Project info 1 Experiment Date2021-03-24 Experiment ID Notebook ID Project Experimenter Protocol Figure1D 10 30 50 70 90 110 130 150 170 190 <Subcolumn> <d>4.24</d> <d>9.33</d> <d>15.56</d> <d>18.03</d> <d>20.13</d> <d>21.33</d> <d>16.4</d> <d>12.13</d> <d>8.54</d> <d>3.22</d> </Subcolumn> </YColumn> </Table> <Table ID="Table1" XFormat="none" YFormat="SDN" TableType="TwoWay" EVFormat="AsteriskAfterNumber"> <Title>Figure1E Hsp70 CD63 LAMP2 calnexin Cell 0.213 0.345 0.173 0.845 0.025 0.036 0.023 0.086 3 3 3 3 EVs 0.832 1.213 0.764 0.243 0.085 0.117 0.079 0.026 3 3 3 3
Figure1E-1 Hsp70 Cell 0.186 0.218 0.235 EVs 0.93 0.775 0.792
Figure1E-2 CD63 Cell 0.324 0.324 0.387 EVs 1.244 1.311 1.084
Figure1E-3 LAMP2 Cell 0.191 0.147 0.181 EVs 0.755 0.69 0.847
Figure1E-4 calnexin Cell 0.934 0.839 0.762 EVs 0.271 0.238 0.22
Figure1G-1 0 24 48 72 96 Control 0 0.265 0.503 0.765 0.895 0 0.027 0.048 0.074 0.088 3 3 3 3 3 M2-CM 0 0.532 0.924 1.364 1.532 0 0.056 0.089 0.141 0.146 3 3 3 3 3 M2-EVs 0 0.603 1.043 1.488 1.703 0 0.061 0.102 0.145 0.171 3 3 3 3 3
Figure1G-2 0 24 48 72 96 Control 0 0.201 0.384 0.589 0.706 0 0.022 0.036 0.061 0.073 3 3 3 3 3 M2-CM 0 0.583 1.094 1.543 1.674 0 0.061 0.105 0.155 0.171 3 3 3 3 3 M2-EVs 0 0.657 1.148 1.654 1.803 0 0.067 0.117 0.166 0.178 3 3 3 3 3
Figure1H ACHN 769-P Control 64.33 86.67 6.35 8.96 3 3 M2-CM 134.33 157.33 13.58 15.01 3 3 M2-EVs 146.33 171 14.98 16.82 3 3
Figure1H-1 ACHN Control 57 68 68 M2-CM 147 136 120 M2-EVs 142 163 134
Figure1H-2 769-P Control 81 97 82 M2-CM 172 142 158 M2-EVs 190 158 165
Figure1I ACHN 769-P Control 43 32 4.36 3.61 3 3 M2-CM 78.33 67.67 8.14 6.81 3 3 M2-EVs 85.67 70 8.5 7 3 3
Figure1I-1 ACHN Control 48 41 40 M2-CM 82 69 84 M2-EVs 89 76 92
Figure1I-2 769-P Control 33 35 28 M2-CM 60 70 73 M2-EVs 67 78 65
Figure1J-1 E-cadherin Vimentin Control 0.874 0.674 0.085 0.068 3 3 M2-CM 0.324 1.232 0.034 0.117 3 3 M2-EVs 0.256 1.343 0.027 0.132 3 3
Figure1J-1-1 E-cadherin Control 0.825 0.825 0.972 M2-CM 0.289 0.357 0.326 M2-EVs 0.256 0.283 0.229
Figure1J-1-2 Vimentin Control 0.742 0.606 0.674 M2-CM 1.172 1.157 1.367 M2-EVs 1.481 1.329 1.218
Figure1J-2 E-cadherin Vimentin Control 1.435 0.218 0.136 0.023 3 3 M2-CM 0.644 0.801 0.062 0.079 3 3 M2-EVs 0.432 0.835 0.041 0.085 3 3
Figure1J-2-1 E-cadherin Control 1.279 1.531 1.494 M2-CM 0.588 0.711 0.634 M2-EVs 0.476 0.426 0.394
Figure1J-2-2 Vimentin Control 0.192 0.233 0.229 M2-CM 0.76 0.751 0.892 M2-EVs 0.826 0.755 0.924
Figure2E HUVECs-EVs 1 0.893 1.114 Cy3-EVs 1.09 1.326 1.195 THP-1-EVs 1.498 1.65 1.81 M2-EVs 1.806 1.667 2.029
Figure2G-1 Control 1 0.916 1.101 M2-CM 1.847 1.579 1.926 M2-EVs 2.336 1.976 2.36
Figure2G-2 Control 1 1.086 0.884 M2-CM 1.669 1.369 1.6 M2-EVs 1.6 1.866 1.556
Figure3A anti NC 1 1.137 0.907 anti miR-342-3p 0.217 0.271 0.242
Figure3B-1 Control 1 0.902 1.051 M2-EVs-antiNC 1.578 1.843 1.931 M2-EVs-anti miR-342-3p 1.055 1.17 1.297
Figure3B-2 Control 1 0.933 1.107 M2-EVs-antiNC 1.488 1.803 1.731 M2-EVs-anti miR-342-3p 0.782 0.932 0.947
Figure3C-1 0 24 48 72 96 Control 0 0.203 0.446 0.711 0.806 0 0.018 0.046 0.068 0.078 3 3 3 3 3 M2-EVs-antiNC 0 0.557 1.034 1.434 1.646 0 0.057 0.101 0.138 0.166 3 3 3 3 3 M2-EVs-anti miR-342-3p 0 0.245 0.511 0.785 0.932 0 0.025 0.048 0.081 0.092 3 3 3 3 3
Figure3C-2 0 24 48 72 96 Control 0 0.234 0.534 0.704 0.823 0 0.024 0.052 0.069 0.083 3 3 3 3 3 M2-EVs-antiNC 0 0.665 1.204 1.687 1.803 0 0.067 0.117 0.171 0.181 3 3 3 3 3 M2-EVs-anti miR-342-3p 0 0.305 0.611 0.834 0.954 0 0.028 0.058 0.084 0.096 3 3 3 3 3
Figure3D ACHN 769-P Control 72.33 63 6.81 6.08 3 3 M2-EVs-antiNC 174 154.33 16.82 14.64 3 3 M2-EVs-anti miR-342-3p 90 72 9.17 7 3 3
Figure3D-1 ACHN Control 67 70 80 M2-EVs-antiNC 180 155 187 M2-EVs-anti miR-342-3p 92 80 98
Figure3D-2 769-P Control 67 56 66 M2-EVs-antiNC 170 152 141 M2-EVs-anti miR-342-3p 79 72 65
Figure3E ACHN 769-P Control 26.67 41 2.89 4.36 3 3 M2-EVs-antiNC 78.33 98.33 8.08 10.26 3 3 M2-EVs-anti miR-342-3p 35.33 52 3.21 5 3 3
Figure3E-1 ACHN Control 29 23 27 M2-EVs-antiNC 70 87 78 M2-EVs-anti miR-342-3p 32 35 39
Figure3E-2 769-P Control 40 38 46 M2-EVs-antiNC 87 107 101 M2-EVs-anti miR-342-3p 55 46 54
Figure3F-1 E-cadherin Vimentin Control 1.342 0.533 0.127 0.054 3 3 M2-EVs-antiNC 0.335 1.435 0.035 0.136 3 3 M2-EVs-anti miR-342-3p 1.243 0.613 0.128 0.062 3 3
Figure3F-1-1 E-cadherin Control 1.278 1.26 1.488 M2-EVs-antiNC 0.374 0.324 0.307 M2-EVs-anti miR-342-3p 1.387 1.141 1.202
Figure3F-1-2 Vimentin Control 0.474 0.546 0.579 M2-EVs-antiNC 1.459 1.289 1.558 M2-EVs-anti miR-342-3p 0.545 0.628 0.666
Figure3F-2 E-cadherin Vimentin Control 0.843 0.752 0.087 0.072 3 3 M2-EVs-antiNC 0.264 1.653 0.028 0.163 3 3 M2-EVs-anti miR-342-3p 0.803 0.823 0.079 0.084 3 3
Figure3F-2-1 E-cadherin Control 0.924 0.751 0.854 M2-EVs-antiNC 0.242 0.254 0.296 M2-EVs-anti miR-342-3p 0.834 0.862 0.713
Figure3F-2-2 Vimentin Control 0.732 0.692 0.832 M2-EVs-antiNC 1.793 1.692 1.474 M2-EVs-anti miR-342-3p 0.756 0.795 0.917
Figure4F NEDD4L-WT NEDD4L-MUT mimic NC 1.01 0.943 0.09 0.092 3 3 miR-342-3p mimic 0.278 0.956 0.031 0.096 3 3
Figure4F-1 NEDD4L-WT mimic NC 1 1.105 0.925 miR-342-3p mimic 0.25 0.311 0.273
Figure4F-2 NEDD4L-MUT mimic NC 1.049 0.891 0.889 miR-342-3p mimic 0.876 0.93 1.062
Figure4G-1 NEDD4L CEP55 mimic NC 0.874 0.674 0.088 0.065 3 3 miR-342-3p mimic 0.214 1.321 0.024 0.126 3 3 inhibitor NC 0.845 0.743 0.083 0.075 3 3 miR-342-3p inhibitor 1.784 0.164 0.169 0.022 3 3
Figure4G-1-1 NEDD4L mimic NC 0.971 0.851 0.8 miR-342-3p mimic 0.191 0.239 0.212 inhibitor NC 0.775 0.823 0.937 miR-342-3p inhibitor 1.949 1.611 1.792
Figure4G-1-2 CEP55 mimic NC 0.616 0.662 0.744 miR-342-3p mimic 1.425 1.357 1.181 inhibitor NC 0.822 0.734 0.673 miR-342-3p inhibitor 0.189 0.148 0.155
Figure4G-2 NEDD4L CEP55 mimic NC 1.546 1.204 0.143 0.117 3 3 miR-342-3p mimic 0.723 2.546 0.074 0.243 3 3 inhibitor NC 1.453 1.343 0.142 0.132 3 3 miR-342-3p inhibitor 3.143 0.543 0.294 0.055 3 3
Figure4G-2-1 NEDD4L mimic NC 1.689 1.403 1.547 miR-342-3p mimic 0.718 0.799 0.652 inhibitor NC 1.309 1.593 1.456 miR-342-3p inhibitor 2.804 3.31 3.315
Figure4G-2-2 CEP55 mimic NC 1.071 1.252 1.29 miR-342-3p mimic 2.802 2.517 2.319 inhibitor NC 1.406 1.432 1.191 miR-342-3p inhibitor 0.5 0.524 0.605
Figure4I-1 NEDD4L CEP55 Control 0.784 0.342 0.082 0.032 3 3 M2-EVs-antiNC 0.187 0.894 0.023 0.092 3 3 M2-EVs-anti miR-342-3p 0.734 0.374 0.075 0.039 3 3
Figure4I-1-1 NEDD4L Control 0.87 0.707 0.774 M2-EVs-antiNC 0.167 0.181 0.212 M2-EVs-anti miR-342-3p 0.744 0.804 0.655
Figure4I-1-2 CEP55 Control 0.31 0.342 0.374 M2-EVs-antiNC 0.816 0.87 0.996 M2-EVs-anti miR-342-3p 0.364 0.417 0.341
Figure4I-2 NEDD4L CEP55 Control 1.435 0.674 0.137 0.073 3 3 M2-EVs-antiNC 0.453 1.343 0.046 0.137 3 3 M2-EVs-anti miR-342-3p 1.305 0.734 0.125 0.075 3 3
Figure4I-2-1 NEDD4L Control 1.464 1.555 1.286 M2-EVs-antiNC 0.497 0.405 0.457 M2-EVs-anti miR-342-3p 1.161 1.387 1.367
Figure4I-2-2 CEP55 Control 0.643 0.757 0.622 M2-EVs-antiNC 1.501 1.262 1.266 M2-EVs-anti miR-342-3p 0.72 0.815 0.667
Figure5A-1 NEDD4L CEP55 oe-NC 0.214 1.546 0.023 0.147 3 3 oe-NEDD4L 0.875 0.343 0.089 0.036 3 3 oe-NEDD4L + M2-EVs 0.287 1.454 0.031 0.147 3 3
Figure5A-1-1 NEDD4L oe-NC 0.212 0.238 0.192 oe-NEDD4L 0.795 0.86 0.971 oe-NEDD4L + M2-EVs 0.302 0.251 0.308
Figure5A-1-2 CEP55 oe-NC 1.551 1.691 1.397 oe-NEDD4L 0.335 0.382 0.311 oe-NEDD4L + M2-EVs 1.621 1.395 1.346
Figure5A-2 NEDD4L CEP55 oe-NC 0.767 0.893 0.082 0.085 3 3 oe-NEDD4L 1.674 0.276 0.155 0.028 3 3 oe-NEDD4L + M2-EVs 0.813 0.821 0.084 0.083 3 3
Figure5A-2-1 NEDD4L oe-NC 0.8 0.827 0.674 oe-NEDD4L 1.757 1.77 1.495 oe-NEDD4L + M2-EVs 0.739 0.904 0.795
Figure5A-2-2 CEP55 oe-NC 0.834 0.854 0.99 oe-NEDD4L 0.246 0.281 0.301 oe-NEDD4L + M2-EVs 0.8 0.912 0.75
Figure5B-1 0 24 48 72 96 oe-NC 0 0.613 1.231 1.612 1.765 0 0.063 0.125 0.158 0.175 3 3 3 3 3 oe-NEDD4L 0 0.231 0.556 0.789 0.904 0 0.022 0.053 0.081 0.088 3 3 3 3 3 oe-NEDD4L + M2-EVs 0 0.534 1.113 1.503 1.643 0 0.052 0.108 0.147 0.166 3 3 3 3 3
Figure5B-2 0 24 48 72 96 oe-NC 0 0.632 1.243 1.656 1.812 0 0.064 0.125 0.167 0.178 3 3 3 3 3 oe-NEDD4L 0 0.201 0.412 0.665 0.754 0 0.018 0.043 0.067 0.077 3 3 3 3 3 oe-NEDD4L + M2-EVs 0 0.542 1.113 1.512 1.674 0 0.051 0.105 0.148 0.164 3 3 3 3 3
Figure5C ACHN 769-P oe-NC 54.33 67.33 5.69 6.66 3 3 oe-NEDD4L 14.33 24.67 1.53 2.08 3 3 oe-NEDD4L + M2-EVs 46.33 58.33 4.62 6.11 3 3
Figure5C-1 ACHN oe-NC 56 59 48 oe-NEDD4L 14 16 13 oe-NEDD4L + M2-EVs 41 49 49
Figure5C-2 769-P oe-NC 69 73 60 oe-NEDD4L 23 24 27 oe-NEDD4L + M2-EVs 53 57 65
Figure5D ACHN 769-P oe-NC 20.33 25.33 2.08 2.89 3 3 oe-NEDD4L 7.67 12 0.58 1 3 3 oe-NEDD4L + M2-EVs 18.33 23.33 1.53 2.52 3 3
Figure5D-1 ACHN oe-NC 18 21 22 oe-NEDD4L 8 7 8 oe-NEDD4L + M2-EVs 20 18 17
Figure5D-2 769-P oe-NC 27 22 27 oe-NEDD4L 13 12 11 oe-NEDD4L + M2-EVs 21 26 23
Figure5E-1 E-cadherin Vimentin oe-NC 0.465 0.876 0.047 0.088 3 3 oe-NEDD4L 1.365 0.231 0.134 0.025 3 3 oe-NEDD4L + M2-EVs 0.523 0.833 0.055 0.085 3 3
Figure5E-1-1 E-cadherin oe-NC 0.443 0.433 0.519 oe-NEDD4L 1.44 1.444 1.21 oe-NEDD4L + M2-EVs 0.488 0.495 0.586
Figure5E-1-2 Vimentin oe-NC 0.799 0.858 0.972 oe-NEDD4L 0.207 0.257 0.23 oe-NEDD4L + M2-EVs 0.749 0.919 0.832
Figure5E-2 E-cadherin Vimentin oe-NC 0.743 1.521 0.072 0.146 3 3 oe-NEDD4L 1.674 0.403 0.164 0.042 3 3 oe-NEDD4L + M2-EVs 0.784 1.462 0.081 0.145 3 3
Figure5E-2-1 E-cadherin oe-NC 0.826 0.702 0.701 oe-NEDD4L 1.683 1.834 1.506 oe-NEDD4L + M2-EVs 0.877 0.731 0.744
Figure5E-2-2 Vimentin oe-NC 1.434 1.439 1.69 oe-NEDD4L 0.409 0.442 0.358 oe-NEDD4L + M2-EVs 1.521 1.297 1.568
Figure6B 7 14 21 28 35 Control 0 267.98 568.35 765.35 923.24 0 28.13 57.11 77.03 90.43 8 8 8 8 8 M2-EVs 0 495.22 953.24 1241.11 1432.13 0 45.23 92.13 117.13 136.35 8 8 8 8 8 oe-NEDD4L 0 102.13 242.23 365.35 432.24 0 10.03 23.43 37.21 44.24 8 8 8 8 8 oe-NEDD4L + M2-EVs 0 286.46 599.03 794.35 965.43 0 30.05 60.12 78.04 95.33 8 8 8 8 8 M2-EVs-anti miR-342-3p 0 231.33 538.34 742.24 887.64 0 21.55 52.11 72.13 89.44 8 8 8 8 8
Figure6C Control 0.74 0.63 0.65 0.72 0.63 0.77 0.66 0.56 M2-EVs 1.44 1.74 1.66 1.62 1.27 1.68 1.44 1.63 oe-NEDD4L 0.32 0.24 0.24 0.22 0.26 0.24 0.23 0.25 oe-NEDD4L + M2-EVs 0.68 0.82 0.74 0.73 0.71 0.61 0.8 0.66 M2-EVs-anti miR-342-3p 0.78 0.8 0.72 0.68 0.79 0.92 0.73 0.67
Figure6F Control 5 6 5 5 6 5 6 6 M2-EVs 13 15 18 16 18 16 18 16 oe-NEDD4L 2 2 1 2 2 2 2 2 oe-NEDD4L + M2-EVs 5 6 6 6 7 5 7 6 M2-EVs-anti miR-342-3p 6 6 6 6 6 7 6 5
Figure6H Control 1 1.069 0.854 1.092 1.086 1.035 1.061 0.749 M2-EVs 2.169 2.113 1.671 1.889 2.034 2.081 1.97 1.617 oe-NEDD4L 0.852 0.88 0.817 0.843 0.885 0.811 1.036 1.037 oe-NEDD4L + M2-EVs 2.669 2.6 2.174 2.555 2.46 2.623 2.401 3.031 M2-EVs-anti miR-342-3p 0.876 0.719 0.832 0.868 1.01 0.911 0.931 0.923
Figure6I CEP55 NEDD4L Control 0.874 1.267 0.085 0.125 8 8 M2-EVs 1.784 0.354 0.169 0.037 8 8 oe-NEDD4L 0.254 2.132 0.026 0.221 8 8 oe-NEDD4L + M2-EVs 0.865 1.287 0.089 0.132 8 8 M2-EVs-anti miR-342-3p 0.893 1.311 0.087 0.127 8 8
Figure6I-1 CEP55 Control 0.836 0.814 1.051 0.804 0.92 0.806 0.846 0.915 M2-EVs 1.751 1.485 1.847 1.869 1.865 2.031 1.611 1.813 oe-NEDD4L 0.242 0.269 0.233 0.245 0.313 0.245 0.24 0.244 oe-NEDD4L + M2-EVs 0.738 0.953 0.919 0.838 0.968 0.764 0.811 0.93 M2-EVs-anti miR-342-3p 0.939 0.904 0.788 0.911 0.777 0.913 1.05 0.862
Figure6I-2 NEDD4L Control 1.463 1.417 1.198 1.215 1.242 1.091 1.187 1.323 M2-EVs 0.412 0.341 0.334 0.357 0.388 0.376 0.296 0.328 oe-NEDD4L 2.245 2.217 2.051 2.509 1.802 1.939 2.031 2.261 oe-NEDD4L + M2-EVs 1.103 1.277 1.53 1.29 1.249 1.398 1.286 1.163 M2-EVs-anti miR-342-3p 1.359 1.489 1.259 1.326 1.279 1.227 1.094 1.455
Figure7A 0 0.5 1 2 DMSO 1.035 0.856 0.704 0.604 0.062 0.083 0.068 0.061 3 3 3 3 CHX 0.979 0.512 0.302 0.187 0.077 0.048 0.026 0.022 3 3 3 3 CHX + MG132 0.979 0.812 0.678 0.554 0.102 0.083 0.068 0.053 3 3 3 3
Figure7B oe-NC 0.765 0.809 0.661 oe-Smurf1 0.782 0.713 0.861 oe-Smurf2 0.722 0.881 0.833 oe-Itch 0.836 0.69 0.794 oe-NEDD4L 0.159 0.202 0.189
Figure7C oe-NC + DMSO 1.457 1.309 1.206 oe-NEDD4L + DMSO 0.39 0.318 0.353 oe-NC + MG132 1.307 1.152 1.402 oe-NEDD4L + MG132 1.211 1.461 1.423
Figure7G-1 NEDD4L CEP55 PI3K p-AKT/AKT p-mTOR/mTOR sh-NC 1.321 0.854 1.453 1.077 1.623 0.129 0.085 0.092 0.092 0.141 3 3 3 3 3 sh-NEDD4L 1.276 0.214 0.452 0.301 0.336 0.123 0.023 0.047 0.065 0.044 3 3 3 3 3 sh-NEDD4L + M2-EVs 0.364 0.798 1.324 0.949 1.474 0.038 0.081 0.136 0.106 0.253 3 3 3 3 3
Figure7G-1-1 NEDD4L sh-NC 1.451 1.193 1.32 sh-CEP55 1.367 1.325 1.136 sh-CEP55 + M2-EVs 0.364 0.326 0.402
Figure7G-1-2 CEP55 sh-NC 0.789 0.95 0.823 sh-CEP55 0.232 0.222 0.188 sh-CEP55 + M2-EVs 0.867 0.819 0.709
Figure7G-1-3 PI3K sh-NC 1.529 1.532 1.298 sh-CEP55 0.455 0.497 0.403 sh-CEP55 + M2-EVs 1.481 1.24 1.251
Figure7G-1-4 p-AKT/AKT sh-NC 1.086 0.98 1.164 sh-CEP55 0.376 0.271 0.257 sh-CEP55 + M2-EVs 0.898 1.071 0.878
Figure7G-1-5 p-mTOR/mTOR sh-NC 1.463 1.734 1.671 sh-CEP55 0.287 0.374 0.347 sh-CEP55 + M2-EVs 1.674 1.19 1.558
Figure7G-2 NEDD4L CEP55 PI3K/ p-AKT/AKT p-mTOR/mTOR sh-NC 0.813 1.543 0.874 1.644 1.126 0.083 0.145 0.085 0.306 0.169 3 3 3 3 3 sh-CEP55 0.845 0.453 0.215 0.501 0.256 0.083 0.047 0.021 0.068 0.036 3 3 3 3 3 sh-CEP55 + M2-EVs 0.284 1.453 0.842 1.76 1.011 0.031 0.138 0.082 0.227 0.199 3 3 3 3 3
Figure7G-2-1 NEDD4L sh-NC 0.864 0.717 0.858 sh-CEP55 0.775 0.824 0.936 sh-CEP55 + M2-EVs 0.251 0.288 0.313
Figure7G-2-2 CEP55 sh-NC 1.624 1.376 1.63 sh-CEP55 0.402 0.495 0.462 sh-CEP55 + M2-EVs 1.335 1.419 1.605
Figure7G-2-3 PI3K sh-NC 0.972 0.825 0.825 sh-CEP55 0.237 0.213 0.195 sh-CEP55 + M2-EVs 0.913 0.861 0.752
Figure7G-2-4 p-AKT/AKT sh-NC 1.384 1.566 1.981 sh-CEP55 0.561 0.427 0.516 sh-CEP55 + M2-EVs 1.802 1.515 1.962
Figure7G-2-5 p-mTOR/mTOR sh-NC 1.287 0.95 1.142 sh-CEP55 0.241 0.23 0.297 sh-CEP55 + M2-EVs 1.223 0.829 0.981