LOCUS pAPT.Ex.KanR_Copy 7675 bp DNA circular UNA 29-DEC-2020 DEFINITION . ACCESSION urn.local...1r3-dfptt38 VERSION urn.local...1r3-dfptt38 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers misc_feature complement(11..16) /Recognition_pattern="CGTCTC(1/5)" /annotation_group="CGTCTC" /label="BsmBI" /note="Geneious type: restriction site" misc_feature 17..159 /ApEinfo_fwdcolor="#FFFD6C" /ApEinfo_revcolor="#FFFD6C" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /Transferred_From="pYTK002, pYTK008, pYTK096" /Transferred_Similarity="100.00%" /label="ConS" ligation 160..163 /label="Ligation" misc_feature complement(165..170) /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" promoter 171..300 /ApEinfo_fwdcolor="#9EFF91" /ApEinfo_revcolor="#9EFF91" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /Transferred_From="pYTK001, pYTK047, pYTK050, pYTK095, pYTK096" /Transferred_Similarity="100.00%" /label="BBa_J72163 GlpT Promoter" rbs 301..336 /ApEinfo_fwdcolor="#9EFF91" /ApEinfo_revcolor="#9EFF91" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /Transferred_From="pYTK001, pYTK047, pYTK050, pYTK095, pYTK096" /Transferred_Similarity="100.00%" /label="sfGFP Ribosome Binding Site" cds 337..1053 /ApEinfo_fwdcolor="#34FF03" /ApEinfo_revcolor="#34FF03" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /Transferred_From="pYTK001, pYTK047, pYTK050, pYTK095, pYTK096" /Transferred_Similarity="100.00%" /label="sfGFP" misc_feature 400..405 /Recognition_pattern="GTY^RAC" /annotation_group="GTYRAC" /label="HincII" /note="Geneious type: restriction site" misc_feature 865..870 /Recognition_pattern="CAG^CTG" /annotation_group="CAGCTG" /label="PvuII" /note="Geneious type: restriction site" terminator 1054..1182 /ApEinfo_fwdcolor="#9EFF91" /ApEinfo_revcolor="#9EFF91" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /Transferred_From="pYTK001, pYTK047, pYTK050, pYTK083, pYTK084, pYTK085, pYTK089, pYTK090, pYTK091, pYTK095, pYTK096" /Transferred_Similarity="100.00%" /label="BBa_B0015 Terminator" terminator 1149..1168 /Imported_from="Pla sMapper" /Transferred_From="stem_loop_early_T7 term" /Transferred_Similarity="100.00%" /label="stem_loop_early_T7 term" misc_feature 1183..1188 /Recognition_pattern="GGTCTC(1/5)" /annotation_group="GGTCTC" /label="BsaI" /note="Geneious type: restriction site" misc_feature 1188..1193 /Recognition_pattern="CAG^CTG" /annotation_group="CAGCTG" /label="PvuII" /note="Geneious type: restriction site" ligation 1190..1193 /label="Ligation" misc_feature 1194..1336 /ApEinfo_fwdcolor="#FFFD6C" /ApEinfo_revcolor="#FFFD6C" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /Transferred_From="pYTK072, pYTK073, pYTK096" /Transferred_Similarity="100.00%" /label="ConE" primer complement(1219..1247) /created_by="User" /label="SK.ESseq13.R" misc_feature 1294..1299 /Recognition_pattern="GCC^GGC" /annotation_group="GCCGGC" /label="NaeI" /note="Geneious type: restriction site" misc_feature 1337..1342 /Recognition_pattern="CGTCTC(1/5)" /annotation_group="CGTCTC" /label="BsmBI" /note="Geneious type: restriction site" misc_feature 1344..1368 /ApEinfo_fwdcolor="#FFFD6C" /ApEinfo_revcolor="#FFFD6C" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /Transferred_From="pYTK073, pYTK096" /Transferred_Similarity="100.00%" /label="ConE scar" misc_feature 1373..1381 /Recognition_pattern="CACNNN^GTG" /label="DraIII" /note="Geneious type: restriction site" misc_feature complement(1386..1513) /label="RB short" rep_origin complement(1532..2146) /modified_by="User" /label="rep" rep_origin 1547 /label="ORI" CDS complement(2309..3100) /label="Kan (no BpiI)" mutation 2618 /label="A to G (BpiI removed)" rep_origin complement(3286..6070) /modified_by="User" /label="pVS1ori short" misc_feature 3386..3393 /Recognition_pattern="GC^GGCCGC" /Methylation="CpG:variable" /annotation_group="GCGGCCGC" /label="NotI" /note="Geneious type: restriction site" misc_feature 3459..3465 /Recognition_pattern="GCTCTTC(1/4)" /label="SapI" /note="Geneious type: restriction site" misc_feature 4676..4683 /Recognition_pattern="GC^GGCCGC" /Methylation="CpG:variable" /annotation_group="GCGGCCGC" /label="NotI" /note="Geneious type: restriction site" misc_feature 6072..6219 /label="LB" misc_feature 6239..6247 /Recognition_pattern="CACNNN^GTG" /label="DraIII" /note="Geneious type: restriction site" misc_feature 6268..6567 /label="nos prom" /note="Geneious type: promoter uukaryotic" CDS 6580..7371 /modified_by="User" /label="KanR" misc_feature complement(7057..7063) /Recognition_pattern="GCTCTTC(1/4)" /label="SapI" /note="Geneious type: restriction site" misc_feature complement(7267..7273) /Recognition_pattern="GCTCTTC(1/4)" /label="SapI" /note="Geneious type: restriction site" terminator 7389..7656 /label="nos term" ORIGIN 1 ctagactgat gagacgtggt agagccacaa acagccggta caagcaacga tctccaggac 61 catctgaatc atgcgcggat gacacgaact cacgacggcg atcacagaca ttaacccaca 121 gtacagacac tgcgacaacg tggcaattcg tcgcaataca acgtgagacc gaaagtgaaa 181 cgtgatttca tgcgtcattt tgaacatttt gtaaatctta tttaataatg tgtgcggcaa 241 ttcacattta atttatgaat gttttcttaa catcgcggca actcaagaaa cggcaggttc 301 ggatcttagc tactagagaa agaggagaaa tactagatgc gtaaaggcga agagctgttc 361 actggtgtcg tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc 421 gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt 481 actactggta aactgccggt tccttggccg actctggtaa cgacgctgac ttatggtgtt 541 cagtgctttg ctcgttatcc ggaccatatg aagcagcatg acttcttcaa gtccgccatg 601 ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg 661 cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt 721 gactttaaag aggacggcaa tatcctgggc cataagctgg aatacaattt taacagccac 781 aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc 841 cataacgttg aggatggcag cgtgcagctg gctgatcact accagcaaaa cactccaatc 901 ggtgatggtc ctgttctgct gccagacaat cactatctga gcacgcaaag cgttctgtct 961 aaagatccga acgagaaacg cgatcatatg gttctgctgg agttcgtaac cgcagcgggc 1021 atcacgcatg gtatggatga actgtacaaa tgaccaggca tcaaataaaa cgaaaggctc 1081 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 1141 gtcacactgg cttaccttcg ggtgggcctt tctgcgttta taggtctcag ctggaaatct 1201 gctcgtcagt ggtgctcaca ctgacgaatc atgtacagat cataccgatg actgcctggc 1261 gactcacaac taagcaagac agccggaacc agcgccggcg aacaccactg catatatggc 1321 atatcacaac agtccacgtc tcaagcagtt acagagatgt tacgaaccgg gacacgaagt 1381 ggtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg 1441 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg 1501 tatgtgccaa ccacaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 1561 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 1621 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 1681 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 1741 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 1801 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 1861 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 1921 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 1981 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 2041 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 2101 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 2161 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 2221 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 2281 tatatgagta aacttggtct gacagctaaa acaattcatc cagtaaaata taatatttta 2341 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt 2401 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc 2461 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc 2521 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa 2581 aatcatacag ctcgcgcgga tctttaaatg gagtgtcctc ttcccagttt tcgcaatcca 2641 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat 2701 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca 2761 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat 2821 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg 2881 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg 2941 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca 3001 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga 3061 tcagtttttt caattccggt gatattctca ttttagccat actcttcctt tttcaatatt 3121 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 3181 aaaataaaca aataggggtt ccgcgcacga attggccagc gctgccattt ttggggtgag 3241 gccgttcgcg gccgaggggc gcagcccctg gggggatggg agctcggtgc cttgatgtgg 3301 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg 3361 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg 3421 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc 3481 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta 3541 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc 3601 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt 3661 cccaatgtac gtgctatcca caggaaagaa gctttttcga cctttttccc ctgctagggc 3721 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag 3781 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta 3841 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc 3901 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc 3961 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta 4021 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca 4081 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta 4141 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt 4201 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc 4261 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg 4321 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag 4381 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc 4441 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg 4501 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc 4561 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct 4621 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc 4681 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg 4741 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc 4801 ggatggtttg cgaccgtcac gccgattcct cgggcttggg ggttccagtg ccattgcagg 4861 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc 4921 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta 4981 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca 5041 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga 5101 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc 5161 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt 5221 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca 5281 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg 5341 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct 5401 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg 5461 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct 5521 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga 5581 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca 5641 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc 5701 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg 5761 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg 5821 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc 5881 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg 5941 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc 6001 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg 6061 cagccgcacg gctgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc 6121 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag 6181 acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggatgca gtgggcccca 6241 ctctgtgtgc cgaattcgga tccggagagc ggagaattaa gggagtcacg ttatgacccc 6301 cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag 6361 gagccactca gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat 6421 tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa 6481 atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc 6541 tcaatccaaa taatctgcac cggatctgga tcgtttcgca tgattgaaca agatggattg 6601 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 6661 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 6721 tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aggacgaggc agcgcggcta 6781 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 6841 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 6901 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 6961 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 7021 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 7081 gccgaactgt tcgccaggct caaggcgcgt atgcccgacg gcgaggatct cgtcgtgact 7141 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 7201 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 7261 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 7321 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga 7381 ctctggatct agagtcaagc agatcgttca aacatttggc aataaagttt cttaagattg 7441 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat 7501 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc 7561 ccgcaattat acatctaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa 7621 ttatcgcgcg cggtgtcatc tatgttacta gatcgacgct gcaagaattc aagct //