;______________________________________________________________________
[task]
   task = fit
   data = progress
[mechanism]
   E + S <==> E.S       :     kaS      kdS
   E.S ---> E + P       :     kdP
   E + I <==> E.I       :     ka       kd
[constants]
   kaS = 1
   kdS = 31
   kdP = 0.1 ??
   ka = 0.1 ??
   kd = 0.0001 ??
[concentrations]
   E = 0.08 ??
   S = 20
[responses]
   P = 88
[data]
   directory ^/data
graph PF-07321332 :: [E] titration :: rep. 2
   monitor E, E.S, E.I
   offset -1 ? local
   sheet PF332-R2-f.csv
   column	11	| conc I =	0.00000000	| label	0 nM [I]
   column	10	| conc I =	0.01734153	| label	17	
   column	9	| conc I =	0.026012295	| label	26	
   column	8	| conc I =	0.039018442	| label	39	
   column	7	| conc I =	0.058527663	| label	59	
   column	6	| conc I =	0.087791495	| label	88	
   column	5	| conc I =	0.131687243	| label	132	
   column	4	| conc I =	0.197530864	| label	198	
   column	3	| conc I =	0.296296296	| label	296	
   column	2	| conc I =	0.444444444	| label	444	
[output]
   directory ^/output/fit-R2-001
[settings]
{Output}
   XAxisLabel = t (s)
   YAxisLabel = {/Symbol D}F (rfu)
{Fit}
   SquaresIncreasePercent = 5
   PlotSearchCorrelations = y
[end]
