{"text_format":"3","workbook":"xlsx","n_sheets":3,"sheets":["Table S1","Table S2","Table S3"],"read":"whole","date_system":"1900"}

{"sheet":"Table S1","index":0,"n_rows":35,"n_cols":6,"rows_kept":35,"merged":[[0,0,0,5]]}
["Table S1. List of targets per vector construct. For each target, the gRNA sequence and genotyping primers are indicated. Vector sequences can be retrieved on the Gateway Vector website (https://gatewayvectors.vib.be) using specific Vector IDs."]
["Vector Name","Vector ID","Target Gene","Target sequence","Genotyping_F","Genotyping_R"]
["PcUBI::AsCas12a::G7T",null,"PDS3-1","CCAGCCATGGTCGGCGGTCAGGCT","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["PcUBI::FnCas12a::G7T",null,"PDS3-1","CCAGCCATGGTCGGCGGTCAGGCT","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["PcUBI::LbCas12a::G7T",null,"PDS3-1","CCAGCCATGGTCGGCGGTCAGGCT","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["PcUBI::AsCas12a::G7T",null,"PDS3-2","TCAGTCAAAGAATGGATGGAAAAG","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["PcUBI::FnCas12a::G7T",null,"PDS3-2","TCAGTCAAAGAATGGATGGAAAAG","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["PcUBI::LbCas12a::G7T",null,"PDS3-2","TCAGTCAAAGAATGGATGGAAAAG","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["PcUBI::AsCas12a::G7T",null,"PDS3-3","TCTGGCCATGTCAGCATCTCGTTG","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["PcUBI::FnCas12a::G7T",null,"PDS3-3","TCTGGCCATGTCAGCATCTCGTTG","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["PcUBI::LbCas12a::G7T",null,"PDS3-3","TCTGGCCATGTCAGCATCTCGTTG","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["PcUBI::SpCas9::G7T","9_18","PDS3","GGACTTTTGCCAGCCATGGT","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["PcUBI::SpCas9::G7T","9_18","GL1-1","GGAAAAGTTGTAGACTGAGA","ACAAGAAAGGTTTATGGACAGTTGA","TGTGCAACTCTTTTCTTCTTCAGA"]
["PcUBI::SpCas9::G7T","9_18","GL1-2","TTGAGCCCTAATGTGAACAA","ACAAGAAAGGTTTATGGACAGTTGA","TGTGCAACTCTTTTCTTCTTCAGA"]
["PcUBI::SpCas9::G7T","9_18","At2g22460","AAGGGACGAAGAGGAGCGAA","GCAAAGTCATAGCTTCAATGGAGT","ATCATCGAACCGGCCAAGTT"]
["PcUBI::SpCas9::G7T","9_18","IM1","TGCTCTCCTCCACCAACCAA","TCACTTGAGATGTGTGCAGAAGA","TGGTACACAGAATGGTGGAGA"]
["PcUBI::SpCas9::G7T","9_18","VAR1-1","TAACTCTGGTGTCATTGTGT","AGCTGGAGTGCCGTTCTTTT","TGCCCAGCCTCTGCAAATTA"]
["PcUBI::SpCas9::G7T","9_18","VAR1-2","TGAACTCTTCCGGCTACATC","AGCTGGAGTGCCGTTCTTTT","TGCCCAGCCTCTGCAAATTA"]
["PcUBI::SpCas9::G7T","9_18","TIR1","AGTGATGTTGACGACGTTAG","TCTCACATTGCTTCCACGTGA","AGCCGACTGCAAACCGAATA"]
["PcUBI::SpCas9::G7T","9_18","At4g12990","AGGCACACCTCTTCACTTAA","GCATGTAAGGATCGTGCTCT","ATTCTTAATGCAGCACCAATAAAGA"]
["PcUBI::SpCas9::G7T","9_18","ADH1","CTCAGGATCAACACCGAGCG","ACAGGGACAAACACCGTTGT","ACCAACAGCACCAAGACCAA"]
["ZmUBI::SpCas9::G7T",null,"PDS3","GGACTTTTGCCAGCCATGGT","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["RPS5P::SpCas9::G7T",null,"PDS3","GGACTTTTGCCAGCCATGGT","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["35SP::SpCas9::G7T",null,"PDS3","GGACTTTTGCCAGCCATGGT","GACGTCAGGAAGAACATGGTC","AACACCTCGTCGGTCACGC"]
["PcUBI::APOBEC1::Cas9D10A::G7T",null,"PDS3-7","CCTTCCATGCAGCTATCTGG","CAGTCTTGTGCTACACACCGC","CAAGAACATGAAGAGAGTATCAGTAACG"]
["PcUBI::APOBEC1::Cas9D10A::G7T",null,"PDS3-9","CTTTCCAGGGATCTGGTAAA","GTTGATTAACTTGTACTACCTCATCCC","CCTGCTAAAGAGTAGGTGATCATATG"]
["PcUBI::Cas9VQR::Pea3AT",null,"PPD2","ACACGTGTCAGCCGCCATT","TCCCTAAATCAGCACCAACCA","GCTTACTCGCGAAGATTGCC"]
["PcUBI::SpCas9::G7T","9_18","MC4-MC7_A3","ACTAAACATTATTGGCTTAG","CCACAATTTTCACGTGCCA","CTTCAATCAACGTGTCGTACG"]
["PcUBI::SpCas9::G7T","9_18","MC4-MC7_A9","GCAAAGAGAGCGTTGTTGAT","CCACAATTTTCACGTGCCA","CTTCAATCAACGTGTCGTACG"]
["PcUBI::SpCas9::G7T","9_18","MC4-MC7_A11","GTCGGAGAAAAAACTTCCGC","CCACAATTTTCACGTGCCA","CTTCAATCAACGTGTCGTACG"]
["PcUBI::SpCas9::G7T","9_18","MC4-MC7_B3","TCCTAGCCCTTGTAACCATT","CCACAATTTTCACGTGCCA","CTTCAATCAACGTGTCGTACG"]
["PcUBI::SpCas9::G7T","9_18","MC4-MC7_B7","CTCTGTTTTCAACAGATGAA","CCACAATTTTCACGTGCCA","CTTCAATCAACGTGTCGTACG"]
["pK LacI-Cas9",null,"OLE1","GGGTAACTTAAGTAGTGTGCTGG","TACGTAGTGCAACGCGAAGT","GCCGGTTTCCATAACTGGGA"]
["PcUBI::SpCas9::G7T",null,"PDS3-1 (N. benthamiana)","GCCGTTAATTTGAGAGTCCA","N.A.","N.A."]
["PcUBI::SpCas9::G7T",null,"PDS3-2 (N. benthamiana)","GAGATTGTTATTGCTGGTGC","N.A.","N.A."]

{"sheet":"Table S2","index":1,"n_rows":70,"n_cols":10,"rows_kept":70,"merged":[[0,0,0,9]]}
["Table S2. Complete list of generated vectors (entry, destination and expression). Vector sequences can be retrieved on the Gateway Vector website (https://gatewayvectors.vib.be) using specific Vector IDs. PGE ID is an internal reference ID. "]
["Vector ID","PGE ID","Name","Type","Category","Description","overhang","Bacterial selection","Plant selection","Reference"]
["N/A","PGE00013","pGGA000","Entry vector","Empty entry vector",null,"A-B","Ampicillin",null,"(Lampropoulos et al., 2013); Addgene ID 48856"]
["N/A","PGE00014","pGGB000","Entry vector","Empty entry vector",null,"B-C","Ampicillin",null,"(Lampropoulos et al., 2013); Addgene ID 48857"]
["N/A","PGE00015","pGGC000","Entry vector","Empty entry vector",null,"C-D","Ampicillin",null,"(Lampropoulos et al., 2013); Addgene ID 48858"]
["N/A","PGE00016","pGGD000","Entry vector","Empty entry vector",null,"D-E","Ampicillin",null,"(Lampropoulos et al., 2013); Addgene ID 48859"]
["N/A","PGE00017","pGGE000","Entry vector","Empty entry vector",null,"E-F","Ampicillin",null,"(Lampropoulos et al., 2013); Addgene ID 48860"]
["N/A","PGE00018","pGGF000","Entry vector","Variable linker",null,"F-G","Ampicillin",null,"(Lampropoulos et al., 2013); Addgene ID 48861"]
["N/A","PGE00289","pGG-B-Linker-C","Entry vector","Variable linker",null,"B-C","Ampicillin",null,"(Lampropoulos et al., 2013); Addgene ID 48821"]
["13_18","PGE03803","pGG-C-Linker-D","Entry vector","Variable linker",null,"C-D","Ampicillin",null,"(Blomme et al., 2021)"]
["N/A","PGE00285","pGG-D-Linker-E","Entry vector","Variable linker",null,"D-E","Ampicillin",null,"(Lampropoulos et al., 2013); Addgene ID 48834"]
["8_38","PGE00207","pGG-F-linkerII-G","Entry vector","Variable linker",null,"F-G","Gentamycin",null,"(Decaestecker et al., 2019)"]
["8_02","PGE00052","pGG-A-PcUBI-B","Entry vector","Promoter","PcUBI promoter","A-B","Ampicillin"]
["6_55","PGE00195","pGG-A-ZmUBI-B","Entry vector","Promoter","ZmUBI promoter","A-B","Ampicillin"]
["7_06","PGE01567","pGG-A-pRPS5A-B","Entry vector","Promoter","RPS5A promoter","A-B","Ampicillin"]
["7_03","PGE00288","pGG-A-p35S-B","Entry vector","Promoter","CaMV 35S promoter","A-B","Ampicillin",null,"(Lampropoulos et al., 2013); Addgene ID 48815"]
["8_46","PGE00283","pGG-B-OLE1-C","Entry vector","N-tag","Homology arm OLE1","B-C","Ampicillin",null,"(Decaestecker et al., 2019)"]
[null,"PGE00467","pGG-B-AsCas12a-PDS3-1-C","Entry vector","gRNA module",null,"B-C","Ampicillin"]
[null,"PGE00468","pGG-B-AsCas12a-PDS3-2-C","Entry vector","gRNA module",null,"B-C","Ampicillin"]
[null,"PGE00469","pGG-B-AsCas12a-PDS3-3-C","Entry vector","gRNA module",null,"B-C","Ampicillin"]
[null,"PGE00470","pGG-B-FnCas12a-PDS3-1-C","Entry vector","gRNA module",null,"B-C","Ampicillin"]
[null,"PGE00471","pGG-B-FnCas12a-PDS3-2-C","Entry vector","gRNA module",null,"B-C","Ampicillin"]
[null,"PGE00472","pGG-B-FnCas12a-PDS3-3-C","Entry vector","gRNA module",null,"B-C","Ampicillin"]
[null,"PGE00460","pGG-B-LbCas12a-PDS3-1-C","Entry vector","gRNA module",null,"B-C","Ampicillin"]
["16_69","PGE00461","pGG-B-LbCas12a-PDS3-2-C","Entry vector","gRNA module",null,"B-C","Ampicillin"]
[null,"PGE00462","pGG-B-LbCas12a-PDS3-3-C","Entry vector","gRNA module",null,"B-C","Ampicillin"]
[" 10_35","PGE00520","pGG-C-Cas9PTA-D","Entry vector","CDS","Cas9-NLS SV40","C-D","Ampicillin",null,"(Decaestecker et al., 2019)"]
["8_55","PGE00048","pGG-C-CAS9-D10A-D","Entry vector","CDS","nCas9 (D10A)","C-D","Ampicillin",null,"(Hu et al., 2019)"]
["N/A",null,"pY010",null,null,"AsCas12a",null,"Ampicillin",null,"Zetsche et al., 2015; Addgene ID 69982"]
["N/A",null,"pY004",null,null,"FnCas12a",null,"Ampicillin",null,"Zetsche et al., 2015; Addgene ID 69976"]
["N/A",null,"pY016",null,null,"LbCas12a ",null,"Ampicillin",null,"Zetsche et al., 2015; Addgene ID 69988"]
["16_70","PGE00369","pGG-C-LbCas12a-D","Entry vector","CDS",null,"C-D","Ampicillin"]
["16_71","PGE00368","pGG-C-AsCas12a-D","Entry vector","CDS",null,"C-D","Ampicillin"]
["16_72","PGE00367","pGG-C-FnCas12a-D","Entry vector","CDS",null,"C-D","Ampicillin"]
[null,"p00379","pFASTRK AsCas12a-AG","Destination vector",null,null,"A-G","Spectinomycin"]
[null,"p00380","pFASTRK-FnCas12a-AG","Destination vector",null,null,"A-G","Spectinomycin"]
[null,"p00381","pFASTRK-LbCas12a-AG","Destination vector",null,null,"A-G","Spectinomycin"]
["10_34","PGE00303","pGG-C-mRuby3-D","Entry vector","CDS",null,"C-D","Ampicillin",null,"(Decaestecker et al., 2019)"]
["16_73","PGE00519","pGG-D-OLE1 3'UTR-E","Entry vector","C-tag",null,"D-E","Ampicillin"]
["8_18","PGE01566","pGG-D-pea3T-G","Entry vector","Terminator",null,"D-G","Ampicillin"]
["8_54",null,"pGG-D-UGI-NLS-E","Entry vector","UGI",null,"D-E","Ampicillin",null,"(Hu et al., 2019)"]
["6_70","PGE00053","pGG-E-G7T-F","Entry vector","Terminator","G7 terminator","E-F","Ampicillin",null,"(Houbaert et al., 2018)"]
["9_48",null,"pFASTRK-AG","Destination vector",{"$nulls":3},"Spectinomycin",null,"(Decaestecker, Buono et al., 2019)"]
[null,"p00112","pEN-L4-ZmUBI-Cas9PTA-G7T-R1","Entry vector",null,null,"L4-R1","Kanamycin"]
[null,"p00113","pEN-L4-RPS5P-Cas9PTA-G7T-R1","Entry vector",null,null,"L4-R1","Kanamycin"]
[null,"p00114","pEN-L4-35SP-Cas9PTA-G7T-R1","Entry vector",null,null,"L4-R1","Kanamycin"]
["8_25",null,"pEN-L4-PcUBI-Cas9-G7T-R1 ","Entry vector",null,null,"L4-R1","Kanamycin",null,"(Houbaert et al., 2018)"]
["N/A",null,"pEn_Chimera ","Destination vector","gRNA module",null,"L1-L2","Kanamycin",null,"(Fauser et al., 2014)"]
["2_02",null,"pBm42GW,3 ","Destination vector",{"$nulls":3},"Spectinomycin",null,"(Karimi et al., 2007)"]
["9_18","p00284","pFASTRK-AtCas9-AtU6-Scaffold ","Destination vector",{"$nulls":3},"Spectinomycin",null,"this report"]
["9_17",null,"pFASTGK-AtCas9-AtU6-Scaffold ","Destination vector",{"$nulls":3},"Spectinomycin",null,"this report"]
["9_58",null,"pFASTRK24GW","Destination vector",{"$nulls":3},"Spectinomycin",null,"(Decaestecker et al., 2019)"]
["9_59",null,"pFASTGK24GW","Destination vector",{"$nulls":3},"Spectinomycin",null,"(Decaestecker et al., 2019)"]
["10_30",null,"pEN-L1-AtU6-26-BsaI-L2 ","Entry vector","gRNA module",null,null,"Kanamycin",null,"(Decaestecker et al., 2019)"]
[null,"p00619","pFASTRK-CBE ","Destination vector",{"$nulls":3},"Spectinomycin",null,"(Decaestecker et al., 2019)"]
[null,"PGE00137","ZmUBI::SpCas9::G7T","Expression vector",{"$nulls":3},"Spectinomycin","FAST Red; PPT"]
[null,"PGE00138","RPS5P::SpCas9::G7T","Expression vector",{"$nulls":3},"Spectinomycin","FAST Red; PPT"]
[null,"PGE00139","35SP::SpCas9::G7T","Expression vector",{"$nulls":3},"Spectinomycin","FAST Red; PPT"]
["16_74",null,"PcUBI::Cas9VQR::Pea3AT","Expression vector",{"$nulls":3},"Spectinomycin","Kanamycin","(Swinnen et al., 2020)"]
[null,"PGE00599","pK LacI-Cas9","Expression vector",{"$nulls":3},"Spectinomycin","Kanamycin","(Decaestecker, 2021)"]
["References"]
["Lampropoulos, A. et al. GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Plos One 8, 15 (2013)."]
["Houbaert, A. et al. POLAR-guided signaling complex assembly and localization drive asymmetric cell division. Nature 563, 574-578 (2018)."]
["Hu, Z. et al. Genome Editing-Based Engineering of CESA3 Dual Cellulose-Inhibitor-Resistant Plants. Plant Physiology 180 (2), 827-836 (2019)."]
["Decaestecker W., Buono et al. CRISPR-TSKO: A Technique for Efficient Mutagenesis in Specific Cell Types, Tissues, or Organs in Arabidopsis. Plant Cell 31 (12), 2868-2887 (2019)."]
["Swinnen G et al. Constitutive Steroidal Glycoalkaloid Biosynthesis in Tomato is Regulated by the Clade IIIe Basic Helix-Loop-Helix Transcription Factors MYC1 and MYC2. BiorXiv: https://doi.org/10.1101/2020.01.27.921833"]
["Blomme, J. et al., A molecular toolkit for the green seaweed Ulva mutabilis. Plant Physiology 10.1093/plphys/kiab185 (2021)."]
["Fauser, F. et al., Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. The Plant Journal 79 (2), 348-359 (2014)."]
["Karimi, M. et al., Recombinational Cloning with Plant Gateway Vectors. Plant Physiology 145, 1144-2254 (2007)."]
["Decaestecker, W., Optimization of CRISPR/Cas9 genome editing and the development of tools for tissue-specific knock-out, gene targeting and prime editing in Arabidopsis thaliana. Universiteit Gent. Faculteit Wetenschappen, 2021: http://hdl.handle.net/1854/LU-8710526"]

{"sheet":"Table S3","index":2,"n_rows":52,"n_cols":2,"rows_kept":52,"merged":[[0,0,0,1]]}
["Table S3. List of cloning primers and gBlock sequence."]
["BsaI-C-hFnCpf1F","TTTTGGTCTCAGGCTCCATGAGCATCTACCAGGAGTTCGTC"]
["BsaI-D-Cpf1R","TTTTGGTCTCACTGATTAGGCATAGTCGGGGACATCATATG"]
["BsaI-C-hAsCpf1F","TTTTGGTCTCAGGCTCCATGACACAGTTCGAGGGCTTTACC"]
["BsaI-C-hLbCpf1F","TTTTGGTCTCAGGCTCCATGAGCAAGCTGGAGAAGTTTACAAAC"]
["BsaI-C-HDVR","TTTTGGTCTCAAGCCGTCCCATTCGCCATGCCGAAG"]
["BsaI-B-HHF","TTTTGGTCTCAAACAAAATTACTGATGAGTCCGTGAGGAC"]
["LbPDS-1","TACTAAGTGTAGATCCAGCCATGGTCGGCGGTCAGGCT"]
["LbPDS-2","TACTAAGTGTAGATTCAGTCAAAGAATGGATGGAAAAG"]
["LbPDS-3","TACTAAGTGTAGATTCTGGCCATGTCAGCATCTCGTTG"]
["AsPDS-1","TACTCTTGTAGATCCAGCCATGGTCGGCGGTCAGGCT"]
["AsPDS-2","TACTCTTGTAGATTCAGTCAAAGAATGGATGGAAAAG"]
["AsPDS-3","TACTCTTGTAGATTCTGGCCATGTCAGCATCTCGTTG"]
["FnPDS-1","TACTGTTGTAGATCCAGCCATGGTCGGCGGTCAGGCT"]
["FnPDS-2","TACTGTTGTAGATTCAGTCAAAGAATGGATGGAAAAG"]
["FnPDS-3","TACTGTTGTAGATTCTGGCCATGTCAGCATCTCGTTG"]
["cpfPDS-1R","GGCCAGCCTGACCGCCGACCATGGCTGGATCTACACTT"]
["cpfPDS-2R","GGCCCTTTTCCATCCATTCTTTGACTGAATCTACACTT"]
["cpfPDS-3R","GGCCCAACGAGATGCTGACATGGCCAGAATCTACACTT"]
["HindIII-A-F","TCTGATCCAAGCTCAAGCTAAGCTTACCTTGAGACCGTCGAC"]
["PcUbi-GR","ATATTCATATCCGTAATTTTTAGGTAATACTGAGACCGCGGCC"]
["im-1F","ATTGTGCTCTCCTCCACCAACCAA"]
["im-2F","ATTGCACTGTCATGAAGTAGTAGA"]
["var1-1F","ATTGTAACTCTGGTGTCATTGTGT"]
["var1-2F","ATTGTGAACTCTTCCGGCTACATC"]
["var2-1F","ATTGGATATTCTCAAGGTTCATGC"]
["var2-2F","ATTGGCCAGCAACGATTCTATCAA"]
["AT4G12990-1F","ATTGAGGCACACCTCTTCACTTAA"]
["AT4G12990-2F","ATTGGTATTTAAACTCATTAGATT"]
["TIR1-1F","ATTGAGTGATGTTGACGACGTTAG"]
["TIR1-2F","ATTGCTCTGGAAAGGCTGGTGACT"]
["AT2G22460-1F","ATTGAAGGGACGAAGAGGAGCGAA"]
["AT2G22460-2F","ATTGCGAGCTAGCAGTTCCAACGA"]
["im-1R","AAACTTGGTTGGTGGAGGAGAGCA"]
["im-2R","AAACTCTACTACTTCATGACAGTG"]
["var1-1R","AAACACACAATGACACCAGAGTTA"]
["var1-2R","AAACGATGTAGCCGGAAGAGTTCA"]
["var2-1R","AAACGCATGAACCTTGAGAATATC"]
["var2-2R","AAACTTGATAGAATCGTTGCTGGC"]
["AT4G12990-1R","AAACTTAAGTGAAGAGGTGTGCCT"]
["AT4G12990-2R","AAACAATCTAATGAGTTTAAATAC"]
["TIR1-1R","AAACCTAACGTCGTCAACATCACT"]
["TIR1-2R","AAACAGTCACCAGCCTTTCCAGAG"]
["AT2G22460-1R","AAACTTCGCTCCTCTTCGTCCCTT"]
["AT2G22460-2R","AAACTCGTTGGAACTGCTAGCTCG"]
["gBlock","AAATTACTGATGAGTCCGTGAGGACGAAACGAGTAAGCTCGTCTAATTTCTACTGGGTCTTCGAGAAGACCTGGCCGGCATGGTCCCAGCCTCCTCGCTGGCGCCGGCTGGGCAACATGCTTCGGCATGGCGAATGGGAC"]
["PPD2-1F","ATTGAATGGCGGCTGACACGTGT"]
["PPD2-1R","AAACACACGTGTCAGCCGCCATT"]
["sgRNA-RV-B7-Mc4Mc7","TTTTGGTCTCAAAACTTCATCTGTTGAAAACAGAGCAATCACTACTTCGACTC"]
["sgRNA-FW-A9-Mc4Mc7","TTTTGGTCTCGATTGGCAAAGAGAGCGTTGTTGATGTTTTAGAGCTAGAAATAGC"]
["sgRNA-FW-A11-Mc4Mc7","TTTTGGTCTCGATTGGTCGGAGAAAAAACTTCCGCGTTTTAGAGCTAGAAATAGC"]
["sgRNA-FW-A3-Mc4Mc7","TTTTGGTCTCGATTGACTAAACATTATTGGCTTAGGTTTTAGAGCTAGAAATAGC"]