LOCUS       Exported               12557 bp ds-DNA     circular SYN 03-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 3, 2022 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12557
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     CDS             complement(103..963)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 103 .. 894 / #ccffcc
                        2: 895 .. 963 / #ccffcc / signal sequence
                     Cleavage site after base 894"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(964..1068)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      1149..4200
                     /label=AMA1/2
                     /label=AMA1
                     /note="color: #ffff00"
     promoter        4254..5139
                     /label=Ptef (nid)
                     /note="color: #ffffff"
     CDS             5140..9243
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /note="color: #993366"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     sig_peptide     9244..9267
                     /label=NLS
                     /note="color: #cc99b2"
     CDS             9247..9267
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    9271..9659
                     /label=T.bd.gliN_gliA_Afum
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    9660..9673
                     /label=Eco72I/SmiI site-Marker insertion
                     /note="color: #a6acb3"
     misc_feature    complement(9674..10170)
                     /label=P.gpdA(Anid)
                     /note="color: #008000; direction: LEFT"
     misc_feature    10171..11015
                     /label=gpdA promoter Afum
                     /note="color: #99cc00; direction: RIGHT"
     misc_feature    11016..11021
                     /label=Bsp1407I R-site for sgRNA insertion
                     /note="color: #a6acb3"
     misc_feature    11022..11169
                     /label=sgRNA-scaffold
                     /note="Nodvig 2015 pFC334"
                     /note="color: #ff00ff"
     misc_feature    11170..11669
                     /label=T.tef1_Afum
                     /note="color: #ff0000"
     rep_origin      complement(11901..12489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
       61 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      241 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1081 aaaagtgcca cctgacgcca gggttttccc agtcacgacg gacggccagt gccaagctta
     1141 acgcgtaccc gggcccagta tatgttccgc agatgactgg agctctgcca tacgtgccct
     1201 ctcaagcacc atttgttcca tctacagaga ctagtcacca actagtctat caagactcac
     1261 agggtacatt gctgagacca actgaccaga ggcagggtag cggattgacg gctccatctc
     1321 cttcacttac aaggtctatt gaaagccctt tagcatcacc aagcggagaa tagattgtta
     1381 agcttatttt ttgtatactg ttttgtgata gcacgaagtt tttccacggt atcttgtaaa
     1441 aatatatatt tgtggcgggc ttacctacat caaattaata agagactaat tataaactaa
     1501 acacacaagc aagctacttt agggtaaaag tttataaatg cttttgacgt ataaacgttg
     1561 cttgtattta ttattacaat taaaggtgga tagaaaacct agagactagt tagaaactaa
     1621 tctcaggttt gcgttaaact aaatcagagc ccgagaggtt aacagaacct agaaggggac
     1681 tagatatccg ggtagggaaa caaaaaaaaa aaacaagaca gccacatatt agggagacta
     1741 gttagaagct agttccagga ctaggaaaat aaaagacaat gataccacag tctagttgac
     1801 aactagatag attctagatt gaggccaaag tctctgagat ccaggttagt tgcaactaat
     1861 actagttagt atctagtctc ctataactct gaagctagaa taacttacta ctattatcct
     1921 caccactgtt cagctgcgca aacggagtga ttgcaaggtg ttcagagact agttattgac
     1981 tagtcagtga ctagcaataa ctaacaaggt attaacctac catgtctgcc atcaccctgc
     2041 acttcctcgg gctcagcagc cttttcctcc tcattttcat gctcattttc cttgtttaag
     2101 actgtgacta gtcaaagact agtccagaac cacaaaggag aaatgtctta ccactttctt
     2161 cattgcttgt ctcttttgca ttatccatgt ctgcaactag ttagagtcta gttagtgact
     2221 agtccgacga ggacttgctt gtctccggat tgttggagga actctccagg gcctcaagat
     2281 ccacaacaga gccttctaga agactggtca ataactagtt ggtctttgtc tgagtctgac
     2341 ttacgaggtt gcatactcgc tccctttgcc tcgtcaatcg atgagaaaaa gcgccaaaac
     2401 tcgcaatatg gctttgaacc acacggtgct gagactagtt agaatctagt cccaaactag
     2461 cttggatagc ttacctttgc cctttgcgtt gcgacaggtc ttgcagggta tggttccttt
     2521 ctcaccagct gatttagctg ccttgctacc ctcacggcgg atctgccata aagagtggct
     2581 agaggttata aattagcact gatcctaggt acggggctga atgtaacttg cctttccttt
     2641 ctcatcgcgc ggcaagacag gcttgctcaa attcctacca gtcacagggg tatgcacggc
     2701 gtacggacca cttgaactag tcacagatta gttagcaact agtctgcatt gaatggctgt
     2761 acttacgggc cctcgccatt gtcctgatca tttccagctt caccctcgtt gctgcaaagt
     2821 agttagtgac tagtcaagga ctagttgaaa tgggagaaga aactcacgaa ttctcgactc
     2881 ccttagtatt gtggtccttg gacttggtgc tgctatatat tagctaatac actagttaga
     2941 ctcacagaaa cttacgcagc tcgcttgcgc ttcttggtag gagtcggggt tgggagaaca
     3001 gtgccttcaa acaagccttc ataccatgct acttgactag tcagggacta gtcaccaagt
     3061 aatctagata ggacttgcct ttggcctcca tcagttcctt catagtggga ggaccattgt
     3121 gcaatgtaaa ctccatgccg tgggagttct tgtccttcaa gtgcttgacc aatatgtttc
     3181 tgttggcaga gggaacctgt caactagtta ataactagtc agaaactatg atagcagtag
     3241 actcactgta cgcttgaggc atcccttcac tcggcagtag acttcatatg gatggatatc
     3301 aggcacgcca ttgtcgtcct gtggactagt cagtaactag gcttaaagct agtcgggtcg
     3361 gcttactatc ttgaaatccg gcagcgtaag ctccccgtcc ttaactgcct cgagatagtg
     3421 acagtactct ggggactttc ggagatcgtt atcgttatcg cgaatgctcg gcatactaac
     3481 tgttgactag tcttggacta gtcccgagca aaaaggattg gaggaggagg aggaaggtga
     3541 gagtgagaca aagagcgaaa taagagcttc aaaggctatc tctaagcagt atgaaggtta
     3601 agtatctagt tcttgactag atttaaagag atttcgacta gttatgtacc tggagtttgg
     3661 atataggaat gtgttgtggt aacgaaatgt aagggggagg aaagaaaaag tcgtcaagag
     3721 gtaactctaa gtcggccatt cctttttggg aggcgctaac cataaacggc atggtcgact
     3781 tagagttagc tcagggaatt tagggagtta tctgcgacca ccgaggaacg gcggaatgcc
     3841 aaagaatccc gatggagctc tagctggcgg ttgacaaccc caccttttgg cgtttctgcg
     3901 gcgttgcagg cgggactgga tacttcgtag aaccagaaag gcaaggcaga acgcgctcag
     3961 caagagtgtt ggaagtgata gcatgatgtg ccttgttaac taggtaccaa tctgcagtat
     4021 gcttgatgtt atccaaagtg tgagagagga aggtccaaac atacacgatt gggagagggc
     4081 ctaggtataa gagtttttga gtagaacgca tgtgagccca gccatctcga ggagattaaa
     4141 cacgggccgg catttgatgg ctatgttagt accccaatgg aaacggtgag agtccagtgg
     4201 gaagatctca tggtcatagc tgtttccgct gagggtttaa ttaagacctc agccgagaca
     4261 gcagaatcac cgcccaagtt aagcctttgt gctgatcatg ctctcgaacg ggccaagttc
     4321 gggaaaagca aaggagcgtt tagtgagggg caatttgact cacctcccag gcaacagatg
     4381 aggggggcaa aaagaaagaa attttcgtga gtcaatatgg attccgagca tcattttctt
     4441 gcggtctatc ttgctacgta tgttgatctt gacgctgtgg atcaagcaac gccactcgct
     4501 cgctccatcg caggctggtc gcagacaaat taaaaggcgg caaactcgta cagccgcggg
     4561 gttgtccgct gcaaagtaca gagtgataaa agccgccatg cgaccatcaa cgcgttgatg
     4621 cccagctttt tcgatccgag aatccaccgt agaggcgata gcaagtaaag aaaagctaaa
     4681 caaaaaaaaa tttctgcccc taagccatga aaacgagatg gggtggagca gaaccaagga
     4741 aagagtcgcg ctgggctgcc gttccggaag gtgttgtaaa ggctcgacgc ccaaggtggg
     4801 agtctaggag aagaatttgc atcgggagtg gggcgggtta cccctccata tccaatgaca
     4861 gatatctacc agccaagggt ttgagcccgc ccgcttagtc gtcgtcctcg cttgcccctc
     4921 cataaaagga tttcccctcc ccctcccaca aaattttctt tcccttcctc tccttgtccg
     4981 cttcagtacg tatatcttcc cttccctcgc ttctctcctc catccttctt tcatccatct
     5041 cctgctaact tctctgctca gcacctctac gcattactag ccgtagtatc tgagcacttc
     5101 tcccttttat attccacaaa acataacaca accttcacca tggacaagaa gtatagcatc
     5161 gggctggaca ttggaacgaa ctcggttggt tgggctgtga ttacggacga atacaaggtg
     5221 ccatccaaga agtttaaggt cctgggaaac accgaccgtc actcaatcaa gaagaatctc
     5281 attggagccc tgctcttcga tagtggggag accgccgaag ctactcgact gaagcgaacg
     5341 gctcgccggc gttatacacg acgcaagaat cgcatctgct acctccagga gattttcagc
     5401 aacgaaatgg ctaaggttga tgactcattc tttcatcgac tcgaagaaag tttcttggtc
     5461 gaggaggata agaagcacga gcgccatccg atctttggta acattgtgga tgaggttgcc
     5521 tatcacgaaa agtacccaac tatctatcat cttcgtaaga agctggtcga tagcacggac
     5581 aaggctgatt tgcgacttat ctacctggca ctcgcgcaca tgattaagtt ccgcggccat
     5641 tttcttatcg agggtgacct gaaccccgat aattctgacg ttgataagct cttcatccag
     5701 ttggtccaaa cctacaatca gctgtttgag gaaaacccta ttaatgcatc tggcgtggac
     5761 gccaaggcta tcctttcggc gcgcctgtct aagtcgcggc gtttggagaa ccttatcgca
     5821 caactccccg gcgaaaagaa gaacggcctc ttcggtaatt tgattgcgtt gtcacttggt
     5881 ctgactccta acttcaagag taattttgac ctggcagagg atgcgaagct ccagttgtct
     5941 aaggatacgt atgatgacga tctcgacaac ttgcttgccc aaatcggtga ccagtacgct
     6001 gatcttttcc tggccgctaa gaatctctca gatgcaatcc tgctcagtga cattttgcgg
     6061 gtcaacaccg agattactaa ggcccccctg tcagctagta tgatcaagcg gtatgatgag
     6121 caccatcagg acctcacctt gcttaaggcc ctcgtgcgtc agcaattgcc tgagaagtat
     6181 aaggaaatct tctttgacca atccaagaac ggatacgcag ggtatattga tggcggtgcg
     6241 agccaggagg aattctacaa gtttatcaag ccgattttgg agaagatgga cggcactgag
     6301 gaactgctcg tcaagctgaa tcgcgaagat ttgcttcgta agcaacgaac gttcgacaac
     6361 ggctccatcc cgcaccagat tcatctgggc gagctccacg ccatccttcg acgccaggaa
     6421 gatttctacc catttctgaa ggacaaccgt gagaagatcg aaaagattct tacattccga
     6481 atcccctact atgtgggacc tttggcccgt gggaattccc gatttgcttg gatgacccga
     6541 aagagcgagg aaaccatcac tccgtggaac ttcgaggaag tcgtggacaa gggtgcatcc
     6601 gcgcagagct tcattgagcg gatgaccaat tttgataaga accttccgaa tgaaaaggtc
     6661 ctgccaaagc attcgctgct ctacgagtat ttcaccgtgt ataacgaact gactaaggtc
     6721 aagtacgtga cggagggaat gcggaagcca gccttcctct caggggaaca aaagaaggct
     6781 atcgtcgatt tgctttttaa gaccaatcgt aaagtgactg ttaagcagct gaaggaggat
     6841 tatttcaaga agattgaatg tttcgactcc gtcgagatca gcggcgtgga agatcgcttt
     6901 aacgcttccc tcggtaccta ccacgacctg ctcaagatca ttaaggacaa ggatttcctc
     6961 gataacgagg aaaatgagga catcttggaa gatattgtcc tcacgttgac actttttgag
     7021 gaccgcgaaa tgatcgagga acggctcaag acatatgccc atttgttcga cgataaggtg
     7081 atgaagcagc tgaagcggcg tcgatacacc ggatggggtc gccttagccg gaagctgatc
     7141 aacggcattc gagataagca atctggtaag actatcttgg atttccttaa gtcggacggc
     7201 ttcgccaacc gcaattttat gcagcttatt cacgacgatt ccctgacgtt caaggaggac
     7261 atccagaagg cacaagtctc aggacaaggg gattccctgc acgagcatat cgccaacctg
     7321 gctggatccc cggcgatcaa gaaggggatt cttcagaccg tcaaggttgt cgacgagctg
     7381 gtcaaggtga tgggccgtca taagccagaa aacatcgtga ttgagatggc ccgagaaaat
     7441 cagaccactc aaaagggtca gaagaacagc cgcgagcgga tgaagcggat cgaggaaggc
     7501 attaaggaac ttggttctca gatcctgaag gagcaccctg ttgaaaacac acagctccaa
     7561 aatgagaagc tgtatctcta ctatttgcaa aatggacgcg acatgtacgt cgatcaggag
     7621 ctcgacatta accggttgtc ggactacgat gttgaccata tcgtcccgca atccttcctt
     7681 aaggacgata gcattgataa caaggtgctg actcgctcag ataagaaccg gggcaagtcc
     7741 gacaatgttc caagcgagga agtggttaag aagatgaaga actactggcg ccaattgctt
     7801 aatgccaagc tcatcacaca gcgcaagttt gacaacttga ccaaggccga gcggggaggg
     7861 ctgagtgaac tcgataaggc tggcttcatc aagcgtcaac tcgtggagac gcgacagatc
     7921 acaaagcacg ttgctcagat tctggactcc cggatgaaca caaagtacga cgagaatgat
     7981 aagctcatcc gtgaagttaa ggtcattacc ctcaagtcta agttggtgtc ggatttccgc
     8041 aaggacttcc aattttataa ggttcgggag atcaacaatt atcaccatgc acatgatgcg
     8101 tacctcaacg cagtcgtggg aactgcgctc atcaagaagt atcccaagtt ggagtccgaa
     8161 ttcgtctacg gggattataa ggtttacgac gtccgcaaga tgatcgccaa gagtgagcag
     8221 gaaattggca aggccacggc taagtatttc ttttactcca acatcatgaa tttctttaag
     8281 acggagatca cactcgccaa tggagaaatc cgtaagcgac ctttgattga gaccaacggc
     8341 gagactggtg aaatcgtttg ggataagggg cgcgacttcg ctaccgtgcg gaaggttctg
     8401 agcatgccgc aagtcaatat cgtcaagaaa accgaggtgc agacaggcgg tttctctaag
     8461 gaatcgattc ttccaaagcg taactctgac aagctgatcg ctcgaaagaa ggattgggac
     8521 cccaagaagt atggagggtt cgattctcct acagtggcat actcggttct cgttgtcgcg
     8581 aaggttgaga agggaaagtc taagaagctg aagtcggtca aggaactgct cgggatcacc
     8641 attatggagc gctccagctt cgaaaagaat cccatcgact ttctcgaggc caagggctat
     8701 aaggaagtca agaaggatct tatcattaag ctgcctaagt actctttgtt cgagcttgaa
     8761 aacggtcgaa agcgaatgct cgcatcggca ggagagttgc agaaggggaa tgaattggca
     8821 cttccctcaa agtacgtgaa cttcctgtat ctcgcgtccc actacgagaa gctgaagggt
     8881 agccctgagg acaacgaaca gaagcaactt tttgttgagc aacacaagca ttatctggat
     8941 gagatcattg aacagatttc agagttcagt aagcgcgtca tcctcgccga tgctaatctc
     9001 gacaaggtgt tgtcggccta caacaagcac cgtgacaagc cgatccgaga gcaggctgaa
     9061 aatatcattc atctgttcac cctcactaac ttgggagcac cagcagcgtt caagtatttt
     9121 gatacgacaa tcgaccgtaa gcgatacacg tccacaaagg aggtgcttga tgcgaccctg
     9181 attcatcaat ccatcactgg gctctatgaa acccgtatcg accttagtca actggggggc
     9241 gaccctccca agaagaagcg caaggtctga agctaagctc gggatggagt gatttctaag
     9301 tagtcagagt acgataagaa tctagacatt tctacgcttg tcacaggtta tttcctgacg
     9361 cggtgtctcc attagcatgg tgtacgtccc ttgcaatata cggaagctag gaatggattc
     9421 atagcttgaa ttgaacagtc acgtcgaaca tgctggccaa tatcaactcg gaaggatgcg
     9481 tctcgacaac tatccatagc taatagagat tactgtaagc cgagagacga caggagtgga
     9541 cacagaaaca ctctccattg ccatatgctg aaagtgatac aaaagaaagc acacactgga
     9601 tacaatcgct attgacccct tcatactcga caaaaagcag acatgtttcg accagatacc
     9661 acgtgattta aatcggtgat gtctgctcaa gcggggtagc tgttagtcaa gctgcgatga
     9721 agtgggaaag ctcgaactga aaggttcaaa ggaataaggg atgggaagga tggagtatgg
     9781 atgtagcaaa gtacttactt aggggaaata aaggttcttg gatgggaaga tgaatatact
     9841 gaagatggga aaagaaagag aaaagaaaag agcagctggt ggggagagca ggaaaatatg
     9901 gcaacaaatg ttggactgac gcaacgacct tgtcaacccc gccgacacac cgggcggaca
     9961 gacggggcaa agctgcctac cagggactga gggacctcag caggtcgagt gcagagcacc
    10021 ggatgggtcg actgccagct tgtgttcccg gtctgcgccg ctggccagct cctgagcggc
    10081 ctttccggtt tcatacaccg ggcaaagcag gagaggcacg atatttggac gccctacaga
    10141 tgccggatgg gccaattagg gagcttacgc cttagtgcga tgcaactgga caaagtcctt
    10201 ccgtttctac tgcgtcttac atcatctggt atctacgcaa gccgcccact taccatatga
    10261 ataagaggca ctcaggtttt ccctcacccc cccgaagcga tggtaagcgg gtgccaaatg
    10321 catcgggagt ttctctatca taataaccta ggtattccgt aatctattac cagtctttcc
    10381 gaagagctgg tagcaactgc acgagatttg taggagcgag tacccggctg gacgagcacg
    10441 cagcacggct attggtcagc atggtagcta ccgaggggag gcaggccgcc caaatatcgt
    10501 gagtctcctg ctttgcccgg tgtatgaaac cggaaaagct gctatagagc ttctgggcgg
    10561 cgcatgtcgg gaaaccagca gcaagctgac ccagaaagac ccgtcctcaa gccattaccg
    10621 tactaatcaa ttatttgtgt agcaacactg ggaagctgta gtgcataggc tggagcagct
    10681 atttggcctt tagccccgtc tgtccgcccg gtgtgcggtt tcgactggcg cgcaagctca
    10741 aggtgatcag gtcgttgcgt cagtcggaga caacaagcca ttgccttttc tactgcccct
    10801 cccccgctgg tggccttttt ctctcatctt ctcctctctt cccatcatca gcatcattaa
    10861 tctactgtct ctctttcttt ctatcattct ataaagtaag aacatatcca tcttccctca
    10921 atcccgtcta caatagtgtc ctcttcacta ctctgtctct atctctcaaa gcttgactga
    10981 catttacccc gctcagtacc agacgaatct acacatgtac agttttagag ctagaaatag
    11041 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
    11101 tggccggcat ggtcccagcc tcctcgctgg cgccggctgg gcaacatgct tcggcatggc
    11161 gaatgggaca gggctggaga gtgaaaaatt ttgcttgaac aatcttctat gagatgtcat
    11221 gaaatgacct ttttacgatt aaaatggaat tagagtgaat tcattttcat gtctcacaaa
    11281 tctttcgaat ccgtagcgta actacattca tgtgttgtcg agtacctacc taatgtcgct
    11341 ttgaggtact agccttgtca ccctcttgta ctttggaact ctttaaatta ccttcatcct
    11401 aaggcattca atgctccctg ttgtggaagg ctctttcatt tcaagcacca gattctgcaa
    11461 gacaacgttc atctccttta agttatgtta atgtaactac ctcgaccatc aatgaccaat
    11521 gcacagagaa ctcttgtctc ttctatatcc ttgctctcta gttgaaatgg tcgactttaa
    11581 ttgatctaca attagcgaac tacgactttc cgaagaaact acggcgaacg cttcacgcaa
    11641 tgctatgaca gacattgaag ataacaatgt taatgaatcg gccaacgcgc ggggagaggc
    11701 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    11761 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    11821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    11881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    11941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    12001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    12061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    12121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    12181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    12241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    12301 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    12361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    12421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    12481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    12541 tcacgttaag ggatttt
//
