LOCUS       pAPT.Ex.KanR_Copy        7675 bp    DNA     circular UNA 29-DEC-2020
DEFINITION  .
ACCESSION   urn.local...1r3-dfptt38
VERSION     urn.local...1r3-dfptt38
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    complement(11..16)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     misc_feature    17..159
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK002, pYTK008, pYTK096"
                     /Transferred_Similarity="100.00%"
                     /label="ConS"
     ligation        160..163
                     /label="Ligation"
     misc_feature    complement(165..170)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     promoter        171..300
                     /ApEinfo_fwdcolor="#9EFF91"
                     /ApEinfo_revcolor="#9EFF91"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK001, pYTK047, pYTK050, pYTK095,
                     pYTK096"
                     /Transferred_Similarity="100.00%"
                     /label="BBa_J72163 GlpT Promoter"
     rbs             301..336
                     /ApEinfo_fwdcolor="#9EFF91"
                     /ApEinfo_revcolor="#9EFF91"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK001, pYTK047, pYTK050, pYTK095,
                     pYTK096"
                     /Transferred_Similarity="100.00%"
                     /label="sfGFP Ribosome Binding Site"
     cds             337..1053
                     /ApEinfo_fwdcolor="#34FF03"
                     /ApEinfo_revcolor="#34FF03"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK001, pYTK047, pYTK050, pYTK095,
                     pYTK096"
                     /Transferred_Similarity="100.00%"
                     /label="sfGFP"
     misc_feature    400..405
                     /Recognition_pattern="GTY^RAC"
                     /annotation_group="GTYRAC"
                     /label="HincII"
                     /note="Geneious type: restriction site"
     misc_feature    865..870
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     terminator      1054..1182
                     /ApEinfo_fwdcolor="#9EFF91"
                     /ApEinfo_revcolor="#9EFF91"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK001, pYTK047, pYTK050, pYTK083,
                     pYTK084, pYTK085, pYTK089, pYTK090, pYTK091, pYTK095,
                     pYTK096"
                     /Transferred_Similarity="100.00%"
                     /label="BBa_B0015 Terminator"
     terminator      1149..1168
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="stem_loop_early_T7 term"
                     /Transferred_Similarity="100.00%"
                     /label="stem_loop_early_T7 term"
     misc_feature    1183..1188
                     /Recognition_pattern="GGTCTC(1/5)"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    1188..1193
                     /Recognition_pattern="CAG^CTG"
                     /annotation_group="CAGCTG"
                     /label="PvuII"
                     /note="Geneious type: restriction site"
     ligation        1190..1193
                     /label="Ligation"
     misc_feature    1194..1336
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK072, pYTK073, pYTK096"
                     /Transferred_Similarity="100.00%"
                     /label="ConE"
     primer          complement(1219..1247)
                     /created_by="User"
                     /label="SK.ESseq13.R"
     misc_feature    1294..1299
                     /Recognition_pattern="GCC^GGC"
                     /annotation_group="GCCGGC"
                     /label="NaeI"
                     /note="Geneious type: restriction site"
     misc_feature    1337..1342
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     misc_feature    1344..1368
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK073, pYTK096"
                     /Transferred_Similarity="100.00%"
                     /label="ConE scar"
     misc_feature    1373..1381
                     /Recognition_pattern="CACNNN^GTG"
                     /label="DraIII"
                     /note="Geneious type: restriction site"
     misc_feature    complement(1386..1513)
                     /label="RB short"
     rep_origin      complement(1532..2146)
                     /modified_by="User"
                     /label="rep"
     rep_origin      1547
                     /label="ORI"
     CDS             complement(2309..3100)
                     /label="Kan (no BpiI)"
     mutation        2618
                     /label="A to G (BpiI removed)"
     rep_origin      complement(3286..6070)
                     /modified_by="User"
                     /label="pVS1ori short"
     misc_feature    3386..3393
                     /Recognition_pattern="GC^GGCCGC"
                     /Methylation="CpG:variable"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     misc_feature    3459..3465
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /label="SapI"
                     /note="Geneious type: restriction site"
     misc_feature    4676..4683
                     /Recognition_pattern="GC^GGCCGC"
                     /Methylation="CpG:variable"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     misc_feature    6072..6219
                     /label="LB"
     misc_feature    6239..6247
                     /Recognition_pattern="CACNNN^GTG"
                     /label="DraIII"
                     /note="Geneious type: restriction site"
     misc_feature    6268..6567
                     /label="nos prom"
                     /note="Geneious type: promoter uukaryotic"
     CDS             6580..7371
                     /modified_by="User"
                     /label="KanR"
     misc_feature    complement(7057..7063)
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /label="SapI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(7267..7273)
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /label="SapI"
                     /note="Geneious type: restriction site"
     terminator      7389..7656
                     /label="nos term"
ORIGIN      
        1 ctagactgat gagacgtggt agagccacaa acagccggta caagcaacga tctccaggac
       61 catctgaatc atgcgcggat gacacgaact cacgacggcg atcacagaca ttaacccaca
      121 gtacagacac tgcgacaacg tggcaattcg tcgcaataca acgtgagacc gaaagtgaaa
      181 cgtgatttca tgcgtcattt tgaacatttt gtaaatctta tttaataatg tgtgcggcaa
      241 ttcacattta atttatgaat gttttcttaa catcgcggca actcaagaaa cggcaggttc
      301 ggatcttagc tactagagaa agaggagaaa tactagatgc gtaaaggcga agagctgttc
      361 actggtgtcg tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc
      421 gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt
      481 actactggta aactgccggt tccttggccg actctggtaa cgacgctgac ttatggtgtt
      541 cagtgctttg ctcgttatcc ggaccatatg aagcagcatg acttcttcaa gtccgccatg
      601 ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg
      661 cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt
      721 gactttaaag aggacggcaa tatcctgggc cataagctgg aatacaattt taacagccac
      781 aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc
      841 cataacgttg aggatggcag cgtgcagctg gctgatcact accagcaaaa cactccaatc
      901 ggtgatggtc ctgttctgct gccagacaat cactatctga gcacgcaaag cgttctgtct
      961 aaagatccga acgagaaacg cgatcatatg gttctgctgg agttcgtaac cgcagcgggc
     1021 atcacgcatg gtatggatga actgtacaaa tgaccaggca tcaaataaaa cgaaaggctc
     1081 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1141 gtcacactgg cttaccttcg ggtgggcctt tctgcgttta taggtctcag ctggaaatct
     1201 gctcgtcagt ggtgctcaca ctgacgaatc atgtacagat cataccgatg actgcctggc
     1261 gactcacaac taagcaagac agccggaacc agcgccggcg aacaccactg catatatggc
     1321 atatcacaac agtccacgtc tcaagcagtt acagagatgt tacgaaccgg gacacgaagt
     1381 ggtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     1441 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     1501 tatgtgccaa ccacaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1561 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1621 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1681 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1741 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1801 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1861 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1921 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1981 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2041 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2101 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2161 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2221 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2281 tatatgagta aacttggtct gacagctaaa acaattcatc cagtaaaata taatatttta
     2341 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
     2401 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
     2461 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
     2521 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
     2581 aatcatacag ctcgcgcgga tctttaaatg gagtgtcctc ttcccagttt tcgcaatcca
     2641 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
     2701 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
     2761 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
     2821 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
     2881 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
     2941 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
     3001 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
     3061 tcagtttttt caattccggt gatattctca ttttagccat actcttcctt tttcaatatt
     3121 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3181 aaaataaaca aataggggtt ccgcgcacga attggccagc gctgccattt ttggggtgag
     3241 gccgttcgcg gccgaggggc gcagcccctg gggggatggg agctcggtgc cttgatgtgg
     3301 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
     3361 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
     3421 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
     3481 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
     3541 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
     3601 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
     3661 cccaatgtac gtgctatcca caggaaagaa gctttttcga cctttttccc ctgctagggc
     3721 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
     3781 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
     3841 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
     3901 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
     3961 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
     4021 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
     4081 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
     4141 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
     4201 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
     4261 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
     4321 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
     4381 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
     4441 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
     4501 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
     4561 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
     4621 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
     4681 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
     4741 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
     4801 ggatggtttg cgaccgtcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
     4861 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
     4921 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
     4981 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
     5041 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
     5101 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
     5161 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
     5221 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
     5281 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
     5341 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
     5401 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
     5461 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
     5521 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
     5581 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
     5641 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
     5701 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
     5761 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
     5821 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
     5881 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
     5941 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
     6001 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
     6061 cagccgcacg gctgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
     6121 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
     6181 acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggatgca gtgggcccca
     6241 ctctgtgtgc cgaattcgga tccggagagc ggagaattaa gggagtcacg ttatgacccc
     6301 cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag
     6361 gagccactca gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat
     6421 tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa
     6481 atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc
     6541 tcaatccaaa taatctgcac cggatctgga tcgtttcgca tgattgaaca agatggattg
     6601 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     6661 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     6721 tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aggacgaggc agcgcggcta
     6781 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     6841 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     6901 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     6961 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     7021 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     7081 gccgaactgt tcgccaggct caaggcgcgt atgcccgacg gcgaggatct cgtcgtgact
     7141 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     7201 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     7261 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     7321 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     7381 ctctggatct agagtcaagc agatcgttca aacatttggc aataaagttt cttaagattg
     7441 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat
     7501 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc
     7561 ccgcaattat acatctaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa
     7621 ttatcgcgcg cggtgtcatc tatgttacta gatcgacgct gcaagaattc aagct
//
