{"text_format":"3","workbook":"xlsx","n_sheets":6,"sheets":["S5A_12m","S5A_18m","S5B_Speed","S5C_18mRotarod","S5D_RotSum","S5E_18moGrip"],"read":"whole","date_system":"1900"}

{"sheet":"S5A_12m","index":0,"n_rows":73,"n_cols":9,"rows_kept":73}
["Figure S5A Source Data: Statistical analysis results (12mo old distance traveled in an open field)"]
["Table Analyzed","12mo Open Field Distance"]
[]
["Two-way RM ANOVA","Matching: Stacked"]
["    Assume sphericity?","No"]
["    Alpha",0.05]
[]
["Source of Variation","% of total variation","P value","P value summary","Significant?","Geisser-Greenhouse's epsilon"]
["    Time x Genotype",1.521,0.3035,"ns","No"]
["    Time",37.59,"<0.0001","****","Yes",0.4747]
["    Genotype",0.4613,0.5776,"ns","No"]
["    Animal",28.8,"<0.0001","****","Yes"]
[]
["ANOVA table","SS","DF","MS","F (DFn"," DFd)","P value"]
["    Time x Genotype",234607,11,21328,"F (11"," 220) = 1.178","P=0.3035"]
["    Time",5799617,11,527238,"F (5.222"," 104.4) = 29.12","P<0.0001"]
["    Genotype",71175,1,71175,"F (1"," 20) = 0.3204","P=0.5776"]
["    Animal",4442443,20,222122,"F (20"," 220) = 12.27","P<0.0001"]
["    Residual",3982976,220,18104]
[]
["Difference between column means"]
["    Mean of TDP-43wt",459]
["    Mean of TDP-43KQ/KQ",494.3]
["    Difference between means",-35.25]
["    SE of difference",62.28]
["    95% CI of difference","-165.2 to 94.65"]
[]
["Data summary"]
["    Number of columns (Genotype)",2]
["    Number of rows (Time)",12]
["    Number of subjects (Animal)",22]
["    Number of missing values",0]
[]
[]
["Multiple comparisons testing:"]
["Compare each cell mean with the other cell mean in that row"]
[]
["Number of families",1]
["Number of comparisons per family",12]
["Alpha",0.05]
[]
["Holm-Šídák's multiple comparisons test","Mean Diff.","Below threshold?","Summary","Adjusted P Value"]
[]
["  TDP-43wt - TDP-43KQ/KQ"]
[5,-89.81,"No","ns",0.9751]
[10,-18.88,"No","ns",0.998]
[15,-41.88,"No","ns",0.9794]
[20,-7.905,"No","ns",0.998]
[25,43.12,"No","ns",0.9751]
[30,95.41,"No","ns",0.8598]
[35,9.143,"No","ns",0.998]
[40,-17.9,"No","ns",0.998]
[45,-95.58,"No","ns",0.951]
[50,-79.54,"No","ns",0.951]
[55,-148.2,"No","ns",0.3305]
[60,-71.06,"No","ns",0.9706]
[]
[]
["Test details","Mean 1","Mean 2","Mean Diff.","SE of diff.","N1","N2","t","DF"]
[]
["  TDP-43wt - TDP-43KQ/KQ"]
[5,894.3,984.1,-89.81,106.7,15,7,0.8419,19.95]
[10,580.3,599.1,-18.88,79.01,15,7,0.2389,19.63]
[15,511.3,553.1,-41.88,67.11,15,7,0.624,19.24]
[20,509.7,517.6,-7.905,50.37,15,7,0.1569,19.41]
[25,459.3,416.1,43.12,51.27,15,7,0.8411,18.04]
[30,473.3,377.9,95.41,65.68,15,7,1.453,17.95]
[35,449,439.9,9.143,56.67,15,7,0.1613,19.54]
[40,413.5,431.4,-17.9,65.83,15,7,0.2718,16.72]
[45,337.1,432.7,-95.58,80.89,15,7,1.182,11.92]
[50,345.6,425.1,-79.54,70.31,15,7,1.131,16.45]
[55,255.3,403.4,-148.2,64.01,15,7,2.315,17.67]
[60,279.8,350.9,-71.06,75.14,15,7,0.9457,18.42]

{"sheet":"S5A_18m","index":1,"n_rows":73,"n_cols":9,"rows_kept":73}
["Figure S5A Source Data: Statistical analysis results (18mo old distance traveled in an open field)"]
["Table Analyzed","16mo Open Field Distance"]
[]
["Two-way RM ANOVA","Matching: Stacked"]
["    Assume sphericity?","No"]
["    Alpha",0.05]
[]
["Source of Variation","% of total variation","P value","P value summary","Significant?","Geisser-Greenhouse's epsilon"]
["    Time x Genotype",5.987,1E-4,"***","Yes"]
["    Time",37.43,"<0.0001","****","Yes",0.4368]
["    Genotype",0.4014,0.6437,"ns","No"]
["    Animal",28.89,"<0.0001","****","Yes"]
[]
["ANOVA table","SS","DF","MS","F (DFn"," DFd)","P value"]
["    Time x Genotype",804128,11,73103,"F (11"," 176) = 3.595","P=0.0001"]
["    Time",5027772,11,457070,"F (4.804"," 76.87) = 22.47","P<0.0001"]
["    Genotype",53919,1,53919,"F (1"," 16) = 0.2223","P=0.6437"]
["    Animal",3880966,16,242560,"F (16"," 176) = 11.93","P<0.0001"]
["    Residual",3579311,176,20337]
[]
["Difference between column means"]
["    Mean of TDP-43wt",335.8]
["    Mean of TDP-43KQ/KQ",367.6]
["    Difference between means",-31.8]
["    SE of difference",67.44]
["    95% CI of difference","-174.8 to 111.2"]
[]
["Data summary"]
["    Number of columns (Genotype)",2]
["    Number of rows (Time)",12]
["    Number of subjects (Animal)",18]
["    Number of missing values",0]
[]
[]
["Multiple comparisons testing:"]
["Compare each cell mean with the other cell mean in that row"]
[]
["Number of families",1]
["Number of comparisons per family",12]
["Alpha",0.05]
[]
["Holm-Šídák's multiple comparisons test","Mean Diff.","Below threshold?","Summary","Adjusted P Value"]
[]
["  TDP-43wt - TDP-43KQ/KQ"]
[5,-279.7,"No","ns",0.5297]
[10,43.23,"No","ns",0.8616]
[15,94.15,"No","ns",0.8321]
[20,84.5,"No","ns",0.8505]
[25,160.6,"No","ns",0.5297]
[30,-5.6,"No","ns",0.9573]
[35,82.18,"No","ns",0.8505]
[40,-100.3,"No","ns",0.8321]
[45,-89.95,"No","ns",0.8321]
[50,-137.6,"No","ns",0.6106]
[55,-124.3,"No","ns",0.3916]
[60,-108.8,"No","ns",0.587]
[]
[]
["Test details","Mean 1","Mean 2","Mean Diff.","SE of diff.","N1","N2","t","DF"]
[]
["  TDP-43wt - TDP-43KQ/KQ"]
[5,583.4,863.1,-279.7,141.9,10,8,1.971,15.95]
[10,465.1,421.9,43.23,87.02,10,8,0.4967,12.61]
[15,481.4,387.3,94.15,74.6,10,8,1.262,16]
[20,468.5,384,84.5,92.42,10,8,0.9143,12.26]
[25,510.5,349.9,160.6,81.99,10,8,1.959,12.72]
[30,408.9,414.5,-5.6,102.5,10,8,0.05465,12.93]
[35,357.3,275.1,82.18,98.78,10,8,0.8319,11.51]
[40,233.8,334.1,-100.3,82.59,10,8,1.215,14.85]
[45,167.8,257.8,-89.95,76.88,10,8,1.17,15.73]
[50,112.7,250.3,-137.6,80.7,10,8,1.704,13.49]
[55,114.5,238.8,-124.3,54.88,10,8,2.264,13.53]
[60,125.8,234.6,-108.8,60.55,10,8,1.797,14.19]

{"sheet":"S5B_Speed","index":2,"n_rows":51,"n_cols":9,"rows_kept":51}
["Figure S5B Source Data: Statistical analysis results (18mo swim velocity during Morris Water Maze testing)"]
["Table Analyzed","16mo swim speed"]
[]
["Two-way ANOVA","Ordinary"]
["    Alpha",0.05]
[]
["Source of Variation","% of total variation","P value","P value summary","Significant?"]
["    Interaction",10.5,0.058,"ns","No"]
["    Quadrant",6.28,0.1745,"ns","No"]
["    Genotype",0.0111,0.9366,"ns","No"]
[]
["ANOVA table","SS (Type III)","DF","MS","F (DFn"," DFd)","P value"]
["    Interaction",38.2,2,19.1,"F (2"," 48) = 3.02","P=0.0580"]
["    Quadrant",22.9,2,11.4,"F (2"," 48) = 1.81","P=0.1745"]
["    Genotype",0.0403,1,0.0403,"F (1"," 48) = 0.00639","P=0.9366"]
["    Residual",303,48,6.31]
[]
["Difference between column means"]
["    Predicted (LS) mean of TDP-43wt",13.8]
["    Predicted (LS) mean of TDP-43KQ/KQ",13.9]
["    Difference between predicted means",-0.055]
["    SE of difference",0.688]
["    95% CI of difference","-1.44 to 1.33"]
[]
["Data summary"]
["    Number of columns (Genotype)",2]
["    Number of rows (Quadrant)",3]
["    Number of values",54]
[]
[]
["Multiple Comparisons Testing:"]
["Compare each cell mean with the other cell mean in that row"]
[]
["Number of families",1]
["Number of comparisons per family",3]
["Alpha",0.05]
[]
["Holm-Šídák's multiple comparisons test","Predicted (LS) mean diff.","Below threshold?","Summary","Adjusted P Value"]
[]
["  TDP-43wt - TDP-43KQ/KQ"]
["    Visible",-1.98,"No","ns",0.2193]
["    Hidden",-0.32,"No","ns",0.7895]
["    Reversal",2.14,"No","ns",0.2193]
[]
[]
["Test details","Predicted (LS) mean 1","Predicted (LS) mean 2","Predicted (LS) mean diff.","SE of diff.","N1","N2","t","DF"]
[]
["  TDP-43wt - TDP-43KQ/KQ"]
["    Visible",12.4,14.4,-1.98,1.19,10,8,1.66,48]
["    Hidden",14.6,15,-0.32,1.19,10,8,0.268,48]
["    Reversal",14.5,12.3,2.14,1.19,10,8,1.79,48]

{"sheet":"S5C_18mRotarod","index":3,"n_rows":60,"n_cols":9,"rows_kept":60}
["Figure S5C Source Data: Statistical analysis results (18mo Rotarod performance)"]
["Table Analyzed","16mo Rotarod"]
[]
["Two-way RM ANOVA","Matching: Stacked"]
["    Assume sphericity?","No"]
["    Alpha",0.05]
[]
["Source of Variation","% of total variation","P value","P value summary","Significant?","Geisser-Greenhouse's epsilon"]
["    Time x Genotype",1.305,0.6889,"ns","No"]
["    Time",35.48,"<0.0001","****","Yes",0.6184]
["    Genotype",1.423,0.3174,"ns","No"]
["    Animal",22.79,7.6E-3,"**","Yes"]
[]
["ANOVA table","SS","DF","MS","F (DFn"," DFd)","P value"]
["    Time x Genotype",4815,4,1204,"F (4"," 68) = 0.5649","P=0.6889"]
["    Time",130893,4,32723,"F (2.473"," 42.05) = 15.36","P<0.0001"]
["    Genotype",5249,1,5249,"F (1"," 17) = 1.061","P=0.3174"]
["    Animal",84105,17,4947,"F (17"," 68) = 2.322","P=0.0076"]
["    Residual",144896,68,2131]
[]
["Difference between column means"]
["    Mean of TDP-43wt",105.6]
["    Mean of TDP-43KQ/KQ",120.5]
["    Difference between means",-14.89]
["    SE of difference",14.45]
["    95% CI of difference","-45.38 to 15.61"]
[]
["Data summary"]
["    Number of columns (Genotype)",2]
["    Number of rows (Time)",5]
["    Number of subjects (Animal)",19]
["    Number of missing values",0]
[]
[]
["Multiple Comparisons Testing:"]
[]
["Compare each cell mean with the other cell mean in that row"]
[]
["Number of families",1]
["Number of comparisons per family",5]
["Alpha",0.05]
[]
["Šídák's multiple comparisons test","Mean Diff.","95.00% CI of diff.","Below threshold?","Summary","Adjusted P Value"]
[]
["  TDP-43wt - TDP-43KQ/KQ"]
[1,-1.4,"-47.73 to 44.93","No","ns",">0.9999"]
[2,-20.7,"-68.59 to 27.19","No","ns",0.7222]
[3,5.022,"-56.60 to 66.65","No","ns",0.9997]
[4,-32.14,"-104.4 to 40.14","No","ns",0.7023]
[5,-25.21,"-133.2 to 82.78","No","ns",0.969]
[]
[]
["Test details","Mean 1","Mean 2","Mean Diff.","SE of diff.","N1","N2","t","DF"]
[]
["  TDP-43wt - TDP-43KQ/KQ"]
[1,58.6,60,-1.4,15.99,10,9,0.08757,16.56]
[2,79.3,100,-20.7,16.43,10,9,1.26,15.86]
[3,119.8,114.8,5.022,20.37,10,9,0.2466,12.41]
[4,113.3,145.4,-32.14,24.94,10,9,1.289,16.57]
[5,156.9,182.1,-25.21,36.49,10,9,0.691,14.21]

{"sheet":"S5D_RotSum","index":4,"n_rows":52,"n_cols":9,"rows_kept":52}
["Figure S5D Source Data: Statistical analysis results (Mean Rotarod performance across ages)"]
["Table Analyzed","6v9v18mo avg Rotarod"]
[]
["Two-way ANOVA","Ordinary"]
["    Alpha",0.05]
[]
["Source of Variation","% of total variation","P value","P value summary","Significant?"]
["    Interaction",0.2695,0.9246,"ns","No"]
["    Age",2.736,0.4557,"ns","No"]
["    Genotype",2.921,0.1975,"ns","No"]
[]
["ANOVA table","SS (Type III)","DF","MS","F (DFn"," DFd)","P value"]
["    Interaction",569,2,284.5,"F (2"," 55) = 0.07853","P=0.9246"]
["    Age",5777,2,2888,"F (2"," 55) = 0.7973","P=0.4557"]
["    Genotype",6166,1,6166,"F (1"," 55) = 1.702","P=0.1975"]
["    Residual",199249,55,3623]
[]
["Difference between column means"]
["    Predicted (LS) mean of TDP-43wt",152.7]
["    Predicted (LS) mean of TDP-43KQ/KQ",173.2]
["    Difference between predicted means",-20.53]
["    SE of difference",15.73]
["    95% CI of difference","-52.06 to 11.00"]
[]
["Data summary"]
["    Number of columns (Genotype)",2]
["    Number of rows (Age)",3]
["    Number of values",61]
[]
[]
["Multiple Comparisons Testing:"]
[]
["Compare each cell mean with the other cell mean in that row"]
[]
["Number of families",1]
["Number of comparisons per family",3]
["Alpha",0.05]
[]
["Holm-Šídák's multiple comparisons test","Predicted (LS) mean diff.","Below threshold?","Summary","Adjusted P Value"]
[]
["  TDP-43wt - TDP-43KQ/KQ"]
[6,-13.44,"No","ns",0.7242]
[9,-19.46,"No","ns",0.7242]
[18,-28.68,"No","ns",0.6633]
[]
[]
["Test details","Predicted (LS) mean 1","Predicted (LS) mean 2","Predicted (LS) mean diff.","SE of diff.","N1","N2","t","DF"]
[]
["  TDP-43wt - TDP-43KQ/KQ"]
[6,159.5,172.9,-13.44,27.05,13,8,0.4968,55]
[9,163.5,183,-19.46,27.05,13,8,0.7196,55]
[18,135.1,163.8,-28.68,27.65,10,9,1.037,55]

{"sheet":"S5E_18moGrip","index":5,"n_rows":69,"n_cols":9,"rows_kept":69}
["Figure S5E Source Data: Statistical analysis results (Mean grip strength by forcemeter at 18mo)"]
["Table Analyzed","16mo Avg GripStrengthMeter"]
[]
["Two-way RM ANOVA","Matching: Stacked"]
["    Assume sphericity?","Yes"]
["    Alpha",0.05]
[]
["Source of Variation","% of total variation","P value","P value summary","Significant?"]
["    Time x Genotype",0.4415,0.2313,"ns","No"]
["    Time",87.62,"<0.0001","****","Yes"]
["    Genotype",0.537,0.2201,"ns","No"]
["    Animal",5.632,0.3837,"ns","No"]
[]
["ANOVA table","SS","DF","MS","F (DFn"," DFd)","P value"]
["    Time x Genotype",0.05408,1,0.05408,"F (1"," 17) = 1.541","P=0.2313"]
["    Time",10.73,1,10.73,"F (1"," 17) = 305.9","P<0.0001"]
["    Genotype",0.06579,1,0.06579,"F (1"," 17) = 1.621","P=0.2201"]
["    Animal",0.69,17,0.04059,"F (17"," 17) = 1.157","P=0.3837"]
["    Residual",0.5964,17,0.03508]
[]
["Difference between row means"]
["    Mean of Forepaws",1.126]
["    Mean of All paws",2.191]
["    Difference between means",-1.064]
["    SE of difference",0.06086]
["    95% CI of difference","-1.193 to -0.9361"]
[]
["Difference between column means"]
["    Mean of TDP-43wt",1.7]
["    Mean of TDP-43KQ/KQ",1.617]
["    Difference between means",0.08333]
["    SE of difference",0.06545]
["    95% CI of difference","-0.05476 to 0.2214"]
[]
["Interaction CI"]
["    Mean diff"," A1 - B1",0.007778]
["    Mean diff"," A2 - B2",0.1589]
["    (A1 -B1) - (A2 - B2)",-0.1511]
["    95% CI of difference","-0.4079 to 0.1057"]
["    (B1 - A1) - (B2 - A2)",0.1511]
["    95% CI of difference","-0.1057 to 0.4079"]
[]
["Data summary"]
["    Number of columns (Genotype)",2]
["    Number of rows (Time)",2]
["    Number of subjects (Animal)",19]
["    Number of missing values",0]
[]
[]
["Multiple Comparisons Testing:"]
[]
["Compare each cell mean with the other cell mean in that row"]
[]
["Number of families",1]
["Number of comparisons per family",2]
["Alpha",0.05]
[]
["Šídák's multiple comparisons test","Predicted (LS) mean diff.","95.00% CI of diff.","Below threshold?","Summary","Adjusted P Value"]
[]
["  TDP-43wt - TDP-43KQ/KQ"]
["    Forepaws",0.007778,"-0.2013 to 0.2169","No","ns",0.9953]
["    All paws",0.1589,"-0.05020 to 0.3680","No","ns",0.1616]
[]
[]
["Test details","Predicted (LS) mean 1","Predicted (LS) mean 2","Predicted (LS) mean diff.","SE of diff.","N1","N2","t","DF"]
[]
["  TDP-43wt - TDP-43KQ/KQ"]
["    Forepaws",1.13,1.122,0.007778,0.08937,10,9,0.08702,34]
["    All paws",2.27,2.111,0.1589,0.08937,10,9,1.778,34]