Table S1

Gene	Gene code	Primers (F: forward; R: reverse)
Clock	AAEL022593	F: 5’-CCGACGACAACGACTACAAA-3’ R: 5’-GTTCGGATTGCACAGTTCCT-3’
Cycle	AAEL002049	F: 5’-GAGCAGTTGTCTTCTTCGGATT-3’ R: 5’-CACAGCCTTGTCACACCTTG-3’
Period	AAEL008141	F: 5’-GGAGCAATTCGTCCGGTAG-3’ R: 5’-TGGGTTCAACAGACGTTTTC-3’
Timeless	AAEL019461	F: 5’-GGCTTTCTCGTACAAGAGCTTC-3’ R: 5’-GAACTTCAGGAAATAGGTCA-3’
Par domain protein1	AAEL005255	F: 5’-GACGCATGAAGGAGAATCAA-3’ R: 5’-TGGGTTCAACAGACGTTTTC-3’
Cryptochrome-1	AAEL004146	F: 5’-GCCGACGCTCTATGTCCACG-3’ R: 5’-CGATCATCGGTGCCGGATAGTC-3’
Cryptochrome-2	AAEL002602	F: 5’-GCAACTACGCCACAGTACGA-3’ R: 5’-GCTTCCGTTTCACCTCTCAC-3’

Table S2

Wildtype AeClk was subcloned between KpnI and XhoI using the following primers (F, forward; R: reverse)
F: 5’-TCGGGGTACCATGGAGGATGATCCGGATG-3’ R: 5’-TAGACTCGAGCATATCGCTCCTGGGGTGAC-3’
Wildtype AeCyc was subcloned between EcoRI and XbaI using
F: 5’-GGTGGAATTCATGATCTATTCAAAAAGTGCG-3’ R: 5’-CCCTCTAGACTCGACGAGTGGTAACGGC-3’
AeCyc-/- was subcloned between EcoRI and XhoI using
F: 5’-GGTGGAATTCATGATCTATTCAAAAAGTGCG-3’ R: 5’-TAGACTCGAGGCTCCTGGTCGGAGAGG-3’

Table S3a

The percentage of non-fed partially fed and fully fed mosquitoes in all the different time points are shown.

The standard error and the p-values (t-test) are also shown.

Mosquito fed at different time points (ZT)	Types of mosquito	Percentage of mosquitoes	Standard error	p-value (T-test) of total fed (with respect to wildtype)
Non-fed	Partially fed	Fully fed	Non-fed	Partially fed	Fully fed
1	AeCyc-/-	39	15	46	2.95	1.73	1.30	< 0.05; Significant
WT	64	8	28	1.79	0.55	1.52
6	AeCyc-/-	33	15	57	4.04	1.00	3.65	< 0.05; Significant
WT	56	12	26	1.79	0.89	1.10
11	AeCyc-/-	25	18	57	2.24	1.14	2.07	> 0.05; Non-significant
WT	36	18	46	1.48	2.19	1.92
16	AeCyc-/-	53	9	38	2.30	0.84	2.07	< 0.05; Significant
WT	82	4	14	0.55	0.84	0.84
21	AeCyc-/-	79	8	14	1.48	1.52	1.30	< 0.05; Significant
WT	94	2	3	1.30	0.55	0.89

Table S3b

Independent t-test between Cyc and WT groups at different time points.

The assumption of homogeneity of variances was tested and satisfied via Welch two sample t-test.

Mosquito fed at different time points (ZT)	Types of mosquito	t-test for Equality of Means
t	df	Welch’s t-test (P-value)
1	AeCyc-/-	3.24	6.59	0.015 (Significant)
WT
5	AeCyc-/-	5.1	4.81	0.003 (Significant)
WT
9	AeCyc-/-	1.83	6.94	0.109 (Non-significant)
WT
13	AeCyc-/-	5.48	4.45	0.003 (Significant)
WT
17	AeCyc-/-	5.013	7.86	0.001 (Significant)
WT