{"text_format":"3","workbook":"xls","n_sheets":6,"sheets":["Primers1  Table S1","Primers2 TABEL S2","table S3","Supplementary Table S4","m6A modified peaks Table S5","外泌体数据"],"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Primers1  Table S1","index":0,"n_rows":28,"n_cols":3,"rows_kept":28,"merged":[[0,0,0,2],[1,0,1,1]],"flags":["formulas_cached_only"]}
["Table S1. qPCR primers used for detecting expression of indicated  molecules"]
["Gene Name",null,"Sequence (5'->3')"]
["ALKBH5:","Forward primer","GCGGTCATCAACGACTACCA"]
[null,"Reverse primer","AACTTGCAGCCGAAGCAAAG"]
["EIF3:","Forward primer","GACGTGGTCCCGATGAAGAT"]
[null,"Reverse primer","ATCACCAATTCGCCTGGGAG"]
["FTO:","Forward primer","TCTTCACCAGGGAGACTGCTA"]
[null,"Reverse primer","CCACACGGTGAGTGGAACTA"]
["METTL14:","Forward primer","ATTGCAGCACCTCGGTCATT"]
[null,"Reverse primer","ACCCCACTTTCGCAAGCATA"]
["METTL3:","Forward primer","AACATCTGTGGCCCCTGAAC"]
[null,"Reverse primer","TGGCGTAGAGATGGCAAGAC"]
["Ythdc1：","Forward primer","ACAGCCGGGAGGAGAAAGAT"]
[null,"Reverse primer","ACAATTCATCATCCTGTTCTGGT"]
["Ythdc2：","Forward primer","GGGGCTTTACGAGCCAAGTA"]
[null,"Reverse primer","TGTCCAAAGTGCTTAGCCCAA"]
["Ythdf3 ：","Forward primer","GCCACTAGCGTGGATCAGAG"]
[null,"Reverse primer","GCTGTTATTCTGATTTGTCTGGCT"]
["Ythdf2：","Forward primer","CAGCTCTCAGTCCAGCAACA"]
[null,"Reverse primer","AGTAGATCCAGAACCCGCCT"]
["Ythdf1：","Forward primer","CTGCAGTTAAGACGGTGGGT"]
[null,"Reverse primer","TAGCAATGGCTGCCCATGAA"]
["Wtap：","Forward primer","GAGTGTGGCCCAACTGAGAT"]
[null,"Reverse primer","TCAGGCGTAAACTTCCAGGC"]
["Hnrnph2 ：","Forward primer","TGAAAGAATGAGGCGGGGTG"]
[null,"Reverse primer","AGACCCAAAACCATACCCGT"]
["GAPDH ：","Forward primer","TGAAATGTGCACGCACCAAG"]
[null,"Reverse primer","GGGAAGCAGCATTCAGGTCT"]

{"sheet":"Primers2 TABEL S2","index":1,"n_rows":30,"n_cols":4,"rows_kept":30,"merged":[[0,0,0,3],[1,1,1,2]],"flags":["formulas_cached_only"]}
["Table S2. qPCR primers used for detecting indicated M6A-IP production"]
["Number","Gene Name",null,"Sequence (5'->3')"]
[1,"ADAM17","Forward primer","TGATGTTCTGAATCGTGGAT"]
[null,null,"Reverse primer","GGTGGCGTGATAAGGATG"]
[2,"ADAM17","Forward primer","TGCCAATGACTCTTCACTC"]
[null,null,"Reverse primer","TGCTTCCTCCACTCCTT"]
[3,"ADAM17","Forward primer","GGAGATGCTGAAGATGACA"]
[null,null,"Reverse primer","AGTGGACAAGAATGCTGAA"]
[4,"ADAM17","Forward primer","ACTACGACATCCTCTCCTTA"]
[null,null,"Reverse primer","CAGACTTATACACCAGCATTC"]
[5,"ADAM17","Forward primer","AGAGACCTGCCTACACTTA"]
[null,null,"Reverse primer","GCTGAGAATGAGACCAACT"]
[6,"p65","Forward primer","TCCTGCCACTAACATACAC"]
[null,null,"Reverse primer","GTCATCACTCCACTCTACC"]
[7,"p65","Forward primer","CCTTGACCTTCCATAATAGTTC"]
[null,null,"Reverse primer","TCACTCCACTCTACCTCAG"]
[8,"p65","Forward primer","AGCGTCAGTCTTCTCGTA"]
[null,null,"Reverse primer","TGGTTCTCTTCAGGCATCT"]
[9,"p65","Forward primer","CAAGTCCGAGAAGGTGAA"]
[null,null,"Reverse primer","GAATGGTGAAGCAAGAAGG"]
[10,"TNF-a","Forward primer","TGTCCATTCCTGAGTTCTG"]
[null,null,"Reverse primer","GGAGGCAACAAGGTAGAG"]
[11,"TNF-a","Forward primer","TCAGAATGAGGCTGGATAAG"]
[null,null,"Reverse primer","AGAGGAGGCAACAAGGTA"]
[12,"TNF-a","Forward primer","ACCACCATCAAGGACTCA"]
[null,null,"Reverse primer","AGGTCTGAAGGTAGGAAGG"]
[13,"TNF-a","Forward primer","AGACACCATGAGCACAGA"]
[null,null,"Reverse primer","CCACAAGCAGGAATGAGAA"]
[14,"TNF-a","Forward primer","GGTGTTCATCCATTCTCTAC"]
[null,null,"Reverse primer","ATCTTATCCAGCCTCATTCT"]

{"sheet":"table S3","index":2,"n_rows":14,"n_cols":10,"rows_kept":14,"merged":[[0,0,0,9]],"flags":["formulas_cached_only"]}
["Table S3. Statistical analysis of reads mapped in reference genome"]
["Sample","Total reads","Total mapped reads","Multiple mapped","Uniquely mapped","Reads mapped to '+'","Reads mapped to '-'","Non-splice reads","Splice reads","Reads mapped in proper pairs"]
["LPS1 Input",4899759,"4466620(91.16%)","207990(4.66%)","4258629(95.34%)","2236377(52.51%)","2022252(47.49%)","2524527(59.28%)","1734102(40.72%)","3933037(80.27%)"]
["LPS2 Input",3804993,"3556146(93.46%)","221800(6.24%)","3334346(93.76%)","1801903(54.04%)","1532443(45.96%)","2154319(64.61%)","1180027(35.39%)","3026111(79.53%)"]
["LPS3 Input",4523924,"3948028(87.27%)","192789(4.88%)","3755239(95.12%)","1973092(52.54%)","1782147(47.46%)","2368004(63.06%)","1387235(36.94%)","3564400(78.79%)"]
["blank1 Input",4130096,"3786472(91.68%)","214444(5.66%)","3572028(94.34%)","1947652(54.53%)","1624376(45.47%)","2266674(63.46%)","1305354(36.54%)","3397830(82.27%)"]
["blank2 Input",4564408,"4067343(89.11%)","217085(5.33%)","3850258(94.66%)","1952957(50.72%)","1897301(49.28%)","2393568(62.17%)","1456690(37.83%)","3420568(74.94%)"]
["blank3 Input",5083262,"4527661(89.07%)","224874(4.96%)","4302787(95.03%)","2296282(53.37%)","2006505(46.63%)","2724159(63.31%)","1578628(36.69%)","4017302(79.03%)"]
["LPS1 IP",5722493,"5240086(91.57%)","232832(4.45%)","5007154(95.55%)","2560785(51.14%)","2446369(48.86%)","3606959(72.04%)","1400195(27.96%)","4811472(84.08%)"]
["LPS2 IP",5680839,"5145135(90.57%)","224294(4.36%)","4920841(95.64%)","2541790(51.65%)","2379051(48.35%)","3345944(68.00%)","1574897(32.00%)","4604888(81.06%)"]
["LPS3 IP",6421134,"5892032(91.76%)","229867(3.90%)","5662165(96.10%)","2916694(51.51%)","2745471(48.49%)","4004752(70.73%)","1657413(29.27%)","5252488(81.80%)"]
["blank1 IP",5058185,"4600419(90.95%)","245736(5.34%)","4354683(94.66%)","2226542(51.13%)","2128141(48.87%)","3143214(72.18%)","1211469(27.82%)","3942349(77.94%)"]
["blank2 IP",5881701,"5475863(93.10%)","239748(4.38%)","5236125(95.62%)","2649298(50.60%)","2586827(49.40%)","3423690(65.39%)","1812435(34.61%)","4702420(79.95%)"]
["blank3 IP",6636946,"6145148(92.59%)","328938(5.35%)","5816210(94.65%)","3233199(55.59%)","2583011(44.41%)","4217204(72.51%)","1599006(27.49%)","5435659(81.90%)"]

{"sheet":"Supplementary Table S4","index":3,"n_rows":4,"n_cols":2,"rows_kept":4,"merged":[[0,0,0,1]],"flags":["formulas_cached_only"]}
["Table S4. Differentially expressed genes of inflammatory response pathways by GEO2R analysis"]
["#Term","input_gene"]
["inflammatory response","Alox5,11689:down|S100a9,20202:down|Tlr11,239081:up|Serpina1a,20700:up|Il31ra,218624:up|Ahsg,11625:up|Ltb4r1,16995:down|Ednrb,13618:up|Irak2,108960:down|Sema7a,20361:down|Ndst1,15531:down|Mapk14,26416:up|Cxcr3,12766:down|Mapk8,26419:down|Esr1,13982:down|Krt16,16666:up|Hdac9,79221:up|Ccr6,12458:down|Cx3cr1,13051:down|Tnfrsf9,21942:down|Stat5b,20851:down|Il23a,83430:down|Gja1,14609:down|Il33,77125:up|Cd24a,12484:down|Pglyrp2,57757:up|Rictor,78757:up|Gper1,76854:down|Clec10a,17312:down|Adipoq,11450:up|Il4,16189:up|Pla2g7,27226:up|Cd46,17221:down|Kit,16590:up|F3,14066:down|Tnfsf18,240873:up|Il1rl1,17082:up|P2rx1,18436:down|Kng1,16644:up|Tnfrsf10b,21933:up|Prkcz,18762:down|Hp,15439:down|Fanca,14087:up|Tollip,54473:up|Ash1l,192195:down|Crp,12944:down|Lxn,17035:down|Ccr4,12773:down|Cxcr6,80901:up|Olr1,108078:down|Prkcq,18761:down|Mefv,54483:up|Wfdc1,67866:up|Plp1,18823:down|Tac4,93670:down|Slit2,20563:up|Plaa,18786:down|Sphk1,20698:down|Ccr1,12768:down|Pf4,56744:down|Pglyrp1,21946:down|Cela1,109901:down|Il1f6,54448:up"]
["regulation of inflammatory response","S100a9,20202:down|Tlr11,239081:up|Ahsg,11625:up|Fanca,14087:up|Ednrb,13618:up|Ash1l,192195:down|Sema7a,20361:down|Mapk14,26416:up|Esr1,13982:down|Cx3cr1,13051:down|Stat5b,20851:down|Wfdc1,67866:up|Mefv,54483:up|Il33,77125:up|Cd24a,12484:down|Pglyrp2,57757:up|Rictor,78757:up|Gper1,76854:down|Slit2,20563:up|Adipoq,11450:up|Il4,16189:up|Cd46,17221:down|Pglyrp1,21946:down|Tnfsf18,240873:up|Il1rl1,17082:up"]

{"sheet":"m6A modified peaks Table S5","index":4,"n_rows":16,"n_cols":5,"rows_kept":16,"merged":[[0,0,0,4]],"flags":["formulas_cached_only"]}
["Suppl. Table 3. m6A modification peaks in HMGB1, RELA, ADAM17, ADAM10 and TNF-α "]
["mRNAs in BV2 cells"]
["molecules ","log2(enrichment)\nin replicate1","log2(enrichment)\nin replicate2","position in mRNA ","RRACH motif"]
["HMGB1_peak 1",2.084306,2.312433,"380~554 ","none"]
["HMGB1_peak 2 ",2.200465,2.195342,"671~806 ","none"]
["HMGB1_peak 3",1.409912,1.852017,"1080~1252","none"]
["RELA_peak1 ",1.751289,2.203846,"443~569 ","none"]
["RELA_peak2 *",2.655012,2.893099,"712~988 ","GGACT"]
["ADAM17_peak1*",2.068462,2.369747,"112~229 ","GGACT"]
["ADAM17_peak2",2.457442,2.306746,"2461~2662","none"]
["ADAM17_peak3 ",1.102921,1.518573,"4225~4408 ","GGACT"]
["TNF-A_peak 1",1.202669,1.355931,"320~421","none"]
["TNF-A_peak 2*",1.909975,1.584957,"619~895 ","GGACT"]
["ADAM10_peak 1",1.301202,1.350532,"528~810","none"]
["ADAM10_peak 2",1.701985,1.782704,"719~1032","none"]
["* Highest enriched m6A RRACH motif"]

{"sheet":"外泌体数据","index":5,"n_rows":1,"n_cols":1,"rows_kept":1,"flags":["formulas_cached_only"]}
["打包文件"]