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["Table S1 Primers used in this study"]
["No.","Primer name","Sequence (5'-3')","Purpose"]
[1,"OBP1-gRNA","GAAATTAATACGACTCACTATAGGGATATTGTGCGTCACGACGgttttagagctagaaatagc","sgRNA synthsis"]
[null,"CRISR-R","AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTgctatttctagctctaaaac"]
[2,"OBP1-MF","TTTCGGTAGGGTTTGTTTGTG","Mutation detection"]
[null,"OBP1-MR","GAATTGGCTTCATGGCTTCG"]
[3,"Oligo 1 ","AAAACGACGGCCAGTGAATTCCATAATTATTTCCAACCATGAAC","Donor construction"]
[null,"Oligo 2 ","AATTAGATCCACGTGGCGTCGTGTCGGC"]
[4,"Oligo 3 ","GACGCCACGTGGATCTAATTCAATTAGAGAC"]
[null,"Oligo 4 ","GTGCGTCACGTTAAGATACATTGATGAGTTTGGAC"]
[5,"Oligo 5 ","TGTATCTTAACGTGACGCACAATATCCTCCG"]
[null,"Oligo 6","GACCATGATTACGCCAAGCTTAAAGTGGTGCACCGAGGTCTC"]
[6,"OBP1-LF","CTGAGCGTTGGGACCATTCT","Amplification of knock-in left arm "]
[null,"OBP1-LR","TTCTGCTTGTCGGCCATGAT"]
[7,"OBP1-RF","AGATCCGCCACAACATCGAG","Amplification of knock-in right arm "]
[null,"OBP1-RR","GTTGATGTCTGTTGACCGGC"]
[8,"OBP1-F","GGTCCGTTTCGGGGGTTAAT","Fluorescent cassette amplification"]
[null,"OBP1-R","GTTTTCTCTTCCGCCGTTCG"]
[9,"OBP1OE-1F","ATGATCATCATGACAGATCTTTCCATCGTTGGTCCTACTGAG","Amplification of 4,000 bp sequence immediately upstream of the OBP1 ATG translation start codon"]
[null,"OBP1OE-1R","CCCTACCGAAAACATGGTGGCTACTCTCTGTGTG"]
[10,"OBP1OE-2F","CAGAGAGTAGCCACCATGTTTTCGGTAGGGTTTGTTTGTG","Amplification of OBP1 CDS"]
[null,"OBP1OE-2R","AAGCTTGCCTGATCCTACTAGGAAATAGTGCTTCGGATC"]
[11,"OBP1OE-3F","CACTATTTCCTAGTAGGATCAGGCAAGCTTGAGA","Amplification of T2A-EGFP"]
[null,"OBP1OE-3R","AAAAAGGGCAGCCCCTTACTTGTACAGCTCGTCCATG"]
[12,"OBP1OE-4F","GAGCTGTACAAGTAAGGGGCTGCCCTTTTTACTGTAT","Amplification of OBP1 3’ UTR"]
[null,"OBP1OE-4R","CACTGAACATTGTCAGATCTTGAGGTTATTCAAAGGTTTTGGGAC"]
[13,"Reverse-L1","GAAGTGCAGCCAACGTCAAG","Amplification of overexpression left arm "]
[null,"Reverse-L2","TTTGACTCACGCGGTCGTTA"]
[14,"Reverse-L3","TCAAAGTCCACGAGGCGTAG","Nested amplification of overexpression left arm "]
[null,"Reverse-L4","GTCCTAAATGCACAGCGACG"]
[15,"Reverse-R1","AAGGCGAAAGGCAAATGCATC","Amplification of overexpression right arm "]
[null,"Reverse-R2","CGAGAGTAAGGGGTCCGTCA"]
[16,"Reverse-R3","CAGACCGATAAAACACATGCGT","Nested amplification of overexpression right arm "]
[null,"Reverse-R4","TCCGGTATCTCGCGTTTGTT"]
[17,"OBP1-DF","GTCGTGACGCACAATATCCTCC","OBP1 gene quantification"]
[null,"OBP1-DR","GAGAATCTCCTCGTCGCTGAAT"]
[18,"OF-1F","CTCGCCGAAGTTGAAATGAT","Validation of Off-target site 1"]
[null,"OF-1R","TCGTCTGGTAGAGCCGAAGGT"]
[19,"OF-2F","GAATTGGCTTCATGGCTTCG","Validation of Off-target site 2"]
[null,"OF-2R","GCATTGACCAGTGTCGGAGT"]

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