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["Supplementary Table S2. List of primer sets used in PCR"]
[]
["Gene name",null,"Sequence"]
["Genomic PCR & sequencing"]
[]
["hADAM17-ex14","Forward","5´-ATGAGCACACACACAAACGC-3´"]
[null,"Reverse","5´-CCTGTGGCCTGTGAAGATGT-3´"]
["hADAM17-ex15","Forward","5´-CCCTGAGGGGCACACTTTAG-3´"]
[null,"Reverse","5´-CAAAGCCTCCCACCACAGAA-3´"]
[]
["RT-PCR & sequencing"]
[null,"Forward","5´-GTGATCCTGGCATCATGTATCTG-3´"]
["hADAM17","Reverse","5´-CCATGCTGCTCAGCATTTCGACG-3´"]
[]
["qRT-PCR"]
["syn-hADAM17","Forward","5´-GATCTGACCGATCATCCTGTG-3´"]
[null,"Reverse a","5´-GTAATCCTCGAGGCATTCGG-3´ "," "]
[null,"Probe","5´-AGAACCGCGTGGACAGCAAAGAAA-3´"]
[]
["Actb (b-actin)","Forward","5´-GGCTGTATTCCCCTCCATCG-3´"]
[null,"Reverse","5´-CCAGTTGGTAACAATGCCATGT-3´"]
[]
["Mutagenesis for syn-hADAM17"]
["syn-hADAM17-C567R","Forwardb","5´-CGCCTGGATCTGGGCAAATGCAAA-3´ "]
[null,"Reverse","5´-CACGGTATCATCTTCGGCATTGCCA-3´ "]
["syn-hADAM17-C600Y","Forwardc","5´-ATAAAGTTTGCTGCCGCGATCTGTC-3´ "]
[null,"Reverse","5´-AGCTATTATCGGTTTCGTTGCAGGCG-3´ "]
[]
["Construction of expression plasmid for HiBiT-tagged syn-hADAM17"]
[null,"Forwardd","5´-GTGAGCGGCTGGCGGCTGTTCAAGAAGATTAGC\n     GATGAAGGCGAAGAATGCGATCCTGGCATCATGTA-3´ "]
[null,"Reverse","5´-CACACGGCTATTGCCGCAAACTTTGTTGCTGCGC\n     TCTTGAAAGCACTC-3´"]
["aSequence of FLAG-tag"]
["bMutated nucleotide sequence for syn-hADAM17-C567R was underlined."]
["cMutated nucleotide sequence for syn-hADAM17-C600Y  was underlined."]
["cInserted  nucleotide sequence for HiBit-tag was underlined."]