Extended Data Table S4. The information of qPCR primers.
Primer		Sequence (5'→ 3')	Length	Tm	
M. micronuciformi	Mm2F	CTTGTGGTTAATACCCATAAGAA	23	59.3	
	Mega-X	GACTCTGTTTTTGGGGTTT	19	58.9	
A. segnis	A. segnis-F	CGAACGGTAACAGGAGAGAG	20	62.4	
	A. segnis-R	CATGGATGTCAAGAGTAGGT	20	51.7	
S. salivarius	S. salivarius-F	TGAACAAGCRGTWGTCGGTAAC	22	63.7	
	S. salivarius-R	ACTCCGTGTCCAACCAAATC	20	63.3	
NCTC10977_00835
(OCH1)	Forward	ACGCTATATCAAGACGGCGG	20	61.4	
	Reverse	TTACCTTTGCGGCTGGCTAA	20	61.8	
NCTC10977_00376
(RfaF)	Forward	GGCGATTGTTTTGCCGAACT	20	60.0	
	Reverse	AACGCCAATGCCACATAACG	20	59.8	
NCTC10977_00605
(Rfaq_1)	Forward	TTCGCACAATCTTTGGCGTG	20	60.0	
	Reverse	TGGTGTAGCAGAACGACGAC	20	60.0	
NCTC10977_00652
(Rfaq_2)	Forward	AACAGCCTTTCGAGCTTCCA	20	59.9	
	Reverse	CCCGTCTAATACATGCGGCT	20	60.0	
NCTC10977_00728
(Rfaq_3)	Forward	CGACAAGATGGGAAGATTGGC	21	59.3	
	Reverse	TTGCAAGGACTAAGCCGGA	19	58.9	
NCTC10977_00780 
(LpxB)	Forward	CATGGCGTCAGAAACGTGTG	20	60.1	
	Reverse	CACCTCTGAACCACGACTCC	20	60.0	
NCTC10977_00729	Forward	GGGGAAATTGGTTGTGCGAT	20	59.1	
	Reverse	ACTTTATTTAGCGCGGCTGT	20	58.3	
NCTC10977_01271	Forward	CCGCGATTAACACCATGCAG	20	60.0	
	Reverse	CAATACGCTCATCGGCAACG	20	60.0	
 NCTC10977_00649
 (WaaA)	Forward	ACCATCACCACCATGACACC	20	60.0	
	Reverse	ATAAAGCGTGCAACGGCATC	20	59.9	
16S rDNA V3-V4	341-F	CCTAYGGGRBGCASCAG	17	59.3	
	806-R	GGACTACNNGGGTATCTAAT	20	58.9