{"text_format":"3","workbook":"xls","n_sheets":2,"sheets":["Lab_primer_list","Wild_primer_list"],"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Lab_primer_list","index":0,"n_rows":50,"n_cols":7,"rows_kept":50,"flags":["formulas_cached_only"]}
["Lab primer list"]
["Primer_name","Name_F","SequenceF","Name_R","SequenceR","Gen","Target"]
["16S.1100.F16_100_for.1492R_100_rev","16S.1100.F16_100_for","CAACGAGCGCAACCCT","1492R_100_rev","TACGGYTACCTTGTTAYGACTT","16S","Bacteria"]
["18S_0067a_deg_3Mod_53_F.NSR399_3Mod_53_R","18S_0067a_deg_3Mod_53_F","AAGCCATGCATGYCTAAGTATM","NSR399_3Mod_53_R","TCTCAGGCTCCYTCTCC","18S","Eukaryota"]
["18S_0067a_deg_5Mod_52_F.NSR399_5Mod_52_R","18S_0067a_deg_5Mod_52_F","GCCATGCATGYCTAAGTATMA","NSR399_5Mod_52_R","AGGCTCCYTCTCCGG","18S","Eukaryota"]
["18S9modF_88_F.18S637modR_89_R","18S9modF_88_F","ATCCTGCCAGTAGTCATATGCTTG","18S637modR_89_R","TACGCTWYTGGAGCTGGAGTTACCG","18S","Eukaryota"]
["27M_F_98_F.CCR_98_R","27M_F_98_F","AGRGTTTGATCMTGGCTCAG","CCR_98_R","GCTGCCTCCCGTAGGAGTCTGG","16S","Bacteria"]
["27M_F_98_F.Klin0341_CR_18_R","27M_F_98_F","AGRGTTTGATCMTGGCTCAG","Klin0341_CR_18_R","CTGCWGCCNCCCGTAGG","16S","Bacteria"]
["5.8SR_130_F.ITS4_130_R","5.8SR_130_F","TCGATGAAGAACGCAGCG","ITS4_130_R","TCCTCCGCTTATTGATATGC","ITS","Eukaryota"]
["515F_Y_118_F.806R_118_R","515F_Y_118_F","GTGYCAGCMGCCGCGGTAA","806R_118_R","GGACTACNVGGGTWTCTAAT","16S","Bacteria"]
["519R_F_119_F.CDR_119_R","519R_F_119_F","CAGCMGCCGCGGTAATWC","CDR_119_R","CTTGTGCGGGCCCCCGTCAATTC","16S","Bacteria"]
["ACM_008_17_F.ADM330_CR_17_R","ACM_008_17_F","AGAGTTTGATCCTGGCTCAG","ADM330_CR_17_R","GCTGCCTCCCGTAGGAGT","16S","Bacteria"]
["ADM330_18_F.Klin0785_CR_19_R","ADM330_18_F","ACTCCTACGGGAGGCAGC","Klin0785_CR_19_R","GACTACHVGGGTATCTAATCC","16S","Bacteria"]
["D3A_5Mod_46_F.D3B_5Mod_46_R","D3A_5Mod_46_F","CCGTCTTGAAACACGGA","D3B_5Mod_46_R","CGGAAGGAACCAGCTACTA","28S","Eukaryota"]
["Df_ITSRev_114_F.Df_ITSnesFor_114_R","Df_ITSRev_114_F","GCGGGTCTTCCTATATAAACAAGAACC","Df_ITSnesFor_114_R","ATACGTCCCTGCCCTTTGTA","ITS","Eukaryota"]
["Eim_Ap5_F_60_F.Eim_Ap5_R_60_R","Eim_Ap5_F_60_F","YAAAGGAATTTGAATCCTCGTTT","Eim_Ap5_R_60_R","YAGAATTGATGCCTGAGYGGTC","tRNA","Eimeria"]
["Eim_COX0_50_F.Eim_COX0_50_R","Eim_COX0_50_F","CCTCATTTAACGGTGATCCAGTACT","Eim_COX0_50_R","GCACCAAGAGATAGTACGAAATGGA","COX0","Eimeria"]
["Eim_COX1_51_F.Eim_COX1_51_R","Eim_COX1_51_F","CTCCAACCTCAGTCGATTTAATTGTAT","Eim_COX1_51_R","TCATCATATGGTGTGCCCAAACTA","COX1","Eimeria"]
["Eim_Nuc3665_17_F.Eim_Nuc3666_17_R","Eim_Nuc3665_17_F","AGTTGTAGTTCGGGAGGTCTTC","Eim_Nuc3666_17_R","GAGGTAGTCGCCACCCAGA","EfaB_31746","Eimeria"]
["Eim_Nuc6133_28_F.Eim_Nuc6134_28_R","Eim_Nuc6133_28_F","TCTGGGAAGATGGGGTGTACA","Eim_Nuc6134_28_R","TGCGTTACGGTGTATAGGCA","EfaB_31746","Eimeria"]
["Eim_ORFZ_131_F.Eim_ORFVH_131_R","Eim_ORFZ_131_F","GATGATATATCTTATTATTCAATTCCTT","Eim_ORFVH_131_R","CCACGATACCAATTTTGTACTGT","ORF470","Eimeria"]
["Em_WG_MT_4506F_53Mod_83_F.Em_WG_MT_4993R_53Mod_84_R","Em_WG_MT_4506F_53Mod_83_F","GTTATTAGTACTGAGTTACTAC","Em_WG_MT_4993R_53Mod_84_R","GAGGTAATACCATAATACTAG","COIII","Coccidia"]
["Em_WG_MTInvF_66_F.COI_1202R_66_R","Em_WG_MTInvF_66_F","ACTGGATCACCATTAAAGGCAGCA","COI_1202R_66_R","CCAAKRAYHGCACCAAGAGATA","COI","Eukaryota"]
["EntOv_430F_117_F.EntUng_900R_117_R","EntOv_430F_117_F","GTAGTGACGACAAATAACTCTTG","EntUng_900R_117_R","TTTCGTTCTTGATTAATGAACG","18S","Eukaryota"]
["F1073_101_F.1492R_100_R","F1073_101_F","CATGGYTGTCGTCAGCTCGT","1492R_100_R","TACGGYTACCTTGTTAYGACTT","16S","Bacteria"]
["fwhF2_106_F.fwhR2n_106_R","fwhF2_106_F","GGDACWGGWTGAACWGTWTAYCCHCC","fwhR2n_106_R","GTRATWGCHCCDGCTARWACWGG","COI","Eukaryota"]
["Gen_600_7_F.Hadz_1200_5_R","Gen_600_7_F","GCCGCGGTAATTCCAGCT","Hadz_1200_5_R","CCCGTGTTGAGTCAAATTAAGC","18S","Eukaryota"]
["Gen_600_7_F.Mach2Gen_7_R","Gen_600_7_F","GCCGCGGTAATTCCAGCT","Mach2Gen_7_R","TCCGTCAATTTCTTTAAGTTTCAG","18S","Eukaryota"]
["Gen_600_7_F.Nem_0425_4_2_R","Gen_600_7_F","GCCGCGGTAATTCCAGCT","Nem_0425_4_2_R","ARACATTCTTGGCAAATGCYTTC","18S","Eukaryota"]
["Hadz_566_6_F.Mach2Gen_7_R","Hadz_566_6_F","CAGCAGCCGCGGTAATTCC","Mach2Gen_7_R","TCCGTCAATTTCTTTAAGTTTCAG","18S","Eukaryota"]
["Hadz_566_6_F.Nem_0425_4_2_R","Hadz_566_6_F","CAGCAGCCGCGGTAATTCC","Nem_0425_4_2_R","ARACATTCTTGGCAAATGCYTTC","18S","Eukaryota"]
["HSPF3_112_F.HSPR3_112_R","HSPF3_112_F","GCTGSTGATACTCACTTGGGTGG","HSPR3_112_R","CTCTTGTCCATACCAGCATCC","HSP70","Eukaryota"]
["ITS1_129_F.ITS2_129_R","ITS1_129_F","TCCGTAGGTGAACCTGCGG","ITS2_129_R","GCTGCGTTCTTCATCGATGC","ITS","Eukaryota"]
["Klin0341_19_F.Klin0785_CR_19_R","Klin0341_19_F","CCTACGGGNGGCWGCAG","Klin0785_CR_19_R","GACTACHVGGGTATCTAATCC","16S","Bacteria"]
["Klin0341_19_for.CS2_907R_101_rev","Klin0341_19_for","CTACGGGNGGCWGCAG","CS2_907R_101_rev","CCGTCAATTCMTTTRAGTTT","16S","Bacteria"]
["LSU_Fwd_2_3Mod_55_F.LSU_R_4_54_R","LSU_Fwd_2_3Mod_55_F","ACAAGTACCDTRAGGGAAAGTT","LSU_R_4_54_R","GTTAGACTYCTTGGTCCGTG","28S","Eukaryota"]
["Mach1_9_F.Mach2Gen_7_R","Mach1_9_F","TGGTGCCAGCAGCCGC","Mach2Gen_7_R","TCCGTCAATTTCTTTAAGTTTCAG","18S","Eukaryota"]
["MarkN_10_F.Euk360_CR_21_R","MarkN_10_F","GCTTGTCTCAAAGATTAAGCCN","Euk360_CR_21_R","TCTCAKGCKCCYTCTCCG","18S","Eukaryota"]
["MarkN_10_F.Proti440R_28_R","MarkN_10_F","GCTTGTCTCAAAGATTAAGCCN","Proti440R_28_R","CAGGCYCSCTCTCCGGA","18S","Eukaryota"]
["Nem_0425_6CR_14_F.EukB_MH_24_R","Nem_0425_6CR_14_F","ATAACAGGTCTGTGATGCCCTT","EukB_MH_24_R","TGATCCTTCTGCAGGTTCACC","18S","Eukaryota"]
["NLF184cw _74_F.NL818cw_74_R","NLF184cw _74_F","TACCCGCTGAAYTTAAGCATAT","NL818cw_74_R","AGACTCCTTGGTCCRTGT","28S","Eukaryota"]
["Prot1702_32_F.EukB_MH_24_R","Prot1702_32_F","YGGTGRTGCATGGCCGYT","EukB_MH_24_R","TGATCCTTCTGCAGGTTCACC","18S","Eukaryota"]
["Prot1702_32_F.EukB_MV_25_R","Prot1702_32_F","YGGTGRTGCATGGCCGYT","EukB_MV_25_R","TCCTTCTGCAGGTTCACCTAC","18S","Eukaryota"]
["Prot1702_32_F.wang1624CR6S_16_R","Prot1702_32_F","YGGTGRTGCATGGCCGYT","wang1624CR6S_16_R","GGGCGGTGTGTACAAAGG","18S","Eukaryota"]
["Proti15_25_F.Proti440R_28_R","Proti15_25_F","TGCCAGTAGTCATATGCTTGTYT","Proti440R_28_R","CAGGCYCSCTCTCCGGA","18S","Eukaryota"]
["Proti1580_33_F.wang1624CR6S_16_R","Proti1580_33_F","TTGACGGARKGGYACCACMA","wang1624CR6S_16_R","GGGCGGTGTGTACAAAGG","18S","Eukaryota"]
["Proti628_30_F.wang1141CR_14_R","Proti628_30_F","GTCTGGTGCCAGCASCC","wang1141CR_14_R","GGTGCCCTTCCGTCAATTC","18S","Eukaryota"]
["Proti643_31_F.Proti1580R_32_R","Proti643_31_F","CCGCGGTAATTCCAGCTC","Proti1580R_32_R","TKGTGGTRCCMYTCCGTCAA","18S","Eukaryota"]
["Proti643_31_F.wang1141CR_14_R","Proti643_31_F","CCGCGGTAATTCCAGCTC","wang1141CR_14_R","GGTGCCCTTCCGTCAATTC","18S","Eukaryota"]
["wang1141_13_F.Nem_0425_6_3_R","wang1141_13_F","GAATTGACGGAAGGGCACC","Nem_0425_6_3_R","AAGGGCATCACAGACCTGTTAT","18S","Eukaryota"]

{"sheet":"Wild_primer_list","index":1,"n_rows":50,"n_cols":7,"rows_kept":50,"flags":["formulas_cached_only"]}
[]
["Primer_name","Name_F","SequenceF","Name_R","SequenceR","Gen","Target"]
["18S_0067a_deg_3Mod_53_F.NSR399_3Mod_53_R","18S_0067a_deg_3Mod_53_for","AAGCCATGCATGYCTAAGTATM","NSR399_3Mod_53_rev","TCTCAGGCTCCYTCTCC","18S","Eukaryota"]
["18S_0067a_deg_5Mod_52_F.NSR399_5Mod_52_R","18S_0067a_deg_5Mod_52_for","GCCATGCATGYCTAAGTATMA","NSR399_5Mod_52_rev","AGGCTCCYTCTCCGG","18S","Eukaryota"]
["18S9modF_88_F.18S637modR_89_R","18S9modF_88_for","ATCCTGCCAGTAGTCATATGCTTG","18S637modR_89_rev","TACGCTWYTGGAGCTGGAGTTACCG","18S","Eukaryota"]
["27M_F_98_F.CCR_98_R","27M_F_98_for","AGRGTTTGATCMTGGCTCAG","CCR_98_rev","GCTGCCTCCCGTAGGAGTCTGG","16S","Bacteria"]
["5.8SR_130_F.ITS4_130_R","5.8SR_130_for","TCGATGAAGAACGCAGCG","ITS4_130_rev","TCCTCCGCTTATTGATATGC","ITS","Eukaryota"]
["515F_Y_118_F.806R_118_R","515F_Y_118_for","GTGYCAGCMGCCGCGGTAA","806R_118_rev","GGACTACNVGGGTWTCTAAT","16S","Bacteria"]
["519R_F_119_F.CDR_119_R","519R_F_119_for","CAGCMGCCGCGGTAATWC","CDR_119_rev","CTTGTGCGGGCCCCCGTCAATTC","16S","Bacteria"]
["ACM_008_17_F.ADM330_CR_17_R","ACM_008_17_for","AGAGTTTGATCCTGGCTCAG","ADM330_CR_17_rev","GCTGCCTCCCGTAGGAGT","16S","Bacteria"]
["AL3544_113_F.AL3545_113_R","AL3544_113_for","CCCTTCATCGGIGGTAACTT","AL3545_113_rev","GTGGCCACCACICCCGTGCC","TPI","Metazoa"]
["BhRDr_115_F.RD5_115_R","BhRDr_115_for","GAGCTTTTTAACTGCAACAACG","RD5_115_rev","ATCTGGTTGATCCTGCCAGT","18S","Eukaryota"]
["COX1.DINO.F_122_F.COX1.DINO.2.R_122_R","COX1.DINO.F_122_for","GAATTTGGAGGTGGCACNGGNTGGACNYT","COX1.DINO.2.R_122_rev","CCCATCGTATACATRTGRTGNCCCCANAC","COI","Eukaryota"]
["D3A_5Mod_46_F.D3B_5Mod_46_R","D3A_5Mod_46_for","CCGTCTTGAAACACGGA","D3B_5Mod_46_rev","CGGAAGGAACCAGCTACTA","28S","Eukaryota"]
["Df_ITSRev_114_F.Df_ITSnesFor_114_R","Df_ITSRev_114_for","GCGGGTCTTCCTATATAAACAAGAACC","Df_ITSnesFor_114_rev","ATACGTCCCTGCCCTTTGTA","ITS","Eukaryota"]
["EntOv_430F_117_F.EntUng_900R_117_R","EntOv_430F_117_for","GTAGTGACGACAAATAACTCTTG","EntUng_900R_117_rev","TTTCGTTCTTGATTAATGAACG","18S","Eukaryota"]
["F1073_101_F.1492R_100_R","F1073_101_for","CATGGYTGTCGTCAGCTCGT","1492R_100_rev","TACGGYTACCTTGTTAYGACTT","16S","Bacteria"]
["fwhF2_106_F.fwhR2n_106_R","fwhF2_106_for","GGDACWGGWTGAACWGTWTAYCCHCC","fwhR2n_106_rev","GTRATWGCHCCDGCTARWACWGG","COI","Eukaryota"]
["Gen_600_7_F.Hadz_1200_5_R","Gen_600_7_for","GCCGCGGTAATTCCAGCT","Hadz_1200_5_rev","CCCGTGTTGAGTCAAATTAAGC","18S","Eukaryota"]
["Gen_600_7_F.Mach2Gen_7_R","Gen_600_7_for","GCCGCGGTAATTCCAGCT","Mach2Gen_7_rev","TCCGTCAATTTCTTTAAGTTTCAG","18S","Eukaryota"]
["Gen_600_7_F.Nem_0425_4_2_R","Gen_600_7_for","GCCGCGGTAATTCCAGCT","Nem_0425_4_2_rev","ARACATTCTTGGCAAATGCYTTC","18S","Eukaryota"]
["Hadz_566_6_F.Nem_0425_4_2_R","Hadz_566_6_for","CAGCAGCCGCGGTAATTCC","Nem_0425_4_2_rev","ARACATTCTTGGCAAATGCYTTC","18S","Eukaryota"]
["Hadz_566_6_F.Mach2Gen_7_R","Hadz_566_6_for","CAGCAGCCGCGGTAATTCC","Mach2Gen_7_rev","TCCGTCAATTTCTTTAAGTTTCAG","18S","Eukaryota"]
["HSPF3_112_F.HSPR3_112_R","HSPF3_112_for","GCTGSTGATACTCACTTGGGTGG","HSPR3_112_rev","CTCTTGTCCATACCAGCATCC","HSP70","Eukaryota"]
["ITS1_129_F.ITS2_129_R","ITS1_129_for","TCCGTAGGTGAACCTGCGG","ITS2_129_rev","GCTGCGTTCTTCATCGATGC","ITS","Eukaryota"]
["Klin0341_19_F.Klin0785_CR_19_R","Klin0341_19_for","CCTACGGGNGGCWGCAG","Klin0785_CR_19_rev","GACTACHVGGGTATCTAATCC","16S","Bacteria"]
["LepF1_120_F.M_LepF1_R_120_R","LepF1_120_for","ATTCAACCAATCATAAAGATATTGG","M_LepF1_Rev_120_rev","CGTGGAAAWGCTATATCWGGTG","COI","Eukaryota"]
["LSU_Fwd_2_3Mod_55_F.LSU_Rev_4_54_R","LSU_Fwd_2_3Mod_55_for","ACAAGTACCDTRAGGGAAAGTT","LSU_Rev_4_54_rev","GTTAGACTYCTTGGTCCGTG","28S","Eukaryota"]
["Mach1_9_F.Mach2Gen_7_R","Mach1_9_for","TGGTGCCAGCAGCCGC","Mach2Gen_7_rev","TCCGTCAATTTCTTTAAGTTTCAG","18S","Eukaryota"]
["MarkN_10_F.Euk360_CR_21_R","MarkN_10_for","GCTTGTCTCAAAGATTAAGCCN","Euk360_CR_21_rev","TCTCAKGCKCCYTCTCCG","18S","Eukaryota"]
["MarkN_10_F.Proti440R_28_R","MarkN_10_for","GCTTGTCTCAAAGATTAAGCCN","Proti440R_28_rev","CAGGCYCSCTCTCCGGA","18S","Eukaryota"]
["mlCOIintF_121_F.Fol_degen_R_121_R","mlCOIintF_121_for","GGWACWGGWTGAACWGTWTAYCCYCC","Fol_degen_rev_121_rev","TANACYTCNGGRTGNCCRAARAAYCA","COI","Eukaryota"]
["mlCOIintF_XT_104_F.JgHCO2198_104_R","mlCOIintF_XT_104_for","GGWACWRGWTGRACWITITAYCCYCC","JgHCO2198_104_rev","TAIACYTCIGGRTGICCRAARAAYCA","COI","Eukaryota"]
["NdmlCOIlintF_1_72_F.dgHCO2198_72_R","NdmlCOIlintF_1_72_for","GGAACAGGATGAACTGTTTACCCTCC","dgHCO2198_72_rev","TAAACTTCAGGGTGACCAAARAAYCA","COI","Eukaryota"]
[null,"Nem_0425_6CR_14_for","ATAACAGGTCTGTGATGCCCTT","EukB_MH_24_rev","TGATCCTTCTGCAGGTTCACC","18S","Eukaryota"]
["NLF184cw_74_F.NL818cw_74_R","NLF184cw _74_for","TACCCGCTGAAYTTAAGCATAT","NL818cw_74_rev","AGACTCCTTGGTCCRTGT","28S","Eukaryota"]
["Prot1702_32_F.EukB_MH_24_R","Prot1702_32_for","YGGTGRTGCATGGCCGYT","EukB_MH_24_rev","TGATCCTTCTGCAGGTTCACC","18S","Eukaryota"]
["Prot1702_32_F.EukB_MV_25_R","Prot1702_32_for","YGGTGRTGCATGGCCGYT","EukB_MV_25_rev","TCCTTCTGCAGGTTCACCTAC","18S","Eukaryota"]
["Prot1702_32_F.wang1624CR6S_16_R","Prot1702_32_for","YGGTGRTGCATGGCCGYT","wang1624CR6S_16_rev","GGGCGGTGTGTACAAAGG","18S","Eukaryota"]
["Proti15_25_F.Proti440R_28_R","Proti15_25_for","TGCCAGTAGTCATATGCTTGTYT","Proti440R_28_rev","CAGGCYCSCTCTCCGGA","18S","Eukaryota"]
["Proti1580_33_F.wang1624CR6S_16_R","Proti1580_33_for","TTGACGGARKGGYACCACMA","wang1624CR6S_16_rev","GGGCGGTGTGTACAAAGG","18S","Eukaryota"]
["Proti628_30_F.wang1141CR_14_R","Proti628_30_for","GTCTGGTGCCAGCASCC","wang1141CR_14_rev","GGTGCCCTTCCGTCAATTC","18S","Eukaryota"]
["Proti643_31_F.Proti1580R_32_R","Proti643_31_for","CCGCGGTAATTCCAGCTC","Proti1580R_32_rev","TKGTGGTRCCMYTCCGTCAA","18S","Eukaryota"]
["Proti643_31_F.wang1141CR_14_R","Proti643_31_for","CCGCGGTAATTCCAGCTC","wang1141CR_14_rev","GGTGCCCTTCCGTCAATTC","18S","Eukaryota"]
["wang1141_13_F.Nem_0425_6_3_R","wang1141_13_for","GAATTGACGGAAGGGCACC","Nem_0425_6_3_rev","AAGGGCATCACAGACCTGTTAT","18S","Eukaryota"]
["GDH3_133_F.GHD4_133_R","GDH3_133_for","ATGACYGAGCTYCAGAGGCACGT","GHD4_133_rev","GTGGCGCARGGCATGATGCA","GDH","Giardia"]
["54F_134_F.539R_134_R","54F_134_for","CAYGTCTAAGTATAAATAACTAC","539R_134_rev","CTTGCCCTCYAATCGTWCT","18S","Ciliates"]
["Bgf_132_F.Bgr_132_R","BGf _132_for","GAACGAACGAGATCGAGGTCCG","Bgr_132_rev","CTCGACGAGCTTCGTGTT","BG","Giardia"]
["Eim_Ap5_F_60_F.Eim_Ap5_R_60_R","Eim_Ap5_60_for","YAAAGGAATTTGAATCCTCGTTT","Eim_Ap5_60_rev","YAGAATTGATGCCTGAGYGGTC","tRNA","Eimeria"]
["Eim_ORFZ_131_F.Eim_ORFVH_131_R","Eim_ORFZ_131_F","GATGATATATCTTATTATTCAATTCCTT","Eim_ORFVH_131_R","CCACGATACCAATTTTGTACTGT","ORF470","Eimeria"]