LOCUS       Exported               16797 bp DNA     circular SYN 05-MAY-2022
DEFINITION  Plasmid containing Cas9 and three sgRNA constructs targeting lemon 
            white1 gene in Miscanthus. This plasmid is derived from pPTN1399 
            which was obtained from Dr. Tom Clemente at University of Nebraska-
            Lincoln.
ACCESSION   <unknown
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 5, 2022 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16797
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    complement(join(1..136,16775..16797))
                     /label=CaMV poly(A) signal
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=98.137"
                     /note="/match_length=91.47727272727273"
                     /note="/other=polyA signal"
     misc_feature    complement(138..157)
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.84210526315789"
                     /note="/other=misc feature"
     CDS             161..247
                     /codon_start=1
                     /label=lacZ
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=2.861788617886179"
                     /note="/other=CDS"
                     /translation="HWPSFYNVVTGKTLALPNLIALQHIPLSP"
     promoter        267..712
                     /label=OsU6a promoter
                     /note="/created_by=mbelaffif"
     misc_feature    713..732
                     /label=lw1G1
                     /note="/created_by=mbelaffif"
                     /note="/modified_by=mbelaffif"
     misc_RNA        733..807
                     /label=gRNA scaffold
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc RNA"
     protein_bind    complement(820..849)
                     /label=attL2
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=31.0"
                     /note="/other=protein bind"
     promoter        850..1181
                     /label=OsU6b promoter
                     /note="/created_by=mbelaffif"
                     /note="/modified_by=mbelaffif"
     misc_feature    1183..1202
                     /label=lw1G2
                     /note="/created_by=mbelaffif"
                     /note="/modified_by=mbelaffif"
     misc_RNA        1203..1277
                     /label=gRNA scaffold
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc RNA"
     protein_bind    complement(1290..1318)
                     /label=attL2
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=30.0"
                     /note="/other=protein bind"
     promoter        1320..1681
                     /label=TaU6
                     /note="/created_by=mbelaffif"
     misc_feature    1682..1701
                     /label=lw1G3
                     /note="/created_by=mbelaffif"
                     /note="/modified_by=mbelaffif"
     misc_RNA        1702..1776
                     /label=gRNA scaffold
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc RNA"
     protein_bind    complement(1789..1817)
                     /label=attL2
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=30.0"
                     /note="/other=protein bind"
     misc_feature    1827..1844
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=33.33333333333333"
                     /note="/other=misc feature"
     protein_bind    1876..1897
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=95.83333333333334"
                     /note="/other=protein bind"
     promoter        complement(1902..1931)
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=96.875"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1947..1967
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein bind"
     CDS             complement(1981..2068)
                     /codon_start=1
                     /label=lacI
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.21791320406279"
                     /note="/other=CDS"
                     /translation="QYANRLSPRVGRFINAAGTTGFPTGKRAV"
     promoter        2159..2834
                     /label=CaMV 35S promoter (enhanced)
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             2898..3694
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    complement(3752..3925)
                     /label=CaMV poly(A) signal
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.432"
                     /note="/match_length=100.0"
                     /note="/other=polyA signal"
     misc_feature    complement(4004..4027)
                     /label=LB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc feature"
     promoter        4285..4309
                     /label=Pc promoter
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.65517241379311"
                     /note="/other=promoter"
     CDS             4486..4541
                     /codon_start=1
                     /label=GmR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=96.491"
                     /note="/match_length=10.674157303370785"
                     /note="/other=CDS"
                     /translation="MLWSSNDVTQQGSRPKTK"
     CDS             4549..5339
                     /codon_start=1
                     /label=SmR
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5586..6173
                     /label=ori
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    6360..6499
                     /label=bom
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.296"
                     /note="/match_length=100.0"
                     /note="/other=misc feature"
     CDS             complement(6602..6640)
                     /codon_start=1
                     /label=rop
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=21.164021164021165"
                     /note="/other=CDS"
                     /translation="CLARFGDDGENL"
     rep_origin      complement(6844..7037)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=99.49"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     regulatory      complement(7104..8176)
                     /label=pVS1 RepA
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/modified_by=mbelaffif"
                     /note="/other=CDS"
     regulatory      complement(8606..9234)
                     /label=pVS1 StaA
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/modified_by=mbelaffif"
                     /note="/other=CDS"
     misc_feature    complement(10546..10569)
                     /label=RB T-DNA repeat
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc feature"
     CDS             complement(10634..10789)
                     /codon_start=1
                     /label=lacZ
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.105691056910569"
                     /note="/other=CDS"
                     /translation="TGRRFTTS*LGKPWRYPT*SPCSTSPFRQLA**RRGPHRSPFPTV
                     AQPEWRM"
     promoter        10799..12538
                     /label=SobicUBI4
                     /note="/created_by=mbelaffif"
     misc_feature    12569..16671
                     /label=Cas9
                     /label=nonstandard type: swissprot
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/modified_by=mbelaffif"
                     /note="/other=swissprot"
     CDS             16679..16698
                     /codon_start=1
                     /label=SV40 NLS
                     /note="pLannotate"
                     /note="/database=addgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="PKKKRK"
ORIGIN
        1 agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa
       61 gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc
      121 taaaaccaaa atccagtgac ctgcaggcat gcaagcttgg cactggccgt cgttttacaa
      181 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      241 ttcgccaggg ggatccccat ttaaattttt ttcctgtagt tttcccacaa ccatttttta
      301 ccatccgaat gataggatag gaaaaatatc caagtgaaca gtattcctat aaaattcccg
      361 taaaaagcct gcaatccgaa tgagccctga agtctgaact agccggtcac ctgtacaggc
      421 tatcgagatg ccatacaaga gacggtagta ggaactagga agacgatggt tgattcgtca
      481 ggcgaaatcg tcgtcctgca gtcgcatcta tgggcctgga cggaataggg gaaaaagttg
      541 gccggatagg agggaaaggc ccaggtgctt acgtgcgagg taggcctggg ctctcagcac
      601 ttcgattcgt tggcaccggg gtaggatgca atagagagca acgtttagta ccacctcgct
      661 tagctagagc aaactggact gccttatatg cgcgggtgct ggcttggctg ccggaggcct
      721 acggattctg ctgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
      781 acttgaaaaa gtggcaccga gtcggtgctt tttttctaga cccagctttc ttgtacaaag
      841 ttggcattat gcaagaacga actaagccgg acaaaaaaaa aaggagcaca tatacaaacc
      901 ggttttattc atgaatggtc acgatggatg atggggctca gacttgagct acgaggccgc
      961 aggcgagaga agcctagtgt gctctctgct tgtttgggcc gtaacggagg atacggccca
     1021 cgagcgtgta ctaccgcgcg ggatgccgct gggcgctgcg ggggccgttg gatggggatc
     1081 ggtgggtcgc gggagcgttg aggggagaca ggtttagtac cacctcgcct accgaacaat
     1141 gaagaaccca ccttataacc ccgcgcgctg ccgcttgtgt tgtgttgata cgacctgccc
     1201 ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     1261 gtggcaccga gtcggtgctt tttttctaga cccagctttc ttgtacaaag ttggcattag
     1321 accaagcccg ttattctgac agttctggtg ctcaacacat ttatatttat caaggagcac
     1381 attgttactc actgctagga gggaatcgaa ctaggaatat tgatcagagg aactacgaga
     1441 gagctgaaga taactgccct ctagctctca ctgatctggg tcgcatagtg agatgcagcc
     1501 cacgtgagtt cagcaacggt ctagcgctgg gcttttaggc ccgcatgatc gggcttttgt
     1561 cgggtggtcg acgtgttcac gattggggag agcaacgcag cagttcctct tagtttagtc
     1621 ccacctcgcc tgtccagcag agttctgacc ggtttataaa ctcgcttgct gcatcagact
     1681 tgaaatcatt cctgttgatg cgttttagag ctagaaatag caagttaaaa taaggctagt
     1741 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttctagac ccagctttct
     1801 tgtacaaagt tggcattaat ttaaatgggt accgagctcg aattcgtaat catgtcatag
     1861 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     1921 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1981 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2041 cgcgcgggga gaggcggttt gcgtattggc tagagcagct tgccaacatg gtggagcacg
     2101 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg
     2161 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
     2221 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
     2281 ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc
     2341 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     2401 attgatgtga acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
     2461 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
     2521 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
     2581 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
     2641 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
     2701 ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac
     2761 gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg
     2821 gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc gagctttcgc
     2881 agatctgtcg atcgaccatg gggattgaac aagatggatt gcacgcaggt tctccggccg
     2941 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     3001 ccgccgtgtt tcggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3061 ccggtgccct gaatgaactc caggacgagg cagcgcggct atcgtggctg gccacgacgg
     3121 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3181 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     3241 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     3301 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     3361 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     3421 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc
     3481 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     3541 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     3601 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     3661 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcggatcgat
     3721 cctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc
     3781 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
     3841 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     3901 caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtacatta
     3961 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
     4021 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
     4081 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
     4141 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
     4201 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
     4261 tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     4321 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     4381 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     4441 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     4501 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg
     4561 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     4621 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     4681 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     4741 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     4801 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     4861 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     4921 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     4981 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     5041 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     5101 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     5161 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     5221 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     5281 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     5341 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
     5401 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
     5461 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa
     5521 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5581 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5641 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5701 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     5761 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5821 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5881 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5941 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6001 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6061 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6121 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6181 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6241 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6301 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6361 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     6421 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     6481 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6541 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6601 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc
     6661 cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg
     6721 ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg
     6781 gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac
     6841 agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg
     6901 gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg
     6961 tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca
     7021 atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt
     7081 tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg
     7141 cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc
     7201 ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg
     7261 gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg
     7321 gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg
     7381 gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca
     7441 gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg
     7501 ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta
     7561 cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc
     7621 tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac
     7681 acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg
     7741 gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg
     7801 gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca
     7861 cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg
     7921 tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc
     7981 gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct
     8041 tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg
     8101 gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat
     8161 ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc
     8221 ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt
     8281 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa
     8341 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat
     8401 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc
     8461 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac
     8521 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg
     8581 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg
     8641 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg
     8701 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt
     8761 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca
     8821 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca
     8881 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt
     8941 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta
     9001 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga
     9061 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat
     9121 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg
     9181 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt
     9241 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa
     9301 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc
     9361 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag
     9421 ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc
     9481 ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct
     9541 tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc
     9601 tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt
     9661 gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg
     9721 ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg
     9781 gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg
     9841 attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc
     9901 ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg
     9961 atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc
    10021 actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg
    10081 cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg
    10141 gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca
    10201 gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt
    10261 atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc
    10321 gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag
    10381 ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttaaagtac tttgatcccg
    10441 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
    10501 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
    10561 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gatccaagct
    10621 caagctgctc tagcattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    10681 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    10741 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttgc
    10801 atgcctgcat gattgaagca aacggtatag caacggtgtt aacctgatct agtgatctct
    10861 tgcaatcctt aacggccacc taccgcaggt agcaaacggc gtccccctcc tcgatatctc
    10921 cgcggcgacc tctggctttt tccgcggaat tgcgcggtgg ggacggattc cacgagaccg
    10981 cgacgcaacc gcctctcgcc gctgggcccc acaccgctcg gtgccgtagc ctcacgggac
    11041 tctttctccc tcctcccccg ttataaattg gcttcatccc ctccttgcct catccatcca
    11101 aatcccagtc cccaatccca tcccttcgtc ggagaaattc atcgaagcga agcgaatcct
    11161 cgcgatcctc tcaaggtact gcgagttttc gatccccctc tcgacccctc gtatgtttgt
    11221 gtttgtcgta gcgtttgatt aggtatgctt tccctgtttg tgttcgtcgt agcgtttgat
    11281 taggtatgct ttccctgttc gtgttcatcg tagtgtttga ttaggtcgtg tgaggcgatg
    11341 gcctgctcgc gtccttcgat ctgtagtcga tttgcgggtc gtggtgtaga tctgcgggct
    11401 gtgatgaagt tatttggtgt gatctgctcg cctgattctg cgggttggct cgagtagata
    11461 tgatggttgg accggttggt tcgtttaccg cgctagggtt gggctgggat gatgttgcat
    11521 gcgccgttgc gcgtgatccc gcagcaggac ttgcgtttga ttgccagatc tcgttacgat
    11581 tatgtgattt ggtttggact ttttagatct gtagcttctg cttatgtgcc agatgcgcct
    11641 actgctcata tgcctgatga taatcataaa tggctgtgga actaactagt tgattgcgga
    11701 gtcatgtatc agctacaggt gtagggacta gctacaggtg tagggacttg cgtctaattg
    11761 tttggtcctt tactcatgtt gcaattatgc aatttagttt agattgtttg ttccactcat
    11821 ctaggctgta aaagggacac tgcttagatt gctgtttaat ctttttagta gattatatta
    11881 tattggtaac ttattacccc tattacatgc catacgtgac ttctgctcat gcctgatgat
    11941 aatcatagat cactgtggaa ttaattagtt gattgttgaa tcatgtttca tgtacatacc
    12001 acggcacaat tgcttagttc cttaacaaat gcaaatttta ctgatccatg tatgatttgc
    12061 gtggttctct aatgtgaaat actatagcta cttgttagta agaatcaggt tcgtatgctt
    12121 aatgctgtat gtgccttctg ctcatgcctg atgataatca tatatcactg gaattaatta
    12181 gttgatcgtt taatcatata tcaagtacat accatggcac aatttttagt cacttaaccc
    12241 atgcagattg aactggtccc tgcatgtttt gctaaattgt tctattctga ttagaccata
    12301 tatcatgtat ttttttttgg taatggttct cttattttaa atgctatata gttctggtac
    12361 ttgttagaaa gatctgcttc atagtttagt tgcctatccc tcgaattagg atgctgagca
    12421 gccgatccta tagctttgtt tcatgtatca attcttttgt gttcaacagt cagtttttgt
    12481 tagattcatt gtaacttatg gtcgcttact cttctggtcc tcaatgcttg caggaattcg
    12541 agctcggtac ctcgcgaatg catctagaat ggacaagaag tactccatcg gcctcgatat
    12601 cggcacaaac agcgtgggct gggccgtcat caccgacgag tacaaggtgc cttccaagaa
    12661 gttcaaggtc ctgggcaaca ccgatcgcca cagcatcaag aagaatctca ttggagctct
    12721 cctgttcgac tccggagaga cagccgaggc tacaaggctc aagcgcaccg ctaggcgcag
    12781 gtacacacgc aggaagaata ggatctgcta cctgcaggag atcttctcca acgagatggc
    12841 caaggtggac gattccttct tccaccgcct ggaggagagc ttcctcgtcg aggaagacaa
    12901 gaagcacgag aggcacccta tcttcggcaa catcgtggac gaggtcgcct accacgagaa
    12961 gtaccccaca atctaccacc tgaggaagaa gctcgtggac tccaccgata aggctgacct
    13021 ccgcctgatc tacctcgccc tggctcacat gatcaagttc aggggccact tcctgatcga
    13081 gggcgatctc aacccagaca atagcgatgt ggacaagctg ttcatccagc tcgtccagac
    13141 atacaatcag ctcttcgagg agaacccgat caatgcctcc ggcgtggacg ccaaggctat
    13201 cctgagcgct aggctctcca agagccgcag gctcgagaac ctgatcgccc agctgccagg
    13261 cgagaagaag aacggcctgt tcggcaatct catcgctctc tccctgggcc tcacaccgaa
    13321 cttcaagagc aatttcgatc tcgccgagga cgctaagctg cagctctcca aggacaccta
    13381 cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgctg acctgttcct
    13441 cgccgctaag aatctgtccg acgccatcct cctgagcgat atcctccgcg tgaacaccga
    13501 gatcacaaag gccccactct ccgctagcat gatcaagcgc tacgacgagc accaccagga
    13561 tctgaccctc ctgaaggctc tggtcaggca gcagctcccg gagaagtaca aggagatctt
    13621 cttcgatcag agcaagaacg gctacgctgg atacattgat ggcggcgctt cccaagagga
    13681 gttctacaag ttcatcaagc cgatcctgga gaagatggac ggcacagagg agctcctggt
    13741 gaagctcaat agggaggacc tcctgaggaa gcagcgcacc ttcgataacg gcagcatccc
    13801 tcaccagatt cacctcggag agctgcatgc tatcctgcgc aggcaagagg acttctaccc
    13861 cttcctcaag gataacaggg agaagattga gaagatcctg acattccgca ttccttacta
    13921 cgtgggacct ctggctaggg gaaattcccg cttcgcttgg atgacccgca agagcgagga
    13981 gacaatcaca ccttggaact tcgaggaagt ggtcgataag ggcgcctccg ctcagagctt
    14041 catcgagagg atgacaaatt tcgacaagaa cctgcctaat gagaaggtgc tccccaagca
    14101 ctccctcctg tacgagtact tcaccgtcta caacgagctc acaaaggtga agtatgtgac
    14161 cgagggaatg aggaagcctg ccttcctgag cggagagcag aagaaggcta tcgtggacct
    14221 cctgttcaag accaatagga aggtgacagt caagcagctc aaggaagact acttcaagaa
    14281 gatcgagtgc ttcgattccg tggagatcag cggcgtcgag gacaggttca acgcctccct
    14341 gggcacctac cacgatctcc tgaagatcat caaggataag gacttcctgg acaacgagga
    14401 gaatgaggat atcctcgagg acatcgtgct gaccctcaca ctgttcgagg atagggagat
    14461 gatcgaggag cgcctgaaga catacgccca cctcttcgat gacaaggtca tgaagcagct
    14521 gaagcgcagg cgctacacag gatggggcag gctgagccgc aagctcatca atggcatccg
    14581 cgacaagcag tccggcaaga caatcctcga cttcctgaag agcgatggct tcgctaacag
    14641 gaatttcatg cagctgatcc acgatgactc cctcaccttc aaggaagaca ttcagaaggc
    14701 tcaggtgtcc ggacagggcg acagcctgca cgagcacatc gccaacctcg ctggcagccc
    14761 tgctatcaag aagggcatcc tgcagaccgt gaaggtggtg gacgagctcg tgaaggtcat
    14821 gggcaggcac aagcccgaga acatcgtcat cgagatggcc cgcgagaatc agaccacaca
    14881 gaagggccag aagaactcca gggagcgcat gaagcgcatc gaggaaggca tcaaggagct
    14941 gggcagccag atcctcaagg agcacccagt ggagaacaca cagctgcaga atgagaagct
    15001 ctacctgtac tacctccaga atggcaggga tatgtatgtg gaccaggagc tggatatcaa
    15061 ccgcctctcc gattacgacg tggatcacat cgtccctcag tccttcctga aggatgacag
    15121 catcgacaat aaggtgctca ccaggagcga caagaaccgc ggcaagtccg ataatgtccc
    15181 cagcgaggaa gtggtcaaga agatgaagaa ctactggagg cagctcctga atgccaagct
    15241 gatcacacag aggaagttcg ataacctcac aaaggctgag cgcggcggcc tctccgagct
    15301 ggacaaggct ggcttcatca agaggcagct ggtggagaca cgccagatca caaagcacgt
    15361 cgctcagatc ctcgacagcc gcatgaacac aaagtacgat gagaatgaca agctgatcag
    15421 ggaggtgaag gtcatcaccc tgaagtccaa gctcgtgagc gacttcagga aggatttcca
    15481 gttctacaag gtccgcgaga tcaacaatta ccaccacgcc cacgacgctt acctcaacgc
    15541 cgtggtcggc acagctctga tcaagaagta cccaaagctc gagtccgagt tcgtgtacgg
    15601 cgactacaag gtgtacgatg tcaggaagat gatcgccaag tccgagcagg agatcggcaa
    15661 ggccaccgct aagtacttct tctacagcaa catcatgaat ttcttcaaga ccgagatcac
    15721 actggccaat ggcgagatca ggaagcgccc gctcatcgag acaaacggcg agacaggcga
    15781 gatcgtgtgg gacaagggca gggatttcgc taccgtgcgc aaggtcctct ccatgcctca
    15841 agtgaatatc gtcaagaaga ccgaagtcca gacaggcggc ttctccaagg agagcatcct
    15901 gcccaagagg aacagcgata agctcatcgc ccgcaagaag gactgggatc caaagaagta
    15961 cggtggcttc gactccccga cagtggccta cagcgtcctg gtggtcgcta aggtggagaa
    16021 gggcaagtcc aagaagctca agagcgtcaa ggagctcctg ggcatcacca tcatggagcg
    16081 ctccagcttc gagaagaatc caatcgattt cctggaggcc aagggctaca aggaagtgaa
    16141 gaaggacctg atcatcaagc tcccgaagta cagcctcttc gagctggaga acggcaggaa
    16201 gcgcatgctc gcttccgctg gagagctcca gaagggcaat gagctcgccc tgccatccaa
    16261 gtatgtgaac ttcctctacc tggcttccca ctacgagaag ctcaagggca gcccggagga
    16321 taacgagcag aagcagctgt tcgtcgagca gcacaagcac tacctcgacg agatcatcga
    16381 gcagatctcc gagttctcca agcgcgtgat cctggccgac gctaatctgg ataaggtcct
    16441 ctccgcctac aacaagcaca gggataagcc tatccgcgag caggctgaga atatcatcca
    16501 cctcttcacc ctgacaaacc tcggagctcc tgctgctttc aagtacttcg acaccacaat
    16561 cgataggaag cgctacacca gcacaaagga agtgctcgac gctaccctca tccaccagtc
    16621 catcaccggc ctctacgaga caaggattga cctgagccag ctcggcggcg atctgcagcc
    16681 aaagaagaag aggaaggtcg gcggctgagt cgagcatgca tctagagtcc gcaaaaatca
    16741 ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagt
//
