LOCUS       OCS_1-1                13694 bp    DNA     circular UNA 06-JUN-2021
DEFINITION  Golden Gate/Type IIS Cloning ligation product.
ACCESSION   urn.local...1rf-dfpttvt
VERSION     urn.local...1rf-dfpttvt
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    6..1010
                     /Source="referenceToDocument: urn:local:.:1q7-dfptpm6"
                     /label="TU1-1 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    6..30
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK002"
                     /Transferred_Similarity="100.00%"
                     /label="ConS scar"
     misc_feature    36..540
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /label="U6 promoter paper"
     misc_feature    541..560
                     /created_by="User"
                     /label="gRNA-1"
     misc_feature    561..640
                     /ApEinfo_revcolor="#4764ff"
                     /ApEinfo_fwdcolor="#4764ff"
                     /label="gRNA Handle"
     misc_feature    641..1002
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /label="U6 terminator paper"
     misc_feature    1007..2223
                     /Source="referenceToDocument: urn:local:.:1qi-dfptqs4"
                     /label="TU2-1 (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1007..1031
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK003"
                     /Transferred_Similarity="100.00%"
                     /label="Con1 scar"
     promoter        1037..1170
                     /created_by="User"
                     /modified_by="User"
                     /label="ATF1-2 promoter"
     Misc._binding   1038..1057
                     /created_by="User"
                     /label="gRNA1-target"
     Misc._binding   1067..1086
                     /created_by="User"
                     /label="gRNA1-target"
     Misc._binding   1096..1115
                     /created_by="User"
                     /label="gRNA1-target"
     promoter        <1125..1170
                     /modified_by="User"
                     /label="minimal 35S"
     Misc._binding   1171..1177
                     /label="CaMV 5' UTL"
     Misc._binding   1171
                     /label="Start of transcription"
     5'UTR           1178..1270
                     /modified_by="User"
                     /label="CMV1 5'UTR"
     CDS             1277..1996
                     /created_by="User"
                     /label="YFP"
     terminator      2012..2215
                     /label="35S ter"
     misc_feature    2220..7366
                     /Source="referenceToDocument: urn:local:.:1qt-dfptrdc"
                     /label="TU3E-1 (concatenated sequence 4)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    2224..2244
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK004"
                     /Transferred_Similarity="100.00%"
                     /label="Con2 scar"
     promoter        2250..2668
                     /modified_by="User"
                     /label="CaMV 35 S promoter (short)"
     Misc._binding   2669..2675
                     /label="CaMV 5' UTL"
     Misc._binding   2669
                     /label="Start of transcription"
     5'UTR           2676..2768
                     /modified_by="User"
                     /label="CMV1 5'UTR"
     source          <2775..>7136
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     CDS             2778..2798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             2799..6899
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II
                     CRISPR/Cas system"
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC
                     catalytic domain and the H840A mutation in the HNH
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
                     SFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHM
                     IKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKS
                     RRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDN
                     LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLL
                     KALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKL
                     NREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY
                     VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP
                     KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKED
                     YFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTL
                     FEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFL
                     KSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTV
                     KVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEH
                     PVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVL
                     TRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAG
                     FIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFY
                     KVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSM
                     PQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVA
                     KVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFE
                     LENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHK
                     HYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAP
                     AAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /label="dCas9"
     CDS             6918..6938
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             6942..6962
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             6966..6986
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             6987..7136
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDML"
                     /label="VP64"
     terminator      7155..7358
                     /label="35S ter"
     misc_feature    7363..9
                     /Source="referenceToDocument: urn:local:.:1r3-dfptt38"
                     /label="pAPT.Ex.KanR Copy (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    7363..7387
                     /ApEinfo_fwdcolor="#FFFD6C"
                     /ApEinfo_revcolor="#FFFD6C"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /Transferred_From="pYTK073, pYTK096"
                     /Transferred_Similarity="100.00%"
                     /label="ConE scar"
     misc_feature    complement(7405..7532)
                     /label="RB short"
     rep_origin      complement(7551..8165)
                     /modified_by="User"
                     /label="rep"
     rep_origin      7566
                     /label="ORI"
     CDS             complement(8328..9119)
                     /label="Kan (no BpiI)"
     mutation        8637
                     /label="A to G (BpiI removed)"
     rep_origin      complement(9305..12089)
                     /modified_by="User"
                     /label="pVS1ori short"
     misc_feature    12091..12238
                     /label="LB"
     misc_feature    12287..12586
                     /label="nos prom"
                     /note="Geneious type: promoter uukaryotic"
     CDS             12599..13390
                     /modified_by="User"
                     /label="KanR"
     terminator      13408..13675
                     /label="nos term"
ORIGIN      
        1 ctagactgaa ctggccgata attgcagacg aacgttcgac gtaaagcctg tagaagaggt
       61 ttctagcgaa cgacacgagt ttgagcctca tgaagcttcg ttgaacaacg gaaactcgac
      121 ttgccttccg cacaatacat catttcttct tagctttttt tcttcttctt cgttcataca
      181 gttttttttt gtttatcagc ttacattttc ttgaaccgta gctttcgttt tcttcttttt
      241 aactttccat tcggagtttt tgtatcttgt ttcatagttt gtcccaggat tagaatgatt
      301 aggcatcgaa ccttcaagaa tttgattgaa taaaacatct tcattcttaa gatatgaaga
      361 taatcttcaa aaggcccctg ggaatctgaa agaagagaag caggcccatt tatatgggaa
      421 agaacaatag tatttcttat ataggcccat ttaagttgaa aacaatcttc aaaagtccca
      481 catcgcttag ataagaaaac gaagctgagt ttatatacag ctagagtcga agtagtgatt
      541 aaaccacaat ttgcacaccc gttttagagc tagaaatagc aagttaaaat aaggctagtc
      601 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttgcaaaat tttccagatc
      661 gatttcttct tcctctgttc ttcggcgttc aatttctggg tttttctctt cgttttctgt
      721 aactgaaacc taaaatttga cctaaaaaaa atctcaaata atatgattca gtggttttgt
      781 acttttcagt tagttgagtt ttgcagttcc gatgagataa accaataact ttgcttagat
      841 ctaattcatt ccgttacacc tctgatggag atggaaggtt cttaataatg atgccatttt
      901 ttgggtaata attttgaatt agaatcaagg gtataagatt cataattaac atcacttaag
      961 caaagttcgt aatatacgac cacaggatat aatttttggt acgctgccaa accagatgtc
     1021 aacacagcta caacgccaaa ccacaatttg cacaccctgg catctcaaac cacaatttgc
     1081 acaccctggc atctcaaacc acaatttgca caccctggca tctccgcaag acccttcctc
     1141 tatataagga agttcatttc atttggagag gacacgcgtt tatttacaag agcgtacggt
     1201 tcaatccctg cctcccctgt aaaactaccc tttgaaaacc tctctttctt aatcttttct
     1261 ttgtaattcc agatctatgg tatccaaagg agaagaattg tttacagggg tagtccccat
     1321 attggtcgag ctcgatgggg atgtaaacgg gcacaagttc tcagtgtctg gcgaaggcga
     1381 aggggacgcc acatacggaa agttgactct gaagttcatc tgcacaaccg gtaaactacc
     1441 cgtaccctgg ccaacactgg tgacgacctt tgggtacggg ctacaatgtt tcgctcgata
     1501 tcctgaccac atgaagcaac acgatttctt caaaagcgcc atgcctgagg ggtatgtaca
     1561 ggagcgtacc atatttttca aagacgatgg aaactataag acccgagctg aagtgaaatt
     1621 tgaaggagat acgttagtca atcgaataga actaaaggga attgatttca aggaggatgg
     1681 gaatattctg ggccataagc tggagtacaa ttacaatagt cacaacgtct atataatggc
     1741 agacaagcag aagaatggaa taaaggttaa tttcaagata aggcataaca ttgaagacgg
     1801 aagcgttcag ctagccgatc attaccaaca gaatacaccg ataggtgatg gacccgtcct
     1861 gctccccgac aaccattact tgtcttacca gagtgcactt tctaaagatc caaatgagaa
     1921 aagagatcat atggttttat tagaatttgt taccgcagcc ggcataactt tgggaatgga
     1981 cgaactgtac aaatgaggat cctaactcga gctctagcta gagtcgatcg acaagctcga
     2041 gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt
     2101 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     2161 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatgctgg
     2221 atgcacacac tggcttaaga tgacaacgcg tcaacatggt ggagcacgac actctggtct
     2281 actccaaaaa tgtcaaagat acagtctcag aagatcaaag ggctattgag acttttcaac
     2341 aaaggataat ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
     2401 aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg
     2461 ctatcattca agatctctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     2521 gcatcgtgga aaaagaagag gttccaacca cgtctacaaa gcaagtggat tgatgtgaca
     2581 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
     2641 tataaggaag ttcatttcat ttggagagga cacgcgttta tttacaagag cgtacggttc
     2701 aatccctgcc tcccctgtaa aactaccctt tgaaaacctc tctttcttaa tcttttcttt
     2761 gtaattccag atctatgccc aagaagaaga ggaaggtgga caagaagtac tccattgggc
     2821 tcgctatcgg cacaaacagc gtcggctggg ccgtcattac ggacgagtac aaggtgccga
     2881 gcaaaaaatt caaagttctg ggcaataccg atcgccacag cataaagaag aacctcattg
     2941 gcgccctcct gttcgactcc ggggaaacgg ccgaagccac gcggctcaaa agaacagcac
     3001 ggcgcagata tacccgcaga aagaatcgga tctgctacct gcaggagatc tttagtaatg
     3061 agatggctaa ggtggatgac tctttcttcc ataggctgga ggagtccttt ttggtggagg
     3121 aggataaaaa gcacgagcgc cacccaatct ttggcaatat cgtggacgag gtggcgtacc
     3181 atgaaaagta cccaaccata tatcatctga ggaagaagct tgtagacagt actgataagg
     3241 ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat caaatttcgg ggacacttcc
     3301 tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga caaactcttt atccaactgg
     3361 ttcagactta caatcagctt ttcgaagaga acccgatcaa cgcatccgga gttgacgcca
     3421 aagcaatcct gagcgctagg ctgtccaaat cccggcggct cgaaaacctc atcgcacagc
     3481 tccctgggga gaagaagaac ggcctgtttg gtaatcttat cgccctgtca ctcgggctga
     3541 cccccaactt taaatctaac ttcgacctgg ccgaagatgc caagcttcaa ctgagcaaag
     3601 acacctacga tgatgatctc gacaatctgc tggcccagat cggcgaccag tacgcagacc
     3661 tttttttggc ggcaaagaac ctgtcagacg ccattctgct gagtgatatt ctgcgagtga
     3721 acacggagat caccaaagct ccgctgagcg ctagtatgat caagcgctat gatgagcacc
     3781 accaagactt gactttgctg aaggcccttg tcagacagca actgcctgag aagtacaagg
     3841 aaattttctt cgatcagtct aaaaatggct acgccggata cattgacggc ggagcaagcc
     3901 aggaggaatt ttacaaattt attaagccca tcttggaaaa aatggacggc accgaggagc
     3961 tgctggtaaa gcttaacaga gaagatctgt tgcgcaaaca gcgcactttc gacaatggaa
     4021 gcatccccca ccagattcac ctgggcgaac tgcacgctat cctcaggcgg caagaggatt
     4081 tctacccctt tttgaaagat aacagggaaa agattgagaa aatcctcaca tttcggatac
     4141 cctactatgt aggccccctc gcccggggaa attccagatt cgcgtggatg actcgcaaat
     4201 cagaagagac tatcactccc tggaacttcg aggaagtcgt ggataagggg gcctctgccc
     4261 agtccttcat cgaaaggatg actaactttg ataaaaatct gcctaacgaa aaggtgcttc
     4321 ctaaacactc tctgctgtac gagtacttca cagtttataa cgagctcacc aaggtcaaat
     4381 acgtcacaga agggatgaga aagccagcat tcctgtctgg agagcagaag aaagctatcg
     4441 tggacctcct cttcaagacg aaccggaaag ttaccgtgaa acagctcaaa gaagattatt
     4501 tcaaaaagat tgaatgtttc gactctgttg aaatcagcgg agtggaggat cgcttcaacg
     4561 catccctggg aacgtatcac gatctcctga aaatcattaa agacaaggac ttcctggaca
     4621 atgaggagaa cgaggacatt cttgaggaca ttgtcctcac ccttacgttg tttgaagata
     4681 gggagatgat tgaagaacgc ttgaaaactt acgctcatct cttcgacgac aaagtcatga
     4741 aacagctcaa gaggcgccga tatacaggat gggggcggct gtcaagaaaa ctgatcaatg
     4801 ggatccgaga caagcagagt ggaaagacaa tcctggattt tcttaagtcc gatggatttg
     4861 ccaaccggaa cttcatgcag ttgatccatg atgactctct cacctttaag gaggacatcc
     4921 agaaagcaca agtttctggc cagggggaca gtctccacga gcacatcgct aatcttgcag
     4981 gtagcccagc tatcaaaaag ggaatactgc agaccgttaa ggtcgtggat gaactcgtca
     5041 aagtaatggg aaggcataag cccgagaata tcgttatcga gatggcccga gagaaccaaa
     5101 ctacccagaa gggacagaag aacagtaggg aaaggatgaa gaggattgaa gagggtataa
     5161 aagaactggg gtcccaaatc cttaaggaac acccagttga aaacacccag cttcagaatg
     5221 agaagctcta cctgtactac ctgcagaacg gcagggacat gtacgtggat caggaactgg
     5281 acatcaatcg gctctccgac tacgacgtgg atgccatcgt gccccagtct tttctcaaag
     5341 atgattctat tgataataaa gtgttgacaa gatccgataa aaatagaggg aagagtgata
     5401 acgtcccctc agaagaagtt gtcaagaaaa tgaaaaatta ttggcggcag ctgctgaacg
     5461 ccaaactgat cacacaacgg aagttcgata atctgactaa ggctgaacga ggtggcctgt
     5521 ctgagttgga taaagccggc ttcatcaaaa ggcagcttgt tgagacacgc cagatcacca
     5581 agcacgtggc ccaaattctc gattcacgca tgaacaccaa gtacgatgaa aatgacaaac
     5641 tgattcgaga ggtgaaagtt attactctga agtctaagct ggtttcagat ttcagaaagg
     5701 actttcagtt ttataaggtg agagagatca acaattacca ccatgcgcat gatgcctacc
     5761 tgaatgcagt ggtaggcact gcacttatca aaaaatatcc caagcttgaa tctgaatttg
     5821 tttacggaga ctataaagtg tacgatgtta ggaaaatgat cgcaaagtct gagcaggaaa
     5881 taggcaaggc caccgctaag tacttctttt acagcaatat tatgaatttt ttcaagaccg
     5941 agattacact ggccaatgga gagattcgga agcgaccact tatcgaaaca aacggagaaa
     6001 caggagaaat cgtgtgggac aagggtaggg atttcgcgac agtccggaag gtcctgtcca
     6061 tgccgcaggt gaacatcgtt aaaaagaccg aagtacagac cggaggcttc tccaaggaaa
     6121 gtatcctccc gaaaaggaac agcgacaagc tgatcgcacg caaaaaagat tgggacccca
     6181 agaaatacgg cggattcgat tctcctacag tcgcttacag tgtactggtt gtggccaaag
     6241 tggagaaagg gaagtctaaa aaactcaaaa gcgtcaagga actgctgggc atcacaatca
     6301 tggagcgatc aagcttcgaa aaaaacccca tcgactttct cgaggcgaaa ggatataaag
     6361 aggtcaaaaa agacctcatc attaagcttc ccaagtactc tctctttgag cttgaaaacg
     6421 gccggaaacg aatgctcgct agtgcgggcg agctgcagaa aggtaacgag ctggcactgc
     6481 cctctaaata cgttaatttc ttgtatctgg ccagccacta tgaaaagctc aaaggatctc
     6541 ccgaagataa tgagcagaag cagctgttcg tggaacaaca caaacactac cttgatgaga
     6601 tcatcgagca aataagcgaa ttctccaaaa gagtgatcct cgccgacgct aacctcgata
     6661 aggtgctttc tgcttacaat aagcacaggg ataagcccat cagggagcag gcagaaaaca
     6721 ttatccactt gtttactctg accaacttgg gcgcgcctgc agccttcaag tacttcgaca
     6781 ccaccataga cagaaagcgg tacacctcta caaaggaggt cctggacgcc acactgattc
     6841 atcagtcaat tacggggctc tatgaaacaa gaatcgacct ctctcagctc ggtggagaca
     6901 gcagggctga ttcggaccca aaaaagaagc gtaaggtcga tcccaagaag aagagaaagg
     6961 tagatcctaa gaagaagaga aaggtagacg cattggatga cttcgactta gacatgcttg
     7021 ggtcagatgc tttggacgac tttgacctcg acatgttggg ttctgacgcg ctagatgact
     7081 tcgacttgga catgttgggc tccgacgctt tggacgactt tgatctggac atgttatgag
     7141 gatcctaact cgagctctag ctagagtcga tcgacaagct cgagtttctc cataataatg
     7201 tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag
     7261 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     7321 taattcctaa aaccaaaatc cagtactaaa atccagatgc tgagcagtta cagagatgtt
     7381 acgaaccggg acacgaagtg gtttaaacta tcagtgtttg acaggatata ttggcgggta
     7441 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     7501 ttatccgttc gtccatttgt atgtgccaac cacaggaacc gtaaaaaggc cgcgttgctg
     7561 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7621 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7681 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7741 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7801 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7861 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7921 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7981 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     8041 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8101 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8161 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8221 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8281 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctaaaa caattcatcc
     8341 agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat
     8401 agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg
     8461 tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca
     8521 tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg
     8581 ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcctct
     8641 tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct
     8701 aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc
     8761 ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct
     8821 tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt
     8881 tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt
     8941 tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg
     9001 ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt
     9061 acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat tttagccata
     9121 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9181 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacgaa ttggccagcg
     9241 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
     9301 gctcggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc
     9361 ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc
     9421 gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct
     9481 cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa
     9541 taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt
     9601 acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc
     9661 caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaag ctttttcgac
     9721 ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg
     9781 atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg
     9841 cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga
     9901 aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc
     9961 atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc
    10021 cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg
    10081 tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt
    10141 cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc
    10201 ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc
    10261 aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta
    10321 cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc
    10381 ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac
    10441 tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga
    10501 tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc
    10561 tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt
    10621 cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt
    10681 tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc
    10741 gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg
    10801 cgccgatttg taccgggccg gatggtttgc gaccgtcacg ccgattcctc gggcttgggg
    10861 gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc
    10921 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
    10981 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
    11041 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
    11101 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
    11161 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
    11221 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
    11281 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
    11341 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
    11401 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
    11461 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
    11521 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
    11581 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
    11641 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
    11701 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
    11761 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
    11821 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
    11881 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
    11941 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
    12001 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
    12061 aaccggccag gatttcatgc agccgcacgg ctgatgggct gcctgtatcg agtggtgatt
    12121 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg
    12181 taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta atgtactggg
    12241 gtggatgcag tgggccccac tctgtgtgcc gaattcggat ccggagagcg gagaattaag
    12301 ggagtcacgt tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga
    12361 cagaaccgca acgttgaagg agccactcag ccgcgggttt ctggagttta atgagctaag
    12421 cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag
    12481 caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat
    12541 tagagtctca tattcactct caatccaaat aatctgcacc ggatctggat cgtttcgcat
    12601 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
    12661 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
    12721 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactcca
    12781 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
    12841 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
    12901 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
    12961 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
    13021 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
    13081 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgta tgcccgacgg
    13141 cgaggatctc gtcgtgactc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
    13201 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
    13261 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
    13321 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
    13381 cgagttcttc tgagcgggac tctggatcta gagtcaagca gatcgttcaa acatttggca
    13441 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
    13501 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
    13561 ggtttttatg attagagtcc cgcaattata catctaatac gcgatagaaa acaaaatata
    13621 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgacgctg
    13681 caagaattca agct
//
