|  | SWISS-MODEL Homology Modelling Report

## Model Building Report

This document lists the results for the homology modelling project "13_July_SF_Bin2_scaffold_7244_c1_4398-5624_1" submitted to SWISS-MODEL workspace on March 29, 2023, 7:37 p.m..The submitted primary amino acid sequence is given in Table T1.

If you use any results in your research, please cite the relevant publications:

- Waterhouse, A., Bertoni, M., Bienert, S., Studer, G., Tauriello, G., Gumienny, R., Heer, F.T., de Beer, T.A.P., Rempfer, C., Bordoli, L., Lepore, R., Schwede, T. SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. 46(W1), W296-W303 (2018).
- Bienert, S., Waterhouse, A., de Beer, T.A.P., Tauriello, G., Studer, G., Bordoli, L., Schwede, T. The SWISS-MODEL Repository - new features and functionality. Nucleic Acids Res. 45, D313-D319 (2017).
- Studer, G., Tauriello, G., Bienert, S., Biasini, M., Johner, N., Schwede, T. ProMod3 - A versatile homology modelling toolbox. PLOS Comp. Biol. 17(1), e1008667 (2021).
- Studer, G., Rempfer, C., Waterhouse, A.M., Gumienny, G., Haas, J., Schwede, T. QMEANDisCo - distance constraints applied on model quality estimation. Bioinformatics 36, 1765-1771 (2020).
- Bertoni, M., Kiefer, F., Biasini, M., Bordoli, L., Schwede, T. Modeling protein quaternary structure of homo- and hetero-oligomers beyond binary interactions by homology. Scientific Reports 7 (2017).

## Results

The SWISS-MODEL template library (SMTL version 2023-03-23, PDB release 2023-03-17) was searched with for evolutionary related structures matching the target sequence in Table T1. For details on the template search, see Materials and Methods. Overall 444 templates were found (Table T2).

## Models

The following models were built (see Materials and Methods "Model Building"):

### Model #01 | File | Built with | Oligo-State | Ligands | GMQE | QMEANDisCo Global
--- | --- | --- | --- | --- | --- | ---
| PDB | ProMod3 3.2.1 | monomer | None | 0.68 | 0.66 ± 0.05

Template | Seq Identity | Oligo-state | QSQE | Found by | Method | Resolution | Seq Similarity | Range | Coverage | Description
--- | --- | --- | --- | --- | --- | --- | --- | --- | --- | ---
7b04.1.B | 45.15 | monomer | 0.00 | BLAST | X-ray | 2.97Å | 0.43 | 30 - 396 | 0.96 | Nitrite oxidoreductase subunit A

### Excluded ligands

Ligand Name.Number | Reason for Exclusion | Description
--- | --- | ---
CA.10 | Binding site not conserved. | CALCIUM ION
CA.11 | Binding site not conserved. | CALCIUM ION
F3S.4 | Binding site not conserved. | FE3-S4 CLUSTER
HEM.9 | Binding site not conserved. | PROTOPORPHYRIN IX CONTAINING FE
MD1.5 | Binding site not conserved. | PHOSPHORIC ACID 4-(2-AMINO-4-OXO-3,4,5,6,-TETRAHYDRO-PTERIDIN-6-YL)-2-HYDROXY-3,4-DIMERCAPTO-BUT-3-EN-YL ESTER GUANYLATE ESTER
MD1.6 | Binding site not conserved. | PHOSPHORIC ACID 4-(2-AMINO-4-OXO-3,4,5,6,-TETRAHYDRO-PTERIDIN-6-YL)-2-HYDROXY-3,4-DIMERCAPTO-BUT-3-EN-YL ESTER GUANYLATE ESTER
MO.7 | Binding site not conserved. | MOLYBDENUM ATOM
SF4.1 | Binding site not conserved. | IRON/SULFUR CLUSTER
SF4.2 | Binding site not conserved. | IRON/SULFUR CLUSTER
SF4.3 | Binding site not conserved. | IRON/SULFUR CLUSTER
SF4.8 | Binding site not conserved. | IRON/SULFUR CLUSTER

    Target    NNVNRREFLQWIGAAGFSTFALSASNAWGLQ------AIENPLAAYPNREWEKTYRDLWKSDASFTFLCAPNDTHNCILN
    7b04.1.B  --LTRRAFLQVAGATG-ATLTL-AKNAMAFRLLKPAVVVDNPLDTYPDRRWESVYRDQYQYDRTFTYCCSPNDTHACRIR

    Target    AHVRDGVITRIGPTMKYGEATDLYGSKVTHRWDPRVCQKGLALTRRFYGDRRVRYPMVRKGFKAWADKGFPREKDGRPPK
    7b04.1.B  AFVRNNVMMRVEQNYDHQNYSDLYGNKATRNWNPRMCLKGYTFHRRVYGPYRLRYPLIRKGWKRWADDGFPELTPENKTK

    Target    DYF-NRARDEWLRLTHEEAADLVAAALINIATTYSGDNGQKLLLQQGYEKEIVEATRGAGTQVLKFRGGMPLLGLTRIFG
    7b04.1.B  YMFDNRGNDELLRASWDEAFTYASKGIIHITKKYSGPEGAQKLIDQGYPKEMVDRMQGAGTRTFKGRGGMGLLGVIGKYG

    Target    LYRMANSMALLDHKIRGVKPEDALGARGWDNYSWHTDLPPGHPMVTGQQTVDFDLHAVEQARIVVVWGMNWVTTKMPDTH
    7b04.1.B  MYRFNNCLAIVDAHNRGVGPDQALGGRNWSNYTWHGDQAPGHPFSHGLQTSDVDMNDVRFSKLLIQTGKNLIENKMPEAH

    Target    WLTEARLKGTKVVVIACEYSSSSIKADDAIVVRPGTTPALALGLCNVIMREKIYDGDYVRRFSDLPLLVRADNLKLLRAE
    7b04.1.B  WVTEVMERGGKIVVITPEYSPSAQKADYWIPIRNNTDTALFLGITKILIDNKWYDADYVKKFTDFPLLIRTDTLKRVSPK

    Target    EVFGTPQAALKNQTR
    7b04.1.B  DII------------

### Model #03 | File | Built with | Oligo-State | Ligands | GMQE | QMEANDisCo Global
--- | --- | --- | --- | --- | --- | ---
| PDB | ProMod3 3.2.1 | monomer | None | 0.41 | 0.46 ± 0.05

Template | Seq Identity | Oligo-state | QSQE | Found by | Method | Resolution | Seq Similarity | Range | Coverage | Description
--- | --- | --- | --- | --- | --- | --- | --- | --- | --- | ---
1e5v.2.A | 24.29 | monomer | 0.00 | HHblits | X-ray | 2.40Å | 0.31 | 56 - 408 | 0.78 | Dimethyl sulfoxide/trimethylamine N-oxide reductase

### Excluded ligands

Ligand Name.Number | Reason for Exclusion | Description
--- | --- | ---
2MO.3 | Binding site not conserved. | MOLYBDENUM (IV)OXIDE
PGD.1 | Binding site not conserved. | 2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE GUANOSINE DINUCLEOTIDE
PGD.2 | Binding site not conserved. | 2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE GUANOSINE DINUCLEOTIDE
SO4.4 | Not biologically relevant. | SULFATE ION

    Target    NNVNRREFLQWIGAAGFSTFALSASNAWGLQAIENPLAAYPNREWEKTYRDLWKSDASFTFLCAPNDTHNCI-LNAHVRD
    1e5v.2.A  -ELYRRAFLSYSVAPGALGMFGR-SL---LAK-----GARA--EA----------LAN---GT-VMSGSHWGVFTATVEN

    Target    GVITRIGPTMKYGEATDLYGSKVTHRWDPRVCQKGLALTRRFYGDRRVRYPMVRKGFKAWADKGFPREKDGRPPKDYFNR
    1e5v.2.A  GRATAFTPWEK------------DP----HPSPMLAGVLDSIYSPTRIKYPMVRREFL---EKGVN--------ADRSTR

    Target    ARDEWLRLTHEEAADLVAAALINIATTYSGDNGQKLLLQQGYEKEIVEATRGAGTQVLKFRGGMPLLGLTRIFGLYRMAN
    1e5v.2.A  GNGDFVRVSWDQALDLVAAEVKRVEETYGPEG---VFGG-SYGWKSPGRLHNCTTLLRRM---------LTLAGGY--VN

    Target    SMALLDHKIRGVKPEDALGARGWDNYSWH-----TDLPPGHPMVTGQQTVDFDLHAVEQARIVVVWGMNWVTTKMPD---
    1e5v.2.A  GA---------------------GDYSTGAAQVIMPHVVGTLEVYEQQ--TAWPVLAENTEVMVFWAADPIKTSQIGWVI

    Target    -----THWLTEARLKGTKVVVIACEYSSSSIK-ADDAIVVRPGTTPALALGLCNVIMREKIYDGDYVRRFSDLPL----L
    1e5v.2.A  PEHGAYPGLEALKAKGTKVIVIDPVRTKTVEFFGAEHITPKPQTDVAIMLGMAHTLVAEDLYDKDFIANYTSGFDKFLPY

    Target    VR----ADNLKLLRAEEVFGTPQAALKNQTR
    1e5v.2.A  LDGETDSTPKTAEWAEGISGVPAETIKELAR

### Model #02 | File | Built with | Oligo-State | Ligands | GMQE | QMEANDisCo Global
--- | --- | --- | --- | --- | --- | ---
| PDB | ProMod3 3.2.1 | monomer | None | 0.39 | 0.45 ± 0.05

Template | Seq Identity | Oligo-state | QSQE | Found by | Method | Resolution | Seq Similarity | Range | Coverage | Description
--- | --- | --- | --- | --- | --- | --- | --- | --- | --- | ---
3ir5.1.A | 30.49 | monomer | 0.00 | BLAST | X-ray | 2.30Å | 0.37 | 42 - 382 | 0.75 | Respiratory nitrate reductase 1 alpha chain

### Excluded ligands

Ligand Name.Number | Reason for Exclusion | Description
--- | --- | ---
6MO.3 | Binding site not conserved. | MOLYBDENUM(VI) ION
AGA.5 | Binding site not conserved. | (1S)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PENTANOYLOXY)METHYL]ETHYL OCTANOATE
F3S.9 | Binding site not conserved. | FE3-S4 CLUSTER
HEM.10 | Binding site not conserved. | PROTOPORPHYRIN IX CONTAINING FE
HEM.11 | Binding site not conserved. | PROTOPORPHYRIN IX CONTAINING FE
MD1.1 | Binding site not conserved. | PHOSPHORIC ACID 4-(2-AMINO-4-OXO-3,4,5,6,-TETRAHYDRO-PTERIDIN-6-YL)-2-HYDROXY-3,4-DIMERCAPTO-BUT-3-EN-YL ESTER GUANYLATE ESTER
MD1.2 | Binding site not conserved. | PHOSPHORIC ACID 4-(2-AMINO-4-OXO-3,4,5,6,-TETRAHYDRO-PTERIDIN-6-YL)-2-HYDROXY-3,4-DIMERCAPTO-BUT-3-EN-YL ESTER GUANYLATE ESTER
SF4.4 | Binding site not conserved. | IRON/SULFUR CLUSTER
SF4.6 | Binding site not conserved. | IRON/SULFUR CLUSTER
SF4.7 | Binding site not conserved. | IRON/SULFUR CLUSTER
SF4.8 | Binding site not conserved. | IRON/SULFUR CLUSTER

    Target    NNVNRREFLQWIGAAGFSTFALSASNAWGLQAIENPLAAYPNREWEKTYRDLWKSDASFTFLCAPNDTHNCILNAHVRDG
    3ir5.1.A  -----------------------------------------NRDWEDGYRQRWQHDKIVRSTCGVNCTGSCSWKIYVKNG

    Target    VITRIGPTMKYGEA-TDLYGSKVTHRWDPRVCQKGLALTRRFYGDRRVRYPMVRKGF-KAWADKGFPREKDGRPPKDYFN
    3ir5.1.A  LVTWETQQTDYPRTRPDLPNH------EPRGCPRGASYSWYLYSANRLKYPMMRKRLMKMW-----------REAKALHS

    Target    RARDEWLRLTHEEAADLVAAALINIATTYSGDNGQKLLLQQGYEKEIVEATRGAGTQVLKFRGGMPLLGLTRIFGLYRMA
    3ir5.1.A  DPVEAWASII--EDADK--------AKSFKQARGRGGFVRSSWQ-EVNELIAASNVYTIKNYGPDRVAGFSPI-------

    Target    NSMALLDHKIRGVKPEDALGARGWDNYSWHTDLPPGHPMVTGQQTVDFDLHAVEQARIVVVWGMNWVTTKMPDTHWLTEA
    3ir5.1.A  PAMSMVSYA-SGARYLSLIGGTCLSFYDWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEV

    Target    RLKGTKVVVIACEYSSSSIKADDAIVVRPGTTPALALGLCNVIMRE------KIYDGDYVRRFSDLPLLVRADNLKLLRA
    3ir5.1.A  RYKGTKTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRYTDMPMLV----------

    Target    EEVFGTPQAALKNQTR
    3ir5.1.A  ----------------

### Model #04 | File | Built with | Oligo-State | Ligands | GMQE | QMEANDisCo Global
--- | --- | --- | --- | --- | --- | ---
| PDB | ProMod3 3.2.1 | monomer | None | 0.30 | 0.43 ± 0.05

Template | Seq Identity | Oligo-state | QSQE | Found by | Method | Resolution | Seq Similarity | Range | Coverage | Description
--- | --- | --- | --- | --- | --- | --- | --- | --- | --- | ---
7l5i.1.A | 25.73 | monomer | 0.00 | BLAST | X-ray | 1.73Å | 0.35 | 77 - 377 | 0.59 | Trimethylamine-N-oxide reductase

### Excluded ligands

Ligand Name.Number | Reason for Exclusion | Description
--- | --- | ---
CL.6 | Not biologically relevant. | CHLORIDE ION
EPE.1 | Not biologically relevant. | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID
EPE.2 | Not biologically relevant. | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID
EPE.7 | Not biologically relevant. | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID
MGD.3 | Binding site not conserved. | 2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE GUANOSINE DINUCLEOTIDE
MGD.4 | Binding site not conserved. | 2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE GUANOSINE DINUCLEOTIDE
MO.5 | Binding site not conserved. | MOLYBDENUM ATOM
O.8 | Binding site not conserved. | OXYGEN ATOM

    Target    NNVNRREFLQWIGAAGFSTFALSASNAWGLQAIENPLAAYPNREWEKTYRDLWKSDASFTFLCAPNDTHNCILNAHVRDG
    7l5i.1.A  ----------------------------------------------------------------------------VQDG

    Target    VITRIGPTMKYGEATDLYGSKVTHRWDPRVCQKGLALTRRFYGDRRVRYPMVRKGFKAWADKGFPREKDGRPPKDYFNRA
    7l5i.1.A  KVVKSGPAIEPAVPNELQ----------------TVVADQLYSEARVKCPMVRKGFLANPGK-----------SDTTMRG

    Target    RDEWLRLTHEEAADLVAAALINIATTYSGDNGQKLLLQQGYEKEIVEATRGAGTQVLKFRGGMPLLGLTRIFGLYRMANS
    7l5i.1.A  RDEWVRVSWDEALDLVHNQLKRVRDEH-GSTGIFAGSYGWFSCGSLHASRTLLQRYMNATGG--FVGHK---GDYSTGAA

    Target    MALLDHKIRGVKPEDALGARGWDNYSWHTDLPPGHPMVTGQQTVDFDLHAVEQARIVVVWGMN--------WVTTKMPDT
    7l5i.1.A  QVIMPHVLGTIEVYE-------QQTSWESIL--------------------ESSDIIVLWSANPLTTMRIAWMSTDQKGI

    Target    HWLTEARLKGTKVVVIACEYSSSSIKAD-DAIVVRPGTTPALALGLCNVIMREKIYDGDYVRRFSDLPLLVRADNLKLLR
    7l5i.1.A  EYFKKFQASGKRIICIDPQKSETCQMLNAEWIPVNTATDVPLMLGIAHTLVEQGKHDKDFLKKYTS--------------

    Target    AEEVFGTPQAALKNQTR
    7l5i.1.A  -----------------

## Materials and Methods

## Template Search

Template search with has been performed against the SWISS-MODEL template library (SMTL, last update: 2023-03-23, last included PDB release: 2023-03-17).

## Template Selection

For each identified template, the template's quality has been predicted from features of the target-template alignment. The templates with the highest quality have then been selected for model building.

## Model Building

Models are built based on the target-template alignment using ProMod3 (Studer et al.). Coordinates which are conserved between the target and the template are copied from the template to the model. Insertions and deletions are remodelled using a fragment library. Side chains are then rebuilt. Finally, the geometry of the resulting model is regularized by using a force field.

## Model Quality Estimation

The global and per-residue model quality has been assessed using the QMEAN scoring function (Studer et al.).

## Ligand Modelling

Ligands present in the template structure are transferred by homology to the model when the following criteria are met: (a) The ligands are annotated as biologically relevant in the template library, (b) the ligand is in contact with the model, (c) the ligand is not clashing with the protein, (d) the residues in contact with the ligand are conserved between the target and the template. If any of these four criteria is not satisfied, a certain ligand will not be included in the model. The model summary includes information on why and which ligand has not been included.

## Oligomeric State Conservation

The quaternary structure annotation of the template is used to model the target sequence in its oligomeric form. The method (Bertoni et al.) is based on a supervised machine learning algorithm, Support Vector Machines (SVM), which combines interface conservation, structural clustering, and other template features to provide a quaternary structure quality estimate (QSQE). The QSQE score is a number between 0 and 1, reflecting the expected accuracy of the interchain contacts for a model built based a given alignment and template. Higher numbers indicate higher reliability. This complements the GMQE score which estimates the accuracy of the tertiary structure of the resulting model.

## References

- BLAST
  Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., Madden, T.L. BLAST+: architecture and applications. BMC Bioinformatics 10, 421-430 (2009).
- HHblits
  Steinegger, M., Meier, M., Mirdita, M., Vöhringer, H., Haunsberger, S. J., Söding, J. HH-suite3 for fast remote homology detection and deep protein annotation. BMC Bioinformatics 20, 473 (2019).

## Table T1:

Primary amino acid sequence for which templates were searched and models were built.

NNVNRREFLQWIGAAGFSTFALSASNAWGLQAIENPLAAYPNREWEKTYRDLWKSDASFTFLCAPNDTHNCILNAHVRDGVITRIGPTMKYGEATDLYGS
KVTHRWDPRVCQKGLALTRRFYGDRRVRYPMVRKGFKAWADKGFPREKDGRPPKDYFNRARDEWLRLTHEEAADLVAAALINIATTYSGDNGQKLLLQQG
YEKEIVEATRGAGTQVLKFRGGMPLLGLTRIFGLYRMANSMALLDHKIRGVKPEDALGARGWDNYSWHTDLPPGHPMVTGQQTVDFDLHAVEQARIVVVW
GMNWVTTKMPDTHWLTEARLKGTKVVVIACEYSSSSIKADDAIVVRPGTTPALALGLCNVIMREKIYDGDYVRRFSDLPLLVRADNLKLLRAEEVFGTPQ
AALKNQTR

## Table T2:

Template | Seq Identity | Oligo-state | QSQE | Found by | Method | Resolution | Seq Similarity | Coverage | Description
--- | --- | --- | --- | --- | --- | --- | --- | --- | ---
7b04.1.B | 45.15 | monomer | - | BLAST | X-ray | 2.97Å | 0.43 | 0.96 | Nitrite oxidoreductase subunit A
7b04.2.B | 45.15 | monomer | - | BLAST | X-ray | 2.97Å | 0.43 | 0.96 | Nitrite oxidoreductase subunit A
7b04.1.B | 43.98 | monomer | - | HHblits | X-ray | 2.97Å | 0.42 | 0.94 | Nitrite oxidoreductase subunit A
7b04.2.B | 43.98 | monomer | - | HHblits | X-ray | 2.97Å | 0.42 | 0.94 | Nitrite oxidoreductase subunit A
4ydd.1.A | 31.49 | monomer | - | BLAST | X-ray | 1.86Å | 0.37 | 0.71 | DMSO reductase family type II enzyme, molybdopterin subunit
5e7o.1.A | 31.49 | monomer | - | BLAST | X-ray | 2.40Å | 0.37 | 0.71 | DMSO reductase family type II enzyme, molybdopterin subunit
4ydd.1.A | 30.22 | monomer | - | HHblits | X-ray | 1.86Å | 0.36 | 0.68 | DMSO reductase family type II enzyme, molybdopterin subunit
5e7o.1.A | 29.86 | monomer | - | HHblits | X-ray | 2.40Å | 0.35 | 0.68 | DMSO reductase family type II enzyme, molybdopterin subunit
3ir7.1.A | 27.64 | monomer | - | HHblits | X-ray | 2.50Å | 0.34 | 0.79 | Respiratory nitrate reductase 1 alpha chain
3ir5.1.A | 28.26 | monomer | - | HHblits | X-ray | 2.30Å | 0.35 | 0.79 | Respiratory nitrate reductase 1 alpha chain
1r27.4.A | 27.95 | homo-dimer | 0.16 | HHblits | X-ray | 2.00Å | 0.35 | 0.79 | Respiratory nitrate reductase 1 alpha chain
1q16.1.A | 27.33 | monomer | - | HHblits | X-ray | 1.90Å | 0.34 | 0.79 | Respiratory nitrate reductase 1 alpha chain
3egw.1.A | 29.19 | homo-dimer | 0.15 | HHblits | X-ray | 1.90Å | 0.35 | 0.73 | Respiratory nitrate reductase 1 alpha chain
6sdv.1.A | 18.96 | monomer | - | HHblits | X-ray | 1.90Å | 0.29 | 0.80 | Formate dehydrogenase, alpha subunit, selenocysteine-containing,Formate dehydrogenase, alpha subunit, selenocysteine-containing,W-formate dehydrogenase - alpha subunit
3ir6.1.A | 27.02 | monomer | - | HHblits | X-ray | 2.80Å | 0.34 | 0.79 | Respiratory nitrate reductase 1 alpha chain
6sdr.1.A | 19.27 | monomer | - | HHblits | X-ray | 2.10Å | 0.29 | 0.80 | Formate dehydrogenase, alpha subunit, selenocysteine-containing
2ivf.1.A | 29.84 | monomer | - | HHblits | X-ray | 1.88Å | 0.34 | 0.77 | ETHYLBENZENE DEHYDROGENASE ALPHA-SUBUNIT
3ir5.1.A | 30.49 | monomer | - | BLAST | X-ray | 2.30Å | 0.37 | 0.75 | Respiratory nitrate reductase 1 alpha chain
3ir7.1.A | 30.16 | monomer | - | BLAST | X-ray | 2.50Å | 0.36 | 0.75 | Respiratory nitrate reductase 1 alpha chain
1r27.4.A | 30.16 | homo-dimer | 0.13 | BLAST | X-ray | 2.00Å | 0.36 | 0.75 | Respiratory nitrate reductase 1 alpha chain
3egw.1.A | 30.16 | homo-dimer | 0.13 | BLAST | X-ray | 1.90Å | 0.36 | 0.75 | Respiratory nitrate reductase 1 alpha chain
8bqg.1.A | 18.43 | monomer | - | HHblits | X-ray | 1.95Å | 0.29 | 0.72 | Formate dehydrogenase, alpha subunit, selenocysteine-containing
1q16.1.A | 30.16 | monomer | - | BLAST | X-ray | 1.90Å | 0.36 | 0.75 | Respiratory nitrate reductase 1 alpha chain
3ir6.1.A | 30.16 | monomer | - | BLAST | X-ray | 2.80Å | 0.36 | 0.75 | Respiratory nitrate reductase 1 alpha chain
1e5v.2.A | 24.29 | monomer | - | HHblits | X-ray | 2.40Å | 0.31 | 0.78 | Dimethyl sulfoxide/trimethylamine N-oxide reductase
1e18.1.A | 23.66 | monomer | - | HHblits | X-ray | 2.00Å | 0.31 | 0.78 | DMSO REDUCTASE.
2ivf.1.A | 36.07 | monomer | - | BLAST | X-ray | 1.88Å | 0.38 | 0.69 | ETHYLBENZENE DEHYDROGENASE ALPHA-SUBUNIT
4dmr.1.A | 24.29 | monomer | - | HHblits | X-ray | 1.90Å | 0.31 | 0.78 | DMSO REDUCTASE
1e60.1.A | 24.92 | monomer | - | HHblits | X-ray | 2.00Å | 0.31 | 0.78 | Dimethyl sulfoxide/trimethylamine N-oxide reductase
2iv2.1.A | 19.93 | monomer | - | HHblits | X-ray | 2.27Å | 0.30 | 0.66 | Formate dehydrogenase H
7z0t.1.G | 19.93 | monomer | - | HHblits | EM | NA | 0.30 | 0.66 | Formate dehydrogenase H
1aa6.1.A | 19.93 | monomer | - | HHblits | X-ray | 2.30Å | 0.30 | 0.66 | FORMATE DEHYDROGENASE H
1fdo.1.A | 19.93 | monomer | - | HHblits | X-ray | 2.80Å | 0.30 | 0.66 | FORMATE DEHYDROGENASE H
7l5i.1.A | 25.73 | monomer | - | BLAST | X-ray | 1.73Å | 0.35 | 0.59 | Trimethylamine-N-oxide reductase
7l5s.1.A | 25.73 | monomer | - | BLAST | X-ray | 2.09Å | 0.35 | 0.59 | Trimethylamine-N-oxide reductase
1dms.1.A | 27.06 | monomer | - | BLAST | X-ray | 1.88Å | 0.34 | 0.63 | DMSO REDUCTASE
6q8o.1.C | 19.91 | monomer | - | HHblits | X-ray | 3.61Å | 0.27 | 0.52 | NADH-quinone oxidoreductase subunit 3
3m9s.1.C | 19.91 | monomer | - | HHblits | X-ray | 4.50Å | 0.27 | 0.52 | NADH-quinone oxidoreductase subunit 3
6zjn.1.C | 19.91 | monomer | - | HHblits | EM | NA | 0.27 | 0.52 | NADH-quinone oxidoreductase subunit 3
6ziy.1.C | 19.91 | monomer | - | HHblits | EM | NA | 0.27 | 0.52 | NADH-quinone oxidoreductase subunit 3
7qsd.1.G | 16.91 | monomer | - | HHblits | EM | NA | 0.27 | 0.51 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
6g72.1.G | 16.59 | monomer | - | HHblits | EM | NA | 0.26 | 0.50 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
6zr2.1.G | 16.59 | monomer | - | HHblits | EM | 3.10Å | 0.26 | 0.50 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
2fug.2.C | 19.91 | monomer | - | HHblits | X-ray | 3.30Å | 0.27 | 0.52 | NADH-quinone oxidoreductase chain 3
6zjy.1.C | 19.91 | monomer | - | HHblits | EM | NA | 0.27 | 0.52 | NADH-quinone oxidoreductase subunit 3
7ak6.1.G | 16.59 | monomer | - | HHblits | EM | NA | 0.26 | 0.50 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
7vxu.1.L | 15.61 | monomer | - | HHblits | EM | NA | 0.27 | 0.50 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
6zjl.1.C | 19.91 | monomer | - | HHblits | EM | NA | 0.27 | 0.52 | NADH-quinone oxidoreductase subunit 3
6qc5.1.C | 16.59 | monomer | - | HHblits | EM | NA | 0.27 | 0.50 | NADH:ubiquinone oxidoreductase core subunit S1
6qcf.1.C | 16.59 | monomer | - | HHblits | EM | NA | 0.27 | 0.50 | NADH:ubiquinone oxidoreductase core subunit S1

The table above shows the top 50 filtered templates. A further 330 templates were found which were considered to be less suitable for modelling than the filtered list.
1aa6.1.A, 1be3.1.E, 1bgy.1.P, 1dms.1.A, 1e18.1.A, 1e5v.2.A, 1e60.1.A, 1eiw.1.A, 1eu1.1.A, 1fdo.1.A, 1fxk.1.C, 1g8j.1.A, 1g8k.1.A, 1h0h.1.A, 1ici.1.A, 1jeo.1.A, 1kb9.1.E, 1kqf.1.A, 1l0l.1.E, 1l0n.1.E, 1m2g.1.A, 1m2h.1.A, 1m2j.1.A, 1m2k.1.A, 1m2n.1.A, 1m2n.1.B, 1ma3.1.A, 1nri.1.A, 1ntk.1.E, 1ntm.1.E, 1ogy.1.A, 1q16.1.A, 1r27.4.A, 1s5p.1.A, 1s7g.1.A, 1s7g.1.B, 1s7g.1.C, 1s7g.1.D, 1s7g.1.E, 1sqb.1.E, 1sqp.1.P, 1sqq.1.P, 1sqv.1.E, 1tk9.1.A, 1tmo.1.A, 1tzb.1.A, 1tzb.1.B, 1vf5.1.D, 1vf5.1.L, 1vim.1.A, 1vim.1.D, 1viv.1.A, 1x94.1.A, 1x94.1.B, 1yc5.1.A, 1zrt.1.C, 1zrt.1.F, 2a3n.1.A, 2b4y.1.A, 2b4y.3.A, 2d2c.1.D, 2d2c.1.L, 2e75.1.D, 2e76.1.D, 2e7z.1.A, 2fug.2.C, 2fyn.1.C, 2fyn.2.C, 2fyu.1.E, 2h2i.1.A, 2h4h.1.A, 2h59.1.B, 2i2w.1.A, 2i2w.2.B, 2iv2.1.A, 2ivf.1.A, 2nya.1.A, 2nyr.1.A, 2nyr.1.B, 2pq4.1.B, 2qjk.1.C, 2qjp.1.C, 2qjy.3.F, 2v3v.1.A, 2v45.1.A, 2v4m.1.A, 2vpx.1.D, 2vpz.1.A, 2x3y.1.A, 2xbl.1.A, 2ybb.1.b, 2yva.1.A, 2zdi.1.C, 2zj4.1.A, 3egw.1.A, 3etn.1.A, 3eua.1.A, 3hba.1.A, 3hba.1.B, 3ir5.1.A, 3ir6.1.A, 3ir7.1.A, 3jr3.1.A, 3jwp.1.A, 3k35.1.A, 3m9s.1.C, 3o5a.1.A, 3pki.1.A, 3riy.2.A, 3sho.1.A, 3sho.1.C, 3u31.1.A, 3zg6.1.A, 4aay.1.A, 4bv2.3.A, 4d6t.1.E, 4dmr.1.A, 4g1c.1.A, 4g1c.2.A, 4h44.1.D, 4hda.1.A, 4hda.2.A, 4ivn.1.A, 4jja.1.A, 4lzj.1.A, 4lzj.1.B, 4lzj.2.B, 4m0d.1.A, 4m0d.2.A, 4m0d.2.B, 4ogq.1.L, 4pv1.1.L, 4s12.1.A, 4s12.2.A, 4s12.2.B, 4twi.1.A, 4twj.1.A, 4utn.1.A, 4utn.2.A, 4v4c.1.A, 4ydd.1.A, 5bwl.1.A, 5e7o.1.A, 5gpn.17.A, 5gpn.24.A, 5gpn.5.A, 5j8k.55.A, 5kkz.1.C, 5kli.1.C, 5klv.1.P, 5ltz.1.A, 5lu5.1.A, 5lu6.1.A, 5lu7.1.A, 5mf6.1.A, 5nmi.1.E, 5nqd.1.A, 5o31.1.8, 5oj7.1.A, 5ojn.1.A, 5okd.1.E, 5t5i.1.B, 5x16.1.A, 5xhs.1.A, 5xtb.1.L, 5xte.1.C, 5y2f.1.A, 6aco.1.A, 6acp.1.A, 6adq.1.L, 6btm.1.B, 6cz7.1.A, 6enx.1.A, 6eo0.1.A, 6eqs.3.A, 6f0k.1.B, 6fky.1.A, 6fky.2.A, 6flg.1.A, 6fo0.1.E, 6fo6.1.J, 6g72.1.G, 6gcs.1.A, 6giq.1.E, 6hu9.1.E, 6hwh.1.A, 6ljk.1.A, 6ljm.1.A, 6lod.1.B, 6nhg.1.E, 6nr8.1.C, 6nr8.1.E, 6nr9.1.S, 6nr9.1.U, 6nrb.1.S, 6nrb.1.U, 6nrc.1.C, 6nrc.1.E, 6nrd.1.S, 6nrd.1.U, 6o1x.1.A, 6o1x.2.A, 6o1y.1.A, 6o1y.2.A, 6q8o.1.C, 6q9e.1.E, 6q9e.1.O, 6qbx.15.A, 6qbx.5.A, 6qc2.33.A, 6qc2.43.A, 6qc3.15.A, 6qc3.5.A, 6qc4.15.A, 6qc4.5.A, 6qc5.1.C, 6qcf.1.C, 6r4i.1.A, 6r4i.1.B, 6rfq.1.A, 6rfs.1.A, 6rqf.1.D, 6rqf.1.L, 6rxj.1.A, 6rxm.1.A, 6rxm.2.A, 6rxm.3.A, 6rxm.4.A, 6rxm.5.A, 6rxm.6.A, 6rxo.1.A, 6rxo.2.A, 6rxp.2.A, 6rxq.4.A, 6rxs.1.A, 6s6y.1.B, 6sdr.1.A, 6sdv.1.A, 6t0b.1.E, 6t0b.1.O, 6t15.1.E, 6t15.1.O, 6tg9.1.A, 6vy1.1.A, 6vy1.1.G, 6vy1.1.M, 6x89.1.H, 6xvg.3.A, 6yj4.1.G, 6ymx.1.Q, 6ymx.1.Z, 6ziy.1.C, 6zjl.1.C, 6zjn.1.C, 6zjy.1.C, 6zk9.1.C, 6zmk.1.A, 6zmk.1.B, 6zr2.1.G, 7a23.1.O, 7ak5.1.G, 7ak6.1.G, 7aqr.1.F, 7ar7.1.G, 7ar8.1.G, 7arc.1.F, 7b04.1.B, 7b04.2.B, 7bkb.1.F, 7bkb.1.L, 7cl0.1.A, 7dgr.10.A, 7dgr.60.A, 7dgs.50.A, 7dgs.60.A, 7e1v.1.P, 7e5z.1.A, 7en5.1.A, 7en6.1.A, 7en6.1.B, 7en6.1.C, 7en6.1.D, 7jrg.1.E, 7l5i.1.A, 7l5s.1.A, 7nz1.1.E, 7o37.1.E, 7o37.1.O, 7o3c.1.E, 7o3c.1.O, 7o3h.1.E, 7o3h.1.O, 7p61.1.C, 7p63.1.C, 7q5y.1.A, 7qrm.1.D, 7qsd.1.G, 7qv7.1.L, 7qv7.1.O, 7r0w.1.L, 7r0w.1.Q, 7rh5.1.V, 7rja.1.H, 7rjb.1.I, 7t2r.1.A, 7t30.1.A, 7tce.2.F, 7tgh.58.A, 7tlj.1.C, 7tz6.1.E, 7tz6.1.P, 7v2c.1.L, 7vw6.1.A, 7vxu.1.L, 7z0t.1.G, 7zd6.1.4, 7zm7.1.I, 7zxy.1.D, 7zxy.1.L, 8asi.1.A, 8asi.1.E, 8asj.1.E, 8b9z.1.G, 8ba0.1.G, 8bel.1.B, 8bel.1.I, 8bpx.51.A, 8bqg.1.A, 8e73.55.A, 8e9g.1.G