:9605","Simply a downstream marker of ferroptosis. The most upregulated gene in BJeLR cells upon treatment with either erastin or (1S, 3R)-RSL3, but ferroptotic cell death was not affected by inhibition of the enzyme.","RT-qPCR, cell viability","Human","BJeLR cells","Validated","Prostaglandin G/H synthase 2","P35354","PGH2_HUMAN",24439385]
["DUSP1","Dual specificity phosphatase 1","HGNC:3064","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Dual specificity protein phosphatase 1","P28562","DUS1_HUMAN",24439385]
["NOS2","Nitric oxide synthase 2","HGNC:7873","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Nitric oxide synthase","P35228","NOS2_HUMAN",24439385]
["NCF2","Neutrophil cytosolic factor 2","HGNC:7661","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Neutrophil cytosol factor 2","P19878","NCF2_HUMAN",24439385]
["MT3","Metallothionein 3","HGNC:7408","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Metallothionein-3","P25713","MT3_HUMAN",24439385]
["UBC","Ubiquitin C","HGNC:12468","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Polyubiquitin-C","P0CG48","UBC_HUMAN",24439385]
["ALB","Albumin","HGNC:399","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Serum albumin","P02768","ALBU_HUMAN",24439385]
["TXNRD1","Thioredoxin reductase 1","HGNC:12437","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Thioredoxin reductase 1","Q16881","TRXR1_HUMAN",24439385]
["SRXN1","Sulfiredoxin 1","HGNC:16132","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Sulfiredoxin-1","Q9BYN0","SRXN1_HUMAN",24439385]
["GPX2","Glutathione peroxidase 2","HGNC:4554","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Glutathione peroxidase 2","P18283","GPX2_HUMAN",24439385]
["BNIP3","BCL2 interacting protein 3","HGNC:1084","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","BCL2/adenovirus E1B 19 kDa protein-interacting protein 3","Q12983","BNIP3_HUMAN",24439385]
["OXSR1","Oxidative stress responsive kinase 1","HGNC:8508","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Serine/threonine-protein kinase OSR1","O95747","OXSR1_HUMAN",24439385]
["SELENOS","Selenoprotein S","HGNC:30396","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Selenoprotein S","Q9BQE4","SELS_HUMAN",24439385]
["ANGPTL7","Angiopoietin like 7","HGNC:24078","Expression was downregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Angiopoietin-related protein 7","O43827","ANGL7_HUMAN",24439385]
["CHAC1","ChaC glutathione specific gamma-glutamylcyclotransferase 1","HGNC:28680","Up-regulated in erastin-treated samples.  A useful pharmacodynamic marker of system Xc- inhibition.","RNA-seq, RT-qPCR","Human","HT-1080 and Calu-1 cells","Validated","Glutathione-specific gamma-glutamylcyclotransferase 1","Q9BUX1","CHAC1_HUMAN",24844246]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Similar to erastin treatment, silencing of this gene inhibits glutamate release. Erastin specifically inhibits SLC7A11-dependent system Xc- function.","RNA silencing, RT-qPCR","Human","HT-1080 cells","Deduced","Cystine/glutamate transporter","Q9UPY5","XCT_HUMAN",24844246]
["DDIT4","DNA damage inducible transcript 4","HGNC:24944","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","DNA damage-inducible transcript 4 protein","Q9NX09","DDIT4_HUMAN",24844246]
["LOC284561","_NA_","_NA_","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","_NA_","_NA_",null,24844246]
["ASNS","Asparagine synthetase (glutamine-hydrolyzing)","HGNC:753","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Asparagine synthetase [glutamine-hydrolyzing]","P08243","ASNS_HUMAN",24844246]
["TSC22D3","TSC22 domain family member 3","HGNC:3051","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","TSC22 domain family protein 3","Q99576","T22D3_HUMAN",24844246]
["DDIT3","DNA damage inducible transcript 3","HGNC:2726","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","DDIT3 upstream open reading frame protein","P0DPQ6","DT3UO_HUMAN",24844246]
["JDP2","Jun dimerization protein 2","HGNC:17546","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Jun dimerization protein 2","Q8WYK2","JDP2_HUMAN",24844246]
["SESN2","Sestrin 2","HGNC:20746","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Sestrin-2","P58004","SESN2_HUMAN",24844246]
["SLC1A4","Solute carrier family 1 member 4","HGNC:10942","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Neutral amino acid transporter A","P43007","SATT_HUMAN",24844246]
["PCK2","Phosphoenolpyruvate carboxykinase 2, mitochondrial","HGNC:8725","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Phosphoenolpyruvate carboxykinase [GTP]","Q16822","PCKGM_HUMAN",24844246]
["TXNIP","Thioredoxin interacting protein","HGNC:16952","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Thioredoxin-interacting protein","Q9H3M7","TXNIP_HUMAN",24844246]
["VLDLR","Very low density lipoprotein receptor","HGNC:12698","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Very low-density lipoprotein receptor","P98155","VLDLR_HUMAN",24844246]
["GPT2","Glutamic--pyruvic transaminase 2","HGNC:18062","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Alanine aminotransferase 2","Q8TD30","ALAT2_HUMAN",24844246]
["PSAT1","Phosphoserine aminotransferase 1","HGNC:19129","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Phosphoserine aminotransferase","Q9Y617","SERC_HUMAN",24844246]
["LURAP1L","Leucine rich adaptor protein 1 like","HGNC:31452","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Leucine rich adaptor protein 1-like","Q8IV03","LUR1L_HUMAN",24844246]
["SLC7A5","Solute carrier family 7 member 5","HGNC:11063","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Large neutral amino acids transporter small subunit 1","Q01650","LAT1_HUMAN",24844246]
["HERPUD1","Homocysteine inducible ER protein with ubiquitin like domain 1","HGNC:13744","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein","Q15011","HERP1_HUMAN",24844246]
["XBP1","X-box binding protein 1","HGNC:12801","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","X-box-binding protein 1","P17861","XBP1_HUMAN",24844246]
["ATF3","Activating transcription factor 3","HGNC:785","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Cyclic AMP-dependent transcription factor ATF-3","P18847","ATF3_HUMAN",24844246]
["SLC3A2","Solute carrier family 3 member 2","HGNC:11026","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","4F2 cell-surface antigen heavy chain","P08195","4F2_HUMAN",24844246]
["CBS","Cystathionine beta-synthase","HGNC:1550","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Cystathionine beta-synthase","P35520","CBS_HUMAN",24844246]
["ATF4","Activating transcription factor 4","HGNC:786","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Cyclic AMP-dependent transcription factor ATF-4","P18848","ATF4_HUMAN",24844246]
["ZNF419","Zinc finger protein 419","HGNC:20648","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Zinc finger protein 419","Q96HQ0","ZN419_HUMAN",24844246]
["KLHL24","Kelch like family member 24","HGNC:25947","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Kelch-like protein 24","Q6TFL4","KLH24_HUMAN",24844246]
["TRIB3","Tribbles pseudokinase 3","HGNC:16228","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Tribbles homolog 3","Q96RU7","TRIB3_HUMAN",24844246]
["ZFP69B","ZFP69 zinc finger protein B","HGNC:28053","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Zinc finger protein 69 homolog B","Q9UJL9","ZF69B_HUMAN",24844246]
["ATP6V1G2","ATPase H+ transporting V1 subunit G2","HGNC:862","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","V-type proton ATPase subunit G 2","O95670","VATG2_HUMAN",24844246]
["VEGFA","Vascular endothelial growth factor A","HGNC:12680","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Vascular endothelial growth factor A","P15692","VEGFA_HUMAN",24844246]
["GDF15","Growth differentiation factor 15","HGNC:30142","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Growth/differentiation factor 15","Q99988","GDF15_HUMAN",24844246]
["TUBE1","Tubulin epsilon 1","HGNC:20775","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Tubulin epsilon chain","Q9UJT0","TBE_HUMAN",24844246]
["ARRDC3","Arrestin domain containing 3","HGNC:29263","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Arrestin domain-containing protein 3","Q96B67","ARRD3_HUMAN",24844246]
["CEBPG","CCAAT enhancer binding protein gamma","HGNC:1837","Up-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","CCAAT/enhancer-binding protein gamma","P53567","CEBPG_HUMAN",24844246]
["SNORA16A","Small nucleolar RNA, H/ACA box 16A","HGNC:32605","Down-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","_NA_","_NA_",null,24844246]
["RGS4","Regulator of G protein signaling 4","HGNC:10000","Down-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","Regulator of G-protein signaling 4","P49798","RGS4_HUMAN",24844246]
["BLOC1S5-TXNDC5","BLOC1S5-TXNDC5 readthrough (NMD candidate)","HGNC:42001","Down-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","_NA_","_NA_",null,24844246]
["LOC390705","_NA_","_NA_","Down-regulated (>= 2 fold) in erastin-treated samples.","RNA-seq","Human","HT-1080 cells","Screened","_NA_","_NA_",null,24844246]
["EIF2S1","eukaryotic translation initiation factor 2 subunit alpha","HGNC:3265","Phosphorylated in erastin-treated sample.","Western blot","Human","HT-1080 cells","Deduced","Eukaryotic translation initiation factor 2 subunit 1","P05198","IF2A_HUMAN",24844246]
["KIM-1","Kidney injury molecule-1","_NA_","Down-regulated upon Fer-1 appearance","Real-time quantitative RT-PCR","Mice","Fer-1–treated CaOx model","Deduced","_NA_","_NA_",null,25385600]
["IL6","Interleukin 6","HGNC:6018","Down-regulated upon Fer-1 appearance","Real-time quantitative RT-PCR","Mice","Fer-1–treated CaOx model","Deduced","Interleukin-6","P05231","IL6_HUMAN",25385600]
["CXCL2","C-X-C motif chemokine ligand 2","HGNC:4603","Down-regulated upon Fer-1 appearance","Real-time quantitative RT-PCR","Mice","Fer-1–treated CaOx model","Deduced","C-X-C motif chemokine 2","P19875","CXCL2_HUMAN",25385600]
["RELA","RELA proto-oncogene, NF-kB subunit","HGNC:9955","Down-regulated upon Fer-1 appearance","Real-time quantitative RT-PCR","Mice","Fer-1–treated CaOx model","Deduced","Transcription factor p65","Q04206","TF65_HUMAN",25385600]
["HSD17B11","Hydroxysteroid 17-beta dehydrogenase 11","HGNC:22960","Enriched in RSL3-resistant cells.","Gene trap insertion","Human","Chronic myeloid leukemia cell line KBM7","Screened","Estradiol 17-beta-dehydrogenase 11","Q8NBQ5","DHB11_HUMAN",25965523]
["AGPAT3","1-acylglycerol-3-phosphate O-acyltransferase 3","HGNC:326","Enriched in RSL3-resistant cells.","Gene trap insertion","Human","Chronic myeloid leukemia cell line KBM7","Screened","1-acyl-sn-glycerol-3-phosphate acyltransferase gamma","Q9NRZ7","PLCC_HUMAN",25965523]
["SETD1B","SET domain containing 1B, histone lysine methyltransferase","HGNC:29187","Enriched in GPX4 inhibitor ML162-resistant cells.","Gene trap insertion","Human","Chronic myeloid leukemia cell line KBM7","Screened","Histone-lysine N-methyltransferase SETD1B","Q9UPS6","SET1B_HUMAN",25965523]
["HMOX1","Heme oxygenase 1","HGNC:5013","Its expression increased in response to artesunate-induced ferroptosis, indicating activation of ROS-mediated signaling pathways.","Western blotting","Human","Pancreatic adenocarcinoma cell line Panc-1 cells","Deduced","Heme oxygenase 1","P09601","HMOX1_HUMAN",26097885]
["TF","Transferrin","HGNC:11740","Its expression is decreased in patients.","Gene expression analysis","Human","Patient PDAC tissues","Deduced","Serotransferrin","P02787","TRFE_HUMAN",26097885]
["FTL","Ferritin light chain","HGNC:3999","Its expression  is decreased in patients.","Gene expression analysis","Human","Patient PDAC tissues","Deduced","Ferritin light chain","P02792","FRIL_HUMAN",26097885]
["RPL8","Ribosomal protein L8","HGNC:10368","Significantly reduced in tumor tissues","Gene expression analysis","Human","Patient PDAC tissues","Deduced","60S ribosomal protein L8","P62917","RL8_HUMAN",26097885]
["ATP5MC3","ATP synthase membrane subunit c locus 3","HGNC:843","Significantly reduced in tumor tissues","Gene expression analysis","Human","Patient PDAC tissues","Deduced","ATP synthase F(0) complex subunit C3","P48201","AT5G3_HUMAN",26097885]
["TFRC","Transferrin receptor","HGNC:11763","Expression of this gene is increased in patients.","Gene expression analysis","Human","Patient PDAC tissues","Deduced","Transferrin receptor protein 1","P02786","TFR1_HUMAN",26097885]
["MAFG","MAF bZIP transcription factor G","HGNC:6781","The interaction between NRF2 and MafG was increased in response to erastin and sorafenib.","Western blot","Human, mice","HepG2, Hepa1‐6, Hep3B, and SNU‐182 cells","Deduced","Transcription factor MafG","O15525","MAFG_HUMAN",26403645]
["IL33","Interleukin 33","HGNC:16028","IL-33 upregulation is a feature of ferroptosis. Ferrostatin-1, an inhibitor of ferroptosis, prevented the upregulation of IL-33.","Western blot, quantitative PCR, ELISA","Mice","C57BL/6 mice","Deduced","Interleukin-33","O95760","IL33_HUMAN",27352622]
["FTH1","Ferritin heavy chain 1","HGNC:3976","An increase of endogenous FTH1 level during ferroptosis. Degradation of FTH1 protein upon ferroptosis induction.","Immunoblotting, q-PCR","Human","HT1080 cells","Validated","Ferritin heavy chain","P02794","FRIH_HUMAN",27514700]
["SLC40A1","Solute carrier family 40 member 1","HGNC:10909","Erastin-induced mRNA expression is upregulated in FANCD2-deficient cells.","Q-PCR","Mice","Bone marrow stromal cells","Validated","Solute carrier family 40 member 1","Q9NP59","S40A1_HUMAN",27773819]
["TF","Transferrin","HGNC:11740","Erastin-induced mRNA expression is upregulated in FANCD2-deficient cells.","Q-PCR","Mice","Bone marrow stromal cells","Validated","Serotransferrin","P02787","TRFE_HUMAN",27773819]
["TFRC","Transferrin receptor","HGNC:11763","Erastin-induced mRNA expression is upregulated in FANCD2-deficient cells.","Q-PCR","Mice","Bone marrow stromal cells","Validated","Transferrin receptor protein 1","P02786","TFR1_HUMAN",27773819]
["FTH1","Ferritin heavy chain 1","HGNC:3976","Erastin-induced mRNA expression is downregulated in FANCD2-deficient cells.","Q-PCR, western blot","Mice","Bone marrow stromal cells","Validated","Ferritin heavy chain","P02794","FRIH_HUMAN",27773819]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Erastin-induced mRNA expression is downregulated in FANCD2-deficient cells.","Q-PCR, western blot","Mice","Bone marrow stromal cells","Validated","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",27773819]
["HAMP","Hepcidin antimicrobial peptide","HGNC:15598","Erastin-induced mRNA expression is downregulated in FANCD2-deficient cells.","Q-PCR","Mice","Bone marrow stromal cells","Deduced","Hepcidin","P81172","HEPC_HUMAN",27773819]
["HSPB1","Heat shock protein family B (small) member 1","HGNC:5246","Erastin-induced mRNA expression is downregulated in FANCD2-deficient cells.","Q-PCR","Mice","Bone marrow stromal cells","Validated","Heat shock protein beta-1","P04792","HSPB1_HUMAN",27773819]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Erastin-induced mRNA expression is downregulated in FANCD2-deficient cells.","Q-PCR","Mice","Bone marrow stromal cells","Validated","Nuclear factor erythroid 2-related factor 2","Q16236","NF2L2_HUMAN",27773819]
["STEAP3","STEAP3 metalloreductase","HGNC:24592","Erastin-induced mRNA expression is downregulated in FANCD2-deficient cells.","Q-PCR","Mice","Bone marrow stromal cells","Deduced","Metalloreductase STEAP3","Q658P3","STEA3_HUMAN",27773819]
["DRD5","Dopamine receptor D5","HGNC:3026","Ferroptotic erastin induces DRD5 gene expression in ferroptosis.","Western blot, Q-PCR","Human","PANC1 cells","Deduced","D(1B) dopamine receptor","P21918","DRD5_HUMAN",27793671]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Erastin promoted GPX4 degradation. Antiferroptotic dopamine increased the protein stability of glutathione peroxidase 4.","Western blot, Q-PCR","Human","PANC1 cells","Deduced","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",27793671]
["DRD4","Dopamine receptor D4","HGNC:3025","Antiferroptotic dopamine suppressed dopamine receptor D4 protein degradation. Ferroptotic erastin promotes DRD4 protein degradation.","Western blot, Q-PCR","Human","PANC1 cells","Deduced","D(4) dopamine receptor","P21917","DRD4_HUMAN",27793671]
["MAP3K5","Mitogen-activated protein kinase kinase kinase 5","HGNC:6857","Cold stress evokes ferroptosis, and the ASK1‐p38 pathway is activated downstream of lipid peroxide, leading to the cell death. ASK1‐p38 axis is also activated in the erastin‐induced ferroptosis model.","LDH assay, immunoblot, RNAi, CCK-8","Human","A549 cells","Deduced","Mitogen-activated protein kinase kinase kinase 5","Q99683","M3K5_HUMAN",28887319]
["MAPK14","Mitogen-activated protein kinase 14","HGNC:6876","Cold stress evokes ferroptosis, and the ASK1‐p38 pathway is activated downstream of lipid peroxide, leading to the cell death. ASK1‐p38 axis is also activated in the erastin‐induced ferroptosis model.","LDH assay, immunoblot, RNAi, CCK-8","Human","A549 cells","Deduced","Mitogen-activated protein kinase 14","Q16539","MK14_HUMAN",28887319]
["SLC2A1","Solute carrier family 2 member 1","HGNC:11005","Increased at LSH overexpression. Decreased at LSH knockdown. LSH can inhibit ferroptosis.","ChIP analysis, RT-qPCR","Human","H358, A549 cells","Deduced","Solute carrier family 2","P11166","GTR1_HUMAN",28900510]
["SLC2A3","Solute carrier family 2 member 3","HGNC:11007","Decreased at LSH knockdown. LSH can inhibit ferroptosis.","RT-qPCR","Human","H358, A549 cells","Deduced","Solute carrier family 2","P11169","GTR3_HUMAN",28900510]
["SLC2A6","Solute carrier family 2 member 6","HGNC:11011","Increased at LSH overexpression. Decreased at LSH knockdown. LSH can inhibit ferroptosis.","RT-qPCR","Human","H358, A549 cells","Deduced","Solute carrier family 2","Q9UGQ3","GTR6_HUMAN",28900510]
["SLC2A8","Solute carrier family 2 member 8","HGNC:13812","Decreased at LSH knockdown. LSH can inhibit ferroptosis.","RT-qPCR","Human","H358, A549 cells","Deduced","Solute carrier family 2","Q9NY64","GTR8_HUMAN",28900510]
["SLC2A12","Solute carrier family 2 member 12","HGNC:18067","Increased at LSH overexpression. Decreased at LSH knockdown. LSH can inhibit ferroptosis.","RT-qPCR","Human","H358, A549 cells","Deduced","Solute carrier family 2","Q8TD20","GTR12_HUMAN",28900510]
["GLUT13","_NA_","_NA_","Increased at LSH overexpression. Decreased at LSH knockdown. LSH can inhibit ferroptosis.","RT-qPCR","Human","H358, A549 cells","Deduced","_NA_","_NA_",null,28900510]
["SLC2A14","Solute carrier family 2 member 14","HGNC:18301","Decreased at LSH knockdown. LSH can inhibit ferroptosis.","RT-qPCR","Human","H358, A549 cells","Deduced","Solute carrier family 2","Q8TDB8","GTR14_HUMAN",28900510]
["EIF2AK4","Eukaryotic translation initiation factor 2 alpha kinase 4","HGNC:19687","CHAC1 degradation of GSH enhances cystine-starvation-induced ferroptosis through the activated GCN2-eIF2 alpha-ATF4 pathway.","Western blot, real-time PCR, cell viability assay, siRNA, GSH assay kit","Human","MDA-MB-231, Hs 578T, and HCC 1937 cells","Deduced","eIF-2-alpha kinase GCN2","Q9P2K8","E2AK4_HUMAN",29383104]
["EIF2S1","eukaryotic translation initiation factor 2 subunit alpha","HGNC:3265","CHAC1 degradation of GSH enhances cystine-starvation-induced ferroptosis through the activated GCN2-eIF2 alpha-ATF4 pathway.","Western blot, real-time PCR, cell viability assay, siRNA, GSH assay kit","Human","MDA-MB-231, Hs 578T, and HCC 1937 cells","Deduced","Eukaryotic translation initiation factor 2 subunit 1","P05198","IF2A_HUMAN",29383104]
["ATF4","Activating transcription factor 4","HGNC:786","CHAC1 degradation of GSH enhances cystine-starvation-induced ferroptosis through the activated GCN2-eIF2 alpha-ATF4 pathway.","Western blot, real-time PCR, cell viability assay, siRNA, GSH assay kit","Human","MDA-MB-231, Hs 578T, and HCC 1937 cells","Deduced","Cyclic AMP-dependent transcription factor ATF-4","P18848","ATF4_HUMAN",29383104]
["ALOX5","Arachidonate 5-lipoxygenase","HGNC:435","Overexpression sensitizes cells to ferroptosis.","Western blot, mass spectrometry, cell viability assay, gene overexpression","Human","HEK293 cells","Deduced","Arachidonate 5-lipoxygenase","P09917","LOX5_HUMAN",29632885]
["ALOX12","Arachidonate 12-lipoxygenase, 12S type","HGNC:429","Overexpression sensitizes cells to ferroptosis.","Western blot, mass spectrometry, cell viability assay, gene overexpression","Human","HEK293 cells","Deduced","Arachidonate 12-lipoxygenase","P18054","LOX12_HUMAN",29632885]
["ALOX15","Arachidonate 15-lipoxygenase","HGNC:433","Overexpression sensitizes cells to ferroptosis.","Western blot, mass spectrometry, cell viability assay, gene overexpression","Human","HEK293 cells","Deduced","Arachidonate 15-lipoxygenase","P16050","LOX15_HUMAN",29632885]
["ALOX5","Arachidonate 5-lipoxygenase","HGNC:435","Necessary for hemin‐induced ferroptosis in vitro.","qRT-PCR, immunoblotting, MTT assay","Mice","C57BL/6 and pregnant CD1 mice, primary cortical neurons","Deduced","Arachidonate 5-lipoxygenase","P09917","LOX5_HUMAN",30294906]
["ACSF2","Acyl-CoA synthetase family member 2","HGNC:26101","Increased in ferroptotic events. Decreased in DFO-induced resistance to ferroptosis.","Western blot, glutathione detection, RT-qPCR, immunofluorescence staining","Rat","Wistar rats","Deduced","Medium-chain acyl-CoA ligase ACSF2","Q96CM8","ACSF2_HUMAN",30539824]
["IREB2","Iron responsive element binding protein 2","HGNC:6115","Increased in ferroptotic events. Decreased in DFO-induced resistance to ferroptosis.","Western blot, glutathione detection, RT-qPCR, immunofluorescence staining","Rat","Wistar rats","Deduced","Iron-responsive element-binding protein 2","P48200","IREB2_HUMAN",30539824]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Upregulated in DFO-induced resistance to ferroptosis.","Western blot, glutathione detection, RT-qPCR, immunofluorescence staining","Rat","Wistar rats","Validated","Phospholipid hydroperoxide glutathione peroxidase","P36969","GPX4_HUMAN",30539824]
["HMGB1","High mobility group box 1","HGNC:4983","Associated with ferroptotic cell death. Ferroptosis activators induce HMGB1 release.","ELISA, western blot, RNAi","Human, mice","HT1080 and PANC1 cells, mouse embryonic fibroblasts","Deduced","High mobility group protein B1","P09429","HMGB1_HUMAN",30686534]
["HMOX1","Heme oxygenase 1","HGNC:5013","Required for DOX-induced ferroptosis.","RNA-seq, qRT-PCR, HIS","Mice","Mice","Deduced","Heme oxygenase 1","P09601","HMOX1_HUMAN",30692261]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","DOX treatment induced ferroptosis. Protein and mRNA levels of Nrf2 were increased after DOX treatment.","RNA-seq, QuantiChrom Heme Assay Kit, iron staining, qRT-PCR, survival curve, HIS","Mice","Mice","Deduced","Nuclear factor erythroid 2-related factor 2","Q16236","NF2L2_HUMAN",30692261]
["ELAVL1","ELAV like RNA binding protein 1","HGNC:3312","Binds to and increases the expression of the negative ferroptosis regulator LINC00336.","RNA pulldown, mass spectrometry, qRT-PCR, gene overexpression, gene knockdown, western blot","Human","H358, PC9, SPC-A-1, and A549 cells","Deduced","ELAV-like protein 1","Q15717","ELAV1_HUMAN",30787392]
["SLC3A2","Solute carrier family 3 member 2","HGNC:11026","Strongly correlated with resistance to ferroptosis inducers.","Western blot, cell viability assay, cell viability assay, MDA assay, glutamate assay","Human","HT-1080 cells","Deduced","4F2 cell-surface antigen heavy chain","P08195","4F2_HUMAN",31043744]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Strongly correlated with resistance to ferroptosis inducers.","Western blot, cell viability assay, cell viability assay, MDA assay, glutamate assay","Human","HT-1080 cells","Deduced","Cystine/glutamate transporter","Q9UPY5","XCT_HUMAN",31043744]
["TFAP2C","Transcription factor AP-2 gamma","HGNC:11744","Activated by ferroptosis inhibitor selenium.","Behavioral analysis, infarct volume analysis, immunocytochemistry, TUNEL staining, fluoro-jade Staining, qRT-PCR, cell death assay, ChIP assay, siRNA, western blot, immunoblotting, RNA-seq, mass spectrometry","Mice","C57BL/6 mice, primary cortical neurons, HT22 murine hippocampal cells","Deduced","Transcription factor AP-2 gamma","Q92754","AP2C_HUMAN",31056284]
["SP1","Sp1 transcription factor","HGNC:11205","Activated by ferroptosis inhibitor selenium.","Behavioral analysis, infarct volume analysis, immunocytochemistry, TUNEL staining, fluoro-jade Staining, qRT-PCR, cell death assay, ChIP assay, siRNA, western blot, immunoblotting, RNA-seq, mass spectrometry","Mice","C57BL/6 mice, primary cortical neurons, HT22 murine hippocampal cells","Deduced","Transcription factor Sp1","P08047","SP1_HUMAN",31056284]
["HBA1","Hemoglobin subunit alpha 1","HGNC:4823","Upregulated in cells treated with ferroptosis inducer erastin. Stimulates ferroptosis possibly in a GSH-dependent manner.","Tandem mass tags, RNA-seq, DNase-seq, qPCR, Western blot, IHC","Human","HepG2, Bel-7402 cells","Deduced","Hemoglobin subunit alpha","P69905","HBA_HUMAN",31108460]
["NNMT","Nicotinamide N-methyltransferase","HGNC:7861","Upregulated in cells treated with ferroptosis inducer erastin. Stimulates ferroptosis possibly in a GSH-dependent manner.","Tandem mass tags, RNA-seq, DNase-seq, qPCR, Western blot, IHC","Human","HepG2, Bel-7402 cells","Deduced","Nicotinamide N-methyltransferase","P40261","NNMT_HUMAN",31108460]
["PLIN4","Perilipin 4","HGNC:29393","Upregulated in cells treated with ferroptosis inducer erastin. Stimulates ferroptosis possibly in a GSH-dependent manner.","Tandem mass tags, RNA-seq, DNase-seq, qPCR, Western blot, IHC","Human","HepG2, Bel-7402 cells","Deduced","Perilipin-4","Q96Q06","PLIN4_HUMAN",31108460]
["HIC1","HIC ZBTB transcriptional repressor 1","HGNC:4909","Essential for stimulation of pro-ferroptotic gene transcription upon ferroptosis induction. ","Immunohistochemistry, immunofluorescence, western blot, qPCR, metabolites examination, ChIP","Human","HepG2, Bel-7402 cells","Deduced","Hypermethylated in cancer 1 protein","Q14526","HIC1_HUMAN",31108460]
["STMN1","Stathmin 1","HGNC:6510","Downregulated in cells treated with ferroptosis inducer erastin. Suppresses ferroptosis possibly in a GSH-dependent manner.","Tandem mass tags, RNA-seq, DNase-seq, qPCR, Western blot, IHC","Human","HepG2, Bel-7402 cells","Deduced","Stathmin","P16949","STMN1_HUMAN",31108460]
["RRM2","Ribonucleotide reductase regulatory subunit M2","HGNC:10452","Downregulated in cells treated with ferroptosis inducer erastin. Suppresses ferroptosis possibly in a GSH-dependent manner.","Tandem mass tags, RNA-seq, DNase-seq, qPCR, Western blot, IHC","Human","HepG2, Bel-7402 cells","Deduced","Ribonucleoside-diphosphate reductase subunit M2","P31350","RIR2_HUMAN",31108460]
["CAPG","Capping actin protein, gelsolin like","HGNC:1474","Downregulated in cells treated with ferroptosis inducer erastin. Suppresses ferroptosis possibly in a GSH-dependent manner.","Tandem mass tags, RNA-seq, DNase-seq, qPCR, Western blot, IHC","Human","HepG2, Bel-7402 cells","Deduced","Macrophage-capping protein","P40121","CAPG_HUMAN",31108460]
["HNF4A","Hepatocyte nuclear factor 4 alpha","HGNC:5024","Essential for stimulation of anti-ferroptotic gene transcription. ","Immunohistochemistry, immunofluorescence, western blot, qPCR, metabolites examination, ChIP","Human","HepG2, Bel-7402 cells","Deduced","Hepatocyte nuclear factor 4-alpha","P41235","HNF4A_HUMAN",31108460]
["NGB","Neuroglobin","HGNC:14077","Human neuroglobin (hNgb)-EGFP-expressing SH-SY5Y cells to be significantly more resistant to ferroptosis induction.","Cell death, cell viability, lipid peroxidation assay, RT-qPCR","Human","SH-SY5Y cells","Deduced","Neuroglobin","Q9NPG2","NGB_HUMAN",31405213]
["YWHAE","Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon","HGNC:12851","Required by RSL3 (a ferroptosis inducer) to inactivate GPX4 (a ferroptosis inhibitor).","Mass spectrometry, siRNA, western blot","Human","HEK293T cells","Deduced","14-3-3 protein epsilon","P62258","1433E_HUMAN",31581313]
["GABPB1","GA binding protein transcription factor subunit beta 1","HGNC:4074","Downregulated by lncRNA GABPB1-AS1 upon erastin treatment.","RT-qPCR, western blot, siRNA, cell viability, lipid peroxidation assay","Human","HepG2, Huh7, and Hep3B cells","Deduced","GA-binding protein subunit beta-1","Q06547","GABP1_HUMAN",31700067]
["AURKA","Aurora kinase A","HGNC:11393","Inhibition of AURKA or reconstitution of miR-4715-3p inhibited GPX4 and induced cell death, suggesting a link between AURKA and ferroptosis.","qRT-PCR, western blot, lentiviral infection, CellTiter-Glo luminescence assay","Human","OE33, STKM2, MKN45 cells","Predicted","Aurora kinase A","O14965","AURKA_HUMAN",31740746]
["MIR4715","microRNA 4715","HGNC:41666","Inhibition of AURKA or reconstitution of miR-4715-3p inhibited GPX4 and induced cell death, suggesting a link between AURKA and ferroptosis.","qRT-PCR, western blot, lentiviral infection, CellTiter-Glo luminescence assay","Human","OE33, STKM2, MKN45 cells","Predicted","_NA_","_NA_",null,31740746]
["RIPK1","Receptor interacting serine/threonine kinase 1","HGNC:10019","Reduced expression results in resistance to ferroptosis.","Cell viability, western blot, RNA-seq, GSH assay, qRT-PCR, immunofluorescence imaging","Human","Patient-derived fibroblasts","Deduced","Receptor-interacting serine/threonine-protein kinase 1","Q13546","RIPK1_HUMAN",31827280]
["PRDX1","Peroxiredoxin 1","HGNC:9352","Necessary to ferroptosis-related lipid peroxidation.","Western blot, siRNA, flow cytometry, cell viability, RT-PCR","Human","Primary corneal endothelial cells, B4G12 and HT1080 cells","Deduced","Peroxiredoxin-1","Q06830","PRDX1_HUMAN",31901729]
["MIR30B","microRNA 30b","HGNC:31625","Upregulation of miR-30b-5p in preeclampsia models plays a pivotal role in ferroptosis.","GSH assay, MDA measurement, western blot, immunohistochemistry, microarray, RT-qPCR, labile iron assay, cell viability, LDH assay","Human, rat","Trophoblasts, preeclampsia model, HTR-8/SVneo and TEV-1 cells","Deduced","_NA_","_NA_",null,31926626]
["MMP13","matrix metallopeptidase 13","HGNC:7159","Moreover, erastin, the most classic inducer of ferroptosis, promoted matrix metalloproteinase 13 (MMP13) expression while inhibited type II collagen (collagen II) expression in chondrocytes.","Gene expression analysis","Human","chondrocytes","Deduced",{"$nulls":3},33376672]
["LRRFIP1","LRR binding FLII interacting protein 1","HGNC:6702","Furthermore, LRRFIP1 levels were increased by pro-oxidant conditions such as ODG or the ferroptosis inducer erastin.","Co-Immunoprecipitation Assays, LC-MS/MS, Immunocytochemistry","Rat","pregnant rats","Deduced",{"$nulls":3},33265962]
["RPL8","Ribosomal protein L8","HGNC:10368","Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis.","shRNA screening, qPCR, gene silencing","Human","NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell","RPL8 :+: Ferroptosis","Validated","60S ribosomal protein L8","P62917","RL8_HUMAN",22632970]
["IREB2","iron responsive element binding protein 2","HGNC:6115","Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis.","shRNA screening, qPCR, gene silencing","Human","NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell","IREB2 :+: Ferroptosis","Validated","Iron-responsive element-binding protein 2","P48200","IREB2_HUMAN",22632970]
["ATP5MC3","ATP synthase membrane subunit c locus 3","HGNC:843","Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis.","shRNA screening, qPCR, gene silencing","Human","NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell","ATP5MC3 :+: Ferroptosis","Validated","ATP synthase F(0) complex subunit C3","P48201","AT5G3_HUMAN",22632970]
["CS","Citrate synthase","HGNC:2422","Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis.","shRNA screening, qPCR, gene silencing","Human","NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell","CS :+: Ferroptosis","Validated","Citrate synthase","O75390","CISY_HUMAN",22632970]
["EMC2","ER membrane protein complex subunit 2","HGNC:28963","Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis.","shRNA screening, qPCR, gene silencing","Human","NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell","EMC2 :+: Ferroptosis","Validated","ER membrane protein complex subunit 2","Q15006","EMC2_HUMAN",22632970]
["ACSF2","Acyl-CoA synthetase family member 2","HGNC:26101","Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis.","shRNA screening, qPCR, gene silencing","Human","NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell","ACSF2 :+: Ferroptosis","Validated","Medium-chain acyl-CoA ligase ACSF2","Q96CM8","ACSF2_HUMAN",22632970]
["NOX1","Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) 1","HGNC:7889","Suppresses erastin-induced ferroptosis when inhibited by inhibitor.","Inhibition test by diphenylene iodonium (DPI) and GKT137831.","Human","KRAS mutant Calu-1 non small cell lung cancer cell","NOX1 :+: Ferroptosis","Deduced","NADPH oxidase 1","Q9Y5S8","NOX1_HUMAN",22632970]
["CYBB","Cytochrome b-245 beta chain","HGNC:2578","Suppresses erastin-induced ferroptosis when inhibited by inhibitor.","Inhibition test by diphenylene iodonium (DPI).","Human","KRAS mutant Calu-1 non small cell lung cancer cell","CYBB :+: Ferroptosis","Deduced","Cytochrome b-245 heavy chain","P04839","CY24B_HUMAN",22632970]
["NOX3","Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) 3","HGNC:7890","Suppresses erastin-induced ferroptosis when inhibited by inhibitor.","Inhibition test by diphenylene iodonium (DPI).","Human","KRAS mutant Calu-1 non small cell lung cancer cell","NOX3 :+: Ferroptosis","Deduced","NADPH oxidase 3","Q9HBY0","NOX3_HUMAN",22632970]
["NOX4","Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) 4","HGNC:7891","Suppresses erastin-induced ferroptosis when inhibited by inhibitor.","Inhibition test by diphenylene iodonium (DPI) and GKT137831.","Human","KRAS mutant Calu-1 non small cell lung cancer cell","NOX4 :+: Ferroptosis","Deduced","NADPH oxidase 4","Q9NPH5","NOX4_HUMAN",22632970]
["NOX5","Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) 5","HGNC:14874","Suppresses erastin-induced ferroptosis when inhibited by inhibitor.","Inhibition test by diphenylene iodonium (DPI).","Human","KRAS mutant Calu-1 non small cell lung cancer cell","NOX5 :+: Ferroptosis","Deduced","NADPH oxidase 5","Q96PH1","NOX5_HUMAN",22632970]
["DUOX1","Dual oxidase 1","HGNC:3062","Suppresses erastin-induced ferroptosis when inhibited by inhibitor.","Inhibition test by diphenylene iodonium (DPI).","Human","KRAS mutant Calu-1 non small cell lung cancer cell","DUOX1 :+: Ferroptosis","Deduced","Dual oxidase 1","Q9NRD9","DUOX1_HUMAN",22632970]
["DUOX2","Dual oxidase 2","HGNC:13273","Suppresses erastin-induced ferroptosis when inhibited by inhibitor.","Inhibition test by diphenylene iodonium (DPI).","Human","KRAS mutant Calu-1 non small cell lung cancer cell","DUOX2 :+: Ferroptosis","Deduced","Dual oxidase 2","Q9NRD8","DUOX2_HUMAN",22632970]
["G6PD","Glucose-6-phosphate dehydrogenase","HGNC:4057","Required in erastin-induced ferroptosis.","shRNA silencing, RT-qPCR","Human","KRAS mutant Calu-1 non small cell lung cancer cell","G6PD :+: Ferroptosis","Validated","Glucose-6-phosphate 1-dehydrogenase","P11413","G6PD_HUMAN",22632970]
["PGD","Phosphoglycerate dehydrogenase","HGNC:8891","Required in erastin-induced ferroptosis.","shRNA silencing, RT-qPCR","Human","KRAS mutant Calu-1 non small cell lung cancer cell","PGD :+: Ferroptosis","Validated","6-phosphogluconate dehydrogenase","P52209","6PGD_HUMAN",22632970]
["VDAC2","Valtage-dependent anion channels 2","HGNC:12672","Required in erastin-induced ferroptosis.","shRNA silencing, RT-qPCR","Human","KRAS mutant Calu-1 non small cell lung cancer cell","VDAC2 :+: Ferroptosis","Validated","Voltage-dependent anion-selective channel protein 2","P45880","VDAC2_HUMAN",22632970]
["PIK3CA","Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha","HGNC:8975","Inhibitor blocked ferroptotic cell death.","Cell survival assays","Mice","HT22 cells","PIK3CA :-: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Phosphatidylinositol 4","P42336","PK3CA_HUMAN",24739485]
["FLT3","Fms related tyrosine kinase 3","HGNC:3765","Inhibitor blocked ferroptotic cell death.","Cell survival assays","Mice","HT22 cells","FLT3 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Receptor-type tyrosine-protein kinase FLT3","P36888","FLT3_HUMAN",24739485]
["SCP2","Sterol carrier protein 2","HGNC:10606","SCP-2 inhibitors showed anti-ferroptotic activity, which was substantiated by knocking down SCP-2 in Gpx4?/? cells.","Cell viability test, siRNA-mediated knockdown.","Mice","PZL cells","SCP2 :+: Ferroptosis","Validated","Non-specific lipid-transfer protein","P22307","NLTP_HUMAN",25402683]
["TP53","Tumor protein p53","HGNC:11998","Inhibits cystine uptake and sensitizes cells to ferroptosis. Erastin induced high levels of cell death in p53+/+ MEFs.","Cell death kinetics, shRNA, western blotting, RT-PCR, ChIP assay, cystine uptake assay","Human, mice","H1299, U2OS and MCF7 cells, mouse embryonic fibroblasts","TP53 :-: SLC7A11, SLC7A11 :+: Cystine, Cystine :-: Ferroptosis","Validated","Cellular tumor antigen p53","P04637","P53_HUMAN",25799988]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Deletion of this gene likely suppress ferroptosis by limiting the membrane-resident pool of oxidation-sensitive fatty acids.","Retroviral-mediated insertional mutagenesis and sequencing","Human","Chronic myeloid leukemia cell line KBM7","ACSL4 :+: Ferroptosis","Predicted","Long-chain-fatty-acid--CoA ligase 4","O60488","ACSL4_HUMAN",25965523]
["LPCAT3","Lysophosphatidylcholine acyltransferase 3","HGNC:30244","Deletion of this gene likely suppress ferroptosis by limiting the membrane-resident pool of oxidation-sensitive fatty acids.","Retroviral-mediated insertional mutagenesis and sequencing","Human","Chronic myeloid leukemia cell line KBM7","LPCAT3 :+: Ferroptosis","Predicted","Lysophospholipid acyltransferase 5","Q6P1A2","MBOA5_HUMAN",25965523]
["NRAS","NRAS proto-oncogene, GTPase","HGNC:7989","NRAS12V mutant protects RMS13 cells from ferroptotic cell death.","Cell viability assessed by MTT assay, western blot","Human","RMS13 cells","NRAS :+: Ferroptosis","Deduced","GTPase NRas","P01111","RASN_HUMAN",26157704]
["KRAS","KRAS proto-oncogene, GTPase","HGNC:6407","KRAS12V mutant protects RMS13 cells from ferroptotic cell death.","Cell viability assessed by MTT assay, western blot","Human","RMS13 cells","KRAS :+: Ferroptosis","Deduced","GTPase KRas","P01116","RASK_HUMAN",26157704]
["HRAS","HRas proto-oncogene, GTPase","HGNC:5173","HRAS12V mutant protects RMS13 cells from ferroptotic cell death.","Cell viability assessed by MTT assay, western blot","Human","RMS13 cells","HRAS :+: Ferroptosis","Deduced","GTPase HRas","P01112","RASH_HUMAN",26157704]
["TF","Transferrin","HGNC:11740","Essential for the induction of ferroptotic cell death. Transferrin can only interact with transferrin receptor and be transported into the cell when it is loaded with iron.","Killing activity","Mice","Mouse embryonic fibroblasts","TF :+: Ferroptosis","Validated","Serotransferrin","P02787","TRFE_HUMAN",26166707]
["TFRC","Transferrin receptor","HGNC:11763","RNAi of transferrin receptor (TfR) inhibited ferroptosis.","RNAi","Mice","Mouse embryonic fibroblasts","TFRC :+: Ferroptosis","Validated","Transferrin receptor protein 1","P02786","TFR1_HUMAN",26166707]
["TFR2","Transferrin receptor 2","HGNC:11762","RNAi of transferrin receptor (TfR) inhibited ferroptosis.","RNAi","Mice","Mouse embryonic fibroblasts","TFR2 :+: Ferroptosis","Validated","Transferrin receptor protein 2","Q9UP52","TFR2_HUMAN",26166707]
["SLC38A1","Solute carrier family 38 member 1","HGNC:13447","RNAi knockdown markedly blocked ferroptosis.","RNAi knockdown, cell survival analysis","Mice","Mouse embryonic fibroblasts","SLC38A1 :+: Glutamine, Glutamine :+: Glutamate, Glutamate :+: alpha-Ketoglutarate, alpha-Ketoglutarate :+: Ferroptosis","Validated","Sodium-coupled neutral amino acid transporter 1","Q9H2H9","S38A1_HUMAN",26166707]
["SLC1A5","Solute carrier family 1 member 5","HGNC:10943","Pharmacological inhibition by L-g-glutamyl-p-nitroanilide or RNAi knockdown markedly blocked ferroptosis.","RNAi knockdown, cell survival analysis","Mice","Mouse embryonic fibroblasts","SLC1A5 :+: Glutamine, Glutamine :+: Glutamate, Glutamate :+: alpha-Ketoglutarate, alpha-Ketoglutarate :+: Ferroptosis","Validated","Neutral amino acid transporter B(0)","Q15758","AAAT_HUMAN",26166707]
["GLS2","Glutaminase 2","HGNC:29570","Both inhibitor inhibition and gene knockdown inhibit ferroptotic cell death.","shRNA, western blot, cell viability","Mice","Mouse embryonic fibroblasts","GSL2 :+: Glutamate, Glutamate :+: Ferroptosis","Validated","Glutaminase liver isoform","Q9UI32","GLSL_HUMAN",26166707]
["GOT1","Glutamic-oxaloacetic transaminase 1","HGNC:4432","RNAi reduced ferroptosis.","shRNA, qPCR, cell viability","Mice","Mouse embryonic fibroblasts","GOT1 :+: alpha-Ketoglutarate, alpha-Ketoglutarate :+: Ferroptosis","Validated","Aspartate aminotransferase","P17174","AATC_HUMAN",26166707]
["CARS1","Cysteinyl-tRNA synthetase 1","HGNC:1493","Required for ferroptosis in diverse cell contexts. Knockdown of CARS inhibited erastin-induced death by preventing the induction of lipid reactive oxygen species, without altering iron homeostasis.","siRNA screen, shRNA, western blot, cell viability","Human, rat","HT-1080 fibrosarcoma cells,BJeLR fibroblast cells, Panc1 cells, A673 (Ewing’s sarcoma) and 143B (osteosarcoma) cells, PC12 cells","CARS1 :-: Transsulfuration pathway, Transsulfuration pathway :-: Ferroptosis","Validated","Cysteine--tRNA ligase","P49589","SYCC_HUMAN",26184909]
["TP53","Tumor protein p53","HGNC:11998","Wild type p53 can induce ferroptosis upon reactive oxygen species (ROS)-induced stress.","Cell death quantification, western blot","Human","H1299 cells","TP53 :-: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","Cellular tumor antigen p53","P04637","P53_HUMAN",26218928]
["ALOX5","Arachidonate 5-lipoxygenase","HGNC:435","The 5-Lipoxygenase inhibitor zileuton protected HT22 neuronal cells from erastin-induced ferroptosis.","Cell Viability Assay, ROS measurement","Mice","HT22 mouse neuronal cell line","ALOX5 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Arachidonate 5-lipoxygenase","P09917","LOX5_HUMAN",26235588]
["KEAP1","Kelch like ECH associated protein 1","HGNC:23177","Knockdown of Keap1 reversed loss of p62-increased degradation of NRF2 in ferroptosis. Keap1 knockdown led to resistance to erastin-induced and sorafenib-induced growth inhibition with decreased ferroptotic events in the absence or presence of p62 knockdown.","Western blot, shRNA knockdown, cell viability","Human, mice","HepG2, Hepa1‐6, Hep3B, and SNU‐182 cells","KEAP1 :-: NFE2L2, NFE2L2 :-: Ferroptosis","Validated","Kelch-like ECH-associated protein 1","Q14145","KEAP1_HUMAN",26403645]
["HMOX1","Heme oxygenase 1","HGNC:5013","Zinc protoporphyrin IX, a HO-1 inhibitor, prevented Erastin-triggered ferroptotic cancer cell death. Overexpression accelerates erastin-induced cell death.","Cell viability assay, qRT-PCR, western blot","Human, mice","HT-1080 fibrosarcoma cells, mice lung fibroblastic cells","HMOX1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Heme oxygenase 1","P09601","HMOX1_HUMAN",26405158]
["TP53","Tumor protein p53","HGNC:11998","p53^3KR/3KR Xrcc4^-/- MEF cells are very sensitive to ferroptosis. Stabilized in the spleens of p53^3KR/3KR Xrcc4^-/- mice which enables to prevent the development of pro-B-cell lymphomas.","Western blot, qRT-PCR, cell viability","Mice","p53^3KR/3KR Xrcc4^-/- MEF cells, p53^3KR/3KR Xrcc4^-/- mice","TP53 :-: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","Cellular tumor antigen p53","P04637","P53_HUMAN",26943586]
["TP53","Tumor protein p53","HGNC:11998","Incubation with erastin led to profound cell death in wild-type MEFs.","Cell viability assay, phase-contrast microscopy","Human, mice","Mouse embryonic fibroblasts, lymphoblastoid cell lines","TP53 :-: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","Cellular tumor antigen p53","P04637","P53_HUMAN",27034505]
["GLS2","Glutaminase 2","HGNC:29570","Upregulated in erastin-treated wild-type MEFs, and silencing Gls2 exihibits cell death defect in wild-type MEFs treated with erastin.","Cell viability assay,western blot","Mice","Mouse embryonic fibroblasts","GLS2 :+: Ferroptosis","Validated","Glutaminase liver isoform","Q9UI32","GLSL_HUMAN",27034505]
["ATG5","Autophagy related 5","HGNC:589","Knockout or knockdown limited erastin-induced ferroptosis.","shRNA","Human, mice","Mouse embryonic fibroblasts, human pancreatic cancer cell lines (PANC1 and PANC2.03), and the human fibrosarcoma cell line HT-1080","ATG5 :+: Ferroptosis","Validated","Autophagy protein 5","Q9H1Y0","ATG5_HUMAN",27245739]
["ATG7","Autophagy related 7","HGNC:16935","Knockout or knockdown limited erastin-induced ferroptosis.","shRNA","Human, mice","Mouse embryonic fibroblasts, human pancreatic cancer cell lines (PANC1 and PANC2.03), and the human fibrosarcoma cell line HT-1081","ATG7 :+: Ferroptosis","Validated","Ubiquitin-like modifier-activating enzyme ATG7","O95352","ATG7_HUMAN",27245739]
["NCOA4","Nuclear receptor coactivator 4","HGNC:7671","Inhibition suppressed ferroptosis, and overexpression promoted ferroptosis.","shRNA, gene transfection","Human","PANC1 or HT-1080 cells","NCOA4 :-: FTH1, FTH1 :-: Ferroptosis","Validated","Nuclear receptor coactivator 4","Q13772","NCOA4_HUMAN",27245739]
["TF","Transferrin","HGNC:11740","Involved in siramesine and lapatinib-induced ferroptotic cell death. Increased following treatment with lapatinib alone or in combination with siramesine. Knocking down of transferrin resulted in decreased cell death and ROS after treatment.","Western blot, densitometry quantification, siRNA, gene transfection, cell death test","Human","MDA MB 231 and SKBr3 cells","TF :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Serotransferrin","P02787","TRFE_HUMAN",27441659]
["ALOX5","Arachidonate 5-lipoxygenase","HGNC:435","Silencing ALOX genes made cells resistant to ferroptosis.","qPCR","Human","G-401 cells","ALOX5 :+: PUFAs peroxidation, PUFAs peroxidation :+: Ferroptosis","Validated","Arachidonate 5-lipoxygenase","P09917","LOX5_HUMAN",27506793]
["ALOX12","Arachidonate 12-lipoxygenase, 12S type","HGNC:429","Silencing ALOX genes made cells resistant to ferroptosis.","qPCR","Human","G-401 cells","ALOX12 :+: PUFAs peroxidation, PUFAs peroxidation :+: Ferroptosis","Validated","Arachidonate 12-lipoxygenase","P18054","LOX12_HUMAN",27506793]
["ALOX12B","Arachidonate 12-lipoxygenase, 12R type","HGNC:430","Silencing ALOX genes made cells resistant to ferroptosis.","qPCR","Human","G-401 cells","ALOX12B :+: PUFAs peroxidation, PUFAs peroxidation :+: Ferroptosis","Validated","Arachidonate 12-lipoxygenase","O75342","LX12B_HUMAN",27506793]
["ALOX15","Arachidonate 15-lipoxygenase","HGNC:433","Silencing ALOX genes made cells resistant to ferroptosis.","qPCR","Human","G-401 cells","ALOX15 :+: PUFAs peroxidation, PUFAs peroxidation :+: Ferroptosis","Validated","Arachidonate 15-lipoxygenase","P16050","LOX15_HUMAN",27506793]
["ALOX15B","Arachidonate 15-lipoxygenase type B","HGNC:434","Silencing ALOX genes made cells resistant to ferroptosis. Erastin-induced cell death was rescued by silencing either ALOX15B or ALOXE3, which supported the hypothesis that lipoxygenases are required for ferroptosis.","qPCR, siRNA","Human","G-401, BJeLR and HT-1080 cells","ALOX15B :+: PUFAs peroxidation, PUFAs peroxidation :+: Ferroptosis","Validated","Arachidonate 15-lipoxygenase B","O15296","LX15B_HUMAN",27506793]
["ALOXE3","Arachidonate lipoxygenase 3","HGNC:13743","Silencing ALOX genes made cells resistant to ferroptosis. Erastin-induced cell death was rescued by silencing either ALOX15B or ALOXE3, which supported the hypothesis that lipoxygenases are required for ferroptosis.","qPCR, siRNA","Human","G-401, BJeLR and HT-1080 cells","ALOXE3 :+: PUFAs peroxidation, PUFAs peroxidation :+: Ferroptosis","Validated","Hydroperoxide isomerase ALOXE3","Q9BYJ1","LOXE3_HUMAN",27506793]
["PHKG2","Phosphorylase kinase catalytic subunit gamma 2","HGNC:8931","U-2-OS cells became resistant to erastin upon PHKG2 silencing. Erastin-treated HT-1080 cells were rescued by shPHKG2.","shRNA suppressor screen, qPCR, cellular iron staining, cell viability","Human","U-2-OS, HT-1080 cells","PHKG2 :+: PUFAs peroxidation, PUFAs peroxidation :+: Ferroptosis","Validated","Phosphorylase b kinase gamma catalytic chain","P15735","PHKG2_HUMAN",27506793]
["TFRC","Transferrin receptor","HGNC:11763","The gene targets of the enriched shRNAs are potential genes that positively regulate ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","TFRC :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","Transferrin receptor protein 1","P02786","TFR1_HUMAN",27514700]
["ACO1","Aconitase 1","HGNC:117","The gene targets of the enriched shRNAs are potential genes that positively regulate ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","ACO1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","Cytoplasmic aconitate hydratase","P21399","ACOC_HUMAN",27514700]
["IREB2","iron responsive element binding protein 2","HGNC:6115","The gene targets of the enriched shRNAs are potential genes that positively regulate ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","IREB2 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","Iron-responsive element-binding protein 2","P48200","IREB2_HUMAN",27514700]
["SLC38A1","Solute carrier family 38 member 1","HGNC:13447","The gene targets of the enriched shRNAs are potential genes that positively regulate ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","SLC38A1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","Sodium-coupled neutral amino acid transporter 1","Q9H2H9","S38A1_HUMAN",27514700]
["GLS2","Glutaminase 2","HGNC:29570","The gene targets of the enriched shRNAs are potential genes that positively regulate ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","GLS2 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","Glutaminase liver isoform","Q9UI32","GLSL_HUMAN",27514700]
["G6PDX","_NA_","_NA_","The gene targets of the enriched shRNAs are potential genes that positively regulate ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","G6PDX :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","_NA_","_NA_",null,27514700]
["ULK1","Unc-51 like autophagy activating kinase 1","HGNC:12558","Potential positive regulators of ferroptosis. Knockout of ULK1 led to significantly lower levels of erastin-induced ferroptosis in a dose- and time-dependent manner.","RNAi screening, gene knockout","Mice","Mouse embryonic fibroblasts","ULK1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Serine/threonine-protein kinase ULK1","O75385","ULK1_HUMAN",27514700]
["ATG3","Autophagy related 3","HGNC:20962","Potential positive regulators of ferroptosis. Knockout of ATG3 greatly reduced the sensitivity of MEFs to ferropotosis, and reconstituting ATG3 back to these cells restored the ferroptosis sensitivity.","RNAi screening, gene knockout","Mice","Mouse embryonic fibroblasts","ATG3 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Ubiquitin-like-conjugating enzyme ATG3","Q9NT62","ATG3_HUMAN",27514700]
["ATG4D","Autophagy related 4D cysteine peptidase","HGNC:20789","Potential positive regulators of ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","ATG4D :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","Cysteine protease ATG4D","Q86TL0","ATG4D_HUMAN",27514700]
["ATG5","Autophagy related 5","HGNC:589","Potential positive regulators of ferroptosis. Knockout of ATG5 led to significantly lower levels of erastin-induced ferroptosis in a dose- and time-dependent manner.","RNAi screening","Mice","Mouse embryonic fibroblasts","ATG5 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Autophagy protein 5","Q9H1Y0","ATG5_HUMAN",27514700]
["BECN1","Beclin 1","HGNC:1034","Potential positive regulators of ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","BECN1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","Beclin-1","Q14457","BECN1_HUMAN",27514700]
["MAP1LC3A","Microtubule associated protein 1 light chain 3 alpha","HGNC:6838","Potential positive regulators of ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","MAP1LC3A :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","Microtubule-associated proteins 1A/1B light chain 3A","Q9H492","MLP3A_HUMAN",27514700]
["GABARAPL2","GABA type A receptor associated protein like 2","HGNC:13291","Potential positive regulators of ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","GABARAPL2 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","Gamma-aminobutyric acid receptor-associated protein-like 2","P60520","GBRL2_HUMAN",27514700]
["GABARAPL1","GABA type A receptor associated protein like 1","HGNC:4068","Potential positive regulators of ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","GABARAPL1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","Gamma-aminobutyric acid receptor-associated protein-like 1","Q9H0R8","GBRL1_HUMAN",27514700]
["ATG16L1","Autophagy related 16 like 1","HGNC:21498","Potential positive regulators of ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","ATG16L1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","Autophagy-related protein 16-1","Q676U5","A16L1_HUMAN",27514700]
["WIPI1","WD repeat domain, phosphoinositide interacting 1","HGNC:25471","Potential positive regulators of ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","WIPI1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","WD repeat domain phosphoinositide-interacting protein 1","Q5MNZ9","WIPI1_HUMAN",27514700]
["WIPI2","WD repeat domain, phosphoinositide interacting 2","HGNC:32225","Potential positive regulators of ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","WIPI2 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","WD repeat domain phosphoinositide-interacting protein 2","Q9Y4P8","WIPI2_HUMAN",27514700]
["SNX4","Sorting nexin 4","HGNC:11175","Potential positive regulators of ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","SNX4 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","Sorting nexin-4","O95219","SNX4_HUMAN",27514700]
["ATG13","Autophagy related 13","HGNC:29091","Knockout of ATG13 greatly reduced the sensitivity of MEFs to ferropotosis, and reconstituting ATG13 back to these cells restored the ferroptosis sensitivity.","Gene knockout","Mice","Mouse embryonic fibroblasts","ATG13 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Autophagy-related protein 13","O75143","ATG13_HUMAN",27514700]
["ULK2","Unc-51 like autophagy activating kinase 2","HGNC:13480","Knockout of ULK2 led to significantly lower levels of erastin-induced ferroptosis in a dose- and time-dependent manner.","Gene knockout","Mice","Mouse embryonic fibroblasts","ULK2 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Serine/threonine-protein kinase ULK2","Q8IYT8","ULK2_HUMAN",27514700]
["NCOA4","Nuclear receptor coactivator 4","HGNC:7671","Elimination of NCOA4 expression by RNAi knockdown significantly block ferroptosis","RNAi knockdown, western blot","Human, mice","Mouse embryonic fibroblasts and HT1080 cells","NCOA4 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Nuclear receptor coactivator 4","Q13772","NCOA4_HUMAN",27514700]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Knockdown inhibited erastin-induced ferroptosis, whereas overexpression restored ferroptosis sensitization.","shRNA, gene transfection, western blot","Human","LNCaP (human prostate cancer cells), K562 (human erythromyeloblastoid leukemia cell line), HepG2 (human hepatocellular cells), and HL60 (human promyelocytic leukemia cells)","ACSL4 :+: 5-HETE, 5-HETE :+: Ferroptosis","Validated","Long-chain-fatty-acid--CoA ligase 4","O60488","ACSL4_HUMAN",27565726]
["TP53","Tumor protein p53","HGNC:11998","p53 acetylation has a critical role in ferroptotic responses. Simultaneous loss of K98/117/161/162 acetylations is unable to induce ferroptosis, and its ability to thwart cancer growth is also abrogated.","Mass spectrometry screening, western blot, cell death assay","Human, mice","H1299 cells, mouse xenograft models","TP53 :-: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","Cellular tumor antigen p53","P04637","P53_HUMAN",27705786]
["SAT1","Spermidine/spermine N1-acetyltransferase 1","HGNC:10540","p53-mediated activation of SAT1 contributes to ferroptotic cell death in the presence of ROS stress. Knockdown of Sat1 partially rescued ROS-induced ferroptosis.","qRT-PCR, siRNA, CRISPR-cas9, western blot","Human, mice","H1299, U2OS cells, mouse embryonic fibroblasts, mouse xenograft","SAT1 :+: ALOX15, ALOX15 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Diamine acetyltransferase 1","P21673","SAT1_HUMAN",27698118]
["ALOX15","Arachidonate 15-lipoxygenase","HGNC:433","SAT1- and ROS-induced ferroptosis was completely abrogated by PD146176, an ALOX15-specific inhibitor.","Cell death count, qRT-PCR, siRNA","Human","H1299, U2OS cells","ALOX15 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Arachidonate 15-lipoxygenase","P16050","LOX15_HUMAN",27698118]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Inhibition of ACSL4 was effective in protecting against RSL3-induced cell death. Acsl4 KO cells are resistant to ferroptosis.","Cell death count, LC-MS/MS,  fluorescence imaging","Mice","Mouse embryonic fibroblasts (Pfa1 cells)","ACSL4 :+: PE, PE :+: Ferroptosis","Validated","Long-chain-fatty-acid--CoA ligase 4","O60488","ACSL4_HUMAN",27842066]
["LPCAT3","Lysophosphatidylcholine acyltransferase 3","HGNC:30244","Knockdown of Lpcat3 increased resistance to ferroptosis triggered by RSL3.","shRNA","Mice","Mouse lung epithelial (MLE) and embryonic cells","LPCAT3 :+: Ferroptosis","Validated","Lysophospholipid acyltransferase 5","Q6P1A2","MBOA5_HUMAN",27842066]
["ALOX15","Arachidonate 15-lipoxygenase","HGNC:433","Liproxstatin-1 inhibited the 15-LOX enzymatic activity and suppressed ferroptosis.","Identification of oxygenated PE","Mice","Kidney of GPX4-deficient mice","ALOX15 :+: PE, PE :+: Ferroptosis","Validated","Arachidonate 15-lipoxygenase","P16050","LOX15_HUMAN",27842066]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","An essential proferroptotic gene. Re-expression of Flag-tagged human wild-type (WT) ACSL4 (ACSL4-Flag) in Acsl4 KO Pfa1 cells restored full sensitivity to ferroptosis induction. Inhibition showed significantly prolonged survival compared to vehicle-treated mice.","Cell viability, immunoblot,  lipid peroxidation assessment, CRISPR–Cas9, qPCR, microarray, RNA-seq","Human, mice","Mouse embryonic fibroblasts (Pfa1 cells), human breast cancer cell lines, Gpx4 KO mice","ACSL4 :+: Ferroptosis","Validated","Long-chain-fatty-acid--CoA ligase 4","O60488","ACSL4_HUMAN",27842070]
["KEAP1","Kelch like ECH associated protein 1","HGNC:23177","Keap 1 silencing decreased ferroptosis.","Cell viability assay, western blot, siRNA","Mice","Head and neck cancer cells, nude mice","KEAP1 :-: NFE2L2, NFE2L2 :-: Ferroptosis","Validated","Kelch-like ECH-associated protein 1","Q14145","KEAP1_HUMAN",28012440]
["EGFR","Epidermal growth factor receptor","HGNC:3236","Cell death in activated EGFR mutant cells occurs by ferroptosis. Inhibiting EGFR and MAPK signaling rescued cell viability following cystine withdrawal.","Fluorescence-activated cell sorting (FACS) analysis, cell viability","Human","Human mammary epithelial) cells","EGFR :+: MAPK, MAPK :+: Ferroptosis","Validated","Epidermal growth factor receptor","P00533","EGFR_HUMAN",28297659]
["NOX4","NADPH oxidase 4","HGNC:7891","Inhibition of NADPH oxidase 4 (NOX4) blocked ferroptosis.","Fluorescence-activated cell sorting (FACS) analysis, cell viability","Human","Human mammary epithelial) cells","NOX4 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","NADPH oxidase 4","Q9NPH5","NOX4_HUMAN",28297659]
["MAPK3","Mitogen-activated protein kinase 3","HGNC:6877","Inhibiting EGFR and MAPK signaling rescued cell viability following cystine withdrawal.","Immunoblots, lucifer yellow infiltration, cell viability, fluorescence-activated cell sorting (FACS) analysis","Human","Human mammary epithelial) cells","MAPK :-: GPX4, GPX4 :-: Lipid ROS, MAPK :+: NOX4, NOX4 :+: H2O2, H2O2 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Mitogen-activated protein kinase 3","P27361","MK03_HUMAN",28297659]
["MAPK1","Mitogen-activated protein kinase 1","HGNC:6871","Inhibiting EGFR and MAPK signaling rescued cell viability following cystine withdrawal.","Immunoblots, lucifer yellow infiltration, cell viability, fluorescence-activated cell sorting (FACS) analysis","Human","Human mammary epithelial) cells","MAPK :-: GPX4, GPX4 :-: Lipid ROS, MAPK :+: NOX4, NOX4 :+: H2O2, H2O2 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Mitogen-activated protein kinase 1","P28482","MK01_HUMAN",28297659]
["BID","BH3 interacting domain death agonist","HGNC:1050","BID deletion prevents erastin- and glutamate-induced cell death. BID inhibition inhibited erastin-induced ferroptosis.","Cell viability, western blot, CRISPR/Cas9, gene transfection","Mice","HT-22 cells, mouse embryonic fibroblasts","BID :+: Ferroptosis","Validated","BH3-interacting domain death agonist","P55957","BID_HUMAN",28384611]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Knockout of Acsl4 in ferroptosis-sensitive cells conferred protection from erastin- and RSL3-induced cell death.","CRISPR/Cas9, western blotting, cell death assessment","Human, mice","NIH3T3, HT-29, HT-1080 and L929 cells","ACSL4 :+: Ferroptosis","Validated","Long-chain-fatty-acid--CoA ligase 4","O60488","ACSL4_HUMAN",28551825]
["ZEB1","Zinc finger E-box binding homeobox 1","HGNC:11642","Knockout of ZEB1 prevents cell death induced by GPX4 inhibition.","sgRNA, cell viability","Human","KP4 pancreatic cancer cells","ZEB1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Zinc finger E-box-binding homeobox 1","P37275","ZEB1_HUMAN",28678785]
["KEAP1","Kelch like ECH associated protein 1","HGNC:23177","Keap1 inhibition promotes resistance to ferroptosis.","Cell viability analysis","Human, rat","F98, U87 glioma cells","KEAP1 :+: Ferroptosis","Validated","Kelch-like ECH-associated protein 1","Q14145","KEAP1_HUMAN",28805788]
["DPP4","Dipeptidyl peptidase 4","HGNC:3009","Required for ferroptosis in TP53-deficient CRC cells.","Cell death, RNAi, western blot, enzyme activity","Human, mice","TP53-/- HCT116 cells, athymic nude mice","DPP4 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Dipeptidyl peptidase 4","P27487","DPP4_HUMAN",28813679]
["ALOX15","Arachidonate 15-lipoxygenase","HGNC:433","Suppression of ferroptosis following ALOX15 silencing was detected in cancer cells. Cells with exogenous expression of ALOX15 had an increased cell death rate following RSL3 treatment.","siRNA, transfection, western blot, cellular lipoxygenase assay","Human","HT1080, Panc‐1, Calu‐1 cells","ALOX15 :+: Ferroptosis","Validated","Arachidonate 15-lipoxygenase","P16050","LOX15_HUMAN",28837253]
["ALOX12","Arachidonate 12-lipoxygenase, 12S type","HGNC:429","12‐LOX inhibitors prevented cell death, whereas ALOX12 overexpression significantly enhanced cell death. ALOX12 expression was gradually elevated during the erastin or RSL3 treatments, and was stable in the late stage of ferroptosis.","siRNA, transfection, western blot, cellular lipoxygenase assay","Human","HT1080, Panc‐1, Calu‐1 cells","ALOX12 :+: Ferroptosis","Validated","Arachidonate 12-lipoxygenase","P18054","LOX12_HUMAN",28837253]
["CDKN2A","Cyclin dependent kinase inhibitor 2A","HGNC:1787","Combination of ARF induction and ROS treatment induced ferroptotic cell death. Knockdown of endogenous ARF protected cells from ROS-induced cell death.","RNAi, immunoblot, phase-contrast image, cell death quantification","Human, mice","H1299, SaoS2, U2OS cells, mouse embryonic fibroblasts","CDKN2A :-: NFE2L2, NFE2L2 :+: SLC7A11, SLC7A11 :-: Ferroptosis, CDKN2A :+: p53, p53 :-: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","Cyclin-dependent kinase inhibitor 2A","P42771","CDN2A_HUMAN",28985506]
["PEBP1","Phosphatidylethanolamine binding protein 1","HGNC:8630","Elevated levels of PEBP1 resulted in increased sensitivity of HK2 cells to RSL3 whereas lowered contents of PEBP1 in HAEC and HT22 cells were associated with decreased sensitivity to ferroptosis.","Western blot, siRNA, cell death assay","Human, mice","HK2, HAEC and HT22 cells","(PEBP1/15LO) :+: Ferroptosis","Validated","Phosphatidylethanolamine-binding protein 1","P30086","PEBP1_HUMAN",29053969]
["SOCS1","Suppressor of cytokine signaling 1","HGNC:19383","Expression of SOCS1 sensitized cells to ferroptosis inducer. This effect of SOCS1 was efficiently blocked by ferroptosis inhibitor. Expression of SOCS1 reduced the levels of GSH, explaining in part its ability to sensitize cells to ferroptosis.","Cell death assay, cell viability assay, qPCR","Human","U2OS or IMR90 cells","SOCS1 :+: p53, p53 :-: SLC7A11, SLC7A11 :+: GSH, GSH :-: Ferroptosis","Validated","Suppressor of cytokine signaling 1","O15524","SOCS1_HUMAN",29081404]
["CDO1","Cysteine dioxygenase type 1","HGNC:1795","CDO1 suppression contributes to ferroptosis resistance.","siRNA, western blot, MTT assay","Human","Gastric cancer cells AGS, BGC823, athymic nude mice","CDO1 :-: GPX4, GPX4 :-: Ferroptosis","Validated","Cysteine dioxygenase type 1","Q16878","CDO1_HUMAN",29144989]
["MYB","MYB proto-oncogene, transcription factor","HGNC:7545","Erastin-induced ferroptosis was restrained when c-Myb was suppressed.","Western blot, siRNA, qRT-PCR, MTT assay","Human","Gastric cancer cells AGS, BGC823, MKN45, SGC7901, and MGC803","MYB :+: CDO1, CDO1 :-: GPX4, GPX4 :-: Ferroptosis","Validated","Transcriptional activator Myb","P10242","MYB_HUMAN",29144989]
["HMOX1","Heme oxygenase 1","HGNC:5013","Inhibiting HO-1 effectively attenuated BAY-induced ferroptotic cell death. Defective HO-1 expression significantly rescued cell survival suppressed by BAY.","Cell viability assay, qRT-PCR, shRNA, gene overexpression, western blot","Human","MDA-MB-231, MDA-MB-468","HMOX1 :+: Ferroptosis","Validated","Heme oxygenase 1","P09601","HMOX1_HUMAN",29274359]
["MAPK8","Mitogen-activated protein kinase 8","HGNC:6881","JNK1/2 inhibitors inhibited t-BHP-induced ferroptosis. t-BHP treatment significantly increased the protein expression of p-JNK.","MitoSOX probe, luminometer kit, C11-BODIPY probe, western blot","Rat","PC12 cells","MAPK8 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Mitogen-activated protein kinase 8","P45983","MK08_HUMAN",29330409]
["MAPK9","Mitogen-activated protein kinase 9","HGNC:6886","JNK1/2 inhibitors inhibited t-BHP-induced ferroptosis. t-BHP treatment significantly increased the protein expression of p-JNK.","MitoSOX probe, luminometer kit, C11-BODIPY probe, western blot","Rat","PC12 cells","MAPK9 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Mitogen-activated protein kinase 9","P45984","MK09_HUMAN",29330409]
["MAPK1","Mitogen-activated protein kinase 1","HGNC:6871","ERK1/2 inhibitors inhibited t-BHP-induced ferroptosis. t-BHP treatment significantly increased the protein expression of p-ERK.","MitoSOX probe, luminometer kit, C11-BODIPY probe, western blot","Rat","PC12 cells","MAPK1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Mitogen-activated protein kinase 1","P28482","MK01_HUMAN",29330409]
["MAPK3","Mitogen-activated protein kinase 3","HGNC:6877","ERK1/2 inhibitors inhibited t-BHP-induced ferroptosis. t-BHP treatment significantly increased the protein expression of p-ERK.","MitoSOX probe, luminometer kit, C11-BODIPY probe, western blot","Rat","PC12 cells","MAPK3 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Mitogen-activated protein kinase 3","P27361","MK03_HUMAN",29330409]
["SLC1A5","Solute carrier family 1 member 5","HGNC:10943","Overexpression of SLC1A5 restored miR-137-mediated ferroptosis suppression.","Cell viability assay, lipid ROS assay, western blot, gene overexpression, shRNA","Human","Melanoma cell lines A375 and G-361","SLC1A5 :+: Glutaminolysis, Glutaminolysis :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Neutral amino acid transporter B(0)","Q15758","AAAT_HUMAN",29348676]
["CHAC1","ChaC glutathione specific gamma-glutamylcyclotransferase 1","HGNC:28680","CHAC1 degradation of GSH might enhance cystine-starvation-induced cell death.","Western blot, real-time PCR, cell viability assay, siRNA, GSH assay kit","Human","MDA-MB-231, Hs 578T, and HCC 1937 cells","CHAC1 :-: GSH, GSH :-: Ferroptosis","Validated","Glutathione-specific gamma-glutamylcyclotransferase 1","Q9BUX1","CHAC1_HUMAN",29383104]
["MAPK14","Mitogen-activated protein kinase 14","HGNC:6876","Ferroptosis was blocked by inhibiting p38 MAPK activation.","Cell viability assay, lipid ROS measurement, RT-qPCR, western blot","Mice","TM4 Sertoli cells","MAPK14 :+: Ferroptosis","Validated","Mitogen-activated protein kinase 14","Q16539","MK14_HUMAN",29436589]
["LINC00472","Long intergenic non-protein coding RNA 472","HGNC:21380","Increases erastin-induced growth inhibition, whereas depletion of P53RRA decreased erastin-induced growth inhibition.","Bisulfite sequencing, RNA pull-down, mass spectrometry, RNA immunoprecipitation, qRT-PCR, cell viability assay","Human","Lung cancer A549, SPCA1, and H522 cells","LINC00472 :+: p53, p53 :+: Ferroptosis","Validated","Putative uncharacterized protein encoded by LINC00472","Q9H8W2","CF155_HUMAN",29588351]
["NOX4","NADPH oxidase 4","HGNC:7891","Activated  Nox4  contributes to  PAB-induced  ferroptotic cell death. knockdown made cells resistant to PAB-induced death.","Western blot, LDH release assay, siRNA, cell death, siRNA","Human, rat","Rat C6, human SHG-44, U87 and U251 glioma cells","NOX4 :+: H2O2, H2O2 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","NADPH oxidase 4","Q9NPH5","NOX4_HUMAN",29702192]
["GOT1","Glutamic-oxaloacetic transaminase 1","HGNC:4432","Overexpression of miR‐9 suppressed GOT1, which subsequently reduced ferroptosis. Overexpression of GOT1 restored miR‐9 mediated ferroptosis suppression.","Immunoblotting, luciferase reporter assay, qPCR, cell viability assay, MDA assay, iron assay, glutamate assay","Human","A375 and G‐361 cells","GOT1 :+: alpha KG, alpha KG :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Aspartate aminotransferase","P17174","AATC_HUMAN",30035324]
["BECN1","Beclin 1","HGNC:1034","Knockdown inhibits ferroptosis. Overexpression increases ferroptotic cancer cell death.","shRNA, western blot, cell viability assay, gene overexpression","Human, mice","HCT116, CX-1, PANC1, HT1080, and Calu-1 cells, athymic nude immunodeficient mice","BECN1 :-: System Xc-, System Xc- :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Beclin-1","Q14457","BECN1_HUMAN",30057310]
["PRKAA2","Protein kinase AMP-activated catalytic subunit alpha 2","HGNC:9377","Inhibition of PRKAA/AMPKalpha diminishes ferroptosis.","siRNA, western blot, cell viability assay, GSH assay, lipid peroxidation assay, iron assay","Human","HCT116 and CX-1 cells","PRKAA2 :+: (BECN1/SLC7A11), (BECN1/SLC7A11) :+: Ferroptosis","Validated","5'-AMP-activated protein kinase catalytic subunit alpha-2","P54646","AAPK2_HUMAN",30057310]
["PRKAA1","Protein kinase AMP-activated catalytic subunit alpha 1","HGNC:9376","Inhibition of PRKAA/AMPKalpha diminishes ferroptosis.","siRNA, western blot, cell viability assay, GSH assay, lipid peroxidation assay, iron assay","Human","HCT116 and CX-1 cells","PRKAA1 :+: (BECN1/SLC7A11), (BECN1/SLC7A11) :+: Ferroptosis","Validated","5'-AMP-activated protein kinase catalytic subunit alpha-1","Q13131","AAPK1_HUMAN",30057310]
["ELAVL1","ELAV like RNA binding protein 1","HGNC:3312","ELAVL1 siRNA led to ferroptosis resistance, whereas ELAVL1 plasmid contributed to classical ferroptotic events.","Immunohistochemistry, hydroxyproline assay, immunofluorescence, cell viability assay, western blot, qPCR, iron assay, ROS assay, RNAi","Human, mice","Human liver specimens and hepatic stellate cell; C57BL/6 mice; mouse hepatocyte and hepatic stellate cell; HSC-LX2 and HSC-T6 cells","ELAVL1 :+: Ferroptosis","Validated","ELAV-like protein 1","Q15717","ELAV1_HUMAN",30081711]
["BAP1","BRCA1 associated protein 1","HGNC:950","Suppresses SLC7A11-mediated cystine uptake and promotes ferroptosis. BAP1 mutants lose their abilities to repress SLC7A11 and to promote ferroptosis.","Flow cytometry, cell viability assay, cell death assay, western blot, gene knockout, lipid peroxidation assay, cystine uptake assay, GSH assay, RT-PCR, ChIP-qPCR, luciferase reporter assay, immunoprecipitation, IHC, ChIP-seq","Human","HEK-293T, Caki1, 786-O, 769-P, ACHN and NCI-H226 cell lines; RCC4,  UMRC2, SLR20, and UMRC6 cell lines; TK10 cell line; athymic nude mice","BAP1 :-: SLC7A11, SLC7A11 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Ubiquitin carboxyl-terminal hydrolase BAP1","Q92560","BAP1_HUMAN",30202049]
["TP53","Tumor protein p53","HGNC:11998","Facilitates ART-induced ferroptosis. Conversely, knockdown of P53 blocked ART-induced ferroptosis.","Western blot, immunofluorescence, siRNA","Rat","Hepatic stellate cell HSC‐T6","TP53 :+: Ferroptosis","Validated","Cellular tumor antigen p53","P04637","P53_HUMAN",30321484]
["ABCC1","ATP binding cassette subfamily C member 1","HGNC:51","Accelerates ferroptosis. Disruption of MRP1 inhibited ferroptosis potently.","Western blot, GSH assay, dead cell counts, gene transfection, CRISPR/Cas9, cell viability assay, RT-qPCR","Human","HAP1, H1299, U-2 OS cells","ABCC1 :-: GSH, GSH :-: Ferroptosis","Validated","Multidrug resistance-associated protein 1","P33527","MRP1_HUMAN",30726737]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Inhibition suppresses ferroptosis.","siRNA, western blot, microscopy imaging, fluorescence microscopy, real-time PCR","Human, mice","Male C57BL/6 mice, Caco-2 cells","ACSL4 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Long-chain-fatty-acid--CoA ligase 4","O60488","ACSL4_HUMAN",30737476]
["MIR6852","microRNA 6852","HGNC:49993","Promotes ferroptosis. Binds to LINC0033 and serves as a negative upstream regulator of CBS-mediated ferroptosis inhibition.","qRT-PCR, gene knockdown, luciferase assay, gene overexpression, MTT assay, colony-formation assay","Human","A549 and SPC-A-1 cells","MIR6852 :-: CBS, CBS :-: Ferroptosis","Validated","_NA_","_NA_",null,30787392]
["ACVR1B","Activin A receptor type 1B","HGNC:172","Inhibition attenuated erastin-induced ferroptosis.","Western blot, siRNA, glutathione assay, RT-PCR, cell viability assay","Human","HK-2 cells","ACVR1B :-: NFE2L2, NFE2L2 :-: Ferroptosis ","Validated","Activin receptor type-1B","P36896","ACV1B_HUMAN",30804470]
["TGFBR1","Transforming growth factor beta receptor 1","HGNC:11772","Inhibition attenuated erastin-induced ferroptosis.","Western blot, siRNA, glutathione assay, RT-PCR, cell viability assay","Human","HK-2 cells","TGFBR1 :-: NFE2L2, NFE2L2 :-: Ferroptosis","Validated","TGF-beta receptor type-1","P36897","TGFR1_HUMAN",30804470]
["BAP1","BRCA1 associated protein 1","HGNC:950","Promotes ferroptosis induced by class I ferroptosis inducer.","Immunoprecipitation, western blotting, cell death, cell viability, RT-PCR","Human","HEK293T cells","BAP1 :-: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","Ubiquitin carboxyl-terminal hydrolase BAP1","Q92560","BAP1_HUMAN",30907299]
["EPAS1","Endothelial PAS domain protein 1","HGNC:3374","A driver of ferroptosis susceptibility. Ablation reduced susceptibility to ferroptosis.","CRISPR, sgRNA, shRNA, immunoblot, cell viability, western blot","Mice","786-O cells","EPAS1 :+: HILPDA, EPAS1 :+: PLIN2, HILPDA :+: PUFA, PLIN2 :+: PUFA, PUFA :+: PUFA-phospholipid hydroperoxides, PUFA-phospholipid hydroperoxides :+: Ferroptosis","Validated","Endothelial PAS domain-containing protein 1","Q99814","EPAS1_HUMAN",30962421]
["HILPDA","Hypoxia inducible lipid droplet associated","HGNC:28859","Promotes ferroptosis sensitivity downstream of HIF-2alpha.","RNA-Seq, western blot, qRT-PCR, shRNA, cell viability, immunoblot ","Mice","786-O cells","HILPDA :+: PUFA, PUFA :+: PUFA-phospholipid hydroperoxides, PUFA-phospholipid hydroperoxides :+: Ferroptosis","Validated","Hypoxia-inducible lipid droplet-associated protein","Q9Y5L2","HLPDA_HUMAN",30962421]
["HIF1A","Hypoxia inducible factor 1 subunit alpha","HGNC:4910","Re-sensitized HIF-2alpha-null cells to ferroptosis.  Induce ferroptosis sensitivity in cancer cells.","cDNA screenin, cell viability","Mice","786-O cells","HIF1A :+: HILPDA, HIF1A :+: PLIN2, HILPDA :+: PUFA, PLIN2 :+: PUFA, PUFA :+: PUFA-phospholipid hydroperoxides, PUFA-phospholipid hydroperoxides :+: Ferroptosis","Validated","Hypoxia-inducible factor 1-alpha","Q16665","HIF1A_HUMAN",30962421]
["ALOX12","Arachidonate 12-lipoxygenase, 12S type","HGNC:429","An essential factor of p53-dependent ferroptosis. Loss of one Alox12 allele is sufficient to abrogate p53-mediated ferroptosis.","RNAi, qRT-PCR, western blot, CRISPR, cell death assay, GSH measurement, flow cytometer","Human, mice","H1299 cells, U2OS cells, mouse embryonic fibroblasts, mice","ALOX12 :+: PUFAs-OOH, PUFAs-OOH :+: Ferroptosis","Validated","Arachidonate 12-lipoxygenase","P18054","LOX12_HUMAN",30962574]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Required for ferroptosis induced by erastin. ACSL4-null cells are resistant to ferroptosis induced by either erastin.","Western blot, cell death assay, CRISPR","Human","U2OS","ACSL4 :+: Ferroptosis","Validated","Long-chain-fatty-acid--CoA ligase 4","O60488","ACSL4_HUMAN",30962574]
["HMOX1","Heme oxygenase 1","HGNC:5013","Enhances the ferroptotic process in PRDX6-silenced cells by promoting cellular accumulation of ferrous ions. Overexpression increases both erastin and RSL-3-induced lipid ROS.","Gene transfection, western blot, qRT-PCR, LOOH quantification","Human","H1299 cells","HMOX1 :+: Ferroptosis","Validated","Heme oxygenase 1","P09601","HMOX1_HUMAN",31036877]
["IFNG","Interferon gamma","HGNC:5438","Interferon gamma released from CD8+ T cells downregulates the expression of SLC3A2 and SLC7A11, and as a consequence, promotes tumour cell lipid peroxidation and ferroptosis.","BODIPY-C11, liperfluo staining, cell death measurement, FACS, cell proliferation and viability assay, qPCR, immunoblotting, glutathione quantification, MDA assay, glutamate assay","Human, mice","HT-1080, A375, B16-F0, ID8 cells, female NSG and C57BL/6 mice","IFNG :-: SLC3A2, IFNG :-: SLC7A11, SLC3A2 :+: System Xc-, SLC7A11 :+: System Xc-, System Xc- :+: Cystine, Cystine :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Interferon gamma","P01579","IFNG_HUMAN",31043744]
["ANO6","Anoctamin 6","HGNC:25240","Essential for ferroptosis. Inhibition blocked ferroptotic cell death induced by RSL3/erastin.","Immunocytochemistry, RT-PCR, TUNEL Assay, flow Cytometry, LDH assay, gene knockout, siRNA","Human, mice","A549, Cal27, HT29 and MG-63 cells, Cx3cr1-Cre mice","ANO6 :+: Ferroptosis","Validated","Anoctamin-6","Q4KMQ2","ANO6_HUMAN",31060306]
["LPIN1","Lipin 1","HGNC:13345","Overexpression of adipose lipin‐1 in mice facilitated the onset of hepatic ferroptosis.","Enzyme‐linked immunosorbent assay, lipid peroxidation assay, iron assay, MDA assay, qPCR, GSH assay","Mice","C57BL/6 mice","LPIN1 :+: Ferroptosis","Validated","Phosphatidate phosphatase LPIN1","Q14693","LPIN1_HUMAN",31061954]
["HMGB1","High mobility group box 1","HGNC:4983","Required for erastin-induced ferroptosis. Knockdown of HMGB1 decreased erastin-induced cell death.","Gene transfection, RNAi, cell viability assay, ELISA, LDH assay, flow cytometry, qPCR, western blot, electron microscopy","Human, mice","HL-60 cell, male NOD/SCID mice","HMGB1 :+: Ferroptosis","Validated","High mobility group protein B1","P09429","HMGB1_HUMAN",31105999]
["TNFAIP3","TNF alpha induced protein 3","HGNC:11896","Overexpression increased ROS generation and enhanced erastin-induced ferroptosis, whereas knockdown inhibited erastin-induced ferroptosis.","Lentivirus transduction, CCK-8 assay, DYE670 staining assay, ROS staining assay, RT-qPCR, western blot","Human","HUVEC cells","TNFAIP3 :+: ACSL4, ACSL4 :+: Lipid ROS, TNFAIP3 :-: GPX4, GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Tumor necrosis factor alpha-induced protein 3","P21580","TNAP3_HUMAN",31160087]
["TLR4","Toll like receptor 4","HGNC:11850","Knockdown inhibited ferroptosis.","Immunohistochemistry, western blot, cell death assay, siRNA","Rat","Sprague Dawley rats","TLR4 :+: Ferroptosis","Validated","Toll-like receptor 4","O00206","TLR4_HUMAN",31196626]
["NOX4","NADPH oxidase 4","HGNC:7891","Knockdown inhibited ferroptosis.","Immunohistochemistry, western blot, cell death assay, siRNA","Rat","Sprague Dawley rats","NOX4 :+: Ferroptosis","Validated","NADPH oxidase 4","Q9NPH5","NOX4_HUMAN",31196626]
["ATF3","Activating transcription factor 3","HGNC:785","Promotes ferroptosis induced by erastin.","Cell viability assay, transfection, lentiviral infection, qRT-PCR, western blot","Human","HT1080 cells, retinal pigment epithelial cells","ATF3 :-: SLC7A11, SLC7A11 :+: System Xc-, System Xc- :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Cyclic AMP-dependent transcription factor ATF-3","P18847","ATF3_HUMAN",31273299]
["ATM","ATM serine/threonine kinase","HGNC:795","Essential for ferroptosis. Genetic knockdown and chemical inhibition of ATM both suppress ferroptotic cell death.","siRNA, western blot, nuclear/cytosol fraction, cell viability, cell death, GSH assay, kinome screen, qRT-PCR, immunofluorescence assay","Human","MDA-MB-231, RCC4, 293T, and HT-1080 cell lines","ATM :-: MTF1, MTF1 :+: FTL, MTF1 :+: FTH1, FTL :-: Ferroptosis, FTH1 :-: Ferroptosis","Validated","Serine-protein kinase ATM","Q13315","ATM_HUMAN",31320750]
["YY1AP1","YY1 associated protein 1","HGNC:30935","Makes cells more sensitive to ferroptosis. Cells lacking YAP were no longer sensitised to ferroptosis.","Cell death, cell viability, lipid peroxidation, immunoblotting, CRISPR/Cas9, ChIP assay, qRT-PCR","Human, mice","Mouse embryonic fibroblasts, NF639 cells, human epithelial tumour cells, human mesothelioma cells, xenograft mouse","YAP :+: ACSL4, ACSL4 :+: Ferroptosis, YAP :+: TFRC,  TFRC :+: Ferroptosis","Validated","YY1-associated protein 1","Q9H869","YYAP1_HUMAN",31341276]
["EGLN2","Egl-9 family hypoxia inducible factor 2","HGNC:14660","Inhibiting EGLN2 activation diminished ferroptotic tumor cell death.","Cytotoxicity assays, western blot, immunoprecipitation, RNA interference, gene transfection, qPCR, MDA assay","Human, mice","Calu-1, THP1, HT1080, and HL-60 cells, athymic nude mice","EGLN2 :-: HIF1A, HIF1A  :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Egl nine homolog 2","Q96KS0","EGLN2_HUMAN",31355331]
["MIOX","Myo-inositol oxygenase","HGNC:14522","Overexpression exacerbates cell death, knockdown inhibits ferroptosis.","MTT assay, measurement of GPX4 activity, labile iron pool assay, evaluation of cell death and cell morphology, immunohistochemical staining","Human, mice","HK-2 cells, C57BL/6J mice","MIOX :-: GPX4, GPX4 :-: Ferroptosis, MIOX :-: GSH, GSH :-: Ferroptosis","Validated","Inositol oxygenase","Q9UGB7","MIOX_HUMAN",31437128]
["TAZ","Tafazzin","HGNC:11577","TAZ removal confers ferroptosis resistance, whereas overexpression of TAZS89A sensitizes cells to ferroptosis.","Cell viability, cell death, western blot, siRNA, qRT-PCR, microarray, immunofluorescence staining, ChIP-seq, Lipid ROS assay","Human, mice"," RCC4, 786O cells, JAX NOD.CB17-PrkdcSCID-J mice","TAZ :+: EMP1, EMP1 :+: NOX4, NOX4 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Tafazzin","Q16635","TAZ_HUMAN",31484063]
["MTDH","Metadherin","HGNC:29608","Can enhance sensitivity to inducers of ferroptosis. Enhances the vulnerability of cancer cells to ferroptosis.","Cell viability, qRT-PCR, western blotting, GSH assay, GPx4 activity assay, tumor xenograft model","Human, mice","Cell lines A549, H1975, DMS53, DMS273, KLE, AN3CA, RL95, Hec1A, Ishikawa, MDA-MB-231, MCF-7, Hec50, immunodeficient mice","MTDH :-: Cysteine, Cystein :-: Ferroptosis, MTDH :-: SLC3A2, SLC3A2 :-: Ferroptosis, MTDH :-: GPX4, GPX4 :-: Ferroptosis, MTDH :-: GSH, GSH :-: Ferroptosis, MTDH :+: Glutamate, Glutamate :+: Ferroptosis","Validated","Protein LYRIC","Q86UE4","LYRIC_HUMAN",31527591]
["IDH1","Isocitrate dehydrogenase (NADP(+)) 1","HGNC:5382","Deletion of the mutant IDH1 allele or pharmacological inhibition of mutant IDH1 confers resistance to erastin-induced ferroptosis. Ectopic expression of mutant IDH1 promotes ferroptosis.","Cell transfection, cell viability, GSH assay, lipid ROS assay","Human","HEK293T, HT-1080 and KYSE-170 cells","IDH1 :-: GPX4, GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis, IDH1 :-: GSH, GSH :-: Ferroptosis","Validated","Isocitrate dehydrogenase [NADP] cytoplasmic","O75874","IDHC_HUMAN",31591388]
["SIRT1","Sirtuin 1","HGNC:14929","Knockout partially mitigates ferroptosis.","Lipid peroxidation, iron and GSH assay, qRT-PCR, western blot","Mice","Mice in C57BL/6 background","SIRT1 :+: Ferroptosis","Predicted","NAD-dependent protein deacetylase sirtuin-1","Q96EB6","SIR1_HUMAN",31610175]
["TAZ","Tafazzin","HGNC:11577","TAZ removal confers ferroptosis resistance, while TAZS89A overexpression sensitizes cells to ferroptosis.","siRNA, qRT-PCR, western blot, cell viability, lipid ROS assay","Human","TOV-21G, CAOV2, CAOV2R, MDA-MB-231 cells","TAZ :+: ANGPTL4, ANGPTL4 :+: NOX2, NOX2 :+: Ferroptosis","Validated","Tafazzin","Q16635","TAZ_HUMAN",31641008]
["BECN1","Beclin 1","HGNC:1034","Overexpression aggravated isoflurane-induced cell damage by upregulating ferroptosis. This phenomenon was significantly attenuated by silencing of Beclin1.","Cell viability, RNAi, RT-qPCR, western blot, lipid peroxidation, iron, GSH and glutamate release assays","Human","SH-SY5Y cells","(Beclin1/SLC7A11) :-: System Xc-, System Xc- :-: Ferroptosis","Validated","Beclin-1","Q14457","BECN1_HUMAN",31650158]
["FBXW7","F-box and WD repeat domain containing 7","HGNC:16712","FBXW7 plasmid induces ferroptosis.","Cell viability, lipid peroxidation, iron, GSH and ROS assays, western blot, RT-PCR, RNA-seq","Human, mice","Hepatic stellate cells, C57BL/6 mice","FBXW7 :+: Ferroptosis","Deduced","F-box/WD repeat-containing protein 7","Q969H0","FBXW7_HUMAN",31679460]
["PANX1","Pannexin 1","HGNC:8599","Deletion protects against ferroptotic cell death. Silenced Panx1 expression significantly attenuated ferroptotic lipid peroxidation and iron accumulation induced by the ferroptosis inducer erastin.","Cell viability, cell death, qRT-PCR, western blot, lipid peroxidation detection, shRNA","Human, mice","HK-2 cells, C57BL/6 mice","PANX1 :-: HMOX1, HMOX1 :-: Ferroptosis, PANX1 :+: MAPK/ERK, MAPK/ERK :+: Ferroptinophagy, Ferroptinophagy :+: Ferroptosis","Validated","Pannexin-1","Q96RD7","PANX1_HUMAN",31694915]
["DNAJB6","DnaJ heat shock protein family (Hsp40) member B6","HGNC:14888","Promotes ferroptosis in esophageal squamous cell carcinoma.","Western blot, immunohistochemistry,lentivirus infection, colony-forming, RT?qPCR, CCK-8, transwell and mouse xenograft assays","Human, mice","Cell lines TE-1, Eca9706, Eca109, KYSE150, and KYSE450, BALB/c athymic nude mice","DNAJB6 :-: GPX4, GPX4 :-: Ferroptosis","Predicted","DnaJ homolog subfamily B member 6","O75190","DNJB6_HUMAN",31701262]
["BACH1","BTB domain and CNC homolog 1","HGNC:935","Promotes ferroptosis by repressing the transcription of a subset of the erastin-induced protective genes.","Western blotting, qPCR, cell death, labile iron detection, RNA-seq","Mice","C57BL/6J mice, mouse embryonic fibroblasts","BACH1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Transcription regulator protein BACH1","O14867","BACH1_HUMAN",31740582]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Overexpression induced ferroptosis. The opposite results were observed when ACSL4 was silenced.","Western blotting, immunofluorescence, 5?HETE, LDH and CCK-8 cell viability assay","Human","HEB, T98G, U87 MG and U251 cells","ACSL4 :-: GPX4, GPX4 :-: Ferroptosis","Validated","Long-chain-fatty-acid--CoA ligase 4","O60488","ACSL4_HUMAN",31789401]
["LONP1","Lon peptidase 1, mitochondrial","HGNC:9479","Inhibition of LONP1 negatively regulates erastin-induced cell death.","Cell viability, western blot, qRT-PCR, lipid peroxidation assay, glutathione assay","Human","PANC1, BxPC3 cells","LONP1 :-: Nrf2/Keap1 pathway, Nrf2/Keap1 pathway :-: Ferroptosis, LONP1 :-: GPX4, GPX4 :-: Ferroptosis","Validated","Lon protease homolog, mitochondrial","P36776","LONM_HUMAN",31822343]
["CD82","CD82 molecule","HGNC:6210","This study identified that the high expression of the KAI1 gene promoted the occurrence of ferroptosis in PC cells through its extensive effect on FPN and GPX4.","western blotting, cck-8, flow cytometry","Human","PC cell line MIA PaCa?2","KAI1 :+: Ferroptosis","Validated",{"$nulls":3},33655331]
["IL1B","interleukin 1 beta","HGNC:5992","We observed that both IL-1β and FAC induced reactive oxygen species (ROS), and lipid ROS accumulation and ferroptosis related protein expression changes in chondrocytes. Ferrostatin-1, a ferroptosis specific inhibitor, attenuated the cytotoxicity, ROS and lipid-ROS accumulation and ferroptosis related protein expression changes induced by IL-1β and FAC and facilitated the activation of Nrf2 antioxidant system.","_NA_","Human, mice","chondrocytes, DMM mouse model","IL1B :+: ROS, ROS :+: Ferroptosis","Validated","_NA_","_NA_",null,33376672]
["CTSB","cathepsin B","HGNC:2527","Cathepsin B is an executioner of ferroptosis","_NA_","_NA_","_NA_","CTSB :+: Ferroptosis","Validated","_NA_","_NA_",null,33340545]
["POR","cytochrome p450 oxidoreductase","HGNC:9208","The oxidoreductases POR and CYB5R1 are required for ferroptosis. Knockdown of POR in vivo protects ConA-induced ferroptotic liver damage","CRISPR-Cas9, immunoblot analysis, cell viability, LC-MS/MS","Human, mice","HeLa, HT-1080, and mouse embryonic fibroblast (MEF) cells","POR :+: hydrogen peroxide, hydrogen peroxide :+: PUFA peroxidation, PUFA peroxidation :+: Ferroptosis","Validated","_NA_","_NA_",null,33321093]
["CYB5R1","cytochrome b5 reductase 1","HGNC:13397","The oxidoreductases POR and CYB5R1 are required for ferroptosis. Genetic knockout of POR and CYB5R1 decreases cellular hydrogen peroxide generation, preventing lipid peroxidation and ferroptosis.","shRNA, gene expression","Human","HeLa cells","CYB5R1 :+: hydrogen peroxide, hydrogen peroxide :+: PUFA peroxidation, PUFA peroxidation :+: Ferroptosis","Validated","_NA_","_NA_",null,33321093]
["ELOVL5","ELOVL fatty acid elongase 5","HGNC:21308","Biochemical and lipidomics analyses support the hypothesis that ELOVL5 and FADS1 are required to maintain intracellular levels of AA and AdA and promote ferroptosis.","Cysteine/Methionine Deprivation, Western Blotting, LC-MS/MS","Human","YCC-16 cells","ELOVL5 :+: Arachidonic acid, Arachidonic acid :+: Ferroptosis","Validated","_NA_","_NA_",null,33288688]
["FADS1","fatty acid desaturase 1","HGNC:3574","Biochemical and lipidomics analyses support the hypothesis that ELOVL5 and FADS1 are required to maintain intracellular levels of AA and AdA and promote ferroptosis.","Cysteine/Methionine Deprivation, Western Blotting, LC-MS/MS","Human","YCC-16 cells","FADS1 :+: Arachidonic acid, Arachidonic acid :+: Ferroptosis","Validated","_NA_","_NA_",null,33288688]
["ALOX12","arachidonate 12-lipoxygenase, 12S type","HGNC:429","Hydrogen sulfide guards myoblasts from ferroptosis by inhibiting ALOX12 acetylation.","Cell viability assay, Measurement of oxidative stress, lipid peroxidation and acetyl-CoA content, Immunoblotting","Mice","myoblasts and skeletal muscles","ALOX12 :+: Lipid peroxide, Lipid peroxide :+: Ferroptosis","Deduced","_NA_","_NA_",null,33290842]
["FBW7","F-box and WD repeat domain containing 7","HGNC:16712","Results revealed that FBW7 regulated lipid peroxidation and promoted ferroptosis, a non-apoptotic form of cell death.","Cell viability and cell death, siRNA, Western blot, qPCR, ChIP, MDA and GSH/GSSG assay","Human","cell lines PANC-1 and SW1990","FBW7 :-: (NR4A1/SCD1), (NR4A1/SCD1) :+: SCD1, SCD1 :-: Ferroptosis","Validated","_NA_","_NA_",null,33271455]
["PTEN","phosphatase and tensin homolog","HGNC:9588","Notably, we found that cancer cells carrying PIK3CA activating mutation or PTEN deletion appeared to be more resistant to RSL3. These mutations lead to the activation of the oncogenic PI3K-AKT signaling pathway. Therefore, sustained activation of the PI3K-AKT-mTOR pathway protects cancer cells from ferroptotic cell death.","Cell death","Human","BT474, MDA-MB-453","PTEN :-: PI3K-AKT pathway, PI3K-AKT pathway :-: Ferroptosis","Deduced","_NA_","_NA_",null,33229547]
["NR1D1","nuclear receptor subfamily 1 group D member 1","HGNC:7962","Targeted inhibition of Rev-erb-α/β limits ferroptosis to ameliorate folic acid-induced AKI in mice.","_NA_","Mice","_NA_","(NR1D1/RORE) :-: SLC7A11, SLC7A11 :-: Ferroptosis, (NR1D1/RORE) :-: HO1, HO1 :-: Ferroptosis","Deduced","_NA_","_NA_",null,33068011]
["NR1D2","nuclear receptor subfamily 1 group D member 2","HGNC:7963","Targeted inhibition of Rev-erb-α/β limits ferroptosis to ameliorate folic acid-induced AKI in mice.","_NA_","Mice","_NA_","(NR1D2/RORE) :-: SLC7A11, SLC7A11 :-: Ferroptosis, (NR1D2/RORE) :-: HO1, HO1 :-: Ferroptosis","Deduced","_NA_","_NA_",null,33068011]
["TBK1","TANK binding kinase 1","HGNC:11584","hTBK1-c.978T>A mutation significantly inhibited the proliferation of NSC-34 cells via inducing cell ferroptosis, while the effect of TBK1 mutation was notably reversed by Ferrostatin-1 or p62 siRNA.","_NA_","Mice","_NA_","TBK1-c.978T>A :+: KEAP1, KEAP1 :+: Ferroptosis","Validated","_NA_","_NA_",null,33312375]
["IL6","interleukin 6","HGNC:6018","IL-6 exposure caused cartilage cell ferroptosis by inducing cellular oxidative stress and disturbing iron homeostasis. Overexpressing miR-10a-5p suppressed IL-6R expression, and partially abolished IL-6-induced ferroptosis.","_NA_","Human, mice","_NA_","IL-6 :-: miR-10a-5p, miR-10a-5p :-: IL-6R, IL-6R :+: Ferroptosis","Validated","_NA_","_NA_",null,33166496]
["USP7","ubiquitin specific peptidase 7","HGNC:12630","USP7 promotes ferroptosis in the I/R-treated hearts via activation of p53/TfR1 pathway.","_NA_","Rat","_NA_","USP7 :+: p53/TfR1, p53/TfR1 :+: Ferroptosis","Validated","_NA_","_NA_",null,33157209]
["miR-182-5p","_NA_","_NA_","In conclusion, we demonstrated that I/R induced upregulation of miR-182-5p and miR-378a-3p, leading to activation of ferroptosis in renal injury through downregulation of GPX4 and SLC7A11.","_NA_","Human, rat","_NA_","miR-182-5p :-: GPX4, GPX4 :-: Ferroptosis","Validated","_NA_","_NA_",null,33116120]
["miR-378a-3p","_NA_","_NA_","In conclusion, we demonstrated that I/R induced upregulation of miR-182-5p and miR-378a-3p, leading to activation of ferroptosis in renal injury through downregulation of GPX4 and SLC7A11.","_NA_","Human, rat","_NA_","miR-378a-3p :-: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","_NA_","_NA_",null,33116120]
["CTSB","cathepsin B","HGNC:2527","Mechanistically, nuclear CTSB accumulation causes DNA damage and subsequent activation of the stimulator of interferon response CGAMP interactor 1 (STING1/STING)-dependent DNA sensor pathway, which ultimately leads to autophagy-dependent ferroptosis.","_NA_","Human, mice","_NA_","CTSB :+: DNA damage, DNA damage :+: DNA sensor pathway, DNA sensor pathway :+: Ferroptosis","Deduced","_NA_","_NA_",null,33268027]
["ACSL4","acyl-CoA synthetase long chain family member 4","HGNC:3571","Inhibition of ACSL4 attenuates ferroptotic damage after pulmonary ischemia-reperfusion.","_NA_","Human, mice","_NA_","ACSL4 :-: GPX4, GPX4 :-: Ferroptosis","Deduced","_NA_","_NA_",null,33070393]
["ATF4","activating transcription factor 4","HGNC:786","We identify ATF4 as differentially expressed in P47 and S47 cells and show that ATF4 can reverse the redox status and rescue metabolism of S47 cells, as well as increase sensitivity to ferroptosis.","_NA_","Human, mice","_NA_","ATF4 :+: Ferroptosis","Validated","_NA_","_NA_",null,33055209]
["BECN1","Beclin 1","HGNC:1034","Beclin1 haploinsufficiency rescues low ambient temperature-induced cardiac remodeling and contractile dysfunction through inhibition of ferroptosis and mitochondrial injury.","_NA_","Mice","_NA_","BECN1 :+: Ferroptosis","Deduced","_NA_","_NA_",null,33058849]
["AQP3","aquaporin 3 (Gill blood group)","HGNC:636","Further, we observed that aquaporin (AQP) 3, 5, and 8 bind nicotinamide-adenine dinucleotide phosphate oxidase 2 and regulate the permeability of extracellular H2O2, thereby contributing to ferroptosis.","_NA_","_NA_","_NA_","(AQP3/NOX2) :+: H2O2, H2O2 :+: Ferroptosis","Deduced","_NA_","_NA_",null,33017631]
["AQP5","aquaporin 5","HGNC:638","Further, we observed that aquaporin (AQP) 3, 5, and 8 bind nicotinamide-adenine dinucleotide phosphate oxidase 2 and regulate the permeability of extracellular H2O2, thereby contributing to ferroptosis.","_NA_","_NA_","_NA_","(AQP5/NOX2) :+: H2O2, H2O2 :+: Ferroptosis","Deduced","_NA_","_NA_",null,33017631]
["AQP8","aquaporin 8","HGNC:642","Further, we observed that aquaporin (AQP) 3, 5, and 8 bind nicotinamide-adenine dinucleotide phosphate oxidase 2 and regulate the permeability of extracellular H2O2, thereby contributing to ferroptosis.","_NA_","_NA_","_NA_","(AQP8/NOX2) :+: H2O2, H2O2 :+: Ferroptosis","Deduced","_NA_","_NA_",null,33017631]
["LINC00618","long intergenic non-protein coding RNA 618","HGNC:20110","LINC00618 also accelerates ferroptosis by increasing the levels of lipid reactive oxygen species (ROS) and iron, two surrogate markers of ferroptosis, and decreasing the expression of solute carrier family 7 member 11 (SLC7A11)","_NA_","Human, mice","_NA_","LINC00618 :+: Lipid ROS, Lipid ROS :+: Ferroptosis,  LINC00618 :+: Iron, Iron :+: Ferroptosis, LINC00618 :-: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","_NA_","_NA_",null,33002417]
["IREB2","iron responsive element binding protein 2","HGNC:6115"," Further studies have found that ART could lead to the accumulation of IRP 2 a in hepatic stellate cell by inhibiting the ubiquitination of it, thus inducing the increase of iron in HSC (hepatic stellate cell), which could product a large number of ROS (reactive oxide species), resulting the occurrence of ferroptosis in cells.","_NA_","Human","_NA_","IRP2 :+: Fe, Fe :+: ROS, ROS :+: Ferroptosis","Deduced","_NA_","_NA_",null,32947011]
["MT1DP","metallothionein 1D, pseudogene","HGNC:7396","We found that ectopic expression of MT1DP sensitized A549 and H1299 cells to erastin-induced ferroptosis through downregulation of NRF2.","_NA_","Human","_NA_","MT1DP :+: miR-365a-3p, miR-365a-3p :-: NRF2, NRF2 :-: Ferroptosis","Validated","_NA_","_NA_",null,32929075]
["ACSL4","acyl-CoA synthetase long chain family member 4","HGNC:3571","_NA_","_NA_","Human","_NA_","ACSL4 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["PEX10","peroxisomal biogenesis factor 10","HGNC:8851","Genome-wide CRISPR screens identify peroxisome components as contributors to ferroptosis susceptibility.","_NA_","Human, mice","_NA_","PEX10 :+: PUFA-ePLs, PUFA-ePLs :+: Ferroptosis","Validated","_NA_","_NA_",null,32939090]
["KEAP1","kelch like ECH associated protein 1","HGNC:23177","_NA_","_NA_","Human","_NA_","KEAP1 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["AGPAT3","1-acylglycerol-3-phosphate O-acyltransferase 3","HGNC:326","_NA_","_NA_","Human","_NA_","AGPAT3 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["PEX12","peroxisomal biogenesis factor 12","HGNC:8854","Genome-wide CRISPR screens identify peroxisome components as contributors to ferroptosis susceptibility.","_NA_","Human","_NA_","PEX12 :+: PUFA-ePLs, PUFA-ePLs :+: Ferroptosis","Validated","_NA_","_NA_",null,32939090]
["CHP1","calcineurin like EF-hand protein 1","HGNC:17433","_NA_","_NA_","Human","_NA_","CHP1 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["GPAT4","glycerol-3-phosphate acyltransferase 4","HGNC:20880","_NA_","_NA_","Human","_NA_","AGPAT6 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["BRPF1","bromodomain and PHD finger containing 1","HGNC:14255","_NA_","_NA_","Human","_NA_","BRPF1 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["OSBPL9","oxysterol binding protein like 9","HGNC:16386","_NA_","_NA_","Human","_NA_","OSBPL9 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["INTS2","integrator complex subunit 2","HGNC:29241","_NA_","_NA_","Human","_NA_","INTS2 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["MMD","monocyte to macrophage differentiation associated","HGNC:7153","_NA_","_NA_","Human","_NA_","MMD :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["CYP4F8","cytochrome P450 family 4 subfamily F member 8","HGNC:2648","_NA_","_NA_","Human","_NA_","CYP4F8 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["MLLT1","MLLT1 super elongation complex subunit","HGNC:7134","_NA_","_NA_","Human","_NA_","MLLT1 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["TTPA","alpha tocopherol transfer protein","HGNC:12404","_NA_","_NA_","Human","_NA_","TTPA :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["GRIA3","glutamate ionotropic receptor AMPA type subunit 3","HGNC:4573","_NA_","_NA_","Human","_NA_","GRIA3 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["EPT1","_NA_","_NA_","_NA_","_NA_","Human","_NA_","EPT1 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["POM121L12","POM121 transmembrane nucleoporin like 12","HGNC:25369","_NA_","_NA_","Human","_NA_","POM121L12 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["LIG3","DNA ligase 3","HGNC:6600","_NA_","_NA_","Human, mice","_NA_","LIG3 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["AEBP2","AE binding protein 2","HGNC:24051","_NA_","_NA_","Human","_NA_","AEBP2 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["AGPS","alkylglycerone phosphate synthase","HGNC:327","Genome-wide CRISPR screens identify peroxisome components as contributors to ferroptosis susceptibility.","_NA_","Human","_NA_","AGPS :+: PUFA-ePLs, PUFA-ePLs :+: Ferroptosis","Validated","_NA_","_NA_",null,32939090]
["CDCA3","cell division cycle associated 3","HGNC:14624","_NA_","_NA_","Human","_NA_","CDCA3 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["PEX2","peroxisomal biogenesis factor 2","HGNC:9717","Genome-wide CRISPR screens identify peroxisome components as contributors to ferroptosis susceptibility.","_NA_","Human","_NA_","PEX2 :+: PUFA-ePLs, PUFA-ePLs :+: Ferroptosis","Validated","_NA_","_NA_",null,32939090]
["LPCAT3","lysophosphatidylcholine acyltransferase 3","HGNC:30244","_NA_","_NA_","Human","_NA_","LPCAT3 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["PEX6","peroxisomal biogenesis factor 6","HGNC:8859","_NA_","_NA_","Human","_NA_","PEX6 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["TIMM9","translocase of inner mitochondrial membrane 9","HGNC:11819","_NA_","_NA_","Human","_NA_","TIMM9 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["DCAF7","DDB1 and CUL4 associated factor 7","HGNC:30915","_NA_","_NA_","Human","_NA_","DCAF7 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["LCE2C","late cornified envelope 2C","HGNC:29460","_NA_","_NA_","Human","_NA_","LCE2C :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["FAR1","fatty acyl-CoA reductase 1","HGNC:26222","Genome-wide CRISPR screens identify peroxisome components as contributors to ferroptosis susceptibility.","_NA_","Human","_NA_","FAR1 :+: PUFA-ePLs, PUFA-ePLs :+: Ferroptosis","Validated","_NA_","_NA_",null,32939090]
["PHF21A","PHD finger protein 21A","HGNC:24156","_NA_","_NA_","Human","_NA_","PHF21A :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["SMAD7","SMAD family member 7","HGNC:6773","_NA_","_NA_","Human","_NA_","SMAD7 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["LYRM1","LYR motif containing 1","HGNC:25074","_NA_","_NA_","Human","_NA_","LYRM1 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["AMN","amnion associated transmembrane protein","HGNC:14604","_NA_","_NA_","Human","_NA_","AMN :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["PEX3","peroxisomal biogenesis factor 3","HGNC:8858","Genome-wide CRISPR screens identify peroxisome components as contributors to ferroptosis susceptibility.","_NA_","Human","_NA_","PEX3 :+: PUFA-ePLs, PUFA-ePLs :+: Ferroptosis","Validated","_NA_","_NA_",null,32939090]
["MTCH1","mitochondrial carrier 1","HGNC:17586","_NA_","_NA_","Human","_NA_","MTCH1 :+: Ferroptosis","Screened","_NA_","_NA_",null,32939090]
["ZEB1","zinc finger E-box binding homeobox 1","HGNC:11642","CDH1 silencing or ZEB1 overexpression increased the susceptibility to ferroptosis, whereas CDH overexpression or ZEB1 silencing decreased the susceptibility, in vitro and in vivo.","_NA_","Human, mice","_NA_","ZEB1 :+: Ferroptosis","Validated","_NA_","_NA_",null,32896720]
["SIRT1","sirtuin 1","HGNC:14929","Histone deacetylase SIRT1 gene silencing or pharmacological inhibition by EX-527 suppressed EMT and consequently decreased ferroptosis, whereas SIRT inducers, resveratrol and SRT1720, increased ferroptosis.","_NA_","Human, mice","_NA_","SIRT1 :+: EMT, EMT :+: Ferroptosis","Validated","_NA_","_NA_",null,32896720]
["ACADSB","acyl-CoA dehydrogenase short/branched chain","HGNC:91","Overexpression of ACADSB inhibits CRC cell migration, invasion, and proliferation, while ACADSB knockdown has the opposite effect. More importantly, the study finds that ACADSB negatively regulates expression of glutathione reductase and glutathione peroxidase 4, the two main enzymes responsible for clearing glutathione (GSH) in CRC cells.","_NA_","Human","_NA_","ACADSB :-: GSR, GSR :-: Ferroptosis, ACADSB :-: GPX4, GPX4 :-: Ferroptosis, ACADSB :-: GSH, GSH :-: Ferroptosis","Deduced","_NA_","_NA_",null,32776663]
["PVT1","Pvt1 oncogene","HGNC:9709","PVT1 silencing or miR-214 overexpression significantly reduced infarct size and suppressed ferroptosis in vivo.","_NA_","Human, mice","_NA_","PVT1 :+: Ferroptosis","Validated","_NA_","_NA_",null,32827544]
["hsa_circ_0008367","_NA_","_NA_","These evidences suggested cIARS to be a positive regulator of ferroptosis in HCC cells.","_NA_","Human","_NA_","hsa_circ_0008367 :+: Ferroptosis","Validated","_NA_","_NA_",null,32802409]
["SLC39A14","solute carrier family 39 member 14","HGNC:20858","Loss of hepatic Slc39a14 protects against ferroptosis-induced liver fibrosis in Trf-LKO mice","_NA_","Mice","_NA_","SLC39A14 :+: Ferroptosis","Validated","_NA_","_NA_",null,32374849]
["NCOA4","nuclear receptor coactivator 4","HGNC:7671","Additionally, NCOA4 expression was inhibited, which reduced degradation of ferritin and suppressed ferroptosis.","_NA_","Human","_NA_","NCOA4 :-: FTMT :-: Ferroptosis","Deduced","Nuclear receptor coactivator 4","Q13772","NCOA4_HUMAN",32810738]
["MAP3K11","mitogen-activated protein kinase kinase kinase 11","HGNC:6850","Inhibition of MLK3 improves cardiac function and inhibits ferroptosis at week 8 of TAC.","_NA_","Mice","_NA_","MAP3K11 :+: Ferroptosis","Validated","Mitogen-activated protein kinase kinase kinase 11","Q16584","M3K11_HUMAN",32710001]
["GSK3B","glycogen synthase kinase 3 beta","HGNC:4617","Silence of GSK-3β blocked erastin-induced ferroptosis with less production of ROS and malondialdehyde (MDA) via upregulation of GPX4 and downregulation of arachidonate 15-lipoxygenase (Alox15), overexpression of GSK-3β enhanced erastin-triggered ferroptosis with elevated ROS and MDA","_NA_","Human","_NA_","GSK3B :+: Lipid ROS, Lipid ROS :+: Ferroptosis, GSK3B :-: GPX4, GPX4 :-: Ferroptosis","Validated","Glycogen synthase kinase-3 beta","P49841","GSK3B_HUMAN",32642794]