LOCUS       Exported               13015 bp ds-DNA     circular SYN 05-FEB-2021
DEFINITION  Vector expressing soluble Syncytin-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Stafl K, Travnicek M, Kucerova D, Pecnova L, Krchlikova V, Galikova 
            E, Hejnar J and Trejbalova K
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 5, 2021 from SnapGene 5.2.4
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="recombinant plasmid"
                     /lab_host="Gallus gallus"
                     /mol_type="other DNA"
     repeat_region   1..21
                     /label=R region
                     /note="R region"
     repeat_region   22..101
                     /label=U5
                     /note="U5"
     primer_bind     102..119
                     /label=PBS
                     /note="PBS"
     CDS             372..2483
                     /codon_start=1
                     /label=gag
                     /note="gag"
                     /translation="MEAVIKVISSACKTYCGKISPSKKEIGAMLSLLQKEGLLMSPSDL
                     YSPGSWDPITAALSQRAMVLGKSGELKTWGLVLGALKAAREEQVTSEQAKFWLGLGGGR
                     VSPPGPECIEKPATERRIDKGEEVGETTVQRDAKMAPEEAATPKTVGTSCYHCGTAVGC
                     NCATATASAPPPPYVGSGLYPSLAGVGEQQGQGDNTSRGAEQPREEPGHAGQAPGPALT
                     DWARVREELASTGPPVVAMPVVIKTEGPAWTPLEPKLITRLADTVRTKGLRSPITMAEV
                     EALMSSPLLPHDVTNLMRVILGPAPYALWMDAWGVQLQTVIAAATRDPRHPANGQGRGE
                     RTNLDRLKGLADGMVGNPQGQAALLRPGELVAITASALQAFREVARLAEPAGPWADITQ
                     GPSESFVDFANRLIKAVEGSDLPPSARAPVIIDCFRQKSQPDIQQLIRAAPSTLTTPGE
                     IIKYVLDRQKIAPLTDQGIAAAMSSAIQPLVMAVVNRERDGQTGSGGRARGLCYTCGSP
                     GHYQAQCPKKRKSGNSRERCQLCDGMGHNAKQCRKRDGNQGQRPGRGLSSGPWPGPEQP
                     AVSLAMTMEHKDRPLVRVILTNTGSHPVKQRSVYITALLDSGADITIISEEDWPTDWPV
                     VDTANPQIHGIGGGIPMRKSRDMIEVGVINRDGSLERPLLLFPAVAMVRGSILGRDCLQ
                     GLGLRLTNL"
     gene            372..902
                     /label=p19
                     /note="p19"
     misc_feature    390..391
                     /label=splice site
     gene            909..1094
                     /label=p10
                     /note="p10"
     gene            1095..1814
                     /label=p27
                     /note="p27"
     gene            1842..2108
                     /label=p12
                     /note="p12"
     gene            2109..2480
                     /label=p15
                     /note="p15"
     CDS             2480..5188
                     /codon_start=1
                     /label=pol
                     /translation="IGRATVLTVALHLAIPLKWKSDRTPVWIDQWPLPEGKLVALTQLV
                     EKELQLGHIEPSLSCWNTPVFVIRKASGSYRLLHDLRAVNAKLVPFGAVQQGAPVLSAL
                     PRGWPLMVLDLKDCFFSIPLAEQDREAFAFTLPSVNNQAPARRFQWKVLPQGMTCSPTI
                     CQLVVGQVLEPLRLKHPALRMLHYMDDLLLAASSHDGLEAAGKEVIGTLERAGFTISPD
                     KIQREPGVQYLGYKLGSTYVAPVGLVAEPRIATLWDVQKLVGSLQWLRPALGIPPRLMG
                     PFYEQLRGSDPNEAREWNLDMKMAWREIVQLSTTAALERWDPAQPLEGAVARCEQGAIG
                     VLGQGLSTHPRPCLWLFSTQPTKAFTAWLEVLTLLITKLRASAVRTFGKEVDILLLPAC
                     FREDLPLPEGILLALRGFAGKIRSSDTPSIFDIARPLHVSLKVRVTDHPVPGPTVFTDA
                     SSSTHKGVVVWREGPRWEIKEIVDLGASVQQLEARAVAMALLLWPTTPTNVVTDSAFVA
                     KMLLKMGQEGVPSTAAAFILEDALSQRSAMAAVLHVRSHSEVPGFFTEGNDVADSQATF
                     QAYPLREAKDLHTALHIGPRALSKACNISMQQAREVVQTCPHCNSAPALEAGVNPRGLG
                     PLQIWQTDFTLEPRMAPRSWLAVTVDTASSAIVVTQHGRVTSVAAQHHWATAIAVLGRP
                     KAIKTDNGSCFTSRSTREWLARWGIAHTTGIPGNSQGQAMVERANRLLKDKIRVLAEGD
                     GFMKRIPASKQGELLAKAMYALNHFERGENTKTPVQKHWRPTVLTEGPPVKIRIETGEW
                     EKGWNVLVWGRGYAAVKNRDTDKVIWVPSRKVKPDVTQKDEVTKKDEASPLFAGISDWI
                     PWEDEQEGFQGETASNKQERPGEDTLAANES"
     CDS             2624..3189
                     /label=reverse transcriptase
     CDS             3821..4216
                     /label=RNase H
     CDS             4373..4864
                     /label=Integrase
     CDS             5076..5243
                     /codon_start=1
                     /label=env-sigPE(A)
                     /translation="AFLTGYPGKTSKKDSKEKPLATSKKDPEKTPLLPTRVNYILIIGV
                     LVLCEVTGVRA"
     CDS             5244..6899
                     /codon_start=1
                     /label=env B
                     /translation="DVHLLEQPGNLWITWANRTGQTDFCLSTQSATSPFQTCLIGIPSP
                     ISEGDFKGYVSDNCTTLEPHRLVSRGIPGGPENSTTLTYQKVSCLLLKLNVSLLDEPSE
                     LQLLGSQSLPNITNITRIPSVAGGCIGFTPYDSPAGVYGWDRREVTHILLTDPGNNPFF
                     DKASNSSKPFTVVTADRHNLFMGSEYCGAYGYRFWEMYNCSQMRQNWSICQDVWGRGPP
                     ENWCTSTGGTWVNQSKEFNETAPFSFTVNCTGSNLGNVSGCCGEPITILPPEAWVDSTQ
                     GSFTKPKALPPAIFLICGDRAWQGIPSRPVGGPCYLGKLTMLAPKHTDILKVLVNSSRT
                     GIRRKRSTSHLDDTCSDEVQLWGPTARIFASILAPGVAAAQALREIERLACWSVKQANL
                     TTSLLGDLLDDVTSIRHAVLQNRAAIDFLLLAHGHGCEDVAGMCCFNLSDQSESIQKKF
                     QLMKEHVNKIGVDSDLIGSWLRGLFGGIGEWAVHLLKGLLLGLVVILLLVVCLPCLLQM
                     LCGNRRKMINNSISYHTEYKKLQKACGQPESRIV"
     CDS             7099..8388
                     /codon_start=1
                     /label=fusion protein sigPE-SUW-TEV-rIgG
                     /translation="MEAVIKAFLTGYPGKTSKKDSKEKPLATSKKDPEKTPLLPTRVNY
                     ILIIGVLVLCEVTGVRAPPCRCMTSSSPYQEFLWRMQRPGNIDAPSYRSLSKGTPTFTA
                     HTHMPRNCYHSATLCMHANTHYWTGKMINPSCPGGLGVTVCWTYFTQTGMSDGGGVQDQ
                     AREKHVKEVISQLTRVHGTSSPYKGLDLSGENLYFQGSAPSTCSKPTCPPPELLGGPSV
                     FIFPPKPKDTLMISRTPEVTCVVVDVSQDDPEVQFTWYINNEQVRTARPPLREQQFNST
                     IRVVSTLPIAHQDWLRGKEFKCKVHNKALPAPIEKTISKARGQPLEPKVYTMGPPREEL
                     SSRSVSLTCMINGFYPSDISVEWEKNGKAEDNYKTTPAVLDSDGSYFLYSKLSVPTSEW
                     QRGDVFTCSVMHEALHNHYTQKSISRSPGK"
     sig_peptide     7099..7284
                     /label=sig.pe A
     gene            7285..7674
                     /product="first 390 bp (130 AA) from SU(W) = receptor 
                     binding domain, up to the first Ser after RBD"
                     /label=RBD from SU(W)
                     /note="up to the last Ser"
     misc_feature    7675..7701
                     /label=TEV protease recognition seq.
     CDS             7678..7698
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     gene            7702..8385
                     /label=rIgG
     repeat_region   8555..8781
                     /label=U3
                     /note="U3"
     repeat_region   8782..8802
                     /label=R region
                     /note="R region"
     repeat_region   8803..8882
                     /label=U5
                     /note="U5"
     primer_bind     8883..8900
                     /label=PBS
                     /note="PBS"
     protein_bind    9416..9432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9440..9470)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     promoter        9599..9703
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9704..10564
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10735..11323
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    11509..11649
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(11751..11942)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     repeat_region   12789..13015
                     /label=U3
                     /note="U3"
ORIGIN
        1 gccatttgac cattcaccac attggtgtgc acctgggttg atggccggac cgttgattcc
       61 ctgacgacta cgagcacctg catgaagcag aaggcttcat ttggtgaccc cgacgtgata
      121 gttagggaat agtggtcggc cacagacggc gtggcgatcc tgtctccatc cgtctcgtct
      181 atcgggaggc gagttcgatg accctggtgg agggggctgc ggcttaggga ggcagaagct
      241 gagtaccgtc ggagggagct ccagggcccg gagcgactga cccctgccga gaactcagag
      301 ggtcgtcgga agacggagag tgagcccgac gaccacccca ggcacgtctt tggtcggcct
      361 gcggatcaag catggaagcc gtcattaagg tgatttcgtc cgcgtgtaaa acctattgcg
      421 ggaaaatctc tccttctaag aaggaaatag gggccatgtt gtccctgtta caaaaggaag
      481 ggttgcttat gtctccctca gatttatatt ctccggggtc ctgggatccc atcactgcgg
      541 cgctctccca gcgggcaatg gtacttggaa aatcgggaga gttaaaaacc tggggattgg
      601 ttttgggggc attgaaggcg gctcgagagg aacaggttac atctgagcaa gcaaagtttt
      661 ggttgggatt agggggaggg agggtctctc ccccaggtcc ggagtgcatc gagaaaccag
      721 ctacggagcg gcgaatcgac aaaggggagg aggtgggaga aacaactgtg cagcgagatg
      781 cgaagatggc gccagaggaa gcggccacac ctaaaaccgt tggcacatcc tgctatcatt
      841 gcggaacagc tgttggctgc aattgcgcca ccgccacagc ctcggcccct cctccccctt
      901 atgtggggag tggtttgtat ccttccctgg cgggggtggg agagcagcag ggccagggag
      961 ataacacgtc tcggggggcg gagcagccaa gggaggagcc agggcacgcg ggtcaggccc
     1021 ctgggccggc cctgactgac tgggcaaggg taagggagga gcttgcgagt actggtccgc
     1081 ccgtggtggc catgcctgta gtgattaaga cagagggacc cgcctggacc cctctggagc
     1141 caaaattgat cacaagactg gctgatacgg tcaggaccaa gggcttacga tccccgatca
     1201 ctatggcaga agtggaagcg ctcatgtcct ccccgttgct gccgcatgac gtcacgaatc
     1261 taatgagagt gattttagga cctgccccat atgccttatg gatggacgct tggggagtcc
     1321 aactccagac ggttatagcg gcagccactc gcgacccccg acacccagcg aacggtcaag
     1381 ggcgggggga acggactaac ttggatcgat taaagggctt agctgatggg atggtgggca
     1441 acccacaggg tcaggccgca ttattaagac cgggggaatt ggttgctatt acggcgtcgg
     1501 ctctccaggc gtttagagaa gttgcccggc tggcggaacc tgcaggtcca tgggcggaca
     1561 tcacgcaggg accatctgag tcctttgttg attttgccaa tcggcttata aaggcggttg
     1621 aggggtcaga tctcccgcct tccgcgcggg ctccggtgat cattgactgc tttaggcaga
     1681 agtcacagcc agatattcag cagcttatac gggcagcacc ctccacgctg accaccccag
     1741 gagagataat caaatatgtg ctagacaggc agaagattgc ccctcttacg gatcaaggca
     1801 tagccgcggc catgtcgtct gctatccagc ccttagttat ggcagtagtc aatagagaga
     1861 gggatggaca aactgggtcg ggtggtcgtg cccgagggct ctgctacact tgtggatccc
     1921 cgggacatta tcaggcacag tgcccgaaaa aacgaaagtc aggaaacagc cgtgagcgat
     1981 gtcagctgtg tgacgggatg ggacacaacg ctaaacagtg taggaagcgg gatggcaacc
     2041 agggccaacg cccaggaaga ggtctctctt cggggccgtg gcccggccct gagcagcctg
     2101 ccgtctcgtt agcgatgaca atggaacata aagatcgccc cttggttagg gtcattctga
     2161 ctaacactgg gagtcatcca gtcaaacaac gttcggtgta tatcaccgcg ctgttggact
     2221 ccggagcgga catcactatt atttcggagg aggattggcc tactgattgg ccggtggtgg
     2281 acaccgcgaa cccacagatc catggcatag gagggggaat tcccatgcga aaatcccggg
     2341 atatgataga ggtgggggtt attaaccgag acgggtcgtt ggagcgaccc ctgctcctct
     2401 tccccgcagt cgctatggtt agagggagta tcctaggaag agattgtctg cagggcctag
     2461 ggctccgctt gacaaattta tagggagggc cactgttctt actgttgcgc tacatctggc
     2521 tattccgctc aaatggaagt cagaccgcac gcctgtgtgg attgaccagt ggccccttcc
     2581 tgaaggtaaa cttgtagcgc taacgcaatt agtggaaaaa gaattacagt taggacatat
     2641 agagccctca cttagttgtt ggaacacacc tgtttttgtg atccggaagg cttccgggtc
     2701 ttatcgctta ttgcatgatt tgcgcgctgt taacgccaag cttgtccctt ttggggccgt
     2761 ccaacagggg gcgccagttc tctccgcgct cccgcgtggc tggcccctga tggtcctaga
     2821 cctcaaggat tgcttctttt ctatccctct tgcggaacaa gatcgcgaag cttttgcatt
     2881 tacgctcccc tctgtgaata accaggcccc cgctcgaaga ttccaatgga aggtcttgcc
     2941 ccaagggatg acctgttctc ccactatctg tcagttggta gtgggtcagg tgctcgagcc
     3001 cttgcgactc aagcacccag ctctgcgcat gttgcattat atggacgatc ttttgctagc
     3061 cgcctcaagt catgatgggt tggaagcggc agggaaggag gttatcggta cattggaaag
     3121 agccgggttc actatttcgc cggataagat ccagagggag cccggagtac aatatcttgg
     3181 gtacaagtta ggcagtacgt atgtagcacc cgtaggcttg gtagcagaac ccaggatagc
     3241 caccttgtgg gatgttcaaa agctggtggg gtcacttcag tggcttcgcc cagcgttagg
     3301 gatcccgcca cgactgatgg gtccctttta tgagcagtta cgagggtcag atcctaacga
     3361 ggcgagggaa tggaatctag acatgaaaat ggcctggaga gagatcgtac agcttagcac
     3421 tactgctgcc ttggaacgat gggaccctgc ccagcctctg gaaggagcgg tcgctagatg
     3481 tgaacagggg gcaatagggg tcctgggaca gggactgtcc acacacccaa ggccatgttt
     3541 gtggttattc tccacccaac ccaccaaggc gtttactgct tggttagaag tgctcaccct
     3601 tttgattact aagctacgcg cttcggcagt gcgaaccttt ggcaaggagg ttgatatcct
     3661 cctgttgcct gcatgcttcc gggaggacct tccgctcccg gaggggatcc tgttagcact
     3721 tagggggttt gcaggaaaaa tcaggagtag tgacacgcca tctatttttg acattgcgcg
     3781 tccactgcat gtttctctga aagtgagggt taccgaccac cctgtgccgg gacccactgt
     3841 ctttaccgac gcctcctcaa gcacccataa aggggtggta gtctggaggg agggcccaag
     3901 gtgggagata aaagaaatag ttgatttggg ggcaagtgta caacaactgg aggcacgcgc
     3961 tgtggccatg gcacttctgc tgtggccgac aacgcccact aatgtagtga ctgactctgc
     4021 gtttgttgcg aaaatgttac tcaagatggg acaggaggga gtcccgtcta cagcggcagc
     4081 ttttatttta gaggatgcgt taagccaaag gtcagccatg gccgccgttc tccacgtgcg
     4141 gagtcattct gaagtgccag ggtttttcac agaaggaaat gacgtggcag atagccaagc
     4201 cacctttcaa gcgtatccct tgagagaggc taaagatctt cataccgctc tccatattgg
     4261 accccgcgcg ctatccaaag cgtgtaatat atctatgcag caggctaggg aggttgttca
     4321 gacctgcccg cattgtaatt cagcccctgc gttggaggcc ggggtaaacc ctaggggttt
     4381 gggaccccta cagatatggc agacagactt tacgcttgag cctagaatgg ctccccgttc
     4441 ctggctcgct gttactgtgg acaccgcctc atcagcgata gtcgtaactc agcatggccg
     4501 tgttacatcg gttgctgcac aacatcattg ggccacggct atcgccgttt tgggaagacc
     4561 aaaggccata aaaacagata acgggtcctg cttcacgtct agatccacgc gggagtggct
     4621 cgcgagatgg gggatagcac acaccaccgg gattccggga aattcccagg gtcaagctat
     4681 ggtagagcgg gccaaccggc tcctgaaaga taagatccgt gtgcttgcgg agggggacgg
     4741 ctttatgaaa agaatccccg ccagcaaaca gggggaacta ctagccaagg caatgtatgc
     4801 cctcaatcac tttgagcgtg gtgaaaacac aaaaacaccg gtacaaaaac actggagacc
     4861 taccgttctt acagaaggac ccccggttaa aatacgaata gagacagggg agtgggaaaa
     4921 aggatggaac gtgctggtct ggggacgagg ttatgccgct gtgaaaaaca gggacactga
     4981 taaggttatt tgggtaccct ctcgaaaggt taaaccggac gtcacccaaa aggatgaggt
     5041 gactaagaaa gatgaggcga gccctctttt tgcaggcatt tctgactgga taccctggga
     5101 agacgagcaa gaaggattcc aaggagaaac cgctagcaac aagcaagaaa gacccggaga
     5161 agacaccctt gctgccaacg agagttaatt atattctcat tattggtgtc ctggtcttgt
     5221 gtgaggttac gggggtaaga gctgatgtcc acttactcga gcagccaggg aacctttgga
     5281 ttacatgggc caaccgtaca ggccaaacgg atttctgcct ctctacacag tcagccacct
     5341 ccccttttca aacatgtttg ataggtatcc catcccctat ttccgaaggt gattttaagg
     5401 gatatgtctc tgataattgc accactttgg aacctcaccg gttagtctcg agaggcattc
     5461 ctggcggccc tgagaacagc acaaccctca cctatcagaa ggtttcatgc ttgttgttaa
     5521 agctgaatgt ttctctgttg gacgagccat cagaactaca actgctaggt tcccagtctc
     5581 tccccaatat aactaatatt actcggatcc ccagtgtggc tggaggatgc ataggcttta
     5641 ccccatacga tagtccggct ggtgtctacg gatgggaccg gagagaggtt acacacatcc
     5701 ttctgaccga cccagggaac aatcctttct ttgataaggc ctctaactcc tcgaaaccgt
     5761 ttacagtagt gacagcggac aggcacaatc tctttatggg gagtgagtac tgtggtgcat
     5821 atggctacag gttctgggaa atgtacaatt gctcccaaat gagacagaat tggtccatct
     5881 gtcaggatgt gtggggccga ggcccccccg aaaattggtg cacaagcaca ggaggtacat
     5941 gggttaatca atcaaaggaa tttaatgaga cagcgccatt cagttttact gtgaactgta
     6001 ctggcagtaa tttgggtaat gtcagcggat gttgcggaga accaatcacg attctcccac
     6061 cagaggcgtg ggtcgacagc acacaaggta gtttcaccaa accaaaagcg ctaccacccg
     6121 caattttcct catttgtggg gatcgcgcat ggcaaggaat tcccagtcgt ccggtagggg
     6181 gcccctgcta tttaggcaag cttaccatgt tagcacctaa gcatacagat attctcaagg
     6241 tgcttgtcaa ttcatcgcgg acaggtataa gacgtaaacg aagcacctca cacctggatg
     6301 atacatgctc agatgaagtg cagctttggg gtcctacagc aagaatcttt gcatctatcc
     6361 tagccccggg ggtagcagct gcgcaagcct taagagaaat tgagagacta gcctgttggt
     6421 ccgttaaaca ggctaacttg acaacatcac tcctcgggga cttattggat gatgtcacga
     6481 gtattcgaca cgcggtcctg cagaaccgag cggctattga cttcttgctc ctagctcacg
     6541 gccatggctg tgaggacgtt gccggaatgt gctgtttcaa tttgagtgat cagagtgagt
     6601 ctatacagaa gaagttccag ctaatgaagg aacatgtcaa taagatcggc gtggatagcg
     6661 acctaattgg aagttggctg cgaggactat tcgggggaat aggagaatgg gccgttcatt
     6721 tgctgaaagg actgcttttg gggcttgtag ttattttgtt gctagtagtg tgcctgcctt
     6781 gccttttgca aatgttatgc ggtaatagga gaaagatgat taataactcc atcagctacc
     6841 acacggaata taagaagctg caaaaggcct gtgggcagcc tgaaagcaga atagtataag
     6901 gcagtacatg ggtggtggta tagcgcttgc gagtccatcg agcaaggcag gaaagacagc
     6961 tattggtaat tgtgaaatac gcttttgtct gtgtgctgca ggagctgagc tgactctgct
     7021 ggtggcctcg cgtaccactg tggcatcgat tctagaccac tgtggccagg cggtagctgg
     7081 gacgtgcagc cgaccaccat ggaagccgtc attaaggcat ttctgactgg ataccctggg
     7141 aagacgagca agaaggactc caaggagaaa ccgctagcaa caagcaagaa agacccggag
     7201 aagacaccct tgctgccaac gagagttaat tatattctca ttattggtgt cctggtcttg
     7261 tgtgaggtta cgggggtaag agctcctcca tgccgctgta tgaccagtag ctccccttac
     7321 caagagtttc tatggagaat gcagcgtccc ggaaatattg atgccccatc gtataggagt
     7381 ctttctaagg gaacccccac cttcactgcc cacacccata tgccccgcaa ctgctatcac
     7441 tctgccactc tttgcatgca tgcaaatact cattattgga caggaaaaat gattaatcct
     7501 agttgtcctg gaggacttgg agtcactgtc tgttggactt acttcaccca aactggtatg
     7561 tctgatgggg gtggagttca agatcaggca agagaaaaac atgtaaaaga agtaatctcc
     7621 caactcaccc gggtacatgg cacctctagc ccctacaaag gactagatct ctccggagag
     7681 aatctttatt ttcagggctc cgcaccctcg acatgcagca agcccacgtg cccaccccct
     7741 gaactcctgg ggggaccgtc tgtcttcatc ttccccccaa aacccaagga caccctcatg
     7801 atctcacgca cccccgaggt cacatgcgtg gtggtggacg tgagccagga tgaccccgag
     7861 gtgcagttca catggtacat aaacaacgag caggtgcgca ccgcccggcc gccgctacgg
     7921 gagcagcagt tcaacagcac gatccgcgtg gtcagcaccc tccccatcgc gcaccaggac
     7981 tggctgaggg gcaaggagtt caagtgcaaa gtccacaaca aggcactccc ggcccccatc
     8041 gagaaaacca tctccaaagc cagagggcag cccctggagc cgaaggtcta caccatgggc
     8101 cctccccggg aggagctgag cagcaggtcg gtcagcctga cctgcatgat caacggcttc
     8161 tacccttccg acatctcggt ggagtgggag aagaacggga aggcagagga caactacaag
     8221 accacgccgg ccgtgctgga cagcgacggc tcctacttcc tctacagcaa gctctcagtg
     8281 cccacgagtg agtggcagcg gggcgacgtc ttcacctgct ccgtgatgca cgaggccttg
     8341 cacaaccact acacgcagaa gtccatctcc cgctctccgg gtaaatgagt taacacgcgt
     8401 ctgcagccaa gcttatcgat gcgatgtacg ggccagatat acgcgtatct gaggggacta
     8461 gggtgtgttt aggcgaaaag cggggcttcg gttgtacgcg gttaggagtc cccttaggat
     8521 atagtagttt cgcttttgca tagggagggg gaaatgtagt cttatgcaat actcttgtag
     8581 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     8641 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     8701 ggtctgacat ggattggacg aaccactgaa ttccgcattg cagagatatt gtatttaagt
     8761 gcctagctcg atacaataaa cgccatttga ccattcacca cattggtgtg cacctgggtt
     8821 gatggccgga ccgttgattc cctgacgact acgagcacct gcatgaagca gaaggcttca
     8881 tttggtgacc ccgacgtgat agttagggaa tagtggtcgg ccacagacgg cgtggcgatc
     8941 ctgtctccat ccgtctcgtc tatcgggagg cgagttcgat gaccctggtg gagggggctg
     9001 cggcttaggg aggcagaagc tgagtaccgt cggaggggat ccacaggacg ggtgtggtcg
     9061 ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc
     9121 caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata
     9181 gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga
     9241 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga
     9301 ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta
     9361 actgtgataa actaccgcat taaagcttcc aaacttggct gtttcctgtg tgaaattgtt
     9421 atccgctcac aattccacac attatacgag ccggaagcat aaagtgtaaa acctggggtg
     9481 cctaatgagt gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     9541 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     9601 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     9661 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     9721 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     9781 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     9841 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     9901 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     9961 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    10021 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    10081 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    10141 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    10201 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac
    10261 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    10321 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    10381 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10441 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10501 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10561 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10621 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    10681 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    10741 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10801 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10861 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
    10921 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10981 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    11041 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    11101 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    11161 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    11221 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    11281 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    11341 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    11401 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    11461 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
    11521 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
    11581 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
    11641 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
    11701 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
    11761 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
    11821 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
    11881 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
    11941 acttgatgcc tccgtgtaag ggggaatttc tgttcatggg ggtaatgata ccgatgaaac
    12001 gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt
    12061 gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc
    12121 aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg
    12181 cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg
    12241 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc
    12301 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc
    12361 gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc
    12421 caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt
    12481 tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
    12541 ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg
    12601 gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgatgcgatg
    12661 tacgggccag atatacgcgt atctgagggg actagggtgt gtttaggcga aaagcggggc
    12721 ttcggttgta cgcggttagg agtcccctta ggatatagta gtttcgcttt tgcataggga
    12781 gggggaaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
    12841 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
    12901 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
    12961 tgaattccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaac
//
