{"text_format":"3","workbook":"xls","n_sheets":1,"sheets":["Sheet1"],"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Sheet1","index":0,"n_rows":119,"n_cols":14,"rows_kept":119,"flags":["formulas_cached_only"]}
["Symbol","Name","HGNC_ID","Evidence","Test method","Test in","Test setting","Pathway","Confidence","Caution","Protein encoded","UniProtKB","PMID","Remark"]
["HMGB1","High mobility group box 1","HGNC:4983","Required for erastin-induced ferroptosis. Knockdown of HMGB1 decreased erastin-induced cell death.","Gene transfection, RNAi, cell viability assay, ELISA, LDH assay, flow cytometry, qPCR, western blot, electron microscopy","Human, mice","HL-60 cell, male NOD/SCID mice","HMGB1 :+: Ferroptosis","Validated","_NA_","High mobility group protein B1","P09429 (HMGB1_HUMAN)",31105999,"_NA_"]
["HMOX1","Heme oxygenase 1","HGNC:5013","Zinc protoporphyrin IX, a HO-1 inhibitor, prevented Erastin-triggered ferroptotic cancer cell death. Overexpression accelerates erastin-induced cell death.","Cell viability assay, qRT-PCR, western blot","Human, mice","HT-1080 fibrosarcoma cells, mice lung fibroblastic cells","HMOX1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Other articles (PMID 26403645, 28515173) suggest it a suppressor.","Heme oxygenase 1","P09601 (HMOX1_HUMAN)",26405158,"HO-1 in article"]
["HMOX1","Heme oxygenase 1","HGNC:5013","Inhibiting HO-1 effectively attenuated BAY-induced ferroptotic cell death. Defective HO-1 expression significantly rescued cell survival suppressed by BAY.","Cell viability assay, qRT-PCR, shRNA, gene overexpression, western blot","Human","MDA-MB-231, MDA-MB-468","HMOX1 :+: Ferroptosis","Validated","_NA_","Heme oxygenase 1","P09601 (HMOX1_HUMAN)",29274359,"HO-1 in article"]
["HMOX1","Heme oxygenase 1","HGNC:5013","Enhances the ferroptotic process in PRDX6-silenced cells by promoting cellular accumulation of ferrous ions. Overexpression increases both erastin and RSL-3-induced lipid ROS.","Gene transfection, western blot, qRT-PCR, LOOH quantification","Human","H1299 cells","HMOX1 :+: Ferroptosis","Validated","_NA_","Heme oxygenase 1","P09601 (HMOX1_HUMAN)",31036877,"_NA_"]
["HMOX1","Heme oxygenase 1","HGNC:5013","Knockdown of p62, quinone oxidoreductase‐1, heme oxygenase‐1, and ferritin heavy chain‐1 by RNA interference in HCC cells promoted ferroptosis in response to erastin and sorafenib.","qRT‐PCR, RNA interference","Human, mice","HepG2, Hepa1‐6, Hep3B, and SNU‐182 cells","HMOX1 :-: Ferroptosis","Validated","Article (PMID 26405158) suggests it a driver.","Heme oxygenase 1","P09601 (HMOX1_HUMAN)",26403645,"HO1 in article"]
["HMOX1","Heme oxygenase 1","HGNC:5013","Demonsrates antiferroptotic role. HO-1 deficiency promotes erastin-induced ferroptosis.","RT-PCR, western blot, cell viability, siRNA","Mice","Renal proximal tubule cells","HMOX1 :-: Ferroptosis","Validated","Article (PMID 26405158) suggests it a driver.","Heme oxygenase 1","P09601 (HMOX1_HUMAN)",28515173,"HO-1 in article"]
["HSPA5","Heat shock protein family A (Hsp70) member 5","HGNC:5238","Negatively regulates ferroptosis. Suppression of HSPA5 expression increased erastin-induced death. Overexpressed HSPA5 inhibited erastin-induced ferroptotic cell death.","Cell viability assay, western blot, shRNA, gene transfection","Human, mice","Pancreatic ductal adenocarcinoma cell lines (PANC1, CFPAC1, MiaPaCa2), nude mice, B6 mice","HSPA5 :+: GPX4, GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Endoplasmic reticulum chaperone BiP","P11021 (BIP_HUMAN)",28130223,"_NA_"]
["HSPA5","Heat shock protein family A (Hsp70) member 5","HGNC:5238","Knockdown of GRP78 enhanced artesunate-induced ferroptosis of pancreatic cancer cells.","Fluorescent staining, CCK8, Western blot, shRNA, qRT-PCR","Human, mice","Pancreatic cancer cells AsPC-1 and PaTU8988, BALB/c nude mice","GRP78 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Endoplasmic reticulum chaperone BiP","P11021 (BIP_HUMAN)",31456633,"GRP78 in article"]
["HSPA5","Heat shock protein family A (Hsp70) member 5","HGNC:5238","Serves as a negative regulator of DHA-induced ferroptosis.","Cell viability, colony formation, cell death, lipid ROS assay, GSH determination, siRNA, western blot, RT-PCR, xenograft tumor model","Human, mice","Glioma cell lines U251 and U373, mouse hippocampal neuronal cell line HT22, athymic nude BALB/c mice","HSPA5 :+: GPX4, GPX4 :-: Ferroptosis","Predicted","_NA_","Endoplasmic reticulum chaperone BiP","P11021 (BIP_HUMAN)",31519193,"_NA_"]
["HSPB1","Heat shock protein family B (small) member 1","HGNC:5246","Knockdown of HSF1 and HSPB1 enhances erastin-induced ferroptosis, whereas heat shock pretreatment and overexpression of HSPB1 inhibits erastin-induced ferroptosis.","RNAi, RT-qPCR, western blot, cell viability","Human, mice","HeLa and U2OS cells, human xenograft mouse tumor derived from HeLa (#CCL-2) cells.","HSPB1 :-: Ferroptosis","Validated","_NA_","Heat shock protein beta-1","P04792 (HSPB1_HUMAN)",25728673,"_NA_"]
["IREB2","Iron response element binding protein 2","HGNC:6115","Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis.","shRNA screening, qPCR, gene silencing","Human","NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell","IREB2 :+: Ferroptosis","Validated","_NA_","Iron-responsive element-binding protein 2","P48200 (IREB2_HUMAN)",22632970,"_NA_"]
["IREB2","iron responsive element binding protein 2","HGNC:6115","The gene targets of the enriched shRNAs are potential genes that positively regulate ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","IREB2 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","_NA_","Iron-responsive element-binding protein 2","P48200 (IREB2_HUMAN)",27514700,"_NA_"]
["KEAP1","Kelch like ECH associated protein 1","HGNC:23177","Knockdown of Keap1 reversed loss of p62-increased degradation of NRF2 in ferroptosis. Keap1 knockdown led to resistance to erastin-induced and sorafenib-induced growth inhibition with decreased ferroptotic events in the absence or presence of p62 knockdown.","Western blot, shRNA knockdown, cell viability","Human, mice","HepG2, Hepa1‐6, Hep3B, and SNU‐182 cells","KEAP1 :-: NFE2L2, NFE2L2 :-: Ferroptosis","Validated","_NA_","Kelch-like ECH-associated protein 1","Q14145 (KEAP1_HUMAN)",26403645,"_NA_"]
["KEAP1","Kelch like ECH associated protein 1","HGNC:23177","Keap 1 silencing decreased ferroptosis.","Cell viability assay, western blot, siRNA","Mice","Head and neck cancer cells, nude mice","KEAP1 :-: NFE2L2, NFE2L2 :-: Ferroptosis","Validated","_NA_","Kelch-like ECH-associated protein 1","Q14145 (KEAP1_HUMAN)",28012440,"_NA_"]
["KEAP1","Kelch like ECH associated protein 1","HGNC:23177","Keap1 inhibition promotes resistance to ferroptosis.","Cell viability analysis","Human, rat","F98, U87 glioma cells","KEAP1 :+: Ferroptosis","Validated","_NA_","Kelch-like ECH-associated protein 1","Q14145 (KEAP1_HUMAN)",28805788,"_NA_"]
["ACSF2","Acyl-CoA synthetase family member 2","HGNC:26101","Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis.","shRNA screening, qPCR, gene silencing","Human","NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell","ACSF2 :+: Ferroptosis","Validated","_NA_","Medium-chain acyl-CoA ligase ACSF2","Q96CM8 (ACSF2_HUMAN)",22632970,"_NA_"]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Deletion of this gene likely suppress ferroptosis by limiting the membrane-resident pool of oxidation-sensitive fatty acids.","Retroviral-mediated insertional mutagenesis and sequencing","Human","Chronic myeloid leukemia cell line KBM7","ACSL4 :+: Ferroptosis","Predicted","Author prediction based on gene function.","Long-chain-fatty-acid--CoA ligase 4","O60488 (ACSL4_HUMAN)",25965523,"_NA_"]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Knockdown inhibited erastin-induced ferroptosis, whereas overexpression restored ferroptosis sensitization.","shRNA, gene transfection, western blot","Human","LNCaP (human prostate cancer cells), K562 (human erythromyeloblastoid leukemia cell line), HepG2 (human hepatocellular cells), and HL60 (human promyelocytic leukemia cells)","ACSL4 :+: 5-HETE, 5-HETE :+: Ferroptosis","Validated","_NA_","Long-chain-fatty-acid--CoA ligase 4","O60488 (ACSL4_HUMAN)",27565726,"_NA_"]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Inhibition of ACSL4 was effective in protecting against RSL3-induced cell death. Acsl4 KO cells are resistant to ferroptosis.","Cell death count, LC-MS/MS,  fluorescence imaging","Mice","Mouse embryonic fibroblasts (Pfa1 cells)","ACSL4 :+: PE, PE :+: Ferroptosis","Validated","_NA_","Long-chain-fatty-acid--CoA ligase 4","O60488 (ACSL4_HUMAN)",27842066,"_NA_"]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","An essential proferroptotic gene. Re-expression of Flag-tagged human wild-type (WT) ACSL4 (ACSL4-Flag) in Acsl4 KO Pfa1 cells restored full sensitivity to ferroptosis induction. Inhibition showed significantly prolonged survival compared to vehicle-treated mice.","Cell viability, immunoblot,  lipid peroxidation assessment, CRISPR–Cas9, qPCR, microarray, RNA-seq","Human, mice","Mouse embryonic fibroblasts (Pfa1 cells), human breast cancer cell lines, Gpx4 KO mice","ACSL4 :+: Ferroptosis","Validated","_NA_","Long-chain-fatty-acid--CoA ligase 4","O60488 (ACSL4_HUMAN)",27842070,"_NA_"]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Knockout of Acsl4 in ferroptosis-sensitive cells conferred protection from erastin- and RSL3-induced cell death.","CRISPR/Cas9, western blotting, cell death assessment","Human, mice","NIH3T3, HT-29, HT-1080 and L929 cells","ACSL4 :+: Ferroptosis","Validated","_NA_","Long-chain-fatty-acid--CoA ligase 4","O60488 (ACSL4_HUMAN)",28551825,"_NA_"]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Inhibition suppresses ferroptosis.","siRNA, western blot, microscopy imaging, fluorescence microscopy, real-time PCR","Human, mice","Male C57BL/6 mice, Caco-2 cells","ACSL4 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Long-chain-fatty-acid--CoA ligase 4","O60488 (ACSL4_HUMAN)",30737476,"_NA_"]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Required for ferroptosis induced by erastin. ACSL4-null cells are resistant to ferroptosis induced by either erastin.","Western blot, cell death assay, CRISPR","Human","U2OS","ACSL4 :+: Ferroptosis","Validated","_NA_","Long-chain-fatty-acid--CoA ligase 4","O60488 (ACSL4_HUMAN)",30962574,"_NA_"]
["ACSL4","Acyl-CoA synthetase long chain family member 4","HGNC:3571","Overexpression induced ferroptosis. The opposite results were observed when ACSL4 was silenced.","Western blotting, immunofluorescence, 5‑HETE, LDH and CCK-8 cell viability assay","Human","HEB, T98G, U87 MG and U251 cells","ACSL4 :-: GPX4, GPX4 :-: Ferroptosis","Validated","_NA_","Long-chain-fatty-acid--CoA ligase 4","O60488 (ACSL4_HUMAN)",31789401,"_NA_"]
["AIFM2","Apoptosis inducing factor mitochondria associated 2","HGNC:21411","A glutathione-independent ferroptosis suppressor. Pharmacological targeting of FSP1 strongly synergizes with GPX4 inhibitors to trigger ferroptosis.","Expression cloning, cell death, cell viability, LDH assay, western blot, LC-MS, NADH consumption assay","Human, mice","MCF-7, HT1080, Pfa1 cells","FPS1 :+: CoQ10, CoQ10 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Apoptosis-inducing factor 2","Q9BRQ8 (AIFM2_HUMAN)",31634899,"FSP1 in article"]
["AIFM2","Apoptosis inducing factor mitochondria associated 2","HGNC:21411","A potent ferroptosis-resistance factor. Positively correlates with ferroptosis resistance.","Synthetic lethal CRISPR-Cas9 screen, cell death, western blotting, lipid droplet fractionation, glutathione measurement, tumour xenograft growth, CoQ measurement","Human, mice","U-2 OS cell line, NCI-H460, NCI-H2291, NCI-H1703 and NCIH446 cells, C.B17 SCID mice","FPS1 :+: CoQ10, CoQ10 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Apoptosis-inducing factor 2","Q9BRQ8 (AIFM2_HUMAN)",31634900,"FSP1 in article"]
["AKR1C1","Aldo-keto reductase family 1 member C1","HGNC:384","Up-regulated in DU-145 erastin-resistant clones. Participate in the detoxification of toxic lipid metabolites. May confer partial resistance to erastin by enhancing the detoxification of reactive aldehydes generated downstream of the oxidative destruction of the plasma membrane during ferroptosis.","RNA-seq, glutamate release assay, cell growth, flow cytometry","Human","DU-145 prostate cancer cells","AKR1C1 :-: Ferroptosis","Validated","_NA_","Aldo-keto reductase family 1 member C1","Q04828 (AK1C1_HUMAN)",24844246,"_NA_"]
["AKR1C1","Aldo-keto reductase family 1 member C1","HGNC:384","Inhibition completely resensitizes resistant melanoma cells to ferroptosis execution.","RNAi, cell transfection, western blotting, qRT-PCR, cell viability, lipid peroxides evaluation","Human","MeWo, A2058, SK-Mel 5, SK-Mel 24, C8161, CHL-1, and A375 cells","AKR1C1 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Aldo-keto reductase family 1 member C1","Q04828 (AK1C1_HUMAN)",31780644,"_NA_"]
["AKR1C2","Aldo-keto reductase family 1 member C2","HGNC:385","Up-regulated in DU-145 erastin-resistant clones. Participate in the detoxification of toxic lipid metabolites. May confer partial resistance to erastin by enhancing the detoxification of reactive aldehydes generated downstream of the oxidative destruction of the plasma membrane during ferroptosis.","RNA-seq, glutamate release assay, cell growth, flow cytometry","Human","DU-145 prostate cancer cells","AKR1C2 :-: Ferroptosis","Validated","_NA_","Aldo-keto reductase family 1 member C2","P52895 (AK1C2_HUMAN)",24844246,"_NA_"]
["AKR1C2","Aldo-keto reductase family 1 member C2","HGNC:385","Inhibition completely resensitizes resistant melanoma cells to ferroptosis execution.","RNAi, cell transfection, western blotting, qRT-PCR, cell viability, lipid peroxides evaluation","Human","MeWo, A2058, SK-Mel 5, SK-Mel 24, C8161, CHL-1, and A375 cells","AKR1C2 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Aldo-keto reductase family 1 member C2","P52895 (AK1C2_HUMAN)",31780644,"_NA_"]
["ALOX12","Arachidonate 12-lipoxygenase, 12S type","HGNC:429","Silencing ALOX genes made cells resistant to ferroptosis.","qPCR","Human","G-401 cells","ALOX12 :+: PUFAs peroxidation, PUFAs peroxidation :+: Ferroptosis","Validated","_NA_","Arachidonate 12-lipoxygenase","P18054 (LOX12_HUMAN)",27506793,"_NA_"]
["ALOX12","Arachidonate 12-lipoxygenase, 12S type","HGNC:429","12‐LOX inhibitors prevented cell death, whereas ALOX12 overexpression significantly enhanced cell death. ALOX12 expression was gradually elevated during the erastin or RSL3 treatments, and was stable in the late stage of ferroptosis.","siRNA, transfection, western blot, cellular lipoxygenase assay","Human","HT1080, Panc‐1, Calu‐1 cells","ALOX12 :+: Ferroptosis","Validated","_NA_","Arachidonate 12-lipoxygenase","P18054 (LOX12_HUMAN)",28837253,"_NA_"]
["ALOX12","Arachidonate 12-lipoxygenase, 12S type","HGNC:429","An essential factor of p53-dependent ferroptosis. Loss of one Alox12 allele is sufficient to abrogate p53-mediated ferroptosis.","RNAi, qRT-PCR, western blot, CRISPR, cell death assay, GSH measurement, flow cytometer","Human, mice","H1299 cells, U2OS cells, mouse embryonic fibroblasts, mice","ALOX12 :+: PUFAs-OOH, PUFAs-OOH :+: Ferroptosis","Validated","_NA_","Arachidonate 12-lipoxygenase","P18054 (LOX12_HUMAN)",30962574,"_NA_"]
["ALOX15","Arachidonate 15-lipoxygenase","HGNC:433","Silencing ALOX genes made cells resistant to ferroptosis.","qPCR","Human","G-401 cells","ALOX15 :+: PUFAs peroxidation, PUFAs peroxidation :+: Ferroptosis","Validated","_NA_","Arachidonate 15-lipoxygenase","P16050 (LOX15_HUMAN)",27506793,"_NA_"]
["ALOX15","Arachidonate 15-lipoxygenase","HGNC:433","SAT1- and ROS-induced ferroptosis was completely abrogated by PD146176, an ALOX15-specific inhibitor.","Cell death count, qRT-PCR, siRNA","Human","H1299, U2OS cells","ALOX15 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Arachidonate 15-lipoxygenase","P16050 (LOX15_HUMAN)",27698118,"_NA_"]
["ALOX15","Arachidonate 15-lipoxygenase","HGNC:433","Liproxstatin-1 inhibited the 15-LOX enzymatic activity and suppressed ferroptosis.","Identification of oxygenated PE","Mice","Kidney of GPX4-deficient mice","ALOX15 :+: PE, PE :+: Ferroptosis","Validated","_NA_","Arachidonate 15-lipoxygenase","P16050 (LOX15_HUMAN)",27842066,"15-LOX in article"]
["ALOX15","Arachidonate 15-lipoxygenase","HGNC:433","Suppression of ferroptosis following ALOX15 silencing was detected in cancer cells. Cells with exogenous expression of ALOX15 had an increased cell death rate following RSL3 treatment.","siRNA, transfection, western blot, cellular lipoxygenase assay","Human","HT1080, Panc‐1, Calu‐1 cells","ALOX15 :+: Ferroptosis","Validated","_NA_","Arachidonate 15-lipoxygenase","P16050 (LOX15_HUMAN)",28837253,"_NA_"]
["ATF3","Activating transcription factor 3","HGNC:785","Promotes ferroptosis induced by erastin.","Cell viability assay, transfection, lentiviral infection, qRT-PCR, western blot","Human","HT1080 cells, retinal pigment epithelial cells","ATF3 :-: SLC7A11, SLC7A11 :+: System Xc-, System Xc- :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Cyclic AMP-dependent transcription factor ATF-3","P18847 (ATF3_HUMAN)",31273299,"_NA_"]
["ATF4","Activating transcription factor 4","HGNC:786","Inhibition of ATF4 expression increased erastin-induced cell death. ATF4 results in the induction of HSPA5, which in turn protects against GPX4 protein degradation and subsequent ferroptosis.","CCK8 cell viability assay, clonogenic cell survival assay, western blot, q-PCR, RNAi","Human","Pancreatic ductal adenocarcinoma cells (e.g., PANC1, CFPAC1, MiaPaCa2, and Panc2.03)","ATF4 :+: HSPA5, HSPA5 :+: GPX4, GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Cyclic AMP-dependent transcription factor ATF-4","P18848 (ATF4_HUMAN)",28130223,"_NA_"]
["ATF4","Activating transcription factor 4","HGNC:786","ATF4 expression induces acquired cell death resistance. ATF4 knockdown renders cells susceptible for ferroptosis.","siRNA, cell transfection, cell viability assay, RT-PCR, immunoblotting","Human","U87 and U251 glioblastoma cells","ATF4 :+: xCT, xCT :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Cyclic AMP-dependent transcription factor ATF-4","P18848 (ATF4_HUMAN)",28553953,"_NA_"]
["ATG5","Autophagy related 5","HGNC:589","Knockout or knockdown limited erastin-induced ferroptosis.","shRNA","Human, mice","Mouse embryonic fibroblasts, human pancreatic cancer cell lines (PANC1 and PANC2.03), and the human fibrosarcoma cell line HT-1080","ATG5 :+: Ferroptosis","Validated","_NA_","Autophagy protein 5","Q9H1Y0 (ATG5_HUMAN)",27245739,"_NA_"]
["ATG5","Autophagy related 5","HGNC:589","Potential positive regulators of ferroptosis. Knockout of ATG5 led to significantly lower levels of erastin-induced ferroptosis in a dose- and time-dependent manner.","RNAi screening","Mice","Mouse embryonic fibroblasts","ATG5 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Autophagy protein 5","Q9H1Y0 (ATG5_HUMAN)",27514700,"_NA_"]
["ATP5MC3","ATP synthase membrane subunit c locus 3","HGNC:843","Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis.","shRNA screening, qPCR, gene silencing","Human","NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell","ATP5MC3 :+: Ferroptosis","Validated","_NA_","ATP synthase F(0) complex subunit C3","P48201 (AT5G3_HUMAN)",22632970,"ATP5G3 in article"]
["BECN1","Beclin 1","HGNC:1034","Potential positive regulators of ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","BECN1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","_NA_","Beclin-1","Q14457 (BECN1_HUMAN)",27514700,"_NA_"]
["BECN1","Beclin 1","HGNC:1034","Knockdown inhibits ferroptosis. Overexpression increases ferroptotic cancer cell death.","shRNA, western blot, cell viability assay, gene overexpression","Human, mice","HCT116, CX-1, PANC1, HT1080, and Calu-1 cells, athymic nude immunodeficient mice","BECN1 :-: System Xc-, System Xc- :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Beclin-1","Q14457 (BECN1_HUMAN)",30057310,"_NA_"]
["BECN1","Beclin 1","HGNC:1034","Overexpression aggravated isoflurane-induced cell damage by upregulating ferroptosis. This phenomenon was significantly attenuated by silencing of Beclin1.","Cell viability, RNAi, RT-qPCR, western blot, lipid peroxidation, iron, GSH and glutamate release assays","Human","SH-SY5Y cells","(Beclin1/SLC7A11) :-: System Xc-, System Xc- :-: Ferroptosis","Validated","_NA_","Beclin-1","Q14457 (BECN1_HUMAN)",31650158,"Beclin1 in article"]
["CBS","Cystathionine beta-synthase","HGNC:1550","Inhibition triggers ferroptosis in hepatocellular carcinoma.","Cell viability, western blotting, LC/ESI-MS/MS, flow cytometry, xenograft mice","Human, mice","HepG2 cells, female ICR mice","CBS :-: Ferroptosis","Validated","_NA_","Cystathionine beta-synthase","P35520 (CBS_HUMAN)",30258181,"_NA_"]
["CBS","Cystathionine beta-synthase","HGNC:1550","Knockdown in erastin-resistant cells caused ferroptotic cell death, while overexpression conferred ferroptosis resistance.","RNAi, cell viability, ROS analysis, GSH, glutamate release and cystine uptake assays, western blotting, qRT-PCR","Human","SKOV3 and OVCA429 cells","CBS :+: Cystathionine, Cystathionine :+: Cysteine, Cysteine :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Cystathionine beta-synthase","P35520 (CBS_HUMAN)",31819185,"_NA_"]
["CDKN1A","Cyclin dependent kinase inhibitor 1A","HGNC:1784","Required to to suppress ferroptosis.","CRISPR, shRNA, immunoblotting, cell-cycle profile, cell death quantification","Human","HT-1080 cells","CDKN1A :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Cyclin-dependent kinase inhibitor 1","P38936 (CDN1A_HUMAN)",29346757,"Encoding p21"]
["CHAC1","ChaC glutathione specific gamma-glutamylcyclotransferase 1","HGNC:28680","CHAC1 degradation of GSH might enhance cystine-starvation-induced cell death.","Western blot, real-time PCR, cell viability assay, siRNA, GSH assay kit","Human","MDA-MB-231, Hs 578T, and HCC 1937 cells","CHAC1 :-: GSH, GSH :-: Ferroptosis","Validated","_NA_","Glutathione-specific gamma-glutamylcyclotransferase 1","Q9BUX1 (CHAC1_HUMAN)",29383104,"_NA_"]
["DPP4","Dipeptidyl peptidase 4","HGNC:3009","Required for ferroptosis in TP53-deficient CRC cells.","Cell death, RNAi, western blot, enzyme activity","Human, mice","TP53-/- HCT116 cells, athymic nude mice","DPP4 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Dipeptidyl peptidase 4","P27487 (DPP4_HUMAN)",28813679,"_NA_"]
["CDO1","Cysteine dioxygenase type 1","HGNC:1795","CDO1 suppression contributes to ferroptosis resistance.","siRNA, western blot, MTT assay","Human","Gastric cancer cells AGS, BGC823, athymic nude mice","CDO1 :-: GPX4, GPX4 :-: Ferroptosis","Validated","_NA_","Cysteine dioxygenase type 1","Q16878 (CDO1_HUMAN)",29144989,"_NA_"]
["EIF2S1","Eukaryotic translation initiation factor 2 subunit 1","HGNC:3265","Phosphorylated in erastin-treated sample.","Western blot","Human","HT-1080 cells","Deduced","_NA_","Eukaryotic translation initiation factor 2 subunit 1","P05198 (IF2A_HUMAN)",24844246,"eIF2alpha in article. May promote ferroptosis."]
["FTH1","Ferritin heavy chain 1","HGNC:3976","Knockdown of p62, quinone oxidoreductase‐1, heme oxygenase‐1, and ferritin heavy chain‐1 by RNA interference in HCC cells promoted ferroptosis in response to erastin and sorafenib.","qRT‐PCR, RNA interference","Human, mice","HepG2, Hepa1‐6, Hep3B, and SNU‐182 cells","FTH1 :-: Ferroptosis","Validated","_NA_","Ferritin heavy chain","P02794 (FRIH_HUMAN)",26403645,"_NA_"]
["FTH1","Ferritin heavy chain 1","HGNC:3976","FTH reconstituted cells exhibited the reduced lipid peroxides content and restored the DHA-induced ferroptosis.","Cell viability, cell cycle, ROS determination, western blot, immunofluorescence, ectopic expressing, lentiviral transduction","Human","HL60, KG1, THP-1 cells","FTH1 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Ferritin heavy chain","P02794 (FRIH_HUMAN)",30557609,"FTH in article"]
["GLS2","Glutaminase 2","HGNC:29570","Both inhibitor inhibition and gene knockdown inhibit ferroptotic cell death.","shRNA, western blot, cell viability","Mice","Mouse embryonic fibroblasts","GSL2 :+: Glutamate, Glutamate :+: Ferroptosis","Validated","_NA_","Glutaminase liver isoform","Q9UI32 (GLSL_HUMAN)",26166707,"_NA_"]
["GLS2","Glutaminase 2","HGNC:29570","Upregulated in erastin-treated wild-type MEFs, and silencing Gls2 exihibits cell death defect in wild-type MEFs treated with erastin.","Cell viability assay,western blot","Mice","Mouse embryonic fibroblasts","GLS2 :+: Ferroptosis","Validated","_NA_","Glutaminase liver isoform","Q9UI32 (GLSL_HUMAN)",27034505,"_NA_"]
["GLS2","Glutaminase 2","HGNC:29570","The gene targets of the enriched shRNAs are potential genes that positively regulate ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","GLS2 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","_NA_","Glutaminase liver isoform","Q9UI32 (GLSL_HUMAN)",27514700,"_NA_"]
["GOT1","Glutamic-oxaloacetic transaminase 1","HGNC:4432","RNAi reduced ferroptosis.","shRNA, qPCR, cell viability","Mice","Mouse embryonic fibroblasts","GOT1 :+: alpha-Ketoglutarate, alpha-Ketoglutarate :+: Ferroptosis","Validated","_NA_","Aspartate aminotransferase","P17174 (AATC_HUMAN)",26166707,"_NA_"]
["GOT1","Glutamic-oxaloacetic transaminase 1","HGNC:4432","Overexpression of miR‐9 suppressed GOT1, which subsequently reduced ferroptosis. Overexpression of GOT1 restored miR‐9 mediated ferroptosis suppression.","Immunoblotting, luciferase reporter assay, qPCR, cell viability assay, MDA assay, iron assay, glutamate assay","Human","A375 and G‐361 cells","GOT1 :+: alpha KG, alpha KG :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Aspartate aminotransferase","P17174 (AATC_HUMAN)",30035324,"_NA_"]
["GPX2","Glutathione peroxidase 2","HGNC:4554","Expression was upregulated during ferroptosis induced by erastin or RSL3.","RT-qPCR, cell viability","Human","BJeLR cells","Deduced","Inferred as ferroptotic marker because of change (|delta ct| > 2) in gene expression.","Glutathione peroxidase 2","P18283 (GPX2_HUMAN)",24439385,"May promote ferroptosis."]
["GPX4","Glutathione peroxidase 4","HGNC:4556","RNAi-mediated GPX4 knockdown induces ferroptosis.","Affinity-based chemoproteomics, western blotting, and RNAi","Human, mice","HRAS mutant BJeLR-engineered tumor cells, HT-1080 cells, xenograft mouse model (athymic nude mice injected with four million HT-1080 cells)","GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Phospholipid hydroperoxide glutathione peroxidase","P36969 (GPX4_HUMAN)",24439385,"_NA_"]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Knockout of glutathione peroxidase 4 (Gpx4) causes cell death in a pathologically relevant form of ferroptosis. Knockdown renders cells more sensitive to ferroptosis-inducing agents.","Cell viability assessment, siRNA","Human, mice","Mouse embryonic fibroblast (MEF) cells (Pfa1 cells), Gpx4−/− cells and mice, HK-2 cells","GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Phospholipid hydroperoxide glutathione peroxidase","P36969 (GPX4_HUMAN)",25402683,"_NA_"]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Ex vivo, Gpx4-deficient T cells rapidly accumulated membrane lipid peroxides and concomitantly underwent cell death driven by ferroptosis.","Viability assays, flow cytometry, lipid peroxidation assay","Mice","T cells","GPX4 :-: Ferroptosis","Validated","_NA_","Phospholipid hydroperoxide glutathione peroxidase","P36969 (GPX4_HUMAN)",25824823,"_NA_"]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Knockdown increased cell death.","siRNA, western blot, cell death test","Human","MDA MB 231 and SKBr3 cells","GPX4 :-: Ferroptosis","Validated","_NA_","Phospholipid hydroperoxide glutathione peroxidase","P36969 (GPX4_HUMAN)",27441659,"_NA_"]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Protects lipid peroxidation. Cell damage induced by GPx4 ablation is involved in ferroptosis.","RT-PCR, western blot, cytotoxicity assay, cell proliferation assay, siRNA","Human","Umbilical vein endothelial cell","GPX4 :-: LDH, LDH :+: Ferroptosis","Validated","_NA_","Phospholipid hydroperoxide glutathione peroxidase","P36969 (GPX4_HUMAN)",27964880,"_NA_"]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Ferroptosis drives neurodegeneration in Gpx4BIKO mice.","RT-qPCR, behavior test, western blot","Mice","Forebrain neurons of Gpx4BIKO mice and Gpx4(f/f) mice","GPX4 :-: Ferroptosis","Validated","_NA_","Phospholipid hydroperoxide glutathione peroxidase","P36969 (GPX4_HUMAN)",28212525,"_NA_"]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Downregulation conferred increased sensitivity to ferroptosis following cystine deprivation.","Immunoblotting, cell viability","Human","Human mammary epithelial cells","GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Phospholipid hydroperoxide glutathione peroxidase","P36969 (GPX4_HUMAN)",28297659,"_NA_"]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Activation blocked ferroptosis.","Cell viability assay, lipid ROS measurement, RT-qPCR, western blot","Mice","TM4 Sertoli cells","GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Phospholipid hydroperoxide glutathione peroxidase","P36969 (GPX4_HUMAN)",29436589,"_NA_"]
["GPX4","Glutathione peroxidase 4","HGNC:4556","GPX4-overexpressing cells were resistant to reactive oxygen species-induced cell death. Conversely, GPX4-knockdown cells were sensitive to reactive oxygen species-induced cell death.","Immunohistochemistry, qRT-PCR,lentiviral transduction, shRNA, flow cytometry, cell viability assay","Human","LCL-K cell","GPX4 :-: 8-OHdG, 8-OHdG :+: Ferroptosis","Deduced","The authors did not determine wether the ROS-induced cell death is related to ferroptosis or not.","Phospholipid hydroperoxide glutathione peroxidase","P36969 (GPX4_HUMAN)",29463878,"_NA_"]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Overexpression GPX4 resulted in decreased cell death after RSL3 treatment. Moreover, this effect was able to be reversed by overexpression of GPX4.","Cell viability, cell death, ROS determination, western blot","Human","HCT116, LoVo, and HT29 CRC cells","GPX4 :-: Ferroptosis","Validated","_NA_","Phospholipid hydroperoxide glutathione peroxidase","P36969 (GPX4_HUMAN)",30524291,"_NA_"]
["GPX4","Glutathione peroxidase 4","HGNC:4556","Depletion or inhibition resulted in cell death by ferroptosis.","Quantitative proteomic analysis, siRNA, RNA-seq and qRT-PCR, cell viability, lipid peroxidation assay, GSH measurement, western blot","Human, mice, rat","H9c2 and C2C12 cells, rat ventricular myocytes, C57BL/6 mice","GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Phospholipid hydroperoxide glutathione peroxidase","P36969 (GPX4_HUMAN)",31685805,"_NA_"]
["HIF1A","Hypoxia inducible factor 1 subunit alpha","HGNC:4910","Re-sensitized HIF-2alpha-null cells to ferroptosis.  Induce ferroptosis sensitivity in cancer cells.","cDNA screenin, cell viability","Mice","786-O cells","HIF1A :+: HILPDA, HIF1A :+: PLIN2, HILPDA :+: PUFA, PLIN2 :+: PUFA, PUFA :+: PUFA-phospholipid hydroperoxides, PUFA-phospholipid hydroperoxides :+: Ferroptosis","Validated","_NA_","Hypoxia-inducible factor 1-alpha","Q16665 (HIF1A_HUMAN)",30962421,"HIF-1alpha in article"]
["HIF1A","Hypoxia inducible factor 1 subunit alpha","HGNC:4910","Destabilizing HIF1A facilitated ferroptotic tumor cell death.","Cytotoxicity assays, western blot, immunoprecipitation, RNA interference, gene transfection, qPCR, MDA assay","Human, mice","Calu-1, THP1, HT1080, and HL-60 cells, athymic nude mice","HIF1A  :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Hypoxia-inducible factor 1-alpha","Q16665 (HIF1A_HUMAN)",31355331,"_NA_"]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","NRF2 plays a central role in protecting hepatocellular carcinoma (HCC) cells against ferroptosis","Cell Viability Analysis, western blot, RNAi, qRT-PCR","Human, mice","HepG2, Hepa1‐6, Hep3B, and SNU‐182 cells, C57BL/6 mice","NFE2L2 :+: (NQO1/HMOX1/FTH1), (NQO1/HMOX1/FTH1) :-: Ferroptosis","Validated","_NA_","Nuclear factor erythroid 2-related factor 2","Q16236 (NF2L2_HUMAN)",26403645,"NRF2 in article"]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Required for sorafenib‐induced expression of MT‐1G which is a ferroptosis suppressor.","shRNA, qRT-PCR, western blot","Human","Huh7 and/or HepG2 cells","NFE2L2 :+: MT1G, MT1G :-: Ferroptosis","Deduced","Its ability to induce MT1G was validated, but its role in ferroptosis needs validation.","Nuclear factor erythroid 2-related factor 2","Q16236 (NF2L2_HUMAN)",27015352,"NRF2 in article"]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Nrf2 activation contributes to the resistance of HNCs to artesunate-induced ferroptosis. Nrf2 inhibition sensitizes head and neck cancer cells to artesunate-induced ferroptosis.","Cell viability assay, RNAi, gene transfection, western blot","Mice","Head and neck cancer cells (HN2–10), nude mice","NFE2L2 :-: Ferroptosis","Validated","_NA_","Nuclear factor erythroid 2-related factor 2","Q16236 (NF2L2_HUMAN)",28012440,"NRF2 in article"]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Nrf2 over expression promotes resistance to ferroptosis.","Cell growth assay","Human, rat","F98, U87 glioma cells","NFE2L2 :+: xCT, xCT :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Nuclear factor erythroid 2-related factor 2","Q16236 (NF2L2_HUMAN)",28805788,"Nrf2 in article"]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","ARF-mediated ferroptosis was largely abrogated by co-expression of NRF2.","Gene transfection, cell death quantification","Human, mice","H1299, SaoS2, U2OS cells, nude mice","NFE2L2 :+: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","_NA_","Nuclear factor erythroid 2-related factor 2","Q16236 (NF2L2_HUMAN)",28985506,"NRF2 in article"]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Associated with resistance to ferroptosis. Inhibition of Nrf2 sensitized cells to ferroptosis.","Cell viability and cell death assays, ROS production measurement, RNAi, gene transfection, immunoblotting","Human, mice","HNC cell line (AMC-HN2–11), SNUcell lines (SNU-1041, -1066, and -1076), athymic BALB/c male nude mice","NFE2L2 :+: FTH1, FTH1 :-: Ferroptosis, NFE2L2 :+: FPN, FPN :-: Ferroptosis, NFE2L2 :+: HO-1, HO-1 :-: Ferroptosis","Validated","_NA_","Nuclear factor erythroid 2-related factor 2","Q16236 (NF2L2_HUMAN)",30339884,"Nrf2 in article"]
["NFE2L2","Nuclear factor, erythroid 2 like 2","HGNC:7782","Genetically repression of NRF2 enhanced ferroptosis susceptibility.","RNAi, cell viability, ROS analysis, GSH, glutamate release and cystine uptake assays, western blotting, qRT-PCR","Human","SKOV3 and OVCA429 cells","NFE2L2 :+: CBS :+: Cystathionine, Cystathionine :+: Cysteine, Cysteine :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Nuclear factor erythroid 2-related factor 2","Q16236 (NF2L2_HUMAN)",31819185,"NRF2 in article"]
["NOX4","Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) 4","HGNC:7891","Suppresses erastin-induced ferroptosis when inhibited by inhibitor.","Inhibition test by diphenylene iodonium (DPI) and GKT137831.","Human","KRAS mutant Calu-1 non small cell lung cancer cell","NOX4 :+: Ferroptosis","Deduced","The presented inhibition study is not sufficient to confirm its role in ferroptosis.","NADPH oxidase 4","Q9NPH5 (NOX4_HUMAN)",22632970,"_NA_"]
["NOX4","NADPH oxidase 4","HGNC:7891","Inhibition of NADPH oxidase 4 (NOX4) blocked ferroptosis.","Fluorescence-activated cell sorting (FACS) analysis, cell viability","Human","Human mammary epithelial) cells","NOX4 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","NADPH oxidase 4","Q9NPH5 (NOX4_HUMAN)",28297659,"_NA_"]
["NOX4","NADPH oxidase 4","HGNC:7891","Activated  Nox4  contributes to  PAB-induced  ferroptotic cell death. knockdown made cells resistant to PAB-induced death.","Western blot, LDH release assay, siRNA, cell death, siRNA","Human, rat","Rat C6, human SHG-44, U87 and U251 glioma cells","NOX4 :+: H2O2, H2O2 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","NADPH oxidase 4","Q9NPH5 (NOX4_HUMAN)",29702192,"_NA_"]
["NOX4","NADPH oxidase 4","HGNC:7891","Knockdown inhibited ferroptosis.","Immunohistochemistry, western blot, cell death assay, siRNA","Rat","Sprague Dawley rats","NOX4 :+: Ferroptosis","Validated","_NA_","NADPH oxidase 4","Q9NPH5 (NOX4_HUMAN)",31196626,"_NA_"]
["SCD","Stearoyl-CoA desaturase","HGNC:10571","Depletion of the SCD1 and FADS2 metabolic genes induces ferroptosis.","shRNA, RT-qPCR, MTT assay, total iron detection, lipid ROS detection","Human","A549 cancer cells","SCD :-: Ferroptosis","Validated","_NA_","Acyl-CoA desaturase","O00767 (ACOD_HUMAN)",28900510,"SCD1 in article"]
["SCD","Stearoyl-CoA desaturase","HGNC:10571","Inhibition of SCD1 induces ferroptotic cell death. Expression of SCD1 protects cells from ferroptosis.","Cell viability, cell death, BODIPY staining, siRNA, RT-qPCR, western blot, gene overexpression, ","Human, mice","OVCAR-4, COV362, FT-t cells, mouse orthotopic xenograft model","SCD :+: CoQ10, CoQ10 :-: Ferroptosis","Validated","_NA_","Acyl-CoA desaturase","O00767 (ACOD_HUMAN)",31270077,"SCD1 in article"]
["SLC1A5","Solute carrier family 1 member 5","HGNC:10943","Pharmacological inhibition by L-g-glutamyl-p-nitroanilide or RNAi knockdown markedly blocked ferroptosis.","RNAi knockdown, cell survival analysis","Mice","Mouse embryonic fibroblasts","SLC1A5 :+: Glutamine, Glutamine :+: Glutamate, Glutamate :+: alpha-Ketoglutarate, alpha-Ketoglutarate :+: Ferroptosis","Validated","_NA_","Neutral amino acid transporter B(0)","Q15758 (AAAT_HUMAN)",26166707,"_NA_"]
["SLC1A5","Solute carrier family 1 member 5","HGNC:10943","Overexpression of SLC1A5 restored miR-137-mediated ferroptosis suppression.","Cell viability assay, lipid ROS assay, western blot, gene overexpression, shRNA","Human","Melanoma cell lines A375 and G-361","SLC1A5 :+: Glutaminolysis, Glutaminolysis :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Neutral amino acid transporter B(0)","Q15758 (AAAT_HUMAN)",29348676,"_NA_"]
["SLC38A1","Solute carrier family 38 member 1","HGNC:13447","RNAi knockdown markedly blocked ferroptosis.","RNAi knockdown, cell survival analysis","Mice","Mouse embryonic fibroblasts","SLC38A1 :+: Glutamine, Glutamine :+: Glutamate, Glutamate :+: alpha-Ketoglutarate, alpha-Ketoglutarate :+: Ferroptosis","Validated","_NA_","Sodium-coupled neutral amino acid transporter 1","Q9H2H9 (S38A1_HUMAN)",26166707,"_NA_"]
["SLC38A1","Solute carrier family 38 member 1","HGNC:13447","The gene targets of the enriched shRNAs are potential genes that positively regulate ferroptosis.","RNAi screening","Mice","Mouse embryonic fibroblasts","SLC38A1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Screened","_NA_","Sodium-coupled neutral amino acid transporter 1","Q9H2H9 (S38A1_HUMAN)",27514700,"_NA_"]
["SLC3A2","Solute carrier family 3 member 2","HGNC:11026","Required for in vitro cell survival because of its role in protecting cells from ferroptosis.","Cell survival test","Mice","Mouse ES-derived fibroblasts","SLC3A2 :+: System Xc-, System Xc- :-: Ferroptosis","Validated","_NA_","4F2 cell-surface antigen heavy chain","P08195 (4F2_HUMAN)",26945935,"CD98hc in article"]
["SLC40A1","Solute carrier family 40 member 1","HGNC:10909","Involved in siramesine and lapatinib-induced ferroptotic cell death. Its expression is decreased after treatment with siramesine alone or in combination with lapatinib. Overexpression FPN resulted in decreased ROS and cell death whereas knockdown of FPN increased cell death after siramesine and lapatinib treatment.","Western blot, densitometry quantification, siRNA, gene transfection, cell death test","Human","MDA MB 231 and SKBr3 cells","SLC40A1 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Solute carrier family 40 member 1","Q9NP59 (S40A1_HUMAN)",27441659,"Ferroportin-1 (FPN) in article"]
["SLC40A1","Solute carrier family 40 member 1","HGNC:10909","Overexpression of Fpn inhibited ferroptosis.","Cell viability assay, lipid ROS measurement, RT-qPCR, western blot","Mice","TM4 Sertoli cells","SLC40A1 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Solute carrier family 40 member 1","Q9NP59 (S40A1_HUMAN)",29436589,"Fpn in article"]
["SLC40A1","Solute carrier family 40 member 1","HGNC:10909","A negative regulator of ferroptosis by reducing intracellular iron concentration. Knockdown accelerates erastin-induced ferroptosis.","Cell viability, qRT-PCR, western blot, siRNA","Human","SH-SY5Y cells","SLC40A1 :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Solute carrier family 40 member 1","Q9NP59 (S40A1_HUMAN)",29949159,"Ferroportin in article"]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Silencing of SLC7A11 sensitized HT-1080 cells to erastin-induced death, whereas transfection of HT-1080 cells with a plasmid encoding SLC7A11 conferred protection from erastin- and sulfasalazine-induced death.","RT-qPCR, siRNA, gene transfection, cell viability","Human","HT-1080 cells","SLC7A11 :+: Cystine, Cystine :-: Ferroptosis","Validated","_NA_","Cystine/glutamate transporter","Q9UPY5 (XCT_HUMAN)",22632970,"_NA_"]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Overexpressed in human cancer specimens. Overexpression inhibits ROS-induced ferroptosis.","Western blot, cell death test, ChIP assay, RT-PCR, immunofluorescence staining, colony formation assay, TUNEL and BrdU assay","Human, mice","H1299 cells, mouse embryonic fibroblasts, transgenic mice; colon, liver and kidney tumour samples; mouse xenograft","SLC7A11 :+: Cystine, Cystine :-: Ferroptosis","Validated","_NA_","Cystine/glutamate transporter","Q9UPY5 (XCT_HUMAN)",25799988,"_NA_"]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Overexpression of SLC7A11 considerably abrogated ferroptosis.","Cell death quantification, gene transfection, western blot","Human","H1299 cells","SLC7A11 :-: Ferroptosis","Validated","_NA_","Cystine/glutamate transporter","Q9UPY5 (XCT_HUMAN)",26218928,"_NA_"]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Knockdown increased cell death.","siRNA, western blot, cell death test","Human","MDA MB 231 cells","SLC7A11 :+: GSH, GSH :-: Ferroptosis","Validated","_NA_","Cystine/glutamate transporter","Q9UPY5 (XCT_HUMAN)",27441659,"_NA_"]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Inhibition induces ferroptosis. Silencing of the SLC7A11 gene increases the cisplatin sensitivity of resistant HNC cells.","Cell viability, western blot, siRNA or shRNA, glutathione and ROS production measurements","Human, mice","AMC-HN3R, -HN4R, and -HN9R head and neck cancer cells, BALB/c male nude mice","SLC7A11 :-: Ferroptosis","Validated","_NA_","Cystine/glutamate transporter","Q9UPY5 (XCT_HUMAN)",27477897,"_NA_"]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Elevated levels of expression are resistant to erastin-induced ferroptosis. Repression of SLC7A11 expression by p53 sensitized cells to undergo erastin-induced ferroptosis.","Western blot, cell death assay, qPCR","Human","H1299 cells","SLC7A11 :-: Ferroptosis","Validated","_NA_","Cystine/glutamate transporter","Q9UPY5 (XCT_HUMAN)",27705786,"_NA_"]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Slc7a11 deletion increases susceptibility to iron overload-induced ferroptosis.","Cell viability, RNAi, RT-PCR","Mice","Mice, hepatocytes, bone marrow–derived macrophages","SLC7A11 :-: Ferroptosis","Validated","_NA_","Cystine/glutamate transporter","Q9UPY5 (XCT_HUMAN)",28195347,"_NA_"]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Knockdown sensitized cells to erastin.","shRNA, cell viability","Human","HCT116","SLC7A11 :-: Ferroptosis","Validated","_NA_","Cystine/glutamate transporter","Q9UPY5 (XCT_HUMAN)",28813679,"_NA_"]
["SLC7A11","Solute carrier family 7 member 11","HGNC:11059","Overexperession of SLC7A11 attenuated BAY-inhibited cell viability by ferroptosis.","Cell viability assay, qRT-PCR, shRNA, gene overexpression, western blot","Human","MDA-MB-231,  MDA-MB-468, DBTRG-05MG","SLC7A11 :-: Ferroptosis","Validated","_NA_","Cystine/glutamate transporter","Q9UPY5 (XCT_HUMAN)",29274359,"_NA_"]
["SQSTM1","Sequestosome 1","HGNC:11280","The interaction between p62 and Keap1 increased following erastin and sorafenib treatment. Knockdown of p62 suppressed NRF2 expression and promoted growth inhibition with increased ferroptotic events including GSH depletion, lipid ROS production, and an increase of iron levels.","Immunoprecipitation assay, shRNA knockdown, iron assay, lipid peroxidation assay, glutathione assay, cell viability","Human, mice","HepG2, Hepa1‐6, Hep3B, and SNU‐182 cells","SQSTM1 :-: KEAP1, KEAP1 :-: NFE2L2, NFE2L2 :-: Ferroptosis","Validated","_NA_","Sequestosome-1","Q13501 (SQSTM_HUMAN)",26403645,"p62 in article."]
["SQSTM1","Sequestosome 1","HGNC:11280","Inhibition of the p62 gene significantly reduced cell viability and increased cellular lipid ROS levels in HN3R cells; this was reversed by treatment with ferrostatin-1, a ferroptosis inducer.","siRNA, qPRC, fluorescent microscopy and flow cytometry, ROS production measurement","Human","HN3 cells","SQSTM1 :+: NFE2L2, NFE2L2 :+: FTH1, FTH1 :-: Ferroptosis, NFE2L2 :+: FPN, FPN :-: Ferroptosis, NFE2L2 :+: HO-1, HO-1 :-: Ferroptosis","Validated","Not test cell death or cell viability.","Sequestosome-1","Q13501 (SQSTM_HUMAN)",30339884,"p62 in article."]
["TF","Transferrin","HGNC:11740","Essential for the induction of ferroptotic cell death. Transferrin can only interact with transferrin receptor and be transported into the cell when it is loaded with iron.","Killing activity","Mice","Mouse embryonic fibroblasts","TF :+: Ferroptosis","Validated","_NA_","Serotransferrin","P02787 (TRFE_HUMAN)",26166707,"Transferrin in article"]
["TF","Transferrin","HGNC:11740","Involved in siramesine and lapatinib-induced ferroptotic cell death. Increased following treatment with lapatinib alone or in combination with siramesine. Knocking down of transferrin resulted in decreased cell death and ROS after treatment.","Western blot, densitometry quantification, siRNA, gene transfection, cell death test","Human","MDA MB 231 and SKBr3 cells","TF :+: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","_NA_","Serotransferrin","P02787 (TRFE_HUMAN)",27441659,"Transferrin in article"]
["TFR2","Transferrin receptor 2","HGNC:11762","RNAi of transferrin receptor (TfR) inhibited ferroptosis.","RNAi","Mice","Mouse embryonic fibroblasts","TFR2 :+: Ferroptosis","Validated","_NA_","Transferrin receptor protein 2","Q9UP52 (TFR2_HUMAN)",26166707,"Tfr in article"]
["TP53","Tumor protein p53","HGNC:11998","Inhibits cystine uptake and sensitizes cells to ferroptosis. Erastin induced high levels of cell death in p53+/+ MEFs.","Cell death kinetics, shRNA, western blotting, RT-PCR, ChIP assay, cystine uptake assay","Human, mice","H1299, U2OS and MCF7 cells, mouse embryonic fibroblasts","TP53 :-: SLC7A11, SLC7A11 :+: Cystine, Cystine :-: Ferroptosis","Validated","The acetylation-defective mutant (p53[3KR]) also retains the ability to induce ferroptosis upon reactive oxygen species (ROS)-induced stress.","Cellular tumor antigen p53","P04637 (P53_HUMAN)",25799988,"_NA_"]
["TP53","Tumor protein p53","HGNC:11998","Wild type p53 can induce ferroptosis upon reactive oxygen species (ROS)-induced stress.","Cell death quantification, western blot","Human","H1299 cells","TP53 :-: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","_NA_","Cellular tumor antigen p53","P04637 (P53_HUMAN)",26218928,"p53 in article"]
["TP53","Tumor protein p53","HGNC:11998","p53^3KR/3KR Xrcc4^-/- MEF cells are very sensitive to ferroptosis. Stabilized in the spleens of p53^3KR/3KR Xrcc4^-/- mice which enables to prevent the development of pro-B-cell lymphomas.","Western blot, qRT-PCR, cell viability","Mice","p53^3KR/3KR Xrcc4^-/- MEF cells, p53^3KR/3KR Xrcc4^-/- mice","TP53 :-: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","_NA_","Cellular tumor antigen p53","P04637 (P53_HUMAN)",26943586,"p53 in article"]
["TP53","Tumor protein p53","HGNC:11998","Incubation with erastin led to profound cell death in wild-type MEFs.","Cell viability assay, phase-contrast microscopy","Human, mice","Mouse embryonic fibroblasts, lymphoblastoid cell lines","TP53 :-: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","_NA_","Cellular tumor antigen p53","P04637 (P53_HUMAN)",27034505,"p53 in article"]
["TP53","Tumor protein p53","HGNC:11998","p53 acetylation has a critical role in ferroptotic responses. Simultaneous loss of K98/117/161/162 acetylations is unable to induce ferroptosis, and its ability to thwart cancer growth is also abrogated.","Mass spectrometry screening, western blot, cell death assay","Human, mice","H1299 cells, mouse xenograft models","TP53 :-: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","_NA_","Cellular tumor antigen p53","P04637 (P53_HUMAN)",27705786,"_NA_"]
["TP53","Tumor protein p53","HGNC:11998","Facilitates ART-induced ferroptosis. Conversely, knockdown of P53 blocked ART-induced ferroptosis.","Western blot, immunofluorescence, siRNA","Rat","Hepatic stellate cell HSC‐T6","TP53 :+: Ferroptosis","Validated","_NA_","Cellular tumor antigen p53","P04637 (P53_HUMAN)",30321484,"P53 in article"]
["TP53","Tumor protein p53","HGNC:11998","Inhibits ferroptosis in human colorectal cancer (CRC) cells. Loss of TP53 restored erastin sensitivity. Inhibits cell death induction by erastin in human CRC cells.","RNAi, live-cell imaging, cell viability, qPCR, western blot","Human, mice","HCT116, athymic nude mice","TP53 :+: Nucleus DPP4, Nucleus DPP4 :+: SLC7A11, SLC7A11 :-: Ferroptosis","Validated","Promotes ferroptosis via a transcription-dependent mechanism in non-CRC cells.","Cellular tumor antigen p53","P04637 (P53_HUMAN)",28813679,"_NA_"]
["TP53","Tumor protein p53","HGNC:11998","p53 stabilization suppresses ferroptosis. p53 suppresses metabolic stress-induced ferroptosis.","Immunoblotting, cell death quantification, time-lapse imaging","Human","HT-1080 cells","TP53 :+: CDKN1A, CDKN1A :+: GSH, GSH :-: Lipid ROS, Lipid ROS :+: Ferroptosis","Validated","Found to promote ferroptosis by other studies.","Cellular tumor antigen p53","P04637 (P53_HUMAN)",29346757,"p53 in article"]
["PLIN4","Perilipin 4","HGNC:29393","Upregulated in cells treated with ferroptosis inducer erastin. Stimulates ferroptosis possibly in a GSH-dependent manner.","Tandem mass tags, RNA-seq, DNase-seq, qPCR, Western blot, IHC","Human","HepG2, Bel-7402 cells","Deduced","_NA_","Perilipin-4","Q96Q06 (PLIN4_HUMAN)",31108460,"May promote ferroptosis."]