%FLIM SPCImage Processing Code

%Original: Quinn Lab

%Adapted by: Shelby Bess

%Date: March 31, 2022

%Import a1 and a2 values

a1=importdata('01-FLIM-001_a1.asc');

a2=importdata('01-FLIM-001_a2.asc');

%Normalize a1 and a2 values

total=a1+a2;

A1=a1./total;

A2=a2./total;

%Import t1 and t2

t1=importdata('01-FLIM-001_t1.asc');

t2=importdata('01-FLIM-001_t2.asc');

%Calculate mean lifetime and create image

tauM=(A1.*t1)+(A2.*t2);

figure; imagesc(tauM); title('Mean Lifetime [ns]'); colormap(flipud(jet));

caxis([0 3000]); axis image; axis off;

c=colorbar;

%Calculate A1/A2 ratio

AR=A1./A2;

figure; imagesc(AR); title('A1/A2'); colormap(flipud(jet));

caxis([0 10]); axis image; axis off;

d=colorbar;

Error using importdata

Unable to open file.

Error in FLIMProcessing (line 7)

a1=importdata('01-FLIM-001_a1.asc');

Published with MATLAB® R2023b

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