LOCUS       OCS_1-9                13592 bp    DNA     circular UNA 04-JUN-2021
DEFINITION  Golden Gate/Type IIS Cloning ligation product.
ACCESSION   urn.local...1sr-dfpu69x
VERSION     urn.local...1sr-dfpu69x
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    6..908
                     /Source="referenceToDocument: urn:local:.:1sb-dfpu5b7"
                     /label="TU1-6 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    6..30
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK002"
                     /Transferred_Similarity="100.00%"
                     /label="ConS scar"
     promoter        55..229
                     /label="5xEBS"
     promoter        55..89
                     /label="EBS"
     promoter        90..124
                     /label="EBS"
     promoter        125..159
                     /label="EBS"
     promoter        160..194
                     /label="EBS"
     promoter        195..229
                     /label="EBS"
     promoter        230..289
                     /label="EBSpro_minimum35S"
     source          <471..>681
                     /organism="Cloning vector CMVp-HHRibo-gRNA1-HDVRibo-pA"
                     /mol_type="other DNA"
                     /db_xref="taxon:1520530"
                     /label="source Cloning vector
                     CMVp-HHRibo-gRNA1-HDVRibo-pA"
     misc_feature    471..513
                     /note="5_prime HH self-cleaving ribozyme"
                     /label="5 prime HH self-cleaving ribozyme"
     misc_feature    514..533
                     /created_by="User"
                     /label="gRNA-1"
     misc_feature    <534..613
                     /note="gRNA1"
                     /modified_by="User"
                     /label="gRNA handle"
     misc_feature    614..681
                     /note="3_prime HDV self-cleaving ribozyme"
                     /label="3 prime HDV self-cleaving ribozyme"
     terminator      697..900
                     /label="35S ter"
     misc_feature    905..2121
                     /Source="referenceToDocument: urn:local:.:1qi-dfptqs4"
                     /label="TU2-1 (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    905..929
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK003"
                     /Transferred_Similarity="100.00%"
                     /label="Con1 scar"
     promoter        935..1068
                     /created_by="User"
                     /modified_by="User"
                     /label="ATF1-2 promoter"
     Misc._binding   936..955
                     /created_by="User"
                     /label="gRNA1-target"
     Misc._binding   965..984
                     /created_by="User"
                     /label="gRNA1-target"
     Misc._binding   994..1013
                     /created_by="User"
                     /label="gRNA1-target"
     promoter        <1023..1068
                     /modified_by="User"
                     /label="minimal 35S"
     Misc._binding   1069..1075
                     /label="CaMV 5' UTL"
     Misc._binding   1069
                     /label="Start of transcription"
     5'UTR           1076..1168
                     /modified_by="User"
                     /label="CMV1 5'UTR"
     CDS             1175..1894
                     /created_by="User"
                     /label="YFP"
     terminator      1910..2113
                     /label="35S ter"
     misc_feature    2118..7264
                     /Source="referenceToDocument: urn:local:.:1qt-dfptrdc"
                     /label="TU3E-1 (concatenated sequence 4)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    2122..2142
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK004"
                     /Transferred_Similarity="100.00%"
                     /label="Con2 scar"
     promoter        2148..2566
                     /modified_by="User"
                     /label="CaMV 35 S promoter (short)"
     Misc._binding   2567..2573
                     /label="CaMV 5' UTL"
     Misc._binding   2567
                     /label="Start of transcription"
     5'UTR           2574..2666
                     /modified_by="User"
                     /label="CMV1 5'UTR"
     source          <2673..>7034
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             2676..2696
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             2697..6797
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II
                     CRISPR/Cas system"
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC
                     catalytic domain and the H840A mutation in the HNH
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
                     SFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHM
                     IKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKS
                     RRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDN
                     LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLL
                     KALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKL
                     NREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY
                     VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP
                     KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKED
                     YFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTL
                     FEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFL
                     KSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTV
                     KVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEH
                     PVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVL
                     TRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAG
                     FIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFY
                     KVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSM
                     PQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVA
                     KVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFE
                     LENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHK
                     HYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAP
                     AAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /label="dCas9"
     CDS             6816..6836
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             6840..6860
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             6864..6884
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             6885..7034
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDML"
                     /label="VP64"
     terminator      7053..7256
                     /label="35S ter"
     misc_feature    7261..9
                     /Source="referenceToDocument: urn:local:.:1r3-dfptt38"
                     /label="pAPT.Ex.KanR Copy (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    7261..7285
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK073, pYTK096"
                     /Transferred_Similarity="100.00%"
                     /label="ConE scar"
     misc_feature    complement(7303..7430)
                     /label="RB short"
     rep_origin      complement(7449..8063)
                     /modified_by="User"
                     /label="rep"
     rep_origin      7464
                     /label="ORI"
     CDS             complement(8226..9017)
                     /label="Kan (no BpiI)"
     mutation        8535
                     /label="A to G (BpiI removed)"
     rep_origin      complement(9203..11987)
                     /modified_by="User"
                     /label="pVS1ori short"
     misc_feature    11989..12136
                     /label="LB"
     misc_feature    12185..12484
                     /label="nos prom"
                     /note="Geneious type: promoter uukaryotic"
     CDS             12497..13288
                     /modified_by="User"
                     /label="KanR"
     terminator      13306..13573
                     /label="nos term"
ORIGIN      
        1 ctagactgaa ctggccgata attgcagacg aacgcattac gaattcccgg ggatcctcat
       61 gatcaaaggg gggatgcact atttaagatc ctcatgatca aaggggggat gcactattta
      121 agatcctcat gatcaaaggg gggatgcact atttaagatc ctcatgatca aaggggggat
      181 gcactattta agatcctcat gatcaaaggg gggatgcact atttaagatc cttcgcaaga
      241 cccttcctct atataaggaa gttcatttca tttggagagg acagggtatc aagcttggca
      301 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      361 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      421 ccttcccaac agttgcgcag cctgaagcct agggaggagt ccactctatg cgactactga
      481 tgagtccgtg aggacgaaac gagtaagctc gtcaaaccac aatttgcaca cccgttttag
      541 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      601 agtcggtgct tttggccggc atggtcccag cctcctcgct ggcgccggct gggcaacatg
      661 cttcggcatg gcgaatggga cggatcctaa ctcgagctct agctagagtc gatcgacaag
      721 ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat
      781 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
      841 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat
      901 gctgccaaac cagatgtcaa cacagctaca acgccaaacc acaatttgca caccctggca
      961 tctcaaacca caatttgcac accctggcat ctcaaaccac aatttgcaca ccctggcatc
     1021 tccgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cacgcgttta
     1081 tttacaagag cgtacggttc aatccctgcc tcccctgtaa aactaccctt tgaaaacctc
     1141 tctttcttaa tcttttcttt gtaattccag atctatggta tccaaaggag aagaattgtt
     1201 tacaggggta gtccccatat tggtcgagct cgatggggat gtaaacgggc acaagttctc
     1261 agtgtctggc gaaggcgaag gggacgccac atacggaaag ttgactctga agttcatctg
     1321 cacaaccggt aaactacccg taccctggcc aacactggtg acgacctttg ggtacgggct
     1381 acaatgtttc gctcgatatc ctgaccacat gaagcaacac gatttcttca aaagcgccat
     1441 gcctgagggg tatgtacagg agcgtaccat atttttcaaa gacgatggaa actataagac
     1501 ccgagctgaa gtgaaatttg aaggagatac gttagtcaat cgaatagaac taaagggaat
     1561 tgatttcaag gaggatggga atattctggg ccataagctg gagtacaatt acaatagtca
     1621 caacgtctat ataatggcag acaagcagaa gaatggaata aaggttaatt tcaagataag
     1681 gcataacatt gaagacggaa gcgttcagct agccgatcat taccaacaga atacaccgat
     1741 aggtgatgga cccgtcctgc tccccgacaa ccattacttg tcttaccaga gtgcactttc
     1801 taaagatcca aatgagaaaa gagatcatat ggttttatta gaatttgtta ccgcagccgg
     1861 cataactttg ggaatggacg aactgtacaa atgaggatcc taactcgagc tctagctaga
     1921 gtcgatcgac aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa
     1981 ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     2041 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta
     2101 ctaaaatcca gatgctggat gcacacactg gcttaagatg acaacgcgtc aacatggtgg
     2161 agcacgacac tctggtctac tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg
     2221 ctattgagac ttttcaacaa aggataattt cgggaaacct cctcggattc cattgcccag
     2281 ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc
     2341 attgcgataa aggaaaggct atcattcaag atctctctgc cgacagtggt cccaaagatg
     2401 gacccccacc cacgaggagc atcgtggaaa aagaagaggt tccaaccacg tctacaaagc
     2461 aagtggattg atgtgacatc tccactgacg taagggatga cgcacaatcc cactatcctt
     2521 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgcgtttatt
     2581 tacaagagcg tacggttcaa tccctgcctc ccctgtaaaa ctaccctttg aaaacctctc
     2641 tttcttaatc ttttctttgt aattccagat ctatgcccaa gaagaagagg aaggtggaca
     2701 agaagtactc cattgggctc gctatcggca caaacagcgt cggctgggcc gtcattacgg
     2761 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
     2821 taaagaagaa cctcattggc gccctcctgt tcgactccgg ggaaacggcc gaagccacgc
     2881 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
     2941 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
     3001 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
     3061 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg
     3121 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca
     3181 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca
     3241 aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaacg
     3301 catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg
     3361 aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg
     3421 ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca
     3481 agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg gcccagatcg
     3541 gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc attctgctga
     3601 gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct agtatgatca
     3661 agcgctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc agacagcaac
     3721 tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac gccggataca
     3781 ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc ttggaaaaaa
     3841 tggacggcac cgaggagctg ctggtaaagc ttaacagaga agatctgttg cgcaaacagc
     3901 gcactttcga caatggaagc atcccccacc agattcacct gggcgaactg cacgctatcc
     3961 tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag attgagaaaa
     4021 tcctcacatt tcggataccc tactatgtag gccccctcgc ccggggaaat tccagattcg
     4081 cgtggatgac tcgcaaatca gaagagacta tcactccctg gaacttcgag gaagtcgtgg
     4141 ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat aaaaatctgc
     4201 ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca gtttataacg
     4261 agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc ctgtctggag
     4321 agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt accgtgaaac
     4381 agctcaaaga agattatttc aaaaagattg aatgtttcga ctctgttgaa atcagcggag
     4441 tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa atcattaaag
     4501 acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt gtcctcaccc
     4561 ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac gctcatctct
     4621 tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata tacaggatgg gggcggctgt
     4681 caagaaaact gatcaatggg atccgagaca agcagagtgg aaagacaatc ctggattttc
     4741 ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat gactctctca
     4801 cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt ctccacgagc
     4861 acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag accgttaagg
     4921 tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc gttatcgaga
     4981 tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa aggatgaaga
     5041 ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac ccagttgaaa
     5101 acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc agggacatgt
     5161 acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggat gccatcgtgc
     5221 cccagtcttt tctcaaagat gattctattg ataataaagt gttgacaaga tccgataaaa
     5281 atagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg aaaaattatt
     5341 ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat ctgactaagg
     5401 ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg cagcttgttg
     5461 agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg aacaccaagt
     5521 acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag tctaagctgg
     5581 tttcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac aattaccacc
     5641 atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa aaatatccca
     5701 agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg aaaatgatcg
     5761 caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac agcaatatta
     5821 tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag cgaccactta
     5881 tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat ttcgcgacag
     5941 tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa gtacagaccg
     6001 gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg atcgcacgca
     6061 aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc gcttacagtg
     6121 tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc gtcaaggaac
     6181 tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc gactttctcg
     6241 aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc aagtactctc
     6301 tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag ctgcagaaag
     6361 gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc agccactatg
     6421 aaaagctcaa aggatctccc gaagataatg agcagaagca gctgttcgtg gaacaacaca
     6481 aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga gtgatcctcg
     6541 ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat aagcccatca
     6601 gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc gcgcctgcag
     6661 ccttcaagta cttcgacacc accatagaca gaaagcggta cacctctaca aaggaggtcc
     6721 tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga atcgacctct
     6781 ctcagctcgg tggagacagc agggctgatt cggacccaaa aaagaagcgt aaggtcgatc
     6841 ccaagaagaa gagaaaggta gatcctaaga agaagagaaa ggtagacgca ttggatgact
     6901 tcgacttaga catgcttggg tcagatgctt tggacgactt tgacctcgac atgttgggtt
     6961 ctgacgcgct agatgacttc gacttggaca tgttgggctc cgacgctttg gacgactttg
     7021 atctggacat gttatgagga tcctaactcg agctctagct agagtcgatc gacaagctcg
     7081 agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg
     7141 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
     7201 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatgctg
     7261 agcagttaca gagatgttac gaaccgggac acgaagtggt ttaaactatc agtgtttgac
     7321 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     7381 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgccaacca caggaaccgt
     7441 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7501 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7561 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7621 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7681 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7741 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7801 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7861 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     7921 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     7981 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8041 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8101 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8161 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8221 agctaaaaca attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc
     8281 cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac
     8341 cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata
     8401 aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa
     8461 aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct
     8521 ttaaatggag tgtcctcttc ccagttttcg caatccacat cggccagatc gttattcagt
     8581 aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg
     8641 tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt
     8701 tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg
     8761 ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc
     8821 catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc
     8881 gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac
     8941 attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat
     9001 attctcattt tagccatact cttccttttt caatattatt gaagcattta tcagggttat
     9061 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9121 cgcacgaatt ggccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca
     9181 gcccctgggg ggatgggagc tcggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     9241 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     9301 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     9361 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     9421 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     9481 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     9541 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
     9601 gaaagaagct ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     9661 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     9721 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     9781 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     9841 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     9901 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     9961 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
    10021 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
    10081 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
    10141 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
    10201 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
    10261 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
    10321 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
    10381 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
    10441 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
    10501 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
    10561 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
    10621 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
    10681 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgtcacgcc
    10741 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt
    10801 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
    10861 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
    10921 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
    10981 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
    11041 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
    11101 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
    11161 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
    11221 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
    11281 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
    11341 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
    11401 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
    11461 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
    11521 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
    11581 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
    11641 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
    11701 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
    11761 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
    11821 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
    11881 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
    11941 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggct gatgggctgc
    12001 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
    12061 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
    12121 cgtttttaat gtactggggt ggatgcagtg ggccccactc tgtgtgccga attcggatcc
    12181 ggagagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg ggacaagccg
    12241 ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc gcgggtttct
    12301 ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca aaagtcgcct
    12361 aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac gttccataaa
    12421 ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa tctgcaccgg
    12481 atctggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
    12541 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
    12601 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
    12661 tgccctgaat gaactccagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
    12721 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
    12781 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
    12841 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
    12901 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
    12961 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
    13021 ggcgcgtatg cccgacggcg aggatctcgt cgtgactcat ggcgatgcct gcttgccgaa
    13081 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
    13141 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
    13201 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
    13261 cttctatcgc cttcttgacg agttcttctg agcgggactc tggatctaga gtcaagcaga
    13321 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
    13381 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
    13441 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tctaatacgc
    13501 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
    13561 gttactagat cgacgctgca agaattcaag ct
//
