LOCUS       OCS_4-1                14627 bp    DNA     circular UNA 04-JUN-2021
DEFINITION  Golden Gate/Type IIS Cloning ligation product.
ACCESSION   urn.local...1u1-dfpud89
VERSION     urn.local...1u1-dfpud89
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    6..1010
                     /Source="referenceToDocument: urn:local:.:1tf-dfpuc5t"
                     /label="TU1-1 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    6..30
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK002"
                     /Transferred_Similarity="100.00%"
                     /label="ConS scar"
     misc_feature    36..540
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="U6 promoter paper"
     misc_feature    541..560
                     /created_by="User"
                     /label="gRNA-1"
     misc_feature    561..640
                     /ApEinfo_revcolor="#4764ff"
                     /ApEinfo_fwdcolor="#4764ff"
                     /label="gRNA Handle"
     misc_feature    641..1002
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /label="U6 terminator paper"
     misc_feature    1007..3156
                     /Source="referenceToDocument: urn:local:.:1sz-dfpuauq"
                     /label="TU2-12 (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1007..1031
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK003"
                     /Transferred_Similarity="100.00%"
                     /label="Con1 scar"
     promoter        1037..1170
                     /created_by="User"
                     /modified_by="User"
                     /label="ATF1-2 promoter"
     Misc._binding   1038..1057
                     /created_by="User"
                     /label="gRNA1-target"
     Misc._binding   1067..1086
                     /created_by="User"
                     /label="gRNA1-target"
     Misc._binding   1096..1115
                     /created_by="User"
                     /label="gRNA1-target"
     promoter        <1125..1170
                     /modified_by="User"
                     /label="minimal 35S"
     Misc._binding   1171..1177
                     /label="CaMV 5' UTL"
     Misc._binding   1171
                     /label="Start of transcription"
     5'UTR           1178..1270
                     /modified_by="User"
                     /label="CMV1 5'UTR"
     CDS             1277..2929
                     /created_by="User"
                     /modified_by="User"
                     /label="Luciferase"
     terminator      2945..3148
                     /label="35S ter"
     misc_feature    3153..8299
                     /Source="referenceToDocument: urn:local:.:1tg-dfpuc5u"
                     /label="TU3E-1 (concatenated sequence 4)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    3157..3177
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK004"
                     /Transferred_Similarity="100.00%"
                     /label="Con2 scar"
     promoter        3183..3601
                     /modified_by="User"
                     /label="CaMV 35 S promoter (short)"
     Misc._binding   3602..3608
                     /label="CaMV 5' UTL"
     Misc._binding   3602
                     /label="Start of transcription"
     5'UTR           3609..3701
                     /modified_by="User"
                     /label="CMV1 5'UTR"
     source          <3708..>8069
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             3711..3731
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             3732..7832
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II
                     CRISPR/Cas system"
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC
                     catalytic domain and the H840A mutation in the HNH
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
                     SFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHM
                     IKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKS
                     RRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDN
                     LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLL
                     KALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKL
                     NREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY
                     VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP
                     KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKED
                     YFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTL
                     FEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFL
                     KSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTV
                     KVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEH
                     PVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVL
                     TRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAG
                     FIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFY
                     KVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSM
                     PQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVA
                     KVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFE
                     LENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHK
                     HYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAP
                     AAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /label="dCas9"
     CDS             7851..7871
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             7875..7895
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             7899..7919
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             7920..8069
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDML"
                     /label="VP64"
     terminator      8088..8291
                     /label="35S ter"
     misc_feature    8296..9
                     /Source="referenceToDocument: urn:local:.:1tc-dfpuc5t"
                     /label="pAPT.Ex.KanR Copy (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    8296..8320
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK073, pYTK096"
                     /Transferred_Similarity="100.00%"
                     /label="ConE scar"
     misc_feature    complement(8338..8465)
                     /label="RB short"
     rep_origin      complement(8484..9098)
                     /modified_by="User"
                     /label="rep"
     rep_origin      8499
                     /label="ORI"
     CDS             complement(9261..10052)
                     /label="Kan (no BpiI)"
     mutation        9570
                     /label="A to G (BpiI removed)"
     rep_origin      complement(10238..13022)
                     /modified_by="User"
                     /label="pVS1ori short"
     misc_feature    13024..13171
                     /label="LB"
     misc_feature    13220..13519
                     /label="nos prom"
                     /note="Geneious type: promoter uukaryotic"
     CDS             13532..14323
                     /modified_by="User"
                     /label="KanR"
     terminator      14341..14608
                     /label="nos term"
ORIGIN      
        1 ctagactgaa ctggccgata attgcagacg aacgttcgac gtaaagcctg tagaagaggt
       61 ttctagcgaa cgacacgagt ttgagcctca tgaagcttcg ttgaacaacg gaaactcgac
      121 ttgccttccg cacaatacat catttcttct tagctttttt tcttcttctt cgttcataca
      181 gttttttttt gtttatcagc ttacattttc ttgaaccgta gctttcgttt tcttcttttt
      241 aactttccat tcggagtttt tgtatcttgt ttcatagttt gtcccaggat tagaatgatt
      301 aggcatcgaa ccttcaagaa tttgattgaa taaaacatct tcattcttaa gatatgaaga
      361 taatcttcaa aaggcccctg ggaatctgaa agaagagaag caggcccatt tatatgggaa
      421 agaacaatag tatttcttat ataggcccat ttaagttgaa aacaatcttc aaaagtccca
      481 catcgcttag ataagaaaac gaagctgagt ttatatacag ctagagtcga agtagtgatt
      541 aaaccacaat ttgcacaccc gttttagagc tagaaatagc aagttaaaat aaggctagtc
      601 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttgcaaaat tttccagatc
      661 gatttcttct tcctctgttc ttcggcgttc aatttctggg tttttctctt cgttttctgt
      721 aactgaaacc taaaatttga cctaaaaaaa atctcaaata atatgattca gtggttttgt
      781 acttttcagt tagttgagtt ttgcagttcc gatgagataa accaataact ttgcttagat
      841 ctaattcatt ccgttacacc tctgatggag atggaaggtt cttaataatg atgccatttt
      901 ttgggtaata attttgaatt agaatcaagg gtataagatt cataattaac atcacttaag
      961 caaagttcgt aatatacgac cacaggatat aatttttggt acgctgccaa accagatgtc
     1021 aacacagcta caacgccaaa ccacaatttg cacaccctgg catctcaaac cacaatttgc
     1081 acaccctggc atctcaaacc acaatttgca caccctggca tctccgcaag acccttcctc
     1141 tatataagga agttcatttc atttggagag gacacgcgtt tatttacaag agcgtacggt
     1201 tcaatccctg cctcccctgt aaaactaccc tttgaaaacc tctctttctt aatcttttct
     1261 ttgtaattcc agatctatgg aagatgcaaa gaatatcaaa aaaggcccag cgcccttcta
     1321 cccattagaa gatggaaccg caggagagca acttcacaag gcgatgaaac gatatgctct
     1381 tgtcccggga accatcgctt ttacggacgc acacatagag gttaacatta cctatgcgga
     1441 atattttgaa atgtcagtca gattagcaga agcaatgaaa cgttatgggc tcaacactaa
     1501 ccatcgtatt gttgtatgta gcgaaaacag cctgcagttt tttatgccgg tgctcggtgc
     1561 gctgttcatc ggtgtagcgg ttgctcccgc aaacgacatt tacaacgaga gagaactgct
     1621 taacagcatg aatatcagcc aaccgaccgt cgtgtttgtc tcaaaaaagg gactacaaaa
     1681 aattctaaat gtccaaaaga agttacctat tatccagaaa attattatta tggatagcaa
     1741 gacggattat caaggattcc aatctatgta cacatttgtt acgagccact tacctccagg
     1801 ttttaacgaa tatgattttg tgcctgagag ctttgatcga gataagacca tcgcgttaat
     1861 tatgaatagt tccggctcta cggggctccc aaagggagtc gcactaccac atcgaactgc
     1921 gtgcgttaga ttttcacatg ccagagatcc tatcttcggg aatcagatta ttccggacac
     1981 tgcaatactg agtgtggttc cgtttcatca cgggttcggg atgttcacga cactcggtta
     2041 cctcatatgc ggatttcgtg tggtgctgat gtataggttt gaagaagagt tgttcctaag
     2101 atccttgcag gattacaaaa ttcagtccgc cctgttagtt cctaccttat tttccttttt
     2161 cgccaagtca acgttaattg ataaatatga cctatccaac ctccacgaaa ttgccagtgg
     2221 tggggccccc ttgtccaaag aagttggtga ggcagtcgct aagaggttcc acctgccggg
     2281 tatccgtcaa ggctacggac ttaccgaaac aacctccgct attcttatta cacctgaagg
     2341 cgatgataag ccgggtgctg tcggtaaggt ggtgcctttt tttgaggcga aggtagtgga
     2401 cttagatact ggcaagacgc tcggagttaa tcaacgaggc gagctctgtg tccgtggtcc
     2461 catgataatg agcggatacg tcaacaatcc tgaggcaacc aacgcattaa ttgataaaga
     2521 cggctggttg catagcgggg atatcgcgta ttgggatgag gacgaacact tctttatagt
     2581 cgataggtta aagtcactta ttaagtataa aggttatcaa gtcgctcccg ccgaactgga
     2641 gagtattctt cttcagcatc cgaatatctt tgacgccggt gttgcaggtc ttcctgacga
     2701 cgacgccgga gaactacctg cagcggtcgt ggtgctcgaa catggaaaga caatgaccga
     2761 gaaggagatt gtagattacg tagcttcaca ggtcaccacc gctaaaaaac taagaggtgg
     2821 tgttgtcttc gtagatgaag tgcctaaagg acttaccggt aaactcgacg ccaggaagat
     2881 aagagaaatc ctgatcaaag cgaagaaggg tggtaagtct aagctgtaag gatcctaact
     2941 cgagctctag ctagagtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt
     3001 tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa
     3061 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     3121 aaccaaaatc cagtactaaa atccagatgc tggatgcaca cactggctta agatgacaac
     3181 gcgtcaacat ggtggagcac gacactctgg tctactccaa aaatgtcaaa gatacagtct
     3241 cagaagatca aagggctatt gagacttttc aacaaaggat aatttcggga aacctcctcg
     3301 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct
     3361 cctacaaatg ccatcattgc gataaaggaa aggctatcat tcaagatctc tctgccgaca
     3421 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gaggttccaa
     3481 ccacgtctac aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac
     3541 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     3601 ggacacgcgt ttatttacaa gagcgtacgg ttcaatccct gcctcccctg taaaactacc
     3661 ctttgaaaac ctctctttct taatcttttc tttgtaattc cagatctatg cccaagaaga
     3721 agaggaaggt ggacaagaag tactccattg ggctcgctat cggcacaaac agcgtcggct
     3781 gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata
     3841 ccgatcgcca cagcataaag aagaacctca ttggcgccct cctgttcgac tccggggaaa
     3901 cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc agaaagaatc
     3961 ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat gactctttct
     4021 tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag cgccacccaa
     4081 tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc atatatcatc
     4141 tgaggaagaa gcttgtagac agtactgata aggctgactt gcggttgatc tatctcgcgc
     4201 tggcgcatat gatcaaattt cggggacact tcctcatcga gggggacctg aacccagaca
     4261 acagcgatgt cgacaaactc tttatccaac tggttcagac ttacaatcag cttttcgaag
     4321 agaacccgat caacgcatcc ggagttgacg ccaaagcaat cctgagcgct aggctgtcca
     4381 aatcccggcg gctcgaaaac ctcatcgcac agctccctgg ggagaagaag aacggcctgt
     4441 ttggtaatct tatcgccctg tcactcgggc tgacccccaa ctttaaatct aacttcgacc
     4501 tggccgaaga tgccaagctt caactgagca aagacaccta cgatgatgat ctcgacaatc
     4561 tgctggccca gatcggcgac cagtacgcag accttttttt ggcggcaaag aacctgtcag
     4621 acgccattct gctgagtgat attctgcgag tgaacacgga gatcaccaaa gctccgctga
     4681 gcgctagtat gatcaagcgc tatgatgagc accaccaaga cttgactttg ctgaaggccc
     4741 ttgtcagaca gcaactgcct gagaagtaca aggaaatttt cttcgatcag tctaaaaatg
     4801 gctacgccgg atacattgac ggcggagcaa gccaggagga attttacaaa tttattaagc
     4861 ccatcttgga aaaaatggac ggcaccgagg agctgctggt aaagcttaac agagaagatc
     4921 tgttgcgcaa acagcgcact ttcgacaatg gaagcatccc ccaccagatt cacctgggcg
     4981 aactgcacgc tatcctcagg cggcaagagg atttctaccc ctttttgaaa gataacaggg
     5041 aaaagattga gaaaatcctc acatttcgga taccctacta tgtaggcccc ctcgcccggg
     5101 gaaattccag attcgcgtgg atgactcgca aatcagaaga gactatcact ccctggaact
     5161 tcgaggaagt cgtggataag ggggcctctg cccagtcctt catcgaaagg atgactaact
     5221 ttgataaaaa tctgcctaac gaaaaggtgc ttcctaaaca ctctctgctg tacgagtact
     5281 tcacagttta taacgagctc accaaggtca aatacgtcac agaagggatg agaaagccag
     5341 cattcctgtc tggagagcag aagaaagcta tcgtggacct cctcttcaag acgaaccgga
     5401 aagttaccgt gaaacagctc aaagaagatt atttcaaaaa gattgaatgt ttcgactctg
     5461 ttgaaatcag cggagtggag gatcgcttca acgcatccct gggaacgtat cacgatctcc
     5521 tgaaaatcat taaagacaag gacttcctgg acaatgagga gaacgaggac attcttgagg
     5581 acattgtcct cacccttacg ttgtttgaag atagggagat gattgaagaa cgcttgaaaa
     5641 cttacgctca tctcttcgac gacaaagtca tgaaacagct caagaggcgc cgatatacag
     5701 gatgggggcg gctgtcaaga aaactgatca atgggatccg agacaagcag agtggaaaga
     5761 caatcctgga ttttcttaag tccgatggat ttgccaaccg gaacttcatg cagttgatcc
     5821 atgatgactc tctcaccttt aaggaggaca tccagaaagc acaagtttct ggccaggggg
     5881 acagtctcca cgagcacatc gctaatcttg caggtagccc agctatcaaa aagggaatac
     5941 tgcagaccgt taaggtcgtg gatgaactcg tcaaagtaat gggaaggcat aagcccgaga
     6001 atatcgttat cgagatggcc cgagagaacc aaactaccca gaagggacag aagaacagta
     6061 gggaaaggat gaagaggatt gaagagggta taaaagaact ggggtcccaa atccttaagg
     6121 aacacccagt tgaaaacacc cagcttcaga atgagaagct ctacctgtac tacctgcaga
     6181 acggcaggga catgtacgtg gatcaggaac tggacatcaa tcggctctcc gactacgacg
     6241 tggatgccat cgtgccccag tcttttctca aagatgattc tattgataat aaagtgttga
     6301 caagatccga taaaaataga gggaagagtg ataacgtccc ctcagaagaa gttgtcaaga
     6361 aaatgaaaaa ttattggcgg cagctgctga acgccaaact gatcacacaa cggaagttcg
     6421 ataatctgac taaggctgaa cgaggtggcc tgtctgagtt ggataaagcc ggcttcatca
     6481 aaaggcagct tgttgagaca cgccagatca ccaagcacgt ggcccaaatt ctcgattcac
     6541 gcatgaacac caagtacgat gaaaatgaca aactgattcg agaggtgaaa gttattactc
     6601 tgaagtctaa gctggtttca gatttcagaa aggactttca gttttataag gtgagagaga
     6661 tcaacaatta ccaccatgcg catgatgcct acctgaatgc agtggtaggc actgcactta
     6721 tcaaaaaata tcccaagctt gaatctgaat ttgtttacgg agactataaa gtgtacgatg
     6781 ttaggaaaat gatcgcaaag tctgagcagg aaataggcaa ggccaccgct aagtacttct
     6841 tttacagcaa tattatgaat tttttcaaga ccgagattac actggccaat ggagagattc
     6901 ggaagcgacc acttatcgaa acaaacggag aaacaggaga aatcgtgtgg gacaagggta
     6961 gggatttcgc gacagtccgg aaggtcctgt ccatgccgca ggtgaacatc gttaaaaaga
     7021 ccgaagtaca gaccggaggc ttctccaagg aaagtatcct cccgaaaagg aacagcgaca
     7081 agctgatcgc acgcaaaaaa gattgggacc ccaagaaata cggcggattc gattctccta
     7141 cagtcgctta cagtgtactg gttgtggcca aagtggagaa agggaagtct aaaaaactca
     7201 aaagcgtcaa ggaactgctg ggcatcacaa tcatggagcg atcaagcttc gaaaaaaacc
     7261 ccatcgactt tctcgaggcg aaaggatata aagaggtcaa aaaagacctc atcattaagc
     7321 ttcccaagta ctctctcttt gagcttgaaa acggccggaa acgaatgctc gctagtgcgg
     7381 gcgagctgca gaaaggtaac gagctggcac tgccctctaa atacgttaat ttcttgtatc
     7441 tggccagcca ctatgaaaag ctcaaaggat ctcccgaaga taatgagcag aagcagctgt
     7501 tcgtggaaca acacaaacac taccttgatg agatcatcga gcaaataagc gaattctcca
     7561 aaagagtgat cctcgccgac gctaacctcg ataaggtgct ttctgcttac aataagcaca
     7621 gggataagcc catcagggag caggcagaaa acattatcca cttgtttact ctgaccaact
     7681 tgggcgcgcc tgcagccttc aagtacttcg acaccaccat agacagaaag cggtacacct
     7741 ctacaaagga ggtcctggac gccacactga ttcatcagtc aattacgggg ctctatgaaa
     7801 caagaatcga cctctctcag ctcggtggag acagcagggc tgattcggac ccaaaaaaga
     7861 agcgtaaggt cgatcccaag aagaagagaa aggtagatcc taagaagaag agaaaggtag
     7921 acgcattgga tgacttcgac ttagacatgc ttgggtcaga tgctttggac gactttgacc
     7981 tcgacatgtt gggttctgac gcgctagatg acttcgactt ggacatgttg ggctccgacg
     8041 ctttggacga ctttgatctg gacatgttat gaggatccta actcgagctc tagctagagt
     8101 cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt
     8161 agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     8221 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact
     8281 aaaatccaga tgctgagcag ttacagagat gttacgaacc gggacacgaa gtggtttaaa
     8341 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     8401 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgcc
     8461 aaccacagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8521 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8581 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8641 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8701 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8761 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8821 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8881 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8941 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9001 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9061 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9121 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9181 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9241 taaacttggt ctgacagcta aaacaattca tccagtaaaa tataatattt tattttctcc
     9301 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
     9361 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
     9421 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
     9481 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
     9541 agctcgcgcg gatctttaaa tggagtgtcc tcttcccagt tttcgcaatc cacatcggcc
     9601 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
     9661 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
     9721 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
     9781 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
     9841 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
     9901 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
     9961 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
    10021 ttcaattccg gtgatattct cattttagcc atactcttcc tttttcaata ttattgaagc
    10081 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10141 caaatagggg ttccgcgcac gaattggcca gcgctgccat ttttggggtg aggccgttcg
    10201 cggccgaggg gcgcagcccc tggggggatg ggagctcggt gccttgatgt gggcgccggc
    10261 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
    10321 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
    10381 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
    10441 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
    10501 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
    10561 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
    10621 acgtgctatc cacaggaaag aagctttttc gacctttttc ccctgctagg gcaatttgcc
    10681 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
    10741 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
    10801 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
    10861 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
    10921 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
    10981 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
    11041 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
    11101 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
    11161 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
    11221 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
    11281 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
    11341 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
    11401 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
    11461 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
    11521 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
    11581 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
    11641 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
    11701 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
    11761 tgcgaccgtc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
    11821 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
    11881 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
    11941 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
    12001 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
    12061 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
    12121 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
    12181 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
    12241 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
    12301 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
    12361 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
    12421 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
    12481 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
    12541 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
    12601 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
    12661 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
    12721 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
    12781 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
    12841 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
    12901 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
    12961 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
    13021 cggctgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
    13081 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
    13141 ataacacatt gcggacgttt ttaatgtact ggggtggatg cagtgggccc cactctgtgt
    13201 gccgaattcg gatccggaga gcggagaatt aagggagtca cgttatgacc cccgccgatg
    13261 acgcgggaca agccgtttta cgtttggaac tgacagaacc gcaacgttga aggagccact
    13321 cagccgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc attattgcgc
    13381 gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa aaatgctcca
    13441 ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac tctcaatcca
    13501 aataatctgc accggatctg gatcgtttcg catgattgaa caagatggat tgcacgcagg
    13561 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
    13621 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
    13681 gaccgacctg tccggtgccc tgaatgaact ccaggacgag gcagcgcggc tatcgtggct
    13741 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
    13801 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
    13861 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
    13921 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
    13981 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
    14041 gttcgccagg ctcaaggcgc gtatgcccga cggcgaggat ctcgtcgtga ctcatggcga
    14101 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
    14161 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
    14221 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    14281 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctggat
    14341 ctagagtcaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
    14401 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
    14461 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
    14521 atacatctaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
    14581 cgcggtgtca tctatgttac tagatcgacg ctgcaagaat tcaagct
//
