{"text_format":"3","workbook":"xlsx","n_sheets":7,"sheets":["S1+RBD+ B cell flow panel","37c broad immune flow panel","Imaging","T cell cytokine panel","AIM assay","Sorting S1+ B cells","CITEseq antibodies"],"read":"whole","date_system":"1900"}

{"sheet":"S1+RBD+ B cell flow panel","index":0,"n_rows":28,"n_cols":5,"rows_kept":28}
["Number","Reagent","Source","Catalog number","Clone"]
[1,"Anti-CD11c-BUV395","BD",563787,"B-ly6"]
[2,"Anti-CD71-BUV661","BD",749818,"L01.1"]
[3,"Anti-CD138-BUV737","BD",612834,"MI15"]
[4,"Anti-CD45-BUV805","BD",612891,"HI30"]
[5,"Anti-CD62L-BV750","BD",747422,"DREG-56"]
[6,"Anti-FCRL5/FCRL3-AF647","BD",564343,307307]
[7,"Anti-CD86-AF700","BD",561124,"2331 (FUN-1)"]
[8,"Anti-CD20-APC-H7","BD",560734,"2H7"]
[9,"Anti-CD10-BV510","BD ",563032,"HI10a"]
[10,"Anti-CD19-BV650","BD ",563226,"SJ25C1"]
[11,"Anti-CD95-PE-Cy5","BD ",340480,"DX2"]
[12,"Anti-IgG-PE-Cy7","BD ",561298,"G18-145"]
[13,"Anti-CD3-BV570","BioLegend",300436,"UCHT1"]
[14,"Anti-IgD-BV605","BioLegend",348232,"IA6-2"]
[15,"Anti-IgM-BV711","BioLegend",314540,"MHM-88"]
[16,"Anti-CD27-BV785","BioLegend",302832,"O323"]
[17,"Anti-CD14-Spark Blue 550","BioLegend",367148,"63D3"]
[18,"Anti-CD21-FITC","BioLegend",354910,"Bu32"]
[19,"Live dead dye-Zombie NIR","BioLegend",423106]
[20,"CoV2-RBD-Biotin","BioLegend",793906]
[21,"SAv-BV421","BioLegend",405225]
[22,"CoV2-S1-Biotin","BioLegend",793806]
[23,"Anti-CD38-APC-Fire810","BioLgend",303550,"HIT2"]
[24,"Anti-IgA-VioBlue","Miltenyi","130-113-479","IS11-8E10"]
[25,"SAv-APC","ThermoFisher","S32362"]
[26,"Anti-CD85j-Super bright 436","ThermoFisher","62-5129-42","HP-F1"]
[27,"Anti-FCRL4-PerCP-eFluor710","ThermoFisher","46-3079-42","413D12"]

{"sheet":"37c broad immune flow panel","index":1,"n_rows":39,"n_cols":5,"rows_kept":39}
["Number","Reagent","Source","Catalog Number","Clone"]
[1,"Anti-CD141-BB515","BD",566017,"1A4"]
[2,"Anti-CD16-BUV496","BD",612944,"3G8"]
[3,"Anti-TCR γδ-BB700","BD",745944,"11F2"]
[4,"Anti-CD11c-BUV661","BD",612967,"B-ly6"]
[5,"Anti-IgG-BV605","BD",563246,"G18-145"]
[6,"Anti-CD127-APC-R700","BD",565185,"HIL-7R-M21"]
[7,"Anti-IgD-BV480","BD",566138,"IA6-2"]
[8,"Anti-CD56-BUV737","BD",612766,"NCAM16.2"]
[9,"Anti-CXCR5-BV750","BD",747111,"RF8B2"]
[10,"Anti-CD8-BUV805","BD",612889,"SK1"]
[11,"Anti-ICOS-BUV563","BD",741421,"DX29"]
[12,"Anti-CD45RA-BUV395","BD",740315,"5H9"]
[13,"SAv-BUV615","BD",613013]
[14,"Anti-CD14-Spark Blue 550","BioLegend",367148,"63D3"]
[15,"Anti-CD25-PE","BioLegend",302606,"BC96"]
[16,"Anti-CD28-BV650","BioLegend",302946,"CD28.2"]
[17,"Anti-CD183-PE-Cy7","BioLegend",353720,"G025H7"]
[18,"Anti-PD-1-BV785","BioLegend",329930,"EH12.2H7"]
[19,"Anti-CCR6-BV711","BioLegend",353436,"G034E3 "]
[20,"Anti-CCR7-BV421","BioLegend",353208,"G043H7"]
[21,"Anti-CD19-Spark NIR 685","BioLegend",302270,"HIB19"]
[22,"Anti-CD38-APC/Fire 810","BioLegend",303550,"HIT2"]
[23,"Anti-CD57-FITC","BioLegend",359604,"HNK-1"]
[24,"Anti-CD1c-Alexa Fluor 647","BioLegend",331510,"L161"]
[25,"Anti-HLA-DR-APC-Fire 750","BioLegend",307658,"L243"]
[26,"Anti-IgM-BV570","BioLegend",314517,"MHM-88"]
[27,"Anti-CD24-PE/Dazzle 594","BioLegend",311134,"ML5"]
[28,"Anti-CD3-BV510","BioLegend",317332,"OKT3"]
[29,"Anti-CD21-PerCP CY5.5","BioLegend",354908,"Bu32"]
[30,"Anti-CD4-CF568","Cytek","R7-20041","SK3"]
[31,"Anti-IgA-Biotin","Jackson ImmunoResearch","109-066-011","Polyclonal"]
[32,"Anti-CD123-Super Bright 436","ThermoFisher","62-1239-42","6H6"]
[33,"Anti-CD95-PE-Cy5","ThermoFisher","15-0959-42","DX2"]
[34,"Anti-CD45-PerCP","ThermoFisher","MHCD4531","HI30"]
[35,"Anti-CD20-Pacific Orange","ThermoFisher","MHCD2030","HI47"]
[36,"Anti-CD161-eFluor 450","ThermoFisher","48-1619-42","HP-3G10"]
[37,"Anti-CD27-APC","ThermoFisher","17-0279-42","O323"]
[38,"Live dead dye-Blue","ThermoFisher","L23105"]

{"sheet":"Imaging","index":2,"n_rows":23,"n_cols":8,"rows_kept":23,"merged":[[0,1,0,4]]}
[null,"Reagent",{"$nulls":3},"Source","Catalog number","Clone"]
["Number","Panel","Step","Antibodies","Conjugate"]
[1,"GC",1,"Hoechst 33342","NA","Biotium",40046,"NA"]
[2,"GC",3,"CD20","Alexa Fluor 488","ThermoFisher","53-0202-82","L26"]
[3,"GC",3,"Ki-67 ","Biotin","Novus","NB500-170B","Polyclonal"]
[4,"GC",4,"See Above","Streptavidin Alexa Fluor 532","ThermoFisher","S11224","NA"]
[5,"GC",1,"CD138","Unconjugated","R&D","AF2780","Polyclonal"]
[6,"GC",2,"Donkey anti-goat IgG ","Alexa Fluor 555","ThermoFisher","A21432","Polyclonal"]
[7,"GC",3,"CD68","iFluor 594","Caprico Biotechnologies",1064135,"KP1"]
[8,"GC",1,"CD21 ","Unconjugated","Abcam","Ab240987","SP186"]
[9,"GC",2,"Donkey anti-rabbit IgG","Alexa Fluor 680","ThermoFisher","A10043","Polyclonal"]
[10,"T",1,"Hoechst 33342","NA","Biotium",40046,"NA"]
[11,"T",3,"CD57","Unconjugated","BioLegend",359602,"HNK-1"]
[12,"T",4,"Donkey anti-mouse IgM ","Alexa Fluor 488","Jackson ImmunoResearch","715-545-020","Polyclonal"]
[13,"T",3,"Ki-67 ","Biotin","Novus","NB500-170B","Polyclonal"]
[14,"T",4,"See Above","Streptavidin iFluor 514","AAT Bioquest",16956,"NA"]
[15,"T",1,"CD8","Unconjugated","ThermoFisher","14-0085-82","C8/144B"]
[16,"T",2,"Donkey anti-mouse IgG ","Alexa Fluor 555","ThermoFisher","A31570","Polyclonal"]
[17,"T",1,"CD3","Unconjugated","Abcam","Ab205228","SP7"]
[18,"T",2,"Donkey anti-rabbit IgG","Alexa Fluor 594","ThermoFisher","A21207","Polyclonal"]
[19,"T",3,"HLA-DR ","AF647","Novus","NBP2-47670AF647","LN-3"]
[20,"T",1,"PD-1","Unconjugated","R&D","AF1086","Polyclonal"]
[21,"T",2,"Donkey anti-goat IgG","Alexa Fluor 680","ThermoFisher","A-21084","Polyclonal"]

{"sheet":"T cell cytokine panel","index":3,"n_rows":34,"n_cols":5,"rows_kept":34}
[null,"Reagent","Source","Catalog number","Clone"]
[1,"Anti-CD45RA-BUV395","BD",740315,"5H9"]
[2,"Anti-CD56-BUV737","BD",612766,"NCAM16.2"]
[3,"Anti-CD8-BUV805","BD",612889,"SK1"]
[4,"Anti-CXCR5-BV750","BD",747111,"RF8B2"]
[5,"Anti-CD25-BB515","BD",564467,"2A3"]
[6,"Anti-CD95-BB700","BD",566542,"DX2"]
[7,"Anti-CD103-BUV661","BD",749993,"Ber-ACT8"]
[8,"Anti-CD69-BUV563","BD",748764,"FN50"]
[9,"Anti-CXCR3-PECy5","BD",551128,"1C6/CXCR3"]
[10,"Anti-Granzyme B-PE","BD",561142,"GB11"]
[11,"Anti-IL4-PerCP Cy5.5","BD",561234,"8D4-8"]
[12,"Anti-IL21-Alexa Fluor 647","BD",560493,"3A3-N2.1"]
[13,"SAv-BUV615","BD",613013]
[14,"Anti-CCR7-BV421","BioLgend",353208,"G043H7"]
[15,"Anti-CD3-BV510","BioLgend",344828,"SK7"]
[16,"Anti-CCR6-BV711","BioLgend",353436,"G034E3"]
[17,"Anti-PD1-BV785","BioLgend",329929,"EH12.2H7"]
[18,"Anti-CD57-FITC","BioLgend",359604,"HNK-1"]
[19,"Anti-CD14-Spark Blue 550","BioLgend",367148,"63D3"]
[20,"Anti-HLA-DR-APC/Fire 750","BioLgend",307658,"L243"]
[21,"Anti-CD38-APC/Fire810","BioLgend",303550,"HIT2"]
[22,"Anti-CD19-Spark NIR 685","BioLgend",302270,"HIB19"]
[23,"Anti-IL17A-BV605","BioLgend",512326,"BL168"]
[24,"Anti-IL2-BV650","BioLgend",500334,"MQ1-17H12"]
[25,"Anti-IFNγ-Pacific Blue","BioLgend",502522,"4S.B3 "]
[26,"Anti-IL10-PE/Dazzle 594","BioLgend",506812,"JES3-19F1"]
[27,"Anti-Perforin-APC","BioLgend",353312,"B-D48 "]
[28,"Anti-TNFa-PE-Cy7","BioLgend",502930,"MAb11"]
[29,"Anti-CD107a (LAMP-1) -Biotin","BioLgend",328604,"H4A3 "]
[30,"Anti-CD4-CD568","Cytek","R7-20041","SK3"]
[31,"Anti-CD45-PerCP","ThermoFisher","MHCD4531","HI30"]
[32,"Anti-CD27-Super Bright 436","ThermoFisher","62-0279-42","O323"]
[33,"Live dead dye-Blue","ThermoFisher","L23105"]

{"sheet":"AIM assay","index":4,"n_rows":30,"n_cols":5,"rows_kept":30}
["Number","Reagent","Source","Catalog number","Clone"]
[1,"Anti-CXCR3-PE/Cy5","BD",551128,"1C6/CXCR3"]
[2,"Anti-CXCR5-BV750","BD",747111,"RF8B2"]
[3,"Anti-CD45RA-BUV395","BD",740315,"5H9"]
[4,"Anti-ICOS-BUV563","BD",741421,"DX29"]
[5,"Anti-CD56-BUV737","BD",612766,"NCAM16.2"]
[6,"Anti-CD8-BUV805","BD",612889,"SK1"]
[7,"Anti-CD40L-PE","BD",557299,"TRAP1"]
[8,"Anti-CD25-PE-CF594","BD",562403,"M-A251"]
[9,"Anti-CCR7-BV421","BioLegend",353208,"G043H7"]
[10,"Anti-CCR6-BV711","BioLegend",353436,"G034E3"]
[11,"Anti-CD4-BV605","BioLegend",317438,"OKT4"]
[12,"Anti-CD69-BV650","BioLegend",310934,"FN50"]
[13,"Anti-PD1-BV785","BioLegend",329930,"EH12.2H7"]
[14,"Anti-CD57-FITC","BioLegend",359604,"HNK-1"]
[15,"Anti-CD14-Spark Blue 550","BioLegend",367148,"63D3 "]
[16,"Anti-CD45-PerCP","BioLegend",368506,"2D1"]
[17,"Anti-4-1BB-PeCy7","BioLegend",309818,"4B4-1"]
[18,"Anti-CD19-Spark NIR 685","BioLegend",302270,"HIB19 "]
[19,"Anti-CD38-APC/Fire 810","BioLegend",303550,"HIT2"]
[20,"Anti-HLA-DR-APC/Fire 750","BioLegend",307658,"L243"]
[21,"Anti-OX40-APC","BioLegend",350008,"Ber-ACT35"]
[22,"Live dead dye- Blue","ThermoFisher","L23105"]
[23,"PepTivator SARS-CoV-2 Prot_S+ ","Miltenyi","130-127-311"]
[24,"PepTivator SARS-CoV-2 Prot_S1 ","Miltenyi","130-127-041"]
[25,"PepTivator SARS-CoV-2 Prot_S ","Miltenyi","130-126-700"]
[25,"PepTivator SARS-CoV-2 Prot_N ","Miltenyi","130-126-698"]
[25,"PepTivator SARS-CoV-2 Prot_M ","Miltenyi","130-126-702"]
[26,"CD40 Antibody, anti-human","Miltenyi","130-094-133","130-094-133"]
[27,"PHA-L","Millipore",11249738001]

{"sheet":"Sorting S1+ B cells","index":5,"n_rows":16,"n_cols":5,"rows_kept":16}
["Number","Reagent","Source","Catalog number","Clone"]
[1,"Anti-CD19-PeCy7","BIoLegend",302216,"HIB19 "]
[2,"Anti-CD56-BV510","BIoLegend",362533,"5.1H11 "]
[3,"Anti-CD14-BV510","BIoLegend",367124,"63D3 "]
[4,"Anti-CD3-Alexa Fluor 488","BIoLegend",317310,"OKT3 "]
[5,"Anti-CD8-PE","BIoLegend",303804,"QA18A37"]
[6,"Anti-CD95-PE/Dazzle 594","BIoLegend",305634,"DX2 "]
[7,"CoV2-S1-Biotin","BIoLegend",793806]
[8,"SAv-BV421","BIoLegend",405225]
[9,"Anti-CD45-PerCP-Vio 700","Miltenyi","130-110-636","REA747"]
[10,"Anti-CD4-APC-eFLuor 780","ThermoFisher","47-0048-42","OKT4"]
[11,"Live dead dye-Aqua","ThermoFisher","L34957"]
[12,"SAv-APC","ThermoFisher","S32362"]
[]
[null,"Anti-S1-BV421 was conjugated from Biotin-S1 and SAv-BV421"]
[null,"Anti-S1-APC  was conjugated from Biotin-S1 and SAv-APC"]

{"sheet":"CITEseq antibodies","index":6,"n_rows":24,"n_cols":6,"rows_kept":24}
["Supplementary Table 8: Reagents"]
["Number","Reagent","Source","Catalog number","Barcode","Clone"]
[1,"TotalSeq™-C0384 anti-human IgD Antibody","BioLegend",348245,"CAGTCTCCGTAGAGT","IA6-2 "]
[2,"TotalSeq™-C0389 anti-human CD38 Antibody","BioLegend",303543,"TGTACCCGCTTGTGA","HIT2"]
[3,"TotalSeq™-C0154 anti-human CD27 Antibody","BioLegend",302853,"GCACTCCTGCATGTA","O323"]
[4,"TotalSeq™-C0181 anti-human CD21 Antibody","BioLegend",354923,"AACCTAGTAGTTCGG","Bu32"]
[5,"TotalSeq™-C0053 anti-human CD11c Antibody","BioLegend",371521,"TACGCCTATAACTTG","S-HCL-3"]
[6,"TotalSeq™-C0828 anti-human CD307d (FcRL4) Antibody","BioLegend",340213,"CGATTTGATCTGCCT","413D12"]
[7,"TotalSeq™-C0829 anti-human CD307e (FcRL5) Antibody","BioLegend",340309,"TCACGCAGTCCTCAA","509f6"]
[8,"TotalSeq™-C0826 anti-human CD307c/FcRL3 Antibody","BioLegend",374413,"GCCTAGTTTGAACGC","H5/FcRL3"]
[9,"TotalSeq™-C0006 anti-human CD86 Antibody","BioLegend",305447,"GTCTTTGTCAGTGCA","IT2.2"]
[10,"TotalSeq™-C0147 anti-human CD62L Antibody","BioLegend",304851,"GTCCCTGCAACTTGA","DREG-56 "]
[11,"TotalSeq™-C0146 anti-human CD69 Antibody","BioLegend",310951,"GTCTCTTGGCTTAAA","FN50"]
[12,"TotalSeq™-C0159 anti-human HLA-DR Antibody","BioLegend",307663,"AATAGCGAGCAAGTA","L243"]
[13,"TotalSeq™-C0161 anti-human CD11b Antibody","BioLegend",301359,"GACAAGTGATCTGCA","ICRF44"]
[14,"TotalSeq™-C0359 anti-human CD83 Antibody","BioLegend",305341,"CCACTCATTTCCGGT","HB15e "]
[15,"TotalSeq™-C0088 anti-human CD279 (PD-1) Antibody","BioLegend",329963,"ACAGCGCCGTATTTA","EH12.2H7"]
[16,"TotalSeq™-C0168 anti-human CD57 Recombinant Antibody","BioLegend",393321,"AACTCCCTATGGAGG","\nQA17A04"]
[17,"TotalSeq™-C0145 anti-human CD103 (Integrin αE) Antibody","BioLegend",350233,"GACCTCATTGTGAAT","Ber-ACT8"]
[18,"TotalSeq™-C0180 anti-human CD24 Antibody","BioLegend",311143,"AGATTCCTTCGTGTT","ML5 "]
[19,"TotalSeq™-C0140 anti-human CD183 (CXCR3) Antibody","BioLegend",353747,"GCGATGGTAGATTAT","\nG025H7 "]
[20,"TotalSeq™-C0148 anti-human CD197 (CCR7) Antibody","BioLegend",353251,"AGTTCAGTCAACCGA","G043H7"]
[21,"TotalSeq™-C0143 anti-human CD196 (CCR6) Antibody","BioLegend",353440,"GATCCCTTTGTCACT","G034E3 "]
[22,"TotalSeq™-C0144 anti-human CD185 (CXCR5) Antibody","BioLegend",356939,"AATTCAACCGTCGCC","J252D4"]