ferroptosis gene Name HGNC_ID Evidence Test method Test in Test setting Pathway Confidence Caution Protein encoded UniProtKB PMID Remark ACSL4 Acyl-CoA synthetase long chain family member 4 HGNC:3571 Deletion of this gene likely suppress ferroptosis by limiting the membrane-resident pool of oxidation-sensitive fatty acids. Retroviral-mediated insertional mutagenesis and sequencing Human Chronic myeloid leukemia cell line KBM7 ACSL4 :+: Ferroptosis Predicted Author prediction based on gene function. Long-chain-fatty-acid--CoA ligase 4 O60488 (ACSL4_HUMAN) 25965523 _NA_ AKR1C1 Aldo-keto reductase family 1 member C1 HGNC:384 Up-regulated in DU-145 erastin-resistant clones. Participate in the detoxification of toxic lipid metabolites. May confer partial resistance to erastin by enhancing the detoxification of reactive aldehydes generated downstream of the oxidative destruction of the plasma membrane during ferroptosis. "RNA-seq, glutamate release assay, cell growth, flow cytometry" Human DU-145 prostate cancer cells AKR1C1 :-: Ferroptosis Validated _NA_ Aldo-keto reductase family 1 member C1 Q04828 (AK1C1_HUMAN) 24844246 _NA_ AKR1C2 Aldo-keto reductase family 1 member C2 HGNC:385 Up-regulated in DU-145 erastin-resistant clones. Participate in the detoxification of toxic lipid metabolites. May confer partial resistance to erastin by enhancing the detoxification of reactive aldehydes generated downstream of the oxidative destruction of the plasma membrane during ferroptosis. "RNA-seq, glutamate release assay, cell growth, flow cytometry" Human DU-145 prostate cancer cells AKR1C2 :-: Ferroptosis Validated _NA_ Aldo-keto reductase family 1 member C2 P52895 (AK1C2_HUMAN) 24844246 _NA_ AKR1C3 Aldo-keto reductase family 1 member C3 HGNC:386 Up-regulated in DU-145 erastin-resistant clones. Participate in the detoxification of toxic lipid metabolites. May confer partial resistance to erastin by enhancing the detoxification of reactive aldehydes generated downstream of the oxidative destruction of the plasma membrane during ferroptosis. "RNA-seq, glutamate release assay, cell growth, flow cytometry" Human DU-145 prostate cancer cells AKR1C3 :-: Ferroptosis Validated _NA_ Aldo-keto reductase family 1 member C3 P42330 (AK1C3_HUMAN) 24844246 _NA_ ALOX15 Arachidonate 15-lipoxygenase HGNC:433 Silencing ALOX genes made cells resistant to ferroptosis. qPCR Human G-401 cells "ALOX15 :+: PUFAs peroxidation, PUFAs peroxidation :+: Ferroptosis" Validated _NA_ Arachidonate 15-lipoxygenase P16050 (LOX15_HUMAN) 27506793 _NA_ ALOX5 Arachidonate 5-lipoxygenase HGNC:435 The 5-Lipoxygenase inhibitor zileuton protected HT22 neuronal cells from erastin-induced ferroptosis. "Cell Viability Assay, ROS measurement" Mice HT22 mouse neuronal cell line "ALOX5 :+: Lipid ROS, Lipid ROS :+: Ferroptosis" Validated _NA_ Arachidonate 5-lipoxygenase P09917 (LOX5_HUMAN) 26235588 5-LOX in article ALOX12 "Arachidonate 12-lipoxygenase, 12S type" HGNC:429 Silencing ALOX genes made cells resistant to ferroptosis. qPCR Human G-401 cells "ALOX12 :+: PUFAs peroxidation, PUFAs peroxidation :+: Ferroptosis" Validated _NA_ Arachidonate 12-lipoxygenase P18054 (LOX12_HUMAN) 27506793 _NA_ ATP5MC3 ATP synthase membrane subunit c locus 3 HGNC:843 Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis. "shRNA screening, qPCR, gene silencing" Human NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell ATP5MC3 :+: Ferroptosis Validated _NA_ ATP synthase F(0) complex subunit C3 P48201 (AT5G3_HUMAN) 22632970 ATP5G3 in article CARS1 Cysteinyl-tRNA synthetase 1 HGNC:1493 "Required for ferroptosis in diverse cell contexts. Knockdown of CARS inhibited erastin-induced death by preventing the induction of lipid reactive oxygen species, without altering iron homeostasis." "siRNA screen, shRNA, western blot, cell viability" "Human, rat" "HT-1080 fibrosarcoma cells,BJeLR fibroblast cells, Panc1 cells, A673 (Ewing¡¯s sarcoma) and 143B (osteosarcoma) cells, PC12 cells" "CARS1 :-: Transsulfuration pathway, Transsulfuration pathway :-: Ferroptosis" Validated _NA_ Cysteine--tRNA ligase P49589 (SYCC_HUMAN) 26184909 CARS in article CBS Cystathionine beta-synthase HGNC:1550 Inhibition triggers ferroptosis in hepatocellular carcinoma. "Cell viability, western blotting, LC/ESI-MS/MS, flow cytometry, xenograft mice" "Human, mice" "HepG2 cells, female ICR mice" CBS :-: Ferroptosis Validated _NA_ Cystathionine beta-synthase P35520 (CBS_HUMAN) 30258181 _NA_ CD44 CD44 molecule (Indian blood group) HGNC:1681 Knockdown sensitizes cells to ferroptosis. "Western blot, siRNA, cell death" Human H1299 cells "CD44 :+: SLC7A11, SLC7A11 :-: Ferroptosis" Validated _NA_ CD44 antigen P16070 (CD44_HUMAN) 30709928 _NA_ CHAC1 ChaC glutathione specific gamma-glutamylcyclotransferase 1 HGNC:28680 CHAC1 degradation of GSH might enhance cystine-starvation-induced cell death. "Western blot, real-time PCR, cell viability assay, siRNA, GSH assay kit" Human "MDA-MB-231, Hs 578T, and HCC 1937 cells" "CHAC1 :-: GSH, GSH :-: Ferroptosis" Validated _NA_ Glutathione-specific gamma-glutamylcyclotransferase 1 Q9BUX1 (CHAC1_HUMAN) 29383104 _NA_ CISD1 CDGSH iron sulfur domain 1 HGNC:30880 Genetic inhibition of CISD1 contributes to erastin-induced ferroptosis. Stabilization of the iron sulfur cluster of CISD1 inhibits ferroptosis. "Cytotoxicity assays, western blot, q-PCR, RNAi" Human HepG2 and Hep3B cells "CISD1 :-: Mitochondrial lipid ROS, Mitochondrial lipid ROS :+: Ferroptosis" Validated _NA_ CDGSH iron-sulfur domain-containing protein 1 Q9NZ45 (CISD1_HUMAN) 27510639 _NA_ CS Citrate synthase HGNC:2422 Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis. "shRNA screening, qPCR, gene silencing" Human NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell CS :+: Ferroptosis Validated _NA_ Citrate synthase O75390 (CISY_HUMAN) 22632970 _NA_ DPP4 Dipeptidyl peptidase 4 HGNC:3009 Required for ferroptosis in TP53-deficient CRC cells. "Cell death, RNAi, western blot, enzyme activity" "Human, mice" "TP53-/- HCT116 cells, athymic nude mice" "DPP4 :+: Lipid ROS, Lipid ROS :+: Ferroptosis" Validated _NA_ Dipeptidyl peptidase 4 P27487 (DPP4_HUMAN) 28813679 _NA_ FANCD2 FA complementation group D2 HGNC:3585 Inhibits erastin-induced ferroptosis. Plays a novel role in the negative regulation of ferroptosis. "Cell viability assay, Q-PCR, western blot, iron assay, lipid peroxidation assay, glutathione assay" Mice Bone marrow stromal cells FANCD2 :-: Ferroptosis Validated _NA_ Fanconi anemia group D2 protein Q9BXW9 (FACD2_HUMAN) 27773819 _NA_ GCLC Glutamate-cysteine ligase catalytic subunit HGNC:4311 RNAi knockdown sensitized cell death induced by cystine starvation. "RNAi, qPCR" Mice Mouse embryonic fibroblasts "GCLC :+: GSH, GSH :-: Ferroptosis" Validated _NA_ Glutamate--cysteine ligase catalytic subunit P48506 (GSH1_HUMAN) 26166707 _NA_ GCLM glutamate-cysteine ligase modifier subunit "Chromosome 1, NC_000001.11 (93885199..93910370, complement)" Validated _NA_ GLS2 Glutaminase 2 HGNC:29570 The gene targets of the enriched shRNAs are potential genes that positively regulate ferroptosis. RNAi screening Mice Mouse embryonic fibroblasts "GLS2 :+: Lipid ROS, Lipid ROS :+: Ferroptosis" Validated _NA_ Glutaminase liver isoform Q9UI32 (GLSL_HUMAN) 27514700 _NA_ GPX4 Glutathione peroxidase 4 HGNC:4556 RNAi-mediated GPX4 knockdown induces ferroptosis. "Affinity-based chemoproteomics, western blotting, and RNAi" "Human, mice" "HRAS mutant BJeLR-engineered tumor cells, HT-1080 cells, xenograft mouse model (athymic nude mice injected with four million HT-1080 cells)" "GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis" Validated _NA_ Phospholipid hydroperoxide glutathione peroxidase P36969 (GPX4_HUMAN) 24439385 _NA_ GSS glutathione synthetase Human Validated _NA_ HMGCR 3-hydroxy-3-methylglutaryl-CoA reductase Human Validated _NA_ HSPB1 Heat shock protein family B (small) member 1 HGNC:5246 "Knockdown of HSF1 and HSPB1 enhances erastin-induced ferroptosis, whereas heat shock pretreatment and overexpression of HSPB1 inhibits erastin-induced ferroptosis." "RNAi, RT-qPCR, western blot, cell viability" "Human, mice" "HeLa and U2OS cells, human xenograft mouse tumor derived from HeLa (#CCL-2) cells." HSPB1 :-: Ferroptosis Validated _NA_ Heat shock protein beta-1 P04792 (HSPB1_HUMAN) 25728673 _NA_ CRYAB crystallin alpha B Human Validated _NA_ LPCAT3 Lysophosphatidylcholine acyltransferase 3 HGNC:30244 Knockdown of Lpcat3 increased resistance to ferroptosis triggered by RSL3. shRNA Mice Mouse lung epithelial (MLE) and embryonic cells LPCAT3 :+: Ferroptosis Validated _NA_ Lysophospholipid acyltransferase 5 Q6P1A2 (MBOA5_HUMAN) 27842066 _NA_ MT1G Metallothionein 1G HGNC:7399 "A negative regulator of ferroptosis in HCC cells. Knockdown of MT©\1G by RNA interference increases glutathione depletion and lipid peroxidation, which contributes to sorafenib©\induced ferroptosis." "RNA interference, cell viability assay, colony formation assay, western blot, qRT-PCR, glutathione assay, lipid peroxidation assay" "Human, mice" "HCC cells (HepaG2, Hep3B, and Huh7 cells), nude mice." MT1G :-: Ferroptosis Validated _NA_ Metallothionein-1G P13640 (MT1G_HUMAN) 27015352 MT©\1G in article NCOA4 Nuclear receptor coactivator 4 HGNC:7671 "Inhibition suppressed ferroptosis, and overexpression promoted ferroptosis." "shRNA, gene transfection" Human PANC1 or HT-1080 cells "NCOA4 :-: FTH1, FTH1 :-: Ferroptosis" Validated _NA_ Nuclear receptor coactivator 4 Q13772 (NCOA4_HUMAN) 27245739 _NA_ PTGS2 Prostaglandin-endoperoxide synthase 2 HGNC:9605 "Simply a downstream marker of ferroptosis. The most upregulated gene in BJeLR cells upon treatment with either erastin or (1S, 3R)-RSL3, but ferroptotic cell death was not affected by inhibition of the enzyme." "RT-qPCR, cell viability" Human BJeLR cells Validated _NA_ Prostaglandin G/H synthase 2 P35354 (PGH2_HUMAN) 24439385 May promote ferroptosis. RPL8 Ribosomal protein L8 HGNC:10368 Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis. "shRNA screening, qPCR, gene silencing" Human NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell RPL8 :+: Ferroptosis Validated _NA_ 60S ribosomal protein L8 P62917 (RL8_HUMAN) 22632970 _NA_ SAT1 Spermidine/spermine N1-acetyltransferase 1 HGNC:10540 p53-mediated activation of SAT1 contributes to ferroptotic cell death in the presence of ROS stress. Knockdown of Sat1 partially rescued ROS-induced ferroptosis. "qRT-PCR, siRNA, CRISPR-cas9, western blot" "Human, mice" "H1299, U2OS cells, mouse embryonic fibroblasts, mouse xenograft" "SAT1 :+: ALOX15, ALOX15 :+: Lipid ROS, Lipid ROS :+: Ferroptosis" Validated Activated by p53. Diamine acetyltransferase 1 P21673 (SAT1_HUMAN) 27698118 _NA_ TP53 Tumor protein p53 HGNC:11998 Inhibits cystine uptake and sensitizes cells to ferroptosis. Erastin induced high levels of cell death in p53+/+ MEFs. "Cell death kinetics, shRNA, western blotting, RT-PCR, ChIP assay, cystine uptake assay" "Human, mice" "H1299, U2OS and MCF7 cells, mouse embryonic fibroblasts" "TP53 :-: SLC7A11, SLC7A11 :+: Cystine, Cystine :-: Ferroptosis" Validated The acetylation-defective mutant (p53[3KR]) also retains the ability to induce ferroptosis upon reactive oxygen species (ROS)-induced stress. Cellular tumor antigen p53 P04637 (P53_HUMAN) 25799988 _NA_ FDFT1 farnesyl-diphosphate farnesyltransferase 1 "Chromosome 14, NC_000080.7 (63382599..63419136," Validated TFRC Transferrin receptor HGNC:11763 The gene targets of the enriched shRNAs are potential genes that positively regulate ferroptosis. RNAi screening Mice Mouse embryonic fibroblasts "TFRC :+: Lipid ROS, Lipid ROS :+: Ferroptosis" Screened _NA_ Transferrin receptor protein 1 P02786 (TFR1_HUMAN) 27514700 _NA_ EMC2 ER membrane protein complex subunit 2 HGNC:28963 Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis. "shRNA screening, qPCR, gene silencing" Human NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell EMC2 :+: Ferroptosis Validated _NA_ ER membrane protein complex subunit 2 Q15006 (EMC2_HUMAN) 22632970 TTC35 in article AIFM2 Apoptosis inducing factor mitochondria associated 2 HGNC:21411 A glutathione-independent ferroptosis suppressor. Pharmacological targeting of FSP1 strongly synergizes with GPX4 inhibitors to trigger ferroptosis. "Expression cloning, cell death, cell viability, LDH assay, western blot, LC-MS, NADH consumption assay" "Human, mice" "MCF-7, HT1080, Pfa1 cells" "FPS1 :+: CoQ10, CoQ10 :-: Lipid ROS, Lipid ROS :+: Ferroptosis" Validated _NA_ Apoptosis-inducing factor 2 Q9BRQ8 (AIFM2_HUMAN) 31634899 FSP1 in article PHKG2 Phosphorylase kinase catalytic subunit gamma 2 HGNC:8931 U-2-OS cells became resistant to erastin upon PHKG2 silencing. Erastin-treated HT-1080 cells were rescued by shPHKG2. "shRNA suppressor screen, qPCR, cellular iron staining, cell viability" Human "U-2-OS, HT-1080 cells" "PHKG2 :+: PUFAs peroxidation, PUFAs peroxidation :+: Ferroptosis" Validated _NA_ Phosphorylase b kinase gamma catalytic chain P15735 (PHKG2_HUMAN) 27506793 _NA_ HSBP1 heat shock factor binding protein 1 Validated _NA_ ACO1 Aconitase 1 HGNC:117 The gene targets of the enriched shRNAs are potential genes that positively regulate ferroptosis. RNAi screening Mice Mouse embryonic fibroblasts "ACO1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis" Screened _NA_ Cytoplasmic aconitate hydratase P21399 (ACOC_HUMAN) 27514700 _NA_ FTH1 Ferritin heavy chain 1 HGNC:3976 "Knockdown of p62, quinone oxidoreductase©\1, heme oxygenase©\1, and ferritin heavy chain©\1 by RNA interference in HCC cells promoted ferroptosis in response to erastin and sorafenib." "qRT©\PCR, RNA interference" "Human, mice" "HepG2, Hepa1©\6, Hep3B, and SNU©\182 cells" FTH1 :-: Ferroptosis Validated _NA_ Ferritin heavy chain P02794 (FRIH_HUMAN) 26403645 _NA_ STEAP3 STEAP3 metalloreductase HGNC:24592 Erastin-induced mRNA expression is downregulated in FANCD2-deficient cells. Q-PCR Mice Bone marrow stromal cells Deduced _NA_ Metalloreductase STEAP3 Q658P3 (STEA3_HUMAN) 27773819 May inhibit ferroptosis. NFS1 NFS1 cysteine desulfurase HGNC:15910 Suppression of NFS1 cooperates with inhibition of cysteine transport to trigger ferroptosis in vitro and slow tumour growth. Suppression of NFS1 predisposes cancer cells to ferroptosis. "RNAi, cell viability assay,flow cytometry" Human "MDA-MB-231 cells, NOD.CB17 Scid/J mice" "NFS1 :-: TFRC, TFRC :+: Ferroptosis" Validated _NA_ Cysteine desulfurase Q9Y697 (NFS1_HUMAN) 29168506 _NA_ ACSL3 Acyl-CoA synthetase long chain family member 3 HGNC:3570 Required for exogenous monounsaturated fatty acids to protect cells against ferroptosis. Negatively correlates with ferroptosis sensitivity. "Dead cell count, gene knockout, confocal imaging, thin-layer chromatography, BODIPY 493/503 imaging, CRISPR/Cas9, RT-qPCR, western blot" Human "HEK293, HT-1080 cells" ACSL3 :-: Ferroptosis Validated _NA_ Long-chain-fatty-acid--CoA ligase 3 O95573 (ACSL3_HUMAN) 30686757 _NA_ ACACA acetyl-CoA carboxylase alpha Validated _NA_ PEBP1 Phosphatidylethanolamine binding protein 1 HGNC:8630 Elevated levels of PEBP1 resulted in increased sensitivity of HK2 cells to RSL3 whereas lowered contents of PEBP1 in HAEC and HT22 cells were associated with decreased sensitivity to ferroptosis. "Western blot, siRNA, cell death assay" "Human, mice" "HK2, HAEC and HT22 cells" (PEBP1/15LO) :+: Ferroptosis Validated _NA_ Phosphatidylethanolamine-binding protein 1 P30086 (PEBP1_HUMAN) 29053969 _NA_ ZEB1 Zinc finger E-box binding homeobox 1 HGNC:11642 Knockout of ZEB1 prevents cell death induced by GPX4 inhibition. "sgRNA, cell viability" Human KP4 pancreatic cancer cells "ZEB1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis" Validated _NA_ Zinc finger E-box-binding homeobox 1 P37275 (ZEB1_HUMAN) 28678785 _NA_ SQLE squalene epoxidase Validated _NA_ FADS2 Fatty acid desaturase 2 HGNC:3575 Depletion of the SCD1 and FADS2 metabolic genes induces ferroptosis. "shRNA, RT-qPCR, MTT assay, total iron detection, lipid ROS detection" Human A549 cancer cells FADS2 :-: Ferroptosis Validated _NA_ Acyl-CoA 6-desaturase O95864 (FADS2_HUMAN) 28900510 _NA_ NFE2L2 "Nuclear factor, erythroid 2 like 2" HGNC:7782 Required for sorafenib©\induced expression of MT©\1G which is a ferroptosis suppressor. "shRNA, qRT-PCR, western blot" Human Huh7 and/or HepG2 cells "NFE2L2 :+: MT1G, MT1G :-: Ferroptosis" Deduced "Its ability to induce MT1G was validated, but its role in ferroptosis needs validation." Nuclear factor erythroid 2-related factor 2 Q16236 (NF2L2_HUMAN) 27015352 NRF2 in article KEAP1 Kelch like ECH associated protein 1 HGNC:23177 Knockdown of Keap1 reversed loss of p62-increased degradation of NRF2 in ferroptosis. Keap1 knockdown led to resistance to erastin-induced and sorafenib-induced growth inhibition with decreased ferroptotic events in the absence or presence of p62 knockdown. "Western blot, shRNA knockdown, cell viability" "Human, mice" "HepG2, Hepa1©\6, Hep3B, and SNU©\182 cells" "KEAP1 :-: NFE2L2, NFE2L2 :-: Ferroptosis" Validated _NA_ Kelch-like ECH-associated protein 1 Q14145 (KEAP1_HUMAN) 26403645 _NA_ NQO1 NAD(P)H quinone dehydrogenase 1 HGNC:2874 "Knockdown of p62, quinone oxidoreductase©\1, heme oxygenase©\1, and ferritin heavy chain©\1 by RNA interference in HCC cells promoted ferroptosis in response to erastin and sorafenib." "qRT©\PCR, RNA interference" "Human, mice" "HepG2, Hepa1©\6, Hep3B, and SNU©\182 cells" NQO1 :-: Ferroptosis Validated _NA_ NAD(P)H dehydrogenase [quinone] 1 P15559 (NQO1_HUMAN) 26403645 _NA_ NOX1 Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) 1 HGNC:7889 Suppresses erastin-induced ferroptosis when inhibited by inhibitor. Inhibition test by diphenylene iodonium (DPI) and GKT137831. Human KRAS mutant Calu-1 non small cell lung cancer cell NOX1 :+: Ferroptosis Deduced The presented inhibition study is not sufficient to confirm its role in ferroptosis. NADPH oxidase 1 Q9Y5S8 (NOX1_HUMAN) 22632970 _NA_ NOX1 Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) 1 HGNC:7889 Suppresses erastin-induced ferroptosis when inhibited by inhibitor. Inhibition test by diphenylene iodonium (DPI) and GKT137831. Human KRAS mutant Calu-1 non small cell lung cancer cell NOX1 :+: Ferroptosis Deduced The presented inhibition study is not sufficient to confirm its role in ferroptosis. NADPH oxidase 1 Q9Y5S8 (NOX1_HUMAN) 22632970 _NA_ SLC1A5 Solute carrier family 1 member 5 HGNC:10943 Pharmacological inhibition by L-g-glutamyl-p-nitroanilide or RNAi knockdown markedly blocked ferroptosis. "RNAi knockdown, cell survival analysis" Mice Mouse embryonic fibroblasts "SLC1A5 :+: Glutamine, Glutamine :+: Glutamate, Glutamate :+: alpha-Ketoglutarate, alpha-Ketoglutarate :+: Ferroptosis" Validated _NA_ Neutral amino acid transporter B(0) Q15758 (AAAT_HUMAN) 26166707 _NA_ GOT1 Glutamic-oxaloacetic transaminase 1 HGNC:4432 RNAi reduced ferroptosis. "shRNA, qPCR, cell viability" Mice Mouse embryonic fibroblasts "GOT1 :+: alpha-Ketoglutarate, alpha-Ketoglutarate :+: Ferroptosis" Validated _NA_ Aspartate aminotransferase P17174 (AATC_HUMAN) 26166707 _NA_ G6PD Glucose-6-phosphate dehydrogenase HGNC:4057 Required in erastin-induced ferroptosis. "shRNA silencing, RT-qPCR" Human KRAS mutant Calu-1 non small cell lung cancer cell G6PD :+: Ferroptosis Validated _NA_ Glucose-6-phosphate 1-dehydrogenase P11413 (G6PD_HUMAN) 22632970 _NA_ PGD Phosphoglycerate dehydrogenase HGNC:8891 Required in erastin-induced ferroptosis. "shRNA silencing, RT-qPCR" Human KRAS mutant Calu-1 non small cell lung cancer cell PGD :+: Ferroptosis Validated _NA_ 6-phosphogluconate dehydrogenase P52209 (6PGD_HUMAN) 22632970 _NA_ IREB2 Iron response element binding protein 2 HGNC:6115 Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis. "shRNA screening, qPCR, gene silencing" Human NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell IREB2 :+: Ferroptosis Validated _NA_ Iron-responsive element-binding protein 2 P48200 (IREB2_HUMAN) 22632970 _NA_ HMOX1 Heme oxygenase 1 HGNC:5013 "Zinc protoporphyrin IX, a HO-1 inhibitor, prevented Erastin-triggered ferroptotic cancer cell death. Overexpression accelerates erastin-induced cell death." "Cell viability assay, qRT-PCR, western blot" "Human, mice" "HT-1080 fibrosarcoma cells, mice lung fibroblastic cells" "HMOX1 :+: Lipid ROS, Lipid ROS :+: Ferroptosis" Validated "Other articles (PMID 26403645, 28515173) suggest it a suppressor." Heme oxygenase 1 P09601 (HMOX1_HUMAN) 26405158 HO-1 in article ACSF2 Acyl-CoA synthetase family member 2 HGNC:26101 Required in erastin-induced ferroptosis. Silencing of it conferred against erastin-induced ferroptosis. "shRNA screening, qPCR, gene silencing" Human NRAS mutant HT-1080 fibrosarcoma cells; KRAS mutant Calu-1 non small cell lung cancer cell ACSF2 :+: Ferroptosis Validated _NA_ Medium-chain acyl-CoA ligase ACSF2 Q96CM8 (ACSF2_HUMAN) 22632970 _NA_ GPX4 Glutathione peroxidase 4 HGNC:4556 RNAi-mediated GPX4 knockdown induces ferroptosis. "Affinity-based chemoproteomics, western blotting, and RNAi" "Human, mice" "HRAS mutant BJeLR-engineered tumor cells, HT-1080 cells, xenograft mouse model (athymic nude mice injected with four million HT-1080 cells)" "GPX4 :-: Lipid ROS, Lipid ROS :+: Ferroptosis" Validated _NA_ Phospholipid hydroperoxide glutathione peroxidase P36969 (GPX4_HUMAN) 24439385 _NA_