LOCUS       OCS_1-5                13685 bp    DNA     circular UNA 04-JUN-2021
DEFINITION  Golden Gate/Type IIS Cloning ligation product.
ACCESSION   urn.local...1s3-dfpu1lk
VERSION     urn.local...1s3-dfpu1lk
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    6..1001
                     /Source="referenceToDocument: urn:local:.:1rn-dfpu0yj"
                     /label="TU1-5 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    6..30
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK002"
                     /Transferred_Similarity="100.00%"
                     /label="ConS scar"
     promoter        36..454
                     /modified_by="User"
                     /label="CaMV 35 S promoter (short)"
     Misc._binding   455..461
                     /label="CaMV 5' UTL"
     Misc._binding   455
                     /label="Start of transcription"
     5'UTR           462..554
                     /modified_by="User"
                     /label="CMV1 5'UTR"
     source          <564..>774
                     /organism="Cloning vector CMVp-HHRibo-gRNA1-HDVRibo-pA"
                     /mol_type="other DNA"
                     /db_xref="taxon:1520530"
                     /label="source Cloning vector
                     CMVp-HHRibo-gRNA1-HDVRibo-pA"
     misc_feature    564..606
                     /note="5_prime HH self-cleaving ribozyme"
                     /label="5 prime HH self-cleaving ribozyme"
     misc_feature    607..626
                     /created_by="User"
                     /label="gRNA-1"
     misc_feature    <627..706
                     /note="gRNA1"
                     /modified_by="User"
                     /label="gRNA handle"
     misc_feature    707..774
                     /note="3_prime HDV self-cleaving ribozyme"
                     /label="3 prime HDV self-cleaving ribozyme"
     terminator      790..993
                     /label="35S ter"
     misc_feature    998..2214
                     /Source="referenceToDocument: urn:local:.:1qi-dfptqs4"
                     /label="TU2-1 (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    998..1022
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK003"
                     /Transferred_Similarity="100.00%"
                     /label="Con1 scar"
     promoter        1028..1161
                     /created_by="User"
                     /modified_by="User"
                     /label="ATF1-2 promoter"
     Misc._binding   1029..1048
                     /created_by="User"
                     /label="gRNA1-target"
     Misc._binding   1058..1077
                     /created_by="User"
                     /label="gRNA1-target"
     Misc._binding   1087..1106
                     /created_by="User"
                     /label="gRNA1-target"
     promoter        <1116..1161
                     /modified_by="User"
                     /label="minimal 35S"
     Misc._binding   1162..1168
                     /label="CaMV 5' UTL"
     Misc._binding   1162
                     /label="Start of transcription"
     5'UTR           1169..1261
                     /modified_by="User"
                     /label="CMV1 5'UTR"
     CDS             1268..1987
                     /created_by="User"
                     /label="YFP"
     terminator      2003..2206
                     /label="35S ter"
     misc_feature    2211..7357
                     /Source="referenceToDocument: urn:local:.:1qt-dfptrdc"
                     /label="TU3E-1 (concatenated sequence 4)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    2215..2235
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK004"
                     /Transferred_Similarity="100.00%"
                     /label="Con2 scar"
     promoter        2241..2659
                     /modified_by="User"
                     /label="CaMV 35 S promoter (short)"
     Misc._binding   2660..2666
                     /label="CaMV 5' UTL"
     Misc._binding   2660
                     /label="Start of transcription"
     5'UTR           2667..2759
                     /modified_by="User"
                     /label="CMV1 5'UTR"
     source          <2766..>7127
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             2769..2789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             2790..6890
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II
                     CRISPR/Cas system"
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC
                     catalytic domain and the H840A mutation in the HNH
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
                     SFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHM
                     IKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKS
                     RRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDN
                     LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLL
                     KALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKL
                     NREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY
                     VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP
                     KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKED
                     YFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTL
                     FEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFL
                     KSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTV
                     KVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEH
                     PVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVL
                     TRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAG
                     FIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFY
                     KVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSM
                     PQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVA
                     KVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFE
                     LENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHK
                     HYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAP
                     AAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /label="dCas9"
     CDS             6909..6929
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             6933..6953
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             6957..6977
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             6978..7127
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDML"
                     /label="VP64"
     terminator      7146..7349
                     /label="35S ter"
     misc_feature    7354..9
                     /Source="referenceToDocument: urn:local:.:1r3-dfptt38"
                     /label="pAPT.Ex.KanR Copy (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    7354..7378
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK073, pYTK096"
                     /Transferred_Similarity="100.00%"
                     /label="ConE scar"
     misc_feature    complement(7396..7523)
                     /label="RB short"
     rep_origin      complement(7542..8156)
                     /modified_by="User"
                     /label="rep"
     rep_origin      7557
                     /label="ORI"
     CDS             complement(8319..9110)
                     /label="Kan (no BpiI)"
     mutation        8628
                     /label="A to G (BpiI removed)"
     rep_origin      complement(9296..12080)
                     /modified_by="User"
                     /label="pVS1ori short"
     misc_feature    12082..12229
                     /label="LB"
     misc_feature    12278..12577
                     /label="nos prom"
                     /note="Geneious type: promoter uukaryotic"
     CDS             12590..13381
                     /modified_by="User"
                     /label="KanR"
     terminator      13399..13666
                     /label="nos term"
ORIGIN      
        1 ctagactgaa ctggccgata attgcagacg aacgcgtcaa catggtggag cacgacactc
       61 tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct attgagactt
      121 ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
      181 tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
      241 gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga cccccaccca
      301 cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa gtggattgat
      361 gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
      421 cctctatata aggaagttca tttcatttgg agaggacacg cgtttattta caagagcgta
      481 cggttcaatc cctgcctccc ctgtaaaact accctttgaa aacctctctt tcttaatctt
      541 ttctttgtaa ttccagatct atgcgactac tgatgagtcc gtgaggacga aacgagtaag
      601 ctcgtcaaac cacaatttgc acacccgttt tagagctaga aatagcaagt taaaataagg
      661 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttggcc ggcatggtcc
      721 cagcctcctc gctggcgccg gctgggcaac atgcttcggc atggcgaatg ggacggatcc
      781 taactcgagc tctagctaga gtcgatcgac aagctcgagt ttctccataa taatgtgtga
      841 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
      901 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
      961 cctaaaacca aaatccagta ctaaaatcca gatgctgcca aaccagatgt caacacagct
     1021 acaacgccaa accacaattt gcacaccctg gcatctcaaa ccacaatttg cacaccctgg
     1081 catctcaaac cacaatttgc acaccctggc atctccgcaa gacccttcct ctatataagg
     1141 aagttcattt catttggaga ggacacgcgt ttatttacaa gagcgtacgg ttcaatccct
     1201 gcctcccctg taaaactacc ctttgaaaac ctctctttct taatcttttc tttgtaattc
     1261 cagatctatg gtatccaaag gagaagaatt gtttacaggg gtagtcccca tattggtcga
     1321 gctcgatggg gatgtaaacg ggcacaagtt ctcagtgtct ggcgaaggcg aaggggacgc
     1381 cacatacgga aagttgactc tgaagttcat ctgcacaacc ggtaaactac ccgtaccctg
     1441 gccaacactg gtgacgacct ttgggtacgg gctacaatgt ttcgctcgat atcctgacca
     1501 catgaagcaa cacgatttct tcaaaagcgc catgcctgag gggtatgtac aggagcgtac
     1561 catatttttc aaagacgatg gaaactataa gacccgagct gaagtgaaat ttgaaggaga
     1621 tacgttagtc aatcgaatag aactaaaggg aattgatttc aaggaggatg ggaatattct
     1681 gggccataag ctggagtaca attacaatag tcacaacgtc tatataatgg cagacaagca
     1741 gaagaatgga ataaaggtta atttcaagat aaggcataac attgaagacg gaagcgttca
     1801 gctagccgat cattaccaac agaatacacc gataggtgat ggacccgtcc tgctccccga
     1861 caaccattac ttgtcttacc agagtgcact ttctaaagat ccaaatgaga aaagagatca
     1921 tatggtttta ttagaatttg ttaccgcagc cggcataact ttgggaatgg acgaactgta
     1981 caaatgagga tcctaactcg agctctagct agagtcgatc gacaagctcg agtttctcca
     2041 taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat
     2101 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     2161 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatgctg gatgcacaca
     2221 ctggcttaag atgacaacgc gtcaacatgg tggagcacga cactctggtc tactccaaaa
     2281 atgtcaaaga tacagtctca gaagatcaaa gggctattga gacttttcaa caaaggataa
     2341 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
     2401 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
     2461 aagatctctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     2521 aaaaagaaga ggttccaacc acgtctacaa agcaagtgga ttgatgtgac atctccactg
     2581 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     2641 gttcatttca tttggagagg acacgcgttt atttacaaga gcgtacggtt caatccctgc
     2701 ctcccctgta aaactaccct ttgaaaacct ctctttctta atcttttctt tgtaattcca
     2761 gatctatgcc caagaagaag aggaaggtgg acaagaagta ctccattggg ctcgctatcg
     2821 gcacaaacag cgtcggctgg gccgtcatta cggacgagta caaggtgccg agcaaaaaat
     2881 tcaaagttct gggcaatacc gatcgccaca gcataaagaa gaacctcatt ggcgccctcc
     2941 tgttcgactc cggggaaacg gccgaagcca cgcggctcaa aagaacagca cggcgcagat
     3001 atacccgcag aaagaatcgg atctgctacc tgcaggagat ctttagtaat gagatggcta
     3061 aggtggatga ctctttcttc cataggctgg aggagtcctt tttggtggag gaggataaaa
     3121 agcacgagcg ccacccaatc tttggcaata tcgtggacga ggtggcgtac catgaaaagt
     3181 acccaaccat atatcatctg aggaagaagc ttgtagacag tactgataag gctgacttgc
     3241 ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg gggacacttc ctcatcgagg
     3301 gggacctgaa cccagacaac agcgatgtcg acaaactctt tatccaactg gttcagactt
     3361 acaatcagct tttcgaagag aacccgatca acgcatccgg agttgacgcc aaagcaatcc
     3421 tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct catcgcacag ctccctgggg
     3481 agaagaagaa cggcctgttt ggtaatctta tcgccctgtc actcgggctg acccccaact
     3541 ttaaatctaa cttcgacctg gccgaagatg ccaagcttca actgagcaaa gacacctacg
     3601 atgatgatct cgacaatctg ctggcccaga tcggcgacca gtacgcagac ctttttttgg
     3661 cggcaaagaa cctgtcagac gccattctgc tgagtgatat tctgcgagtg aacacggaga
     3721 tcaccaaagc tccgctgagc gctagtatga tcaagcgcta tgatgagcac caccaagact
     3781 tgactttgct gaaggccctt gtcagacagc aactgcctga gaagtacaag gaaattttct
     3841 tcgatcagtc taaaaatggc tacgccggat acattgacgg cggagcaagc caggaggaat
     3901 tttacaaatt tattaagccc atcttggaaa aaatggacgg caccgaggag ctgctggtaa
     3961 agcttaacag agaagatctg ttgcgcaaac agcgcacttt cgacaatgga agcatccccc
     4021 accagattca cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat ttctacccct
     4081 ttttgaaaga taacagggaa aagattgaga aaatcctcac atttcggata ccctactatg
     4141 taggccccct cgcccgggga aattccagat tcgcgtggat gactcgcaaa tcagaagaga
     4201 ctatcactcc ctggaacttc gaggaagtcg tggataaggg ggcctctgcc cagtccttca
     4261 tcgaaaggat gactaacttt gataaaaatc tgcctaacga aaaggtgctt cctaaacact
     4321 ctctgctgta cgagtacttc acagtttata acgagctcac caaggtcaaa tacgtcacag
     4381 aagggatgag aaagccagca ttcctgtctg gagagcagaa gaaagctatc gtggacctcc
     4441 tcttcaagac gaaccggaaa gttaccgtga aacagctcaa agaagattat ttcaaaaaga
     4501 ttgaatgttt cgactctgtt gaaatcagcg gagtggagga tcgcttcaac gcatccctgg
     4561 gaacgtatca cgatctcctg aaaatcatta aagacaagga cttcctggac aatgaggaga
     4621 acgaggacat tcttgaggac attgtcctca cccttacgtt gtttgaagat agggagatga
     4681 ttgaagaacg cttgaaaact tacgctcatc tcttcgacga caaagtcatg aaacagctca
     4741 agaggcgccg atatacagga tgggggcggc tgtcaagaaa actgatcaat gggatccgag
     4801 acaagcagag tggaaagaca atcctggatt ttcttaagtc cgatggattt gccaaccgga
     4861 acttcatgca gttgatccat gatgactctc tcacctttaa ggaggacatc cagaaagcac
     4921 aagtttctgg ccagggggac agtctccacg agcacatcgc taatcttgca ggtagcccag
     4981 ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga tgaactcgtc aaagtaatgg
     5041 gaaggcataa gcccgagaat atcgttatcg agatggcccg agagaaccaa actacccaga
     5101 agggacagaa gaacagtagg gaaaggatga agaggattga agagggtata aaagaactgg
     5161 ggtcccaaat ccttaaggaa cacccagttg aaaacaccca gcttcagaat gagaagctct
     5221 acctgtacta cctgcagaac ggcagggaca tgtacgtgga tcaggaactg gacatcaatc
     5281 ggctctccga ctacgacgtg gatgccatcg tgccccagtc ttttctcaaa gatgattcta
     5341 ttgataataa agtgttgaca agatccgata aaaatagagg gaagagtgat aacgtcccct
     5401 cagaagaagt tgtcaagaaa atgaaaaatt attggcggca gctgctgaac gccaaactga
     5461 tcacacaacg gaagttcgat aatctgacta aggctgaacg aggtggcctg tctgagttgg
     5521 ataaagccgg cttcatcaaa aggcagcttg ttgagacacg ccagatcacc aagcacgtgg
     5581 cccaaattct cgattcacgc atgaacacca agtacgatga aaatgacaaa ctgattcgag
     5641 aggtgaaagt tattactctg aagtctaagc tggtttcaga tttcagaaag gactttcagt
     5701 tttataaggt gagagagatc aacaattacc accatgcgca tgatgcctac ctgaatgcag
     5761 tggtaggcac tgcacttatc aaaaaatatc ccaagcttga atctgaattt gtttacggag
     5821 actataaagt gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa ataggcaagg
     5881 ccaccgctaa gtacttcttt tacagcaata ttatgaattt tttcaagacc gagattacac
     5941 tggccaatgg agagattcgg aagcgaccac ttatcgaaac aaacggagaa acaggagaaa
     6001 tcgtgtggga caagggtagg gatttcgcga cagtccggaa ggtcctgtcc atgccgcagg
     6061 tgaacatcgt taaaaagacc gaagtacaga ccggaggctt ctccaaggaa agtatcctcc
     6121 cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc aagaaatacg
     6181 gcggattcga ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa gtggagaaag
     6241 ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg catcacaatc atggagcgat
     6301 caagcttcga aaaaaacccc atcgactttc tcgaggcgaa aggatataaa gaggtcaaaa
     6361 aagacctcat cattaagctt cccaagtact ctctctttga gcttgaaaac ggccggaaac
     6421 gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga gctggcactg ccctctaaat
     6481 acgttaattt cttgtatctg gccagccact atgaaaagct caaaggatct cccgaagata
     6541 atgagcagaa gcagctgttc gtggaacaac acaaacacta ccttgatgag atcatcgagc
     6601 aaataagcga attctccaaa agagtgatcc tcgccgacgc taacctcgat aaggtgcttt
     6661 ctgcttacaa taagcacagg gataagccca tcagggagca ggcagaaaac attatccact
     6721 tgtttactct gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac accaccatag
     6781 acagaaagcg gtacacctct acaaaggagg tcctggacgc cacactgatt catcagtcaa
     6841 ttacggggct ctatgaaaca agaatcgacc tctctcagct cggtggagac agcagggctg
     6901 attcggaccc aaaaaagaag cgtaaggtcg atcccaagaa gaagagaaag gtagatccta
     6961 agaagaagag aaaggtagac gcattggatg acttcgactt agacatgctt gggtcagatg
     7021 ctttggacga ctttgacctc gacatgttgg gttctgacgc gctagatgac ttcgacttgg
     7081 acatgttggg ctccgacgct ttggacgact ttgatctgga catgttatga ggatcctaac
     7141 tcgagctcta gctagagtcg atcgacaagc tcgagtttct ccataataat gtgtgagtag
     7201 ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga
     7261 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
     7321 aaaccaaaat ccagtactaa aatccagatg ctgagcagtt acagagatgt tacgaaccgg
     7381 gacacgaagt ggtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
     7441 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     7501 cgtccatttg tatgtgccaa ccacaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7561 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7621 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7681 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7741 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7801 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7861 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7921 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7981 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8041 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8101 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8161 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8221 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8281 atctaaagta tatatgagta aacttggtct gacagctaaa acaattcatc cagtaaaata
     8341 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
     8401 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
     8461 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
     8521 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
     8581 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcctc ttcccagttt
     8641 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
     8701 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
     8761 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
     8821 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
     8881 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
     8941 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
     9001 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
     9061 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat actcttcctt
     9121 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9181 tgtatttaga aaaataaaca aataggggtt ccgcgcacga attggccagc gctgccattt
     9241 ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg agctcggtgc
     9301 cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat
     9361 tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag
     9421 cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg
     9481 tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta
     9541 aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg
     9601 accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg
     9661 gctttgggtt cccaatgtac gtgctatcca caggaaagaa gctttttcga cctttttccc
     9721 ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc
     9781 cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct
     9841 tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact
     9901 tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt
     9961 ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga
    10021 tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc
    10081 ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta
    10141 cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct
    10201 tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta
    10261 ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa
    10321 cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg
    10381 attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc
    10441 cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc
    10501 cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat
    10561 cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg
    10621 gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc
    10681 gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct
    10741 gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt
    10801 gtaccgggcc ggatggtttg cgaccgtcac gccgattcct cgggcttggg ggttccagtg
    10861 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
    10921 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
    10981 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
    11041 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
    11101 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
    11161 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
    11221 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
    11281 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
    11341 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
    11401 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
    11461 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
    11521 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
    11581 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
    11641 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
    11701 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
    11761 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
    11821 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
    11881 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
    11941 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
    12001 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
    12061 ggatttcatg cagccgcacg gctgatgggc tgcctgtatc gagtggtgat tttgtgccga
    12121 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
    12181 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggatgca
    12241 gtgggcccca ctctgtgtgc cgaattcgga tccggagagc ggagaattaa gggagtcacg
    12301 ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc
    12361 aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa gcacatacgt
    12421 cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt
    12481 cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc
    12541 atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca tgattgaaca
    12601 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
    12661 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
    12721 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aggacgaggc
    12781 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
    12841 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
    12901 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
    12961 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
    13021 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
    13081 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgt atgcccgacg gcgaggatct
    13141 cgtcgtgact catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
    13201 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
    13261 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
    13321 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
    13381 ctgagcggga ctctggatct agagtcaagc agatcgttca aacatttggc aataaagttt
    13441 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
    13501 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
    13561 gattagagtc ccgcaattat acatctaata cgcgatagaa aacaaaatat agcgcgcaaa
    13621 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgacgct gcaagaattc
    13681 aagct
//
